LOCUS       sequence-413892-        2847 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Type 0 promoter part .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Shapiro M
  TITLE     EcoFlex MoClo Extension, Volume I
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pBP-P_tetR-PTet*
FEATURES             Location/Qualifiers
     source          1..2847
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(169..263)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(284..943)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     858..877
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1105..1123
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1138..1177)
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     CDS             complement(1178..1801)
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     1709..1728
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     protein_bind    1906..1924
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1931..1949
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      1993..2050
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(2074..2091)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2245..2833)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2325..2344)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
       61 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
      121 aatctaaagt atatatgagt aaacttggtc tgacagctcg aggcttggat tctcaccaat
      181 aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat
      241 tactggatct atcaacagga gtccaagcga gctcgatatc aaattacgcc ccgccctgcc
      301 actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaaa
      361 cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt
      421 tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac
      481 tggtgaaact cacccaggga ttggctgaca cgaaaaacat attctcaata aaccctttag
      541 ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact
      601 gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga
      661 aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca
      721 tacgaaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa
      781 acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct
      841 ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt
      901 gggatatatc aacggtggta tatccagtga tttttttctc cattttagct tccttagctc
      961 ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa
     1021 agttggaacc tcttacgtgc ccgatcaact cgagtgccac ctgacgtcta agaaaccatt
     1081 attatcatga cattaaccta taaaaatagg cgtatcacga ggcagaggtc tcactattcc
     1141 aaaaaaaaag gctccaaaag gagcctttaa ttgtatctta ggacccactt tcacatttaa
     1201 gttgtttttc taatccgcat atgatcaatt caaggccgaa taagaaggct ggctctgcac
     1261 cttggtgatc aaataattcg atagcttgtc gtaataatgg cggcatacta tcagtagtag
     1321 gtgtttccct ttcttcttta gcgacttgat gctcttgatc ttccaatacg caacctaaag
     1381 taaaatgccc cacagcgctg agtgcatata atgcattctc tagtgaaaaa ccttgttggc
     1441 ataaaaaggc taattgattt tcgagagttt catactgttt ttctgtaggc cgtgtaccta
     1501 aatgtacttt tgctccatcg cgatgactta gtaaagcaca tctaaaactt ttagcgttat
     1561 tacgtaaaaa atcttgccag ctttcccctt ctaaagggca aaagtgagta tggtgcctat
     1621 ctaacatctc aatggctaag gcgtcgagca aagcccgctt attttttaca tgccaataca
     1681 atgtaggctg ctctacacct agcttctggg cgagtttacg ggttgttaaa ccttcgattc
     1741 cgacctcatt aagcagctct aatgcgctgt taatcacttt acttttatct aatctggaca
     1801 tattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt
     1861 tgcgccattc gatggcgcgc cgcttttcag caggacgcac tgacctccct atcagtgata
     1921 gagattgaca tccctatcag tgatagagat actgagcacg tacagagacc catgccgtct
     1981 cattagctgc agtccggcaa aaaagggcaa ggtgtcacca ccctgccctt tttctttaaa
     2041 accgaaaaga ttacttcgcg ttatgcaggc ttcctcgctc actgactcgc tgcgctcggt
     2101 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     2161 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     2221 taaaaaggcc gcgttgctgg cgtttttcca caggctccgc ccccctgacg agcatcacaa
     2281 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     2341 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     2401 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     2461 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     2521 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     2581 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     2641 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     2701 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     2761 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     2821 aaaaggatct caagaagatc ctttgat
//
