LOCUS       sequence-414452-       11317 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses cytosolic medium affinity ratiometric ATP sensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11317)
  AUTHORS   Marvin JS, Kokotos AC, Kumar M, Pulido C, Tkachuk AN, Yao JS, Brown 
            TA, Ryan TA
  TITLE     iATPSnFR2: A high-dynamic-range fluorescent sensor for monitoring 
            intracellular ATP.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 May 21;121(21):e2314604121. doi: 
            10.1073/pnas.2314604121. Epub 2024 May 15.
  PUBMED    38748581
REFERENCE   2  (bases 1 to 11317)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-05-21"; volume: "121(21):e2314604121. 
            doi"; pages: " 10.1073/pnas.2314604121. Epub 2024 May 15"
COMMENT     Sequence Label: plenti.CamKII.(cyto).iATPSnFR2.A95K.HaloTag
FEATURES             Location/Qualifiers
     source          1..11317
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(58..77)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        249..628
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        630..828
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     783..803
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             846..1026
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1073..1198
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1691..1924
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2109..2153
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2451..2568
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2613..3901
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     primer_bind     4456..4477
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4580..4601)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    6020..6608
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6073..6093)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6491..6502)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6611..6627)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6612..6628)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7133..7313
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7339..7356)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7345..7569
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7615..8043
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7702..7721)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7912..7933
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8052..8072)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8057..8386
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8237..8372
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8299..8318
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8434..8481
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8500..8874
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9004..9125
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9041..9060)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9095..9114
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9174..9190)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9174..9190)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9187..9209)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9198..9214
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9222..9228,9229..9246,9247..9252))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9267..9288
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9405..9422)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9576..10164)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9656..9675)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10335..11126,11127..11195))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10958..10977
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11196..11300)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac
       61 aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt
      121 cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg
      181 catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata
      241 tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
      301 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
      361 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
      421 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
      481 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
      541 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
      601 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
      661 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
      721 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
      781 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagcg cgttttgcct
      841 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
      901 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
      961 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     1021 ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg
     1081 acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag
     1141 tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag
     1201 tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa
     1261 gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt
     1321 taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc
     1381 atcccttcag acaggatcag aagaacttag atcattatat aatacagtag caaccctcta
     1441 ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga
     1501 agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag
     1561 gaggagatat gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg
     1621 aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag
     1681 cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg
     1741 cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc
     1801 agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg
     1861 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
     1921 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
     1981 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
     2041 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
     2101 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
     2161 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
     2221 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
     2281 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag
     2341 gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat
     2401 cggcactgcg tgcgccaatt ctgcagacaa atggcagtat tcatccacaa ttttaaaaga
     2461 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     2521 atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac
     2581 agggacagca gagatccagt ttggttaatt aacattatgg ccttaggtca cttcatctcc
     2641 atggggttct tcttctgatt ttctagaaaa tgagatgggg gtgcagagag cttcctcagt
     2701 gacctgccca gggtcacatc agaaatgtca gagctagaac ttgaactcag attactaatc
     2761 ttaaattcca tgccttgggg gcatgcaagt acgatataca gaaggagtga actcattagg
     2821 gcagatgacc aatgagttta ggaaagaaga gtccagggca gggtacatct acaccacccg
     2881 cccagccctg ggtgagtcca gccacgttca cctcattata gttgcctctc tccagtccta
     2941 ccttgacggg aagcacaagc agaaactggg acaggagccc caggagacca aatcttcatg
     3001 gtccctctgg gaggatgggt ggggagagct gtggcagagg cctcaggagg ggccctgctg
     3061 ctcagtggtg acagataggg gtgagaaagc agacagagtc attccgtcag cattctgggt
     3121 ctgtttggta cttcttctca cgctaaggtg gcggtgtgat atgcacaatg gctaaaaagc
     3181 agggagagct ggaaagaaac aaggacagag acagaggcca agtcaaccag accaattccc
     3241 agaggaagca aagaaaccat tacagagact acaaggggga agggaaggag agatgaatta
     3301 gcttcccctg taaaccttag aacccagctg ttgccagggc aacggggcaa tacctgtctc
     3361 ttcagaggag atgaagttgc cagggtaact acatcctgtc tttctcaagg accatcccag
     3421 aatgtggcac ccactagccg ttaccatagc aactgcctct ttgccccact taatcccatc
     3481 ccgtctgtta aaagggccct atagttggag gtgggggagg taggaagagc gatgatcact
     3541 tgtggactaa gtttgttcgc atccccttct ccaaccccct cagtacatca ccctggggga
     3601 acagggtcca cttgctcctg ggcccacaca gtcctgcagt attgtgtata taaggccagg
     3661 gcaaagagga gcaggtttta aagtgaaagg caggcaggtg ttggggaggc agttaccggg
     3721 gcaacgggaa cagggcgttt cggaggtggt tgccatgggg acctggatgc tgacgaaggc
     3781 tcgcgaggct gtgagcagcc acagtgccct gctcagaagc cccaagctcg tcagtcaagc
     3841 cggttctccg tttgcactca ggagcacggg caggcgagtg gcccctagtt ctgggggcag
     3901 cgggggatcc gccaccatga agactattca cgtgagtgtc gtaactcccg acgggcctgt
