LOCUS       sequence-414453-       10867 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses cytosolic low affinity ratiometric ATP sensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10867)
  AUTHORS   Marvin JS, Kokotos AC, Kumar M, Pulido C, Tkachuk AN, Yao JS, Brown 
            TA, Ryan TA
  TITLE     iATPSnFR2: A high-dynamic-range fluorescent sensor for monitoring 
            intracellular ATP.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 May 21;121(21):e2314604121. doi: 
            10.1073/pnas.2314604121. Epub 2024 May 15.
  PUBMED    38748581
REFERENCE   2  (bases 1 to 10867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-05-21"; volume: "121(21):e2314604121. 
            doi"; pages: " 10.1073/pnas.2314604121. Epub 2024 May 15"
COMMENT     Sequence Label: 
            plenti.CamKII.(cyto).iATPSnFR2.A95A.A119L.miRFP670nano3
FEATURES             Location/Qualifiers
     source          1..10867
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(28..47)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        219..598
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        600..798
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     753..773
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             816..996
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1043..1168
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1661..1894
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2079..2123
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2421..2538
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2583..3871
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     primer_bind     4426..4447
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4550..4571)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    5540..6128
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5593..5613)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6011..6022)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6131..6147)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6132..6148)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6653..6833
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6859..6876)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6865..7089
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7135..7563
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7222..7241)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7432..7453
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7572..7592)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7577..7906
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7757..7892
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7819..7838
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7954..8001
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8020..8394
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8524..8645
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8561..8580)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8615..8634
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8694..8710)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8694..8710)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8707..8729)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8718..8734
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8742..8748,8749..8766,8767..8772))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8787..8808
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8925..8942)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9096..9684)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9176..9195)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9855..10646,10647..10715))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10478..10497
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10716..10820)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
       61 gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc
      121 tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt
      181 tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta
      241 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
      301 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
      361 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
      421 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
      481 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
      541 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
      601 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
      661 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
      721 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
      781 ggaggtctat ataagcagcg cgttttgcct gtactgggtc tctctggtta gaccagatct
      841 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
      901 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
      961 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
     1021 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
     1081 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     1141 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     1201 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     1261 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     1321 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     1381 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     1441 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     1501 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     1561 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     1621 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     1681 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     1741 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     1801 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     1861 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     1921 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     1981 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     2041 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     2101 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     2161 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     2221 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     2281 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
     2341 gagacagatc cattcgatta gtgaacggat cggcactgcg tgcgccaatt ctgcagacaa
     2401 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
     2461 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     2521 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt ttggttaatt
     2581 aacattatgg ccttaggtca cttcatctcc atggggttct tcttctgatt ttctagaaaa
     2641 tgagatgggg gtgcagagag cttcctcagt gacctgccca gggtcacatc agaaatgtca
     2701 gagctagaac ttgaactcag attactaatc ttaaattcca tgccttgggg gcatgcaagt
     2761 acgatataca gaaggagtga actcattagg gcagatgacc aatgagttta ggaaagaaga
     2821 gtccagggca gggtacatct acaccacccg cccagccctg ggtgagtcca gccacgttca
     2881 cctcattata gttgcctctc tccagtccta ccttgacggg aagcacaagc agaaactggg
     2941 acaggagccc caggagacca aatcttcatg gtccctctgg gaggatgggt ggggagagct
     3001 gtggcagagg cctcaggagg ggccctgctg ctcagtggtg acagataggg gtgagaaagc
     3061 agacagagtc attccgtcag cattctgggt ctgtttggta cttcttctca cgctaaggtg
     3121 gcggtgtgat atgcacaatg gctaaaaagc agggagagct ggaaagaaac aaggacagag
     3181 acagaggcca agtcaaccag accaattccc agaggaagca aagaaaccat tacagagact
     3241 acaaggggga agggaaggag agatgaatta gcttcccctg taaaccttag aacccagctg
     3301 ttgccagggc aacggggcaa tacctgtctc ttcagaggag atgaagttgc cagggtaact
     3361 acatcctgtc tttctcaagg accatcccag aatgtggcac ccactagccg ttaccatagc
     3421 aactgcctct ttgccccact taatcccatc ccgtctgtta aaagggccct atagttggag
     3481 gtgggggagg taggaagagc gatgatcact tgtggactaa gtttgttcgc atccccttct
     3541 ccaaccccct cagtacatca ccctggggga acagggtcca cttgctcctg ggcccacaca
     3601 gtcctgcagt attgtgtata taaggccagg gcaaagagga gcaggtttta aagtgaaagg
     3661 caggcaggtg ttggggaggc agttaccggg gcaacgggaa cagggcgttt cggaggtggt
