LOCUS       sequence-414443-       12282 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses presynaptic low affinity ratiometric ATP sensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12282)
  AUTHORS   Marvin JS, Kokotos AC, Kumar M, Pulido C, Tkachuk AN, Yao JS, Brown 
            TA, Ryan TA
  TITLE     iATPSnFR2: A high-dynamic-range fluorescent sensor for monitoring 
            intracellular ATP.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 May 21;121(21):e2314604121. doi: 
            10.1073/pnas.2314604121. Epub 2024 May 15.
  PUBMED    38748581
REFERENCE   2  (bases 1 to 12282)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-05-21"; volume: "121(21):e2314604121. 
            doi"; pages: " 10.1073/pnas.2314604121. Epub 2024 May 15"
COMMENT     Sequence Label: plenti.CamKII.(synapto).iATPSnFR2.A95A.A119L.H...
FEATURES             Location/Qualifiers
     source          1..12282
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2602..3890
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     regulatory      3895..3904
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     5421..5442
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5545..5566)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             6070..6951
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEIS"
     misc_feature    6974..7562
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7027..7047)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7445..7456)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7565..7581)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7566..7582)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8087..8267
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8293..8310)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8299..8523
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8569..8997
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8656..8675)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8866..8887
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9006..9026)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9011..9340
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9191..9326
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9253..9272
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9388..9435
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9454..9828
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9958..10079
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9995..10014)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10049..10068
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10128..10144)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10128..10144)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10141..10163)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10152..10168
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10176..10182,10183..10200,10201..10206))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10221..10242
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10359..10376)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10530..11118)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10610..10629)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11289..12080,12081..12149))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11912..11931
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12150..12254)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta acattatggc cttaggtcac ttcatctcca tggggttctt
     2641 cttctgattt tctagaaaat gagatggggg tgcagagagc ttcctcagtg acctgcccag
     2701 ggtcacatca gaaatgtcag agctagaact tgaactcaga ttactaatct taaattccat
     2761 gccttggggg catgcaagta cgatatacag aaggagtgaa ctcattaggg cagatgacca
     2821 atgagtttag gaaagaagag tccagggcag ggtacatcta caccacccgc ccagccctgg
     2881 gtgagtccag ccacgttcac ctcattatag ttgcctctct ccagtcctac cttgacggga
     2941 agcacaagca gaaactggga caggagcccc aggagaccaa atcttcatgg tccctctggg
     3001 aggatgggtg gggagagctg tggcagaggc ctcaggaggg gccctgctgc tcagtggtga
     3061 cagatagggg tgagaaagca gacagagtca ttccgtcagc attctgggtc tgtttggtac
     3121 ttcttctcac gctaaggtgg cggtgtgata tgcacaatgg ctaaaaagca gggagagctg
     3181 gaaagaaaca aggacagaga cagaggccaa gtcaaccaga ccaattccca gaggaagcaa
     3241 agaaaccatt acagagacta caagggggaa gggaaggaga gatgaattag cttcccctgt
     3301 aaaccttaga acccagctgt tgccagggca acggggcaat acctgtctct tcagaggaga
     3361 tgaagttgcc agggtaacta catcctgtct ttctcaagga ccatcccaga atgtggcacc
     3421 cactagccgt taccatagca actgcctctt tgccccactt aatcccatcc cgtctgttaa
     3481 aagggcccta tagttggagg tgggggaggt aggaagagcg atgatcactt gtggactaag
     3541 tttgttcgca tccccttctc caaccccctc agtacatcac cctgggggaa cagggtccac
     3601 ttgctcctgg gcccacacag tcctgcagta ttgtgtatat aaggccaggg caaagaggag
     3661 caggttttaa agtgaaaggc aggcaggtgt tggggaggca gttaccgggg caacgggaac
     3721 agggcgtttc ggaggtggtt gccatgggga cctggatgct gacgaaggct cgcgaggctg
     3781 tgagcagcca cagtgccctg ctcagaagcc ccaagctcgt cagtcaagcc ggttctccgt
     3841 ttgcactcag gagcacgggc aggcgagtgg cccctagttc tgggggcagc gggggccacc
     3901 atggacgtgg tgaatcagct ggtggctggg ggtcagttcc gggtggtcaa ggagcccctt
     3961 ggcttcgtga aggtgctgca gtgggtcttt gccatcttcg cctttgctac gtgtggcagc
     4021 tacaccgggg agcttcggct gagcgtggag tgtgccaaca agacggagag tgccctcaac
     4081 atcgaagttg aattcgagta ccccttcagg ctgcaccaag tgtactttga tgcaccctcc
     4141 tgcgtcaaag ggggcactac caagatcttc ctggttgggg actactcctc gtcggctgaa
     4201 ttctttgtca ccgtggctgt gtttgccttc ctctactcca tgggggccct ggccacctac
     4261 atcttcctgc agaacaagta ccgagagaac aacaaagggc ctatgatgga ctttctggct
     4321 acagccgtgt tcgctttcat gtggctagtt agttcatcag cctgggccaa aggcctgtcc
     4381 gatgtgaaga tggccacgga cccagagaac attatcaagg agatgcccat gtgccgccag
     4441 acagggaaca catgcaagga actgagggac cctgtgactt caggactcaa cacctcagtg
     4501 gtgtttggct tcctgaacct ggtgctctgg gttggcaact tatggttcgt gttcaaggag
     4561 acaggctggg cagccccatt catgcgcgca cctccaggcg ccccggaaaa gcaaccagca
     4621 cctggcgatg cctacggcga tgcgggctac gggcagggcc ccggaggcta tgggccccag
     4681 gactcctacg ggcctcaggg tggttatcaa cccgattacg ggcagccagc cagcggtggc
     4741 ggtggctacg ggcctcaggg cgactatggg cagcaaggct atggccaaca gggtgcgccc
     4801 acctccttct ccaatcagat gggatccact agtaacggcc gccagtgtgc tggaattctg
     4861 cagatatcga tgggcagatc tatgaagact attcacgtga gtgtcgtaac tcccgacggg
     4921 cctgtatatg aggatgacgt tgaaatggtg agcgtcaaag caaaaagtgg cgagctcggt
     4981 attctcccag gccacattcc cttgaaagct cccctagaga tcagtgccgc acgcctgaag
     5041 aaagggggca aaacacagta tatcgctgtg tcaggcggca acctcgaagt gcggcctgac
     5101 aaggtgacca tctatgccca ggcagccgag agggctgagg atatcgatgt cctgcgcgcc
     5161 aaggccgcca aggagagagc cgagcgccga ctccaatcac aggtcctggt cgggaacgtc
     5221 tatatcaccg ccgacaagca gaagaacggc atcaaggcga acttcaagat ccgccacaac
     5281 gtggaggacg gcagcatgca gctcgccgac cactaccagc agaacacccc catcggcgac
     5341 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgtgct gagcaaagac
     5401 cctaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     5461 ctcggcatgg acgagctgta caagggcggt accggaggga gcatgagcaa gggcgaggag
     5521 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     5581 ttcagcgtgc gcggcgaggg cgagggcgat gccaccaacg gcaagctgac cctgaagttc
     5641 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
