LOCUS       sequence-414882-        9965 bp    DNA     circular SYN 15-APR-2025
DEFINITION  doxycycline-inducible expression of Mouse Tbet mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9965)
  AUTHORS   Altman A, Henriques-Oliveira L, Kurochkin I, Ascic E, Halitzki E, 
            Rosa F, Pires C, Pereira C-F
  TITLE     Transcription Factor Blueprints Underlying Dendritic Cell Diversity
  JOURNAL   Exp Hematol. 2024 Aug. 137:104298
REFERENCE   2  (bases 1 to 9965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Exp 
            Hematol. 2024 Aug. 137:104298"
COMMENT     Sequence Label: pFUW-TetO-Tbet
FEATURES             Location/Qualifiers
     source          1..9965
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        27..406
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        408..606
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             624..804
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    851..976
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1469..1702
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1887..1931
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2229..2346
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2403..2421
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2445..2463
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2487..2505
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2529..2547
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2571..2589
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2613..2631
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        2664..2702
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2699..2723
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    4446..5034
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4499..4519)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4917..4928)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5037..5053)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5038..5054)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5559..5739
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5765..5782)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5771..5995
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6041..6469
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6128..6147)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6338..6359
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6478..6498)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6595..6804
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        6616..6812
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6663..6798
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6725..6744
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6860..6907
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6926..7300
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7430..7551
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7467..7486)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7521..7540
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7600..7616)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7600..7616)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7613..7635)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7624..7640
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7648..7654,7655..7672,7673..7678))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7693..7714
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7831..7848)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8002..8590)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8082..8101)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8761..9552,9553..9621))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9384..9403
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9622..9726)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9801..9820)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
       61 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      121 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      181 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      241 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      301 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      361 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
      421 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
      481 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
      541 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      601 aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
      661 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
      721 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
      781 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
      841 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
      901 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
      961 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     1021 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     1081 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     1141 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     1201 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     1261 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     1321 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     1381 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     1441 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     1501 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     1561 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     1621 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     1681 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     1741 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     1801 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     1861 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     1921 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     1981 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     2041 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     2101 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
     2161 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
     2221 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     2281 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     2341 aattttcggg tttattacag ggacagcaga gatccagttt ggttagatct cgagtttacc
     2401 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     2461 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     2521 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
     2581 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
     2641 gtcgagctcg gtacccgggt cgaggtaggc gtgtacggtg ggaggcctat ataagcagag
     2701 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
     2761 gaagacaccg ggaccgatcc agcctccgcg gccccgaatt caccggttct agaatgggca
     2821 tcgtggagcc gggttgcgga gacatgctga cgggcaccga gccgatgccg gggagcgacg
     2881 agggccgggc gcctggcgcc gacccgcagc accgctactt ctacccggag ccgggcgcgc
     2941 aggacgcgga cgagcgtcgc gggggcggca gcctggggtc tccctacccg gggggcgcct
     3001 tggtgcccgc cccgccgagc cgcttccttg gagcctacgc ctacccgccg cgaccccagg
     3061 cggccggctt ccccggcgcg ggcgagtcct tcccgccgcc cgcggacgcc gagggctacc
     3121 agccgggcga gggctacgcc gccccggacc cgcgcgccgg gctctacccg gggccgcgtg
     3181 aggactacgc gctacccgcg ggactggagg tgtcggggaa actgagggtc gcgctcaaca
     3241 accacctgtt gtggtccaag tttaatcagc accagacaga gatgatcatc accaagcagg
