LOCUS       sequence-414035-       10312 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10312)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10312)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0231
FEATURES             Location/Qualifiers
     source          1..10312
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    3250..3838
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3303..3323)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3721..3732)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3910..4143
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4221..4342
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4258..4277)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4312..4331
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4382..4517
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4537..4556)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4538..4556)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4566..4583)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4566..4582)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4575..4597)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4724..5179
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4811..4830)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5021..5042
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5205..5309
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5310..5378,5379..6170)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5528..5547)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6341..6929
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6830..6849
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7083..7100
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7217..7238
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7253..7258,7259..7276,7277..7283)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7291..7307
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7296..7318
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7315..7331
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7315..7331
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7350..7370
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7352..7370
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7398..7624
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7625..7805
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7852..7977
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8470..8703
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8888..8932
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9163..9373
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9185..9381
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9232..9367
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9413..10015
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9413..9432)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9909..9929
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10058..10175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgga
     1201 actcgtaaca ttcagggatg tggccataga attctcccct gaagagtgga aatgtctgga
     1261 ccctgcccag cagaatttgt atagagatgt gatgttggag aactacagga acctggtctc
     1321 cctgggtttt gtgatctcta acccagacct ggtcacctgt ctggagcaaa taaaagagcc
     1381 ctgcaatttg aagatacatg agacagcagc caaaccccca gctatatgtt ctcctttcag
     1441 ccaagacctt tcaccagtgc aggggataga agattcattc cacaaactta tactgaaaag
     1501 atacgagaaa tgtggacatg agaatttaca attaagaaaa ggctgtaaac gtgtgaatga
     1561 gtgtaaggtg cagaaaggag ttaataatgg agtttaccag tgcttgtcaa ctacccagag
     1621 caaaatattt caatgtaata catgtgttaa agtttttagt aaattttcaa attcaaacaa
     1681 acataagata agacatactg gagagaaacc ctttaaatgt acagaatgtg gcagatcgtt
     1741 ttacatgtca cacctaactc aacatacagg aattcatgct ggagagaaac cctacaaatg
     1801 tgaaaaatgt ggcaaagcct ttaataggtc cacatcactt agtaaacata agagaattca
     1861 tactggagag aaaccctaca catgtgaaga atgtggcaaa gcctttagac ggtccacagt
     1921 tctgaacgaa cataagaaaa ttcatactgg agagaaaccc tacaaatgtg aagaatgtgg
     1981 caaagccttt acaaggtcca caacactgaa tgaacacaag aaaattcata ctggagagaa
     2041 accctacaaa tgtaaagaat gtggcaaagc ctttagatgg tccacaagcc tgaatgaaca
     2101 taagaatatt catactggag agaaacccta caaatgtaaa gaatgtggca aagcctttag
     2161 acagtccagg agcctgaatg aacataaaaa tattcatact ggcgaaaaac cctacacatg
     2221 tgaaaaatgt ggcaaagctt ttaaccaatc ctcaagtctt attatacaca ggagcattca
     2281 ttctgaacaa aaactttaca aatgtgaaga atgtggcaaa gcctttactt ggtcctcatc
     2341 ccttaataaa cataagagaa ttcatactgg agagaaaccc tacacatgtg aagaatgtgg
     2401 caaagctttt tataggtcct cacaccttgc taaacataag agaattcata ctggagagaa
     2461 accctacacg tgcgaagaat gtggcaaagc ttttaaccaa tcctcaactc ttatattaca
     2521 caagagaatc cattctgggc aaaaacctta caaatgtgaa gaatgtggca aagcctttac
     2581 acggtccaca acactgaacg aacataagaa aattcatact ggcgagaaac cctacaaatg
     2641 tgaagaatgt ggcaaagctt tcatatggtc cgcaagcctg aatgaacata agaatattca
     2701 tactggagag aaaccctaca aatgtaaaga atgtggcaaa gcttttaacc aatcctcagg
     2761 ccttattata cacaggagca ttcattctga acaaaaactt tacaaatgtg aagaatgtgg
     2821 caaagccttt actcggtcca cagccctgaa tgaacataag aaaattcatt ctggagagaa
     2881 accctacaaa tgcaaagaat gtggcaaagc ctataactta tcctcaaccc ttactaaaca
     2941 taagagaatt catactggag agaaaccctt cacatgtgaa gaatgtggca aagccttcaa
     3001 ttggtcctca tcccttacta aacataagat aattcatact ggagagaaat cctacaaatg
     3061 tgaagaatgt ggcaaagctt ttaatcggcc ctcaaccctt actgtacaca agcgaattca
     3121 tactggcaag gaacatagtt agtaatgaac tagtccgtaa cttgaaagta tttcgatttc
     3181 ttggctttat atatcttgtg gaaaggacga aatctcggtt aagggtatat aggaacgcgt
     3241 taagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     3301 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     3361 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     3421 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     3481 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     3541 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     3601 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     3661 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     3721 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     3781 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcgt
     3841 cgactttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     3901 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
     3961 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     4021 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     4081 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     4141 gcagtacgta tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     4201 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     4261 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     4321 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     4381 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     4441 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     4501 aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg
     4561 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     4621 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     4681 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
     4741 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     4801 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     4861 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     4921 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     4981 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     5041 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     5101 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     5161 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     5221 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     5281 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     5341 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     5401 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     5461 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     5521 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     5581 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     5641 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     5701 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     5761 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     5821 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     5881 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     5941 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     6001 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     6061 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     6121 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     6181 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     6241 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     6301 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     6361 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     6421 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     6481 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     6541 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     6601 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     6661 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     6721 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     6781 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     6841 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     6901 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     6961 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     7021 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     7081 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     7141 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     7201 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     7261 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     7321 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
     7381 tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac
     7441 gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga
     7501 agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg
     7561 acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat
     7621 aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     7681 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     7741 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     7801 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
     7861 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
     7921 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
     7981 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
     8041 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
     8101 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
     8161 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
     8221 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
     8281 gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg
     8341 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
     8401 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
     8461 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
     8521 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
     8581 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
     8641 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
     8701 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
     8761 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
     8821 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
     8881 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
     8941 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
     9001 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
     9061 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccatgc attgcatctc
     9121 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     9181 agcatgcgtc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     9241 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     9301 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     9361 ggaggcctag gcttttgcaa aaagctttct agaggtacca ccggagctta ccatgaccga
     9421 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
     9481 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
     9541 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     9601 ggtgtgggtc gcggacgacg gcgccgccgt ggcggtctgg accacgccgg agagcgtcga
     9661 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     9721 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     9781 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     9841 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     9901 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     9961 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcca gttaacgaat
    10021 tctagatctt gagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
    10081 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
    10141 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
    10201 atccactttg gcgccggctc gaggcctgca ggtgcaaaga tggataaagt tttaaacaga
    10261 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa tt
//
