LOCUS       sequence-414887-       10183 bp    DNA     circular SYN 15-APR-2025
DEFINITION  doxycycline-inducible expression of Human PML in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10183)
  AUTHORS   Altman A, Henriques-Oliveira L, Kurochkin I, Ascic E, Halitzki E, 
            Rosa F, Pires C, Pereira C-F
  TITLE     Transcription Factor Blueprints Underlying Dendritic Cell Diversity
  JOURNAL   Exp Hematol. 2024 Aug. 137:104298
REFERENCE   2  (bases 1 to 10183)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Exp 
            Hematol. 2024 Aug. 137:104298"
COMMENT     Sequence Label: pFUW-TetO-PML
FEATURES             Location/Qualifiers
     source          1..10183
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(20..40)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        25..354
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      205..340
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     267..286
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        402..449
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             468..842
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    972..1093
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1009..1028)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1063..1082
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1142..1158)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1142..1158)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1155..1177)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1166..1182
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1190..1196,1197..1214,1215..1220))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1235..1256
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1373..1390)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1544..2132)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1624..1643)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2303..3094,3095..3163))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2926..2945
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3164..3268)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3343..3362)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3534..3913
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3915..4113
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             4131..4311
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4358..4483
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4976..5209
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5394..5438
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5736..5853
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(5910..5928,5929..5951,5952..5970,5971..5993,5994..6012,
                     6013..6035,6036..6054,6055..6077,6078..6096,6097..6119,
                     6120..6138,6139..6161,6162..6180)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        6213..6251
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6248..6272
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    8171..8759
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8224..8244)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8642..8653)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8762..8778)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8763..8779)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9284..9464
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9490..9507)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9496..9720
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(9766..10183,1..11)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9853..9872)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10063..10084
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
       61 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
      121 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
      181 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
      241 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
      301 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
      361 ccgggagctt gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg
      421 catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagttga
      481 ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg
      541 accggctcgg gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg
      601 acgtgaccct gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct
      661 gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga
      721 acttccggga cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg
      781 agttcgccct gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact
      841 gacacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
      901 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
      961 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     1021 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     1081 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
     1141 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     1201 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     1261 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     1321 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     1381 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     1441 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     1501 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     1561 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     1621 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     1681 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     1741 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     1801 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     1861 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     1921 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     1981 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     2041 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     2101 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     2161 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     2221 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     2281 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     2341 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     2401 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     2461 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     2521 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     2581 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     2641 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     2701 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     2761 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     2821 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     2881 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     2941 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     3001 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     3061 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     3121 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     3181 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     3241 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
     3301 acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc
     3361 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
     3421 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
     3481 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