     3301 gacggcggat gttcccattc ctgtcattta ctgtggccgg gctggagccc accagccact
     3361 acaggatgtt tgtggacgtg gtcttggtgg accagcacca ctggcggtac cagagcggca
     3421 agtgggtgca gtgtggaaag gccgagggca gcatgccagg aaaccgcctg tacgtccacc
     3481 cggactcccc caacacagga gcgcactgga tgcgccagga agtttcattt gggaaactaa
     3541 agctcacaaa caacaagggg gcgtccaaca atgtgaccca gatgattgtg ctccagtccc
     3601 tccataagta ccagccccgg ctgcatatcg ttgaggtgaa cgacggagag ccagaggcag
     3661 cctgcaacgc ttccaacacg catatcttta ctttccaaga aacccagttc attgccgtga
     3721 ctgcctacca gaatgccgag attactcagc tgaaaattga taataacccc tttgccaaag
     3781 gattccggga gaactttgag tccatgtaca catctgttga caccagcatc ccctccccgc
     3841 ctggacccaa ctgtcaattc cttgggggag atcactactc tcctctccta cccaaccagt
     3901 atcctgttcc cagccgcttc taccccgacc ttcctggcca ggcgaaggat gtggttcccc
     3961 aggcttactg gctgggggcc ccccgggacc acagctatga ggctgagttt cgagcagtca
     4021 gcatgaagcc tgcattcttg ccctctgccc ctgggcccac catgtcctac taccgaggcc
     4081 aggaggtcct ggcacctgga gctggctggc ctgtggcacc ccagtaccct cccaagatgg
     4141 gcccggccag ctggttccgc cctatgcgga ctctgcccat ggaacccggc cctggaggct
     4201 cagagggacg gggaccagag gaccagggtc cccccttggt gtggactgag attgccccca
     4261 tccggccgga atccagtgat tcaggactgg gcgaaggaga ctctaagagg aggcgcgtgt
     4321 ccccctatcc ttccagtggt gacagctcct cccctgctgg ggccccttct ccttttgata
     4381 aggaagctga aggacagttt tataactatt ttcccaacta gttcgaagat atcaagctta
     4441 tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     4501 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     4561 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     4621 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     4681 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     4741 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     4801 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     4861 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     4921 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     4981 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat
     5041 accgtcgacc tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta
     5101 ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca
     5161 cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     5221 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg
     5281 atctgtggat ctaccacaca caaggctact tccctgattg gcagaactac acaccagggc
     5341 cagggatcag atatccactg acctttggat ggtgctacaa gctagtacca gttgagcaag
     5401 agaaggtaga agaagccaat gaaggagaga acacccgctt gttacaccct gtgagcctgc
     5461 atgggatgga tgacccggag agagaagtat tagagtggag gtttgacagc cgcctagcat
     5521 ttcatcacat ggcccgagag ctgcatccgg actgtactgg gtctctctgg ttagaccaga
     5581 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     5641 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     5701 ccctcagacc cttttagtca gtgtggaaaa tctctagcag ggcccgttta aacccgctga
     5761 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     5821 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     5881 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     5941 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     6001 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca
     6061 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     6121 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     6181 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     6241 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     6301 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     6361 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     6421 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga
     6481 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     6541 gcatgcatct caattagtca gcaaccatag kccsrmmsyy mmcwsssycc cyascaggca
     6601 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     6661 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     6721 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
     6781 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
     6841 tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata
     6901 atacgacaag gtgaggaact aaaccatggc caagttgacc agtgccgttc cggtgctcac
     6961 cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga
     7021 cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc
     7081 ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga
     7141 cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc
     7201 ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc
     7261 cggcaactgc gtgcacttcg tggccgagga gcaggactga cacgtgctac gagatttcga
     7321 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
     7381 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
     7441 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     7501 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     7561 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     7621 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     7681 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     7741 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     7801 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     7861 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     7921 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     7981 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     8041 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     8101 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     8161 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     8221 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     8281 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     8341 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     8401 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     8461 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     8521 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     8581 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     8641 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     8701 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     8761 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     8821 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     8881 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     8941 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     9001 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     9061 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     9121 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     9181 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     9241 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     9301 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     9361 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     9421 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     9481 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     9541 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     9601 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     9661 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     9721 tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat
     9781 cccctatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg
     9841 ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca
     9901 aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg
     9961 cttcg
//
