LOCUS       sequence-414885-       11135 bp    DNA     circular SYN 15-APR-2025
DEFINITION  doxycycline-inducible expression of Human NFATC2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11135)
  AUTHORS   Altman A, Henriques-Oliveira L, Kurochkin I, Ascic E, Halitzki E, 
            Rosa F, Pires C, Pereira C-F
  TITLE     Transcription Factor Blueprints Underlying Dendritic Cell Diversity
  JOURNAL   Exp Hematol. 2024 Aug. 137:104298
REFERENCE   2  (bases 1 to 11135)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Exp 
            Hematol. 2024 Aug. 137:104298"
COMMENT     Sequence Label: pFUW-TetO-NFATC2
FEATURES             Location/Qualifiers
     source          1..11135
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        27..406
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        408..606
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             624..804
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    851..976
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1469..1702
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1887..1931
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2229..2346
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2403..2421
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2445..2463
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2487..2505
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2529..2547
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2571..2589
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2613..2631
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        2664..2702
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2699..2723
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      4980..4989
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    5616..6204
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5669..5689)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6087..6098)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6207..6223)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6208..6224)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6729..6909
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6935..6952)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6941..7165
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7211..7639
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7298..7317)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7508..7529
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7648..7668)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7653..7982
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7833..7968
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7895..7914
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8030..8077
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8096..8470
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8600..8721
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8637..8656)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8691..8710
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8770..8786)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8770..8786)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8783..8805)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8794..8810
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8818..8824,8825..8842,8843..8848))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8863..8884
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9001..9018)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9172..9760)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9252..9271)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9931..10722,10723..10791))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10554..10573
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10792..10896)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10971..10990)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
       61 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      121 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      181 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      241 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      301 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      361 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
      421 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
      481 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
      541 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      601 aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
      661 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
      721 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
      781 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
      841 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
      901 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
      961 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     1021 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     1081 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     1141 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     1201 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     1261 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     1321 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     1381 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     1441 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     1501 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     1561 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     1621 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     1681 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     1741 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     1801 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     1861 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     1921 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     1981 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     2041 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     2101 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
     2161 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
     2221 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     2281 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     2341 aattttcggg tttattacag ggacagcaga gatccagttt ggttagatct cgagtttacc
     2401 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     2461 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     2521 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
     2581 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
     2641 gtcgagctcg gtacccgggt cgaggtaggc gtgtacggtg ggaggcctat ataagcagag
     2701 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
     2761 gaagacaccg ggaccgatcc agcctccgcg gccccgaatt caccggttct agaatgaacg
     2821 cccccgagcg gcagccccaa cccgacggcg gggacgcccc aggccacgag cctgggggca
     2881 gcccccaaga cgagcttgac ttctccatcc tcttcgacta tgagtatttg aatccgaacg
     2941 aagaagagcc gaatgcacat aaggtcgcca gcccaccctc cggacccgca taccccgatg
     3001 atgtcctgga ctatggcctc aagccataca gcccccttgc tagtctctct ggcgagcccc
     3061 ccggccgatt cggagagccg gatagggtag ggccgcagaa gtttctgagc gcggccaagc
     3121 cagcaggggc ctcgggcctg agccctcgga tcgagatcac tccgtcccac gaactgatcc
     3181 aggcagtggg gcccctccgc atgagagacg cgggcctcct ggtggagcag ccgcccctgg
     3241 ccggggtggc cgccagcccg aggttcaccc tgcccgtgcc cggcttcgag ggctaccgcg
     3301 agccgctttg cttgagcccc gctagcagcg gctcctctgc cagcttcatt tctgacacct
     3361 tctcccccta cacctcgccc tgcgtctcgc ccaataacgg cgggcccgac gacctgtgtc
     3421 cgcagtttca aaacatccct gctcattatt cccccagaac ctcgccaata atgtcacctc
     3481 gaaccagcct cgccgaggac agctgcctgg gccgccactc gcccgtgccc cgtccggcct
     3541 cccgctcctc atcgcctggt gccaagcgga ggcattcgtg cgccgaggcc ttggttgccc
     3601 tgccgcccgg agcctcaccc cagcgctccc ggagcccctc gccgcagccc tcatctcacg
     3661 tggcacccca ggaccacggc tccccggctg ggtacccccc tgtggctggc tctgccgtga
     3721 tcatggatgc cctgaacagc ctcgccacgg actcgccttg tgggatcccc cccaagatgt
     3781 ggaagaccag ccctgacccc tcgccggtgt ctgccgcccc atccaaggcc ggcctgcctc