     3961 atatgaggat gacgttgaaa tggtgagcgt caaagcaaaa agtggcgagc tcggtattct
     4021 cccaggccac attcccttga aagctcccct agagatcagt gccgcacgcc tgaagaaagg
     4081 gggcaaaaca cagtatatcg ctgtgtcagg cggcaacctc gaagtgcggc ctgacaaggt
     4141 gaccatctat gcccaggcag ccgagagggc tgaggatatc gatgtcctgc gcgccaagaa
     4201 agccaaggag agagccgagc gccgactcca atcacaggtc ctggtcggga acgtctatat
     4261 caccgccgac aagcagaaga acggcatcaa ggcgaacttc aagatccgcc acaacgtgga
     4321 ggacggcagc atgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     4381 cgtgctgctg cccgacaacc actacctgag cacccagtcc gtgctgagca aagaccctaa
     4441 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     4501 catggacgag ctgtacaagg gcggtaccgg agggagcatg agcaagggcg aggagctgtt
     4561 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     4621 cgtgcgcggc gagggcgagg gcgatgccac caacggcaag ctgaccctga agttcatctg
     4681 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
     4741 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     4801 gcccgaaggc tacgtccagg agcgcaccat cagcttcaag gacgacggca cctacaagac
     4861 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     4921 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact ttatttgtgt
     4981 ggacatcgat tttaagcgcg ccgagctcgc ccttaagcgc gcaatgaata ggctctcagt
     5041 tgccgaaatg aagctgcagg gcggaggctc gggcggaggt gggtcgggtg gcggcggatc
     5101 aatggccgag atcggcaccg gcttcccctt cgacccccac tacgtggagg tgctgggcga
     5161 gcgcatgcac tacgttgacg tgggcccccg cgacggcacc cccgtgctgt tcctgcacgg
     5221 caaccccacc agcagctacg tgtggcgcaa catcatcccc cacgtggccc ccacccaccg
     5281 cactatcgcc cccgacctga tcggcatggg caagagcgac aagcccgacc tgggctactt
     5341 cttcgacgac cacgtgcgct tcatggacgc cttcatcgag gccctgggcc tggaggaggt
     5401 ggtgctggtg atccacgact ggggcagcgc cctgggcttc cactgggcca agcgcaaccc
     5461 cgagcgcgtg aagggcatcg ccttcatgga gttcatccgc cccatcccca cctgggacga
     5521 gtggcccgag ttcgcccgcg agaccttcca ggccttccgc accaccgacg tgggccgcaa
     5581 gctgatcatc gaccagaacg tgttcatcga gggcaccctg cccatgggcg tggtgcgccc
     5641 cctgaccgag gtggagatgg accactaccg cgagcccttc ctgaaccccg tggaccgcga
     5701 gcccctgtgg cgcttcccca acgagctgcc catcgccggc gagcccgcca acatcgtggc
     5761 cctggtggag gagtacatgg actggctgca ccagagcccc gtgcccaagc tgctgttctg
     5821 gggcaccccc ggcgtgctga tcccccccgc cgaggccgcc cgcctggcca agagcctgcc
     5881 caacgttaag gccgtggaca tcggccccgg cctgaacctg ctccaggagg acaaccccga
     5941 cctgatcggc agcgagatcg cccgctggct gagcaccctc gagatcagcg gctaggaatt
     6001 cgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg
     6061 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     6121 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     6181 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     6241 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     6301 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     6361 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     6421 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     6481 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     6541 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     6601 ctccccgcat cgataccgtc gacctcgaga cctagaaaaa catggagcaa tcacaagtag
     6661 caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt
     6721 gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga
     6781 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     6841 acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg attggcagaa
     6901 ctacacacca gggccaggga tcagatatcc actgaccttt ggatggtgct acaagctagt
     6961 accagttgag caagagaagg tagaagaagc caatgaagga gagaacaccc gcttgttaca
     7021 ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtattagagt ggaggtttga
     7081 cagccgccta gcatttcatc acatggcccg agagctgcat ccggactgta ctgggtctct
     7141 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     7201 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     7261 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagggcccg
     7321 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     7381 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     7441 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     7501 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     7561 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc
     7621 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     7681 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     7741 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     7801 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     7861 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     7921 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     7981 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     8041 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     8101 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     8161 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     8221 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     8281 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt
     8341 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc
     8401 ttgtatatcc attttcggat ctgatcagca cgtgttgaca attaatcatc ggcatagtat
     8461 atcggcatag tataatacga caaggtgagg aactaaacca tggccaagtt gaccagtgcc
     8521 gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac cgaccggctc
     8581 gggttctccc gggacttcgt ggaggacgac ttcgccggtg tggtccggga cgacgtgacc
     8641 ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg
     8701 gtgcgcggcc tggacgagct gtacgccgag tggtcggagg tcgtgtccac gaacttccgg
     8761 gacgcctccg ggccggccat gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc
     8821 ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga ctgacacgtg
     8881 ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     8941 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     9001 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     9061 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     9121 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     9181 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     9241 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     9301 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     9361 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     9421 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     9481 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     9541 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     9601 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     9661 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     9721 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     9781 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     9841 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     9901 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     9961 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
    10021 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
    10081 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
    10141 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
    10201 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
    10261 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
    10321 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
    10381 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
    10441 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
    10501 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
    10561 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
    10621 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
    10681 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
    10741 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
    10801 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
    10861 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
    10921 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
    10981 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
    11041 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
    11101 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
    11161 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
    11221 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
    11281 aaacaaatag gggttccgcg cacatttccc cgaaaag
//
