LOCUS       sequence-414894-        9917 bp    DNA     circular SYN 15-APR-2025
DEFINITION  doxycycline-inducible expression of Human IKZF3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9917)
  AUTHORS   Altman A, Henriques-Oliveira L, Kurochkin I, Ascic E, Halitzki E, 
            Rosa F, Pires C, Pereira C-F
  TITLE     Transcription Factor Blueprints Underlying Dendritic Cell Diversity
  JOURNAL   Exp Hematol. 2024 Aug. 137:104298
REFERENCE   2  (bases 1 to 9917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Exp 
            Hematol. 2024 Aug. 137:104298"
COMMENT     Sequence Label: pFUW-TetO-IKZF3
FEATURES             Location/Qualifiers
     source          1..9917
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        27..406
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        408..606
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             624..804
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    851..976
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1469..1702
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1887..1931
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2229..2346
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2403..2421,2422..2444,2445..2463,2464..2486,2487..2505,
                     2506..2528,2529..2547,2548..2570,2571..2589,2590..2612,
                     2613..2631,2632..2654,2655..2673)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2706..2744
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2741..2765
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    4398..4986
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4451..4471)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4869..4880)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4989..5005)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4990..5006)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5511..5691
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5717..5734)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5723..5947
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5993..6421
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6080..6099)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6290..6311
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6430..6450)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6435..6764
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6615..6750
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6677..6696
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6812..6859
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6878..7252
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7382..7503
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7419..7438)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7473..7492
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7552..7568)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7552..7568)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7565..7587)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7576..7592
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7600..7606,7607..7624,7625..7630))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7645..7666
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7783..7800)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7954..8542)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8034..8053)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8713..9504,9505..9573))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9336..9355
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9574..9678)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9753..9772)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
       61 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      121 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      181 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      241 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      301 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      361 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
      421 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
      481 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
      541 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      601 aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
      661 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
      721 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
      781 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
      841 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
      901 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
      961 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     1021 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     1081 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     1141 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     1201 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     1261 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     1321 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     1381 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     1441 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     1501 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     1561 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     1621 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     1681 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     1741 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     1801 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     1861 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     1921 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     1981 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     2041 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     2101 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
     2161 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
     2221 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     2281 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     2341 aattttcggg tttattacag ggacagcaga gatccagttt ggttagatct cgagtttacc
     2401 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     2461 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     2521 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
     2581 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
     2641 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagct cggtacccgg
     2701 gtcgaggtag gcgtgtacgg tgggaggcct atataagcag agctcgttta gtgaaccgtc
     2761 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
     2821 ccagcctccg cggccccgaa ttcatggaag atatacaaac aaatgcggaa ctgaaaagca
     2881 ctcaggagca gtctgtgccc gcagaaagtg cagcggtttt gaatgactac agtttaacca
     2941 aatctcatga aatggaaaat gtggacagtg gagaaggccc agccaatgaa gatgaagaca
     3001 taggagatga ttcaatgaaa gtgaaagatg aatacagtga aagagatgag aatgttttaa
     3061 agtcagaacc catgggaaat gcagaagagc ctgaaatccc ttacagctat tcaagagaat
     3121 ataatgaata tgaaaacatt aagttggaga gacatgttgt ctcattcgat agtagcaggc
     3181 caaccagtgg aaagatgaac tgcgatgtgt gtggattatc ctgcatcagc ttcaatgtct
     3241 taatggttca taagcgaagc catactggtg aacgcccatt ccagtgtaat cagtgtgggg
     3301 catcttttac tcagaaaggt aacctcctcc gccacattaa actgcacaca ggggaaaaac
     3361 cttttaagtg tcacctctgc aactatgcat gccaaagaag agatgcgctc acggggcatc
     3421 ttaggacaca ttctgtggag aaaccctaca aatgtgagtt ttgtggaagg agttacaagc
     3481 agagaagttc ccttgaggag cacaaggagc gctgccgtac atttcttcag agcactgacc
     3541 caggggacac tgcaagtgcg gaggcaagac acatcaaagc agagatggga agtgaaagag
     3601 ctctcgtact ggacagatta gcaagcaatg tggcaaaacg aaaaagctca atgcctcaga
     3661 aattcattgg tgagaagcgc cactgctttg atgtcaacta taattcaagt tacatgtatg
     3721 agaaagagag tgagctcata cagacccgca tgatggacca agccatcaat aacgccatca
     3781 gctatcttgg cgccgaagcc ctgcgcccct tggtccagac accgcctgct cccacctcgg
     3841 agatggttcc agttatcagc agcatgtatc ccatagccct cacccgggct gagatgtcaa
     3901 acggtgcccc tcaagagctg gaaaagaaaa gcatccacct tccagagaag agcgtgcctt
     3961 ctgagagagg cctctctccc aacaatagtg gccacgactc cacggacact gacagcaacc
     4021 atgaagaacg ccagaatcac atctatcagc aaaatcacat ggtcctgtct cgggcccgca
     4081 atgggatgcc acttctgaag gaggttcccc gctcttacga actcctcaag cccccgccca
     4141 tctgcccaag agactccgtc aaagtgatca acaaggaagg ggaggtgatg gatgtgtatc
     4201 ggtgtgacca ctgccgcgtc ctcttcctgg actatgtgat gttcacgatt cacatgggct
     4261 gccacggctt ccgtgaccct ttcgagtgta acatgtgtgg atatcgaagc catgatcggt
     4321 atgagttctc gtctcacata gccagaggag aacacagagc cctgctgaag tgagaattcg
     4381 atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     4441 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     4501 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     4561 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     4621 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     4681 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     4741 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     4801 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     4861 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     4921 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     4981 ccccgcatcg ataccgtcga cctcgagacc tagaaaaaca tggagcaatc acaagtagca
     5041 atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg
     5101 gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     5161 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     5221 aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat tggcagaact
     5281 acacaccagg gccagggatc agatatccac tgacctttgg atggtgctac aagctagtac
     5341 cagttgagca agagaaggta gaagaagcca atgaaggaga gaacacccgc ttgttacacc
     5401 ctgtgagcct gcatgggatg gatgacccgg agagagaagt attagagtgg aggtttgaca
     5461 gccgcctagc atttcatcac atggcccgag agctgcatcc ggactgtact gggtctctct
     5521 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     5581 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     5641 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agggcccgtt
     5701 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     5761 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     5821 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     5881 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     5941 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
     6001 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     6061 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     6121 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     6181 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     6241 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     6301 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     6361 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     6421 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     6481 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     6541 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     6601 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     6661 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
     6721 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
     6781 gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat
     6841 cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt
     6901 tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg
     6961 gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct
     7021 gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt
     7081 gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga
     7141 cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct
     7201 gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact gacacgtgct
     7261 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
     7321 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
     7381 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     7441 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     7501 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct
     7561 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     7621 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     7681 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     7741 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     7801 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     7861 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     7921 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     7981 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     8041 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     8101 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     8161 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     8221 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     8281 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     8341 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     8401 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     8461 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     8521 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     8581 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     8641 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     8701 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     8761 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     8821 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     8881 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     8941 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     9001 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     9061 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     9121 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     9181 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     9241 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     9301 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     9361 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     9421 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     9481 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     9541 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     9601 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     9661 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg
     9721 agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     9781 agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt
     9841 taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg
     9901 cgttttgcgc tgcttcg
//