     3721 tgccatgggg acctggatgc tgacgaaggc tcgcgaggct gtgagcagcc acagtgccct
     3781 gctcagaagc cccaagctcg tcagtcaagc cggttctccg tttgcactca ggagcacggg
     3841 caggcgagtg gcccctagtt ctgggggcag cgggggatcc gccaccatga agactattca
     3901 cgtgagtgtc gtaactcccg acgggcctgt atatgaggat gacgttgaaa tggtgagcgt
     3961 caaagcaaaa agtggcgagc tcggtattct cccaggccac attcccttga aagctcccct
     4021 agagatcagt gccgcacgcc tgaagaaagg gggcaaaaca cagtatatcg ctgtgtcagg
     4081 cggcaacctc gaagtgcggc ctgacaaggt gaccatctat gcccaggcag ccgagagggc
     4141 tgaggatatc gatgtcctgc gcgccaaggc cgccaaggag agagccgagc gccgactcca
     4201 atcacaggtc ctggtcggga acgtctatat caccgccgac aagcagaaga acggcatcaa
     4261 ggcgaacttc aagatccgcc acaacgtgga ggacggcagc atgcagctcg ccgaccacta
     4321 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     4381 cacccagtcc gtgctgagca aagaccctaa cgagaagcgc gatcacatgg tcctgctgga
     4441 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagg gcggtaccgg
     4501 agggagcatg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     4561 ggacggcgac gtaaacggcc acaagttcag cgtgcgcggc gagggcgagg gcgatgccac
     4621 caacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     4681 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
     4741 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     4801 cagcttcaag gacgacggca cctacaagac ccgcgccgag gtgaagttcg agggcgacac
     4861 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     4921 gcacaagctg gagtacaact ttatttgtgt ggacatcgat tttaagcgcg ccgagctctt
     4981 gcttaagcgc gcaatgaata ggctctcagt tgccgaaatg aagctgcagg gcggaggctc
     5041 gggcggaggt gggtcgggtg gcggcggatc aatggcaaac ctggacaaga tgctgaacac
     5101 caccgtgacc gaggtgcgca agttcctgca agcggacaga gtgtgcgtgt tcaagttcga
     5161 ggaagattac tccggcaccg tctcgcacga agccgtggac gacagatgga ttagcatcct
     5221 gaagacccag gtgcaggaca gatacttcat ggaaaccaga ggcgaggaat acgtccacgg
     5281 cagataccag gccatcgccg acatctacac agccaatctg gtcgagtgct acagagacct
     5341 gctgatcgag tttcaggtgc gggccattct ggctgtcccc atcctgcaag gcaagaagct
     5401 gtggggcctg ctggtggccc accaactggc cggccctcgg gagtggcaga cctgggaaat
     5461 cgacttcctg aaacagcaag ccgtggtgat gggcatcgcc atccagcaga gctaggaatt
     5521 cgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg
     5581 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     5641 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     5701 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     5761 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     5821 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     5881 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     5941 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     6001 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     6061 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     6121 ctccccgcat cgataccgtc gacctcgaga cctagaaaaa catggagcaa tcacaagtag
     6181 caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt
     6241 gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga
     6301 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     6361 acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg attggcagaa
     6421 ctacacacca gggccaggga tcagatatcc actgaccttt ggatggtgct acaagctagt
     6481 accagttgag caagagaagg tagaagaagc caatgaagga gagaacaccc gcttgttaca
     6541 ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtattagagt ggaggtttga
     6601 cagccgccta gcatttcatc acatggcccg agagctgcat ccggactgta ctgggtctct
     6661 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     6721 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     6781 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagggcccg
     6841 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     6901 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     6961 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     7021 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     7081 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc
     7141 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     7201 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     7261 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     7321 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     7381 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     7441 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     7501 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     7561 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     7621 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     7681 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     7741 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     7801 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt
     7861 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc
     7921 ttgtatatcc attttcggat ctgatcagca cgtgttgaca attaatcatc ggcatagtat
     7981 atcggcatag tataatacga caaggtgagg aactaaacca tggccaagtt gaccagtgcc
     8041 gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac cgaccggctc
     8101 gggttctccc gggacttcgt ggaggacgac ttcgccggtg tggtccggga cgacgtgacc
     8161 ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg
     8221 gtgcgcggcc tggacgagct gtacgccgag tggtcggagg tcgtgtccac gaacttccgg
     8281 gacgcctccg ggccggccat gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc
     8341 ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga ctgacacgtg
     8401 ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     8461 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     8521 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     8581 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     8641 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     8701 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     8761 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     8821 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     8881 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     8941 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     9001 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     9061 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     9121 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     9181 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     9241 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     9301 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     9361 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     9421 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     9481 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     9541 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     9601 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     9661 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     9721 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     9781 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     9841 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     9901 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     9961 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
    10021 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
    10081 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
    10141 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
    10201 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
    10261 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
    10321 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
    10381 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
    10441 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
    10501 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
    10561 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
    10621 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
    10681 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
    10741 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
    10801 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg
    10861 ggagatc
//
