LOCUS       sequence-414895-       10955 bp    DNA     circular SYN 15-APR-2025
DEFINITION  doxycycline-inducible expression of Human TRPS1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10955)
  AUTHORS   Altman A, Henriques-Oliveira L, Kurochkin I, Ascic E, Halitzki E, 
            Rosa F, Pires C, Pereira C-F
  TITLE     Transcription Factor Blueprints Underlying Dendritic Cell Diversity
  JOURNAL   Exp Hematol. 2024 Aug. 137:104298
REFERENCE   2  (bases 1 to 10955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Exp 
            Hematol. 2024 Aug. 137:104298"
COMMENT     Sequence Label: pFUW-TetO-TRPS1
FEATURES             Location/Qualifiers
     source          1..10955
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        27..406
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        408..606
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             624..804
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    851..976
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1469..1702
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1887..1931
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2229..2346
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2403..2421,2422..2444,2445..2463,2464..2486,2487..2505,
                     2506..2528,2529..2547,2548..2570,2571..2589,2590..2612,
                     2613..2631,2632..2654,2655..2673)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2706..2744
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2741..2765
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    5436..6024
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5489..5509)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5907..5918)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6027..6043)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6028..6044)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6549..6729
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6755..6772)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6761..6985
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7031..7459
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7118..7137)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7328..7349
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7468..7488)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7473..7802
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7653..7788
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7715..7734
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7850..7897
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7916..8290
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8420..8541
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8457..8476)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8511..8530
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8590..8606)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8590..8606)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8603..8625)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8614..8630
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8638..8644,8645..8662,8663..8668))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8683..8704
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8821..8838)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8992..9580)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9072..9091)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9751..10542,10543..10611))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10374..10393
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10612..10716)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10791..10810)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
       61 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      121 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      181 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      241 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      301 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      361 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
      421 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
      481 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
      541 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      601 aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
      661 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
      721 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
      781 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
      841 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
      901 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
      961 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     1021 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     1081 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     1141 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     1201 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     1261 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     1321 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     1381 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     1441 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     1501 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     1561 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     1621 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     1681 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     1741 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     1801 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     1861 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     1921 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     1981 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     2041 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     2101 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
     2161 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
     2221 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     2281 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     2341 aattttcggg tttattacag ggacagcaga gatccagttt ggttagatct cgagtttacc
     2401 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     2461 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     2521 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
     2581 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
     2641 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagct cggtacccgg
     2701 gtcgaggtag gcgtgtacgg tgggaggcct atataagcag agctcgttta gtgaaccgtc
     2761 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
     2821 ccagcctccg cggccccgaa ttcaccggta tggtccggaa aaagaacccc cctctgagaa
     2881 acgttgcaag tgaaggcgag ggccagatcc tggagcctat aggtacagaa agcaaggtat
     2941 ctggaaagaa caaagaattt tctgcagatc agatgtcaga aaatacggat cagagtgatg
     3001 ctgcagaact aaatcataag gaggaacata gcttgcatgt tcaagatcca tcttctagca
     3061 gtaagaagga cttgaaaagc gcagttctga gtgagaaggc tggcttcaat tatgaaagcc
     3121 ccagtaaggg aggaaacttt ccctcctttc cgcatgatga ggtgacagac agaaatatgt
     3181 tggctttctc atctccagct gctgggggag tctgtgagcc cttgaagtct ccgcaaagag
     3241 cagaggcaga tgaccctcaa gatatggcct gcaccccctc aggggactca ctggagacaa
     3301 aggaagatca gaagatgtca ccaaaggcta cagaggaaac agggcaagca cagagtggtc
     3361 aagccaattg tcaaggtttg agcccagttt cagtggcctc aaaaaaccca caagtgcctt
     3421 cagatggggg tgtaagactg aataaatcca aaactgactt actggtgaat gacaacccag
     3481 acccggcacc tctgtctcca gagcttcagg actttaaatg caatatctgt ggatatggtt
     3541 actacggcaa cgaccccaca gatctgatta agcacttccg aaagtatcac ttaggactgc
     3601 ataaccgcac caggcaagat gctgagctgg acagcaaaat cttggccctt cataacatgg
     3661 tgcagttcag ccattccaaa gacttccaga aggtcaaccg ttctgtgttt tctggtgtgc
     3721 tgcaggacat caattcttca aggcctgttt tactaaatgg gacctacgat gtgcaggtga
     3781 cttcaggtgg aacattcatt ggcattggac ggaaaacacc agattgccaa gggaacacca