     3841 gccacatcta cccggccgtg gagttcctgg ggccctgcga gcagggcgag aggagaaact
     3901 cggctccaga atccatcctg ctggttccgc ccacttggcc caagccgctg gtgcctgcca
     3961 ttcccatctg cagcatccca gtgactgcat ccctccctcc acttgagtgg ccgctgtcca
     4021 gtcagtcagg ctcttacgag ctgcggatcg aggtgcagcc caagccacat caccgggccc
     4081 actatgagac agaaggcagc cgaggggctg tcaaagctcc aactggaggc caccctgtgg
     4141 ttcagctcca tggctacatg gaaaacaagc ctctgggact tcagatcttc attgggacag
     4201 ctgatgagcg gatccttaag ccgcacgcct tctaccaggt gcaccgaatc acggggaaaa
     4261 ctgtcaccac caccagctat gagaagatag tgggcaacac caaagtcctg gagatcccct
     4321 tggagcccaa aaacaacatg agggcaacca tcgactgtgc ggggatcttg aagcttagaa
     4381 acgccgacat tgagctgcgg aaaggcgaga cggacattgg aagaaagaac acgcgggtga
     4441 gactggtttt ccgagttcac atcccagagt ccagtggcag aatcgtctct ttacagactg
     4501 catctaaccc catcgagtgc tcccagcgat ctgctcacga gctgcccatg gttgaaagac
     4561 aagacacaga cagctgcctg gtctatggcg gccagcaaat gatcctcacg gggcagaact
     4621 ttacatccga gtccaaagtt gtgtttactg agaagaccac agatggacag caaatttggg
     4681 agatggaagc cacggtggat aaggacaaga gccagcccaa catgcttttt gttgagatcc
     4741 ctgaatatcg gaacaagcat atccgcacac ctgtaaaagt gaacttctac gtcatcaatg
     4801 ggaagagaaa acgaagtcag cctcagcact ttacctacca cccagtccca gccatcaaga
     4861 cggagcccac ggatgaatat gaccccactc tgatctgcag ccccacccat ggaggcctgg
     4921 ggagccagcc ttactacccc cagcacccga tggtggccga gtccccctcc tgcctcgtgg
     4981 ccaccatggc tccctgccag cagttccgca cggggctctc atcccctgac gcccgctacc
     5041 agcaacagaa cccagcggcc gtactctacc agcggagcaa gagcctgagc cccagcctgc
     5101 tgggctatca gcagccggcc ctcatggccg ccccgctgtc ccttgcggac gctcaccgct
     5161 ctgtgctggt gcacgccggc tcccagggcc agagctcagc cctgctccac ccctctccga
     5221 ccaaccagca ggcctcgcct gtgatccact actcacccac caaccagcag ctgcgctgcg
     5281 gaagccacca ggagttccag cacatcatgt actgcgagaa tttcgcacca ggcaccacca
     5341 gacctggccc gcccccggtc agtcaaggtc agaggctgag cccgggttcc taccccacag
     5401 tcattcagca gcagaatgcc acgagccaaa gagccgccaa aaacggaccc ccggtcagtg
     5461 accaaaagga agtattacct gcgggggtga ccattaaaca ggagcagaac ttggaccaga
     5521 cctacttgga tgatgttaat gaaattatca ggaaggagtt ttcaggacct cctgccagaa
     5581 atcagacgta attcgaagat atcaagctta tcgataatca acctctggat tacaaaattt
     5641 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     5701 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     5761 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     5821 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     5881 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     5941 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     6001 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
     6061 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     6121 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     6181 tctccctttg ggccgcctcc ccgcatcgat accgtcgacc tcgagaccta gaaaaacatg
     6241 gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac
     6301 aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt
     6361 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     6421 ttcactccca acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact
     6481 tccctgattg gcagaactac acaccagggc cagggatcag atatccactg acctttggat
     6541 ggtgctacaa gctagtacca gttgagcaag agaaggtaga agaagccaat gaaggagaga
     6601 acacccgctt gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtat
     6661 tagagtggag gtttgacagc cgcctagcat ttcatcacat ggcccgagag ctgcatccgg
     6721 actgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     6781 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     6841 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     6901 tctctagcag ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
     6961 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     7021 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     7081 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     7141 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg
     7201 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     7261 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     7321 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     7381 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     7441 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     7501 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     7561 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     7621 caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     7681 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     7741 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     7801 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     7861 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     7921 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     7981 aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta
     8041 atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc
     8101 caagttgacc agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt
     8161 ctggaccgac cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt
     8221 ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac
     8281 cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt
     8341 gtccacgaac ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg
     8401 ggggcgggag ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga
     8461 gcaggactga cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     8521 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     8581 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
     8641 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     8701 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
     8761 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     8821 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     8881 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     8941 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     9001 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     9061 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     9121 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     9181 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     9241 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     9301 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     9361 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     9421 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     9481 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     9541 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     9601 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     9661 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     9721 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     9781 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     9841 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     9901 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     9961 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
    10021 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
    10081 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
    10141 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
    10201 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
    10261 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
    10321 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
    10381 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
    10441 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
    10501 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
    10561 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
    10621 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
    10681 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
    10741 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
    10801 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
    10861 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
    10921 tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc
    10981 tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag
    11041 tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag
    11101 aatctgctta gggttaggcg ttttgcgctg cttcg
//