     3541 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
     3601 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     3661 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     3721 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     3781 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     3841 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     3901 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     3961 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     4021 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     4081 taggcgtgta cggtgggagg tctatataag cagcgcgttt tgcctgtact gggtctctct
     4141 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     4201 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     4261 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
     4321 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
     4381 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
     4441 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
     4501 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
     4561 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
     4621 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
     4681 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
     4741 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
     4801 taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg
     4861 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     4921 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     4981 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     5041 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     5101 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     5161 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     5221 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     5281 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     5341 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     5401 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     5461 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     5521 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     5581 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
     5641 gtggagagag agacagagac agatccattc gattagtgaa cggatcggca ctgcgtgcgc
     5701 caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
     5761 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     5821 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
     5881 ccagtttggt tagatctcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
     5941 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
     6001 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
     6061 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
     6121 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     6181 aaagtgaaag tcgagctcgg tacccgggtc gaggtaggcg tgtacggtgg gaggcctata
     6241 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
     6301 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccccgaattc accggttcta
     6361 gaatggagcc tgcacccgcc cgatctccga ggccccagca ggaccccgcc cggccccagg
     6421 agcccaccat gcctcccccc gagaccccct ctgaaggccg ccagcccagc cccagcccca
     6481 gccctacaga gcgagccccc gcttcggagg aggagttcca gtttctgcgc tgccagcaat
     6541 gccaggcgga agccaagtgc ccgaagctgc tgccttgtct gcacacgctg tgctcaggat
     6601 gcctggaggc gtcgggcatg cagtgcccca tctgccaggc gccctggccc ctaggtgcag
     6661 acacacccgc cctggataac gtctttttcg agagtctgca gcggcgcctg tcggtgtacc
     6721 ggcagattgt ggatgcgcag gctgtgtgca cccgctgcaa agagtcggcc gacttctggt
     6781 gctttgagtg cgagcagctc ctctgcgcca agtgcttcga ggcacaccag tggttcctca
     6841 agcacgaggc ccggccccta gcagagctgc gcaaccagtc ggtgcgtgag ttcctggacg
     6901 gcacccgcaa gaccaacaac atcttctgct ccaaccccaa ccaccgcacc cctacgctga
     6961 ccagcatcta ctgccgagga tgttccaagc cgctgtgctg ctcgtgcgcg ctccttgaca
     7021 gcagccacag tgatctcaag tgcgacatca gcgcagagat ccagcagcga caggaggagc
     7081 tggacgccat gacgcaggcg ctgcaggagc aggatagtgc ctttggcgcg gttcacgcgc
     7141 agatgcacgc ggctgtcggc cagctgggcc gcgcgcgtgc cgagaccgag gagctgatcc
     7201 gcgagcgcgt gcgccaggtg gtagctcacg tgcgggctca ggagcgcgag ctgctggagg
     7261 ctgtggacgc gcggtaccag cgcgactacg aggagatggc cagtcggctg ggccgcctgg
     7321 atgctgtgct gcagcgcatc cgcacgggca gcgcgctggt gcagaggatg aagtgctacg
     7381 cctcggacca ggaggtgctg gacatgcacg gtttcctgcg ccaggcgctc tgccgcctgc
     7441 gccaggagga gccccagagc ctgcaagctg ccgtgcgcac cgatggcttc gacgagttca
     7501 aggtgcgcct gcaggacctc agctcttgca tcacccaggg gaaagatgca gctgtatcca
     7561 agaaagccag cccagaggct gccagcactc ccagggaccc tattgacgtt gacctggatg
     7621 tctccaatac aacgacagcc cagaagagga agtgcagcca gacccagtgc cccaggaagg
     7681 tcatcaagat ggagtctgag gaggggaagg aggcaaggtt ggctcggagc tccccggagc
     7741 agcccaggcc cagcacctcc aaggcagtct caccacccca cctggatgga ccgcctagcc
     7801 ccaggagccc cgtcatagga agtgaggtct tcctgcccaa cagcaaccac gtggccagtg
     7861 gcgccgggga ggcagaggaa cgcgttgtgg tgatcagcag ctcggaagac tcagatgccg
     7921 aaaactcgtg catggagccc atggagaccg ccgagccaca gtcctcgcca gcccactcct
     7981 cgccagccca ctcctcgcca gcccactcct cgccagtcca gtctctgctg agagcacaag
     8041 gagcctccag cctgccctgt ggcacatacc accccccagc ttggcctccc caccagcccg
     8101 ctgagcaggc tgccaccccc gatgctgagc ctcccagcag agctgattcg aagatatcaa
     8161 gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     8221 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     8281 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     8341 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     8401 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     8461 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     8521 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     8581 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     8641 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     8701 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca
     8761 tcgataccgt cgacctcgag acctagaaaa acatggagca atcacaagta gcaatacagc
     8821 agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc
     8881 agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     8941 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatat
     9001 ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga actacacacc
     9061 agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag taccagttga
     9121 gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac accctgtgag
     9181 cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg acagccgcct
     9241 agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc tctggttaga
     9301 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     9361 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     9421 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc gtttaaaccc
     9481 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     9541 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     9601 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     9661 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     9721 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     9781 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     9841 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     9901 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     9961 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
    10021 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
    10081 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
    10141 tttcggccta ttggttaaaa aatgagctga tttaacaaaa att
//
