LOCUS       sequence-414889-        9722 bp    DNA     circular SYN 15-APR-2025
DEFINITION  doxycycline-inducible expression of Human NFIX in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9722)
  AUTHORS   Altman A, Henriques-Oliveira L, Kurochkin I, Ascic E, Halitzki E, 
            Rosa F, Pires C, Pereira C-F
  TITLE     Transcription Factor Blueprints Underlying Dendritic Cell Diversity
  JOURNAL   Exp Hematol. 2024 Aug. 137:104298
REFERENCE   2  (bases 1 to 9722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Exp 
            Hematol. 2024 Aug. 137:104298"
COMMENT     Sequence Label: pFUW-TetO-NFIX
FEATURES             Location/Qualifiers
     source          1..9722
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    join(13..31,32..54,55..73,74..96,97..115,116..138,139..157,
                     158..180,181..199,200..222,223..241,242..264,265..283)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        316..354
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     351..375
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    1813..2401
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1866..1886)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2284..2295)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2404..2420)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2405..2421)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2926..3106
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3132..3149)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3138..3362
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3408..3836
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3495..3514)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3705..3726
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3845..3865)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3850..4179
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4030..4165
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4092..4111
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4227..4274
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4293..4667
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    4797..4918
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4834..4853)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4888..4907
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4967..4983)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4967..4983)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4980..5002)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4991..5007
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5015..5021,5022..5039,5040..5045))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5060..5081
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5198..5215)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5369..5957)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5449..5468)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6128..6919,6920..6988))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6751..6770
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6989..7093)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7168..7187)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7359..7738
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7740..7938
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             7956..8136
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8183..8308
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8801..9034
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9219..9263
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9561..9678
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
       61 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt
      121 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
      181 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
      241 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagct
      301 cggtacccgg gtcgaggtag gcgtgtacgg tgggaggcct atataagcag agctcgttta
      361 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
      421 cgggaccgat ccagcctccg cggccccgaa ttcaccggtt ctagaatgct cccggcttgc
      481 cgcctgcagg atgagttcca cccgttcatc gaggcactgc tgcctcacgt ccgcgctttc
      541 tcctacacct ggttcaacct gcaggcgcgg aagcgcaagt acttcaagaa gcatgaaaag
      601 cggatgtcga aggacgagga gcgggcggtg aaggacgagc tgctgggcga gaagcccgag
      661 atcaagcaga agtgggcatc ccggctgctg gccaagctgc gcaaggacat ccggcccgag
      721 ttccgcgagg acttcgtgct gaccatcacg ggcaagaagc ccccctgctg cgtgctctcc
      781 aaccccgacc agaagggcaa gatccggcgg attgactgcc tgcgccaggc tgacaaggtg
      841 tggcggctgg acctggtcat ggtgattttg tttaagggga tccccctgga aagtactgat
      901 ggggagcggc tctacaagtc gcctcagtgc tcgaaccccg gcctgtgcgt ccagccacat
      961 cacattggag tcacaatcaa agaactggat ctttatctgg cttactttgt ccacactccg
     1021 gaatccggac aatcagatag ttcaaaccag caaggagatg cggacatcaa accactgccc
     1081 aacgggcact taagtttcca ggactgtttt gtgacttccg gggtctggaa tgtgacggag
     1141 ctggtgagag tatcacagac tcctgttgca acagcatcag ggcccaactt ctccctggcg
     1201 gacctggaga gtcccagcta ctacaacatc aaccaggtga ccctggggcg gcggtccatc
     1261 acctcccctc cttccaccag caccaccaag cgccccaagt ccatcgatga cagtgagatg
     1321 gagagccctg ttgatgacgt gttctatccc gggacaggcc gttccccagc agctggcagc
     1381 agccagtcca gcgggtggcc caacgatgtg gatgcaggcc cggcttctct aaagaagtca
     1441 ggaaagctgg acttctgcag tgccctctcc tctcagggca gctccccgcg catggctttc
     1501 acccaccacc cgctgcctgt gcttgctgga gtcagaccag ggagcccccg ggccacagca
     1561 tcagccctgc acttcccctc cacgtccatc atccagcagt cgagcccgta tttcacgcac
     1621 ccgaccatcc gctaccacca ccaccacggg caggactcac tgaaggagtt tgtgcagttt
     1681 gtgtgctcgg atggctcggg ccaggccacc ggacagcatt cgcaacgaca ggcgcctcct
     1741 ctgccaaccg gtttgtcagc atcggacccc gggacggcaa ctttctgatt cgaagatatc
     1801 aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     1861 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     1921 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     1981 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     2041 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     2101 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     2161 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     2221 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     2281 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     2341 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     2401 catcgatacc gtcgacctcg agacctagaa aaacatggag caatcacaag tagcaataca
     2461 gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt
     2521 ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     2581 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     2641 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca
     2701 ccagggccag ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt
     2761 gagcaagaga aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg
     2821 agcctgcatg ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc
     2881 ctagcatttc atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta
     2941 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     3001 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     3061 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac
     3121 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     3181 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     3241 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     3301 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     3361 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
     3421 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     3481 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     3541 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     3601 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     3661 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     3721 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     3781 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
     3841 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     3901 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     3961 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     4021 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     4081 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
     4141 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
     4201 tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca
     4261 tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg
     4321 tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct
     4381 cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca
     4441 tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg
     4501 gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct
     4561 ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg
     4621 acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag
     4681 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     4741 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
     4801 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     4861 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     4921 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     4981 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     5041 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     5101 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     5161 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     5221 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     5281 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     5341 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     5401 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     5461 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     5521 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     5581 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     5641 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     5701 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     5761 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     5821 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     5881 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     5941 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     6001 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     6061 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     6121 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     6181 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     6241 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     6301 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     6361 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     6421 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     6481 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     6541 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     6601 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     6661 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     6721 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     6781 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     6841 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     6901 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     6961 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     7021 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     7081 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
     7141 tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     7201 gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc
     7261 tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt
     7321 tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta
     7381 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
     7441 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
     7501 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
     7561 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
     7621 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
     7681 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
     7741 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
     7801 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
     7861 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
     7921 ggaggtctat ataagcagcg cgttttgcct gtactgggtc tctctggtta gaccagatct
     7981 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     8041 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     8101 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
     8161 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
     8221 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     8281 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     8341 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     8401 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     8461 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     8521 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     8581 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     8641 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     8701 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     8761 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     8821 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     8881 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     8941 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     9001 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     9061 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     9121 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     9181 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     9241 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     9301 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     9361 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     9421 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
     9481 gagacagatc cattcgatta gtgaacggat cggcactgcg tgcgccaatt ctgcagacaa
     9541 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
     9601 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     9661 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt ttggttagat
     9721 ct
//