     3841 agtatttccg ctgtaaattc tgcaatttca cttatatggg caattcatcc accgaattag
     3901 aacaacattt tcttcagact cacccaaaca aaataaaagc ttctctcccc tcctctgagg
     3961 ttgcaaaacc ttcagagaaa aactctaaca agtccatccc tgcacttcaa tccagtgatt
     4021 ctggagactt gggaaaatgg caggacaaga taacagtcaa agcaggagat gacactcctg
     4081 ttgggtactc agtgcccata aagcccctcg attcctctag acaaaatggt acagaggcca
     4141 ccagttacta ctggtgtaaa ttttgtagtt tcagctgtga gtcatctagc tcacttaaac
     4201 tgctagaaca ttatggcaag cagcacggag cagtgcagtc aggcggcctt aatccagagt
     4261 taaatgataa gctttccagg ggctctgtca ttaatcagaa tgatctagcc aaaagttcag
     4321 aaggagagac aatgaccaag acagacaaga gctcgagtgg ggctaaaaag aaggacttct
     4381 ccagcaaggg agccgaggat aatatggtaa cgagctataa ttgtcagttc tgtgacttcc
     4441 gatattccaa aagccatggc cctgatgtaa ttgtagtggg gccacttctc cgtcattatc
     4501 aacagctcca taacattcac aagtgtacca ttaaacactg tccattctgt cccagaggac
     4561 tttgcagccc agaaaagcac cttggagaaa ttacttatcc gtttgcttgt agaaaaagta
     4621 attgttccca ctgtgcactc ttgcttctgc acttgtctcc tggggcggct ggaagctcgc
     4681 gagtcaaaca tcagtgccat cagtgttcat tcaccacccc tgacgtagat gtactcctct
     4741 ttcactatga aagtgtgcat gagtcccaag catcggatgt caaacaagaa gcaaatcacc
     4801 tgcaaggatc ggatgggcag cagtctgtca aggaaagcaa agaacactca tgtaccaaat
     4861 gtgattttat tacccaagtg gaagaagaga tttcccgaca ctacaggaga gcacacagct
     4921 gctacaaatg ccgtcagtgc agttttacag ctgccgatac tcagtcacta ctggagcact
     4981 tcaacactgt tcactgccag gaacaggaca tcactacagc caacggcgaa gaggacggtc
     5041 atgccatatc caccatcaaa gaggagccca aaattgactt cagggtctac aatctgctaa
     5101 ctccagactc taaaatggga gagccagttt ctgagagtgt ggtgaagaga gagaagctgg
     5161 aagagaagga cgggctcaaa gagaaagttt ggaccgagag ttccagtgat gaccttcgca
     5221 atgtgacttg gagaggggca gacatcctgc gggggagtcc gtcatacacc caagcaagcc
     5281 tggggctgct gacgcctgtg tctggcaccc aagagcagac aaagactcta agggatagtc
     5341 ccaatgtgga ggccgcccat ctggcgcgac ctatttatgg cttggctgtg gaaaccaagg
     5401 gattcctgta gttcgaagat atcaagctta tcgataatca acctctggat tacaaaattt
     5461 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     5521 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     5581 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     5641 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     5701 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     5761 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     5821 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
     5881 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     5941 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     6001 tctccctttg ggccgcctcc ccgcatcgat accgtcgacc tcgagaccta gaaaaacatg
     6061 gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac
     6121 aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt
     6181 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     6241 ttcactccca acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact
     6301 tccctgattg gcagaactac acaccagggc cagggatcag atatccactg acctttggat
     6361 ggtgctacaa gctagtacca gttgagcaag agaaggtaga agaagccaat gaaggagaga
     6421 acacccgctt gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtat
     6481 tagagtggag gtttgacagc cgcctagcat ttcatcacat ggcccgagag ctgcatccgg
     6541 actgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     6601 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     6661 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     6721 tctctagcag ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
     6781 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     6841 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     6901 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     6961 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg
     7021 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     7081 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     7141 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     7201 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     7261 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     7321 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     7381 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     7441 caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     7501 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     7561 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     7621 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     7681 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     7741 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     7801 aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta
     7861 atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc
     7921 caagttgacc agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt
     7981 ctggaccgac cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt
     8041 ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac
     8101 cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt
     8161 gtccacgaac ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg
     8221 ggggcgggag ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga
     8281 gcaggactga cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     8341 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     8401 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
     8461 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     8521 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
     8581 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     8641 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     8701 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     8761 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     8821 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     8881 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     8941 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     9001 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     9061 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     9121 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     9181 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     9241 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     9301 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     9361 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     9421 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     9481 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     9541 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     9601 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     9661 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     9721 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     9781 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     9841 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     9901 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     9961 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
    10021 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
    10081 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
    10141 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
    10201 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
    10261 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
    10321 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
    10381 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
    10441 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
    10501 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
    10561 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
    10621 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
    10681 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
    10741 tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc
    10801 tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag
    10861 tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag
    10921 aatctgctta gggttaggcg ttttgcgctg cttcg
//