     5701 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     5761 gccatgcccg aaggctacgt ccaggagcgc accatcagct tcaaggacga cggcacctac
     5821 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     5881 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactttatt
     5941 tgtgtggaca tcgattttaa gcgcgccgag ctcttgctta agcgcgcaat gaataggctc
     6001 tcagttgccg aaatgaagct gcagtctacc ggctccggaa atgctgtggg ccaggacacg
     6061 caggagcgtg aaatcggcac aggcttccct ttcgaccctc actacgtgga agtgctgggc
     6121 gagagaatgc actatgtgga cgtgggcccc agagatggaa cccctgtgct gtttctgcac
     6181 ggcaacccta ccagctctta cgtgtggcgg aacatcatcc ctcacgtggc ccctacacac
     6241 agatgtatcg cccctgacct gatcggcatg ggcaagtctg acaagcctga cctgggctac
     6301 ttcttcgacg accacgtgcg gttcatggac gccttcatcg aagccctcgg actggaagag
     6361 gtggtgctgg tcatccacga ttggggatct gccctgggat tccactgggc caagaggaac
     6421 cctgagagag tgaagggaat cgccttcatg gagttcatca ggcccattcc tacctgggac
     6481 gagtggcctg agttcgccag agagacattc caggccttca ggaccacaga cgtgggcaga
     6541 aagctgatca tcgaccagaa cgtgttcatc gagggcaccc tgcctatggg cgttgtcaga
     6601 ccactgaccg aggtggaaat ggaccactac agagagccct ttctgaaccc cgtggacagg
     6661 gaacctctgt ggcggtttcc taacgagctg cctatcgctg gcgagcccgc taatatcgtg
     6721 gctctggtgg aagagtacat ggactggctg catcagagcc ccgtgcctaa gctgctgttc
     6781 tggggaacac ctggcgtgct gatccctcct gctgaagctg ctagactggc caagagcctg
     6841 cctaactgca aggccgtgga tatcggccct ggactgaacc tgctgcaaga ggacaacccc
     6901 gatctgatcg gctccgagat cgccagatgg ctgagcaccc tggaaatctc ttagcgatat
     6961 caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     7021 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     7081 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     7141 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     7201 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     7261 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     7321 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     7381 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     7441 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     7501 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     7561 gcatcgatac cgtcgacctc gagacctaga aaaacatgga gcaatcacaa gtagcaatac
     7621 agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt
     7681 tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     7741 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     7801 tatccttgat ctgtggatct accacacaca aggctacttc cctgattggc agaactacac
     7861 accagggcca gggatcagat atccactgac ctttggatgg tgctacaagc tagtaccagt
     7921 tgagcaagag aaggtagaag aagccaatga aggagagaac acccgcttgt tacaccctgt
     7981 gagcctgcat gggatggatg acccggagag agaagtatta gagtggaggt ttgacagccg
     8041 cctagcattt catcacatgg cccgagagct gcatccggac tgtactgggt ctctctggtt
     8101 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     8161 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     8221 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa
     8281 cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     8341 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     8401 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     8461 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
     8521 tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta
     8581 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     8641 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     8701 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     8761 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     8821 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     8881 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     8941 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat
     9001 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     9061 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     9121 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     9181 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     9241 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa
     9301 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat
     9361 atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc
     9421 atagtataat acgacaaggt gaggaactaa accatggcca agttgaccag tgccgttccg
     9481 gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc
     9541 tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc
     9601 atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc
     9661 ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc
     9721 tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc
     9781 gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca cgtgctacga
     9841 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     9901 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
     9961 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
    10021 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
    10081 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
    10141 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
    10201 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
    10261 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
    10321 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
    10381 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
    10441 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
    10501 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
    10561 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
    10621 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
    10681 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
    10741 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
    10801 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
    10861 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
    10921 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
    10981 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
    11041 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
    11101 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
    11161 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
    11221 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
    11281 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
    11341 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
    11401 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
    11461 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
    11521 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
    11581 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
    11641 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
    11701 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
    11761 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
    11821 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
    11881 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
    11941 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
    12001 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
    12061 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
    12121 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
    12181 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
    12241 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg ac
//
