LOCUS       sequence-414898-       10163 bp    DNA     circular SYN 15-APR-2025
DEFINITION  doxycycline-inducible expression of Human ASB2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10163)
  AUTHORS   Altman A, Henriques-Oliveira L, Kurochkin I, Ascic E, Halitzki E, 
            Rosa F, Pires C, Pereira C-F
  TITLE     Transcription Factor Blueprints Underlying Dendritic Cell Diversity
  JOURNAL   Exp Hematol. 2024 Aug. 137:104298
REFERENCE   2  (bases 1 to 10163)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Exp 
            Hematol. 2024 Aug. 137:104298"
COMMENT     Sequence Label: pFUW-TetO-ASB2
FEATURES             Location/Qualifiers
     source          1..10163
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        27..406
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        408..606
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             624..804
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    851..976
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1469..1702
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1887..1931
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2229..2346
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2403..2421,2422..2444,2445..2463,2464..2486,2487..2505,
                     2506..2528,2529..2547,2548..2570,2571..2589,2590..2612,
                     2613..2631,2632..2654,2655..2673)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2706..2744
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2741..2765
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    4644..5232
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4697..4717)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5115..5126)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5235..5251)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5236..5252)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5757..5937
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5963..5980)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5969..6193
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6239..6667
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6326..6345)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6536..6557
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6676..6696)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6681..7010
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6861..6996
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6923..6942
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7058..7105
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7124..7498
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7628..7749
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7665..7684)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7719..7738
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7798..7814)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7798..7814)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7811..7833)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7822..7838
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7846..7852,7853..7870,7871..7876))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7891..7912
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8029..8046)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8200..8788)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8280..8299)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8959..9750,9751..9819))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9582..9601
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9820..9924)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9999..10018)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
       61 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      121 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      181 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      241 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      301 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      361 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
      421 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
      481 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
      541 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      601 aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
      661 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
      721 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
      781 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
      841 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
      901 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
      961 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     1021 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     1081 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     1141 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     1201 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     1261 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     1321 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     1381 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     1441 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     1501 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     1561 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     1621 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     1681 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     1741 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     1801 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     1861 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     1921 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     1981 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     2041 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     2101 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
     2161 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
     2221 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     2281 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     2341 aattttcggg tttattacag ggacagcaga gatccagttt ggttagatct cgagtttacc
     2401 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     2461 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     2521 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
     2581 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
     2641 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagct cggtacccgg
     2701 gtcgaggtag gcgtgtacgg tgggaggcct atataagcag agctcgttta gtgaaccgtc
     2761 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
     2821 ccagcctccg cggccccgaa ttcaccggta tgacccgctt ctcctatgca gagtactttt
     2881 ccctctttca ctcctgctct gcaccctcca ggtccactgc acctcctgag agttcgccgg
     2941 cccgggcccc aatgggcttg ttccaagggg tcatgcagaa atacagcagc agcttgttca
     3001 agacctccca gctggcgcct gcggacccct tgataaaggc catcaaggat ggcgatgaag
     3061 aggccttgaa gaccatgatc aaggaaggga agaatctcgc agagcccaac aaggagggct
     3121 ggctgccgct gcacgaggcc gcatactatg gccaggtggg ctgcctgaaa gtcctgcagc
     3181 gagcgtaccc agggaccatc gaccagcgca ccctgcagga ggaaacagcc gtttacttgg
     3241 caacgtgcag gggccacctg gactgtctcc tgtcactgct ccaagcaggg gcagagccgg
     3301 acatctccaa caaatcccga gagacaccgc tctacaaagc ctgcgagcgc aagaacgcgg
     3361 aggccgtgaa gattctggtg cagcacaacg cagacaccaa ccaccgctgc aaccgcggct
     3421 ggaccgctct gcacgagtct gtgtctcgca atgacctgga ggtcatgcag atcctggtga
     3481 gcggaggagc caaggtggaa tccaagaacg cctacggcat cacccccttg ttcgtggccg
     3541 cccagagtgg acagttggag gccttgaggt tcttagccaa gtacggtgct gacatcaaca
     3601 cgcaggccag cgacaacgcg tctgccctct acgaggcctg caagaatgag catgaggagg
     3661 tggtggagtt tctgctgtca cagggtgccg acgccaacaa gaccaacaag gacggcttgc
     3721 tcccgctgca catcgcctcc aagaagggca actacaggat cgtgcagatg ctgctgccgg
     3781 tgaccagccg cacgcgcata cgccgtagcg gcgtcagtcc gctgcacctg gcggccgagc
     3841 gcaaccacga cgaggtgctg gaggcgctgc tgagcgcgcg cttcgacgtg aacacgccgc
     3901 tggcgcccga gcgcgcgcgc ctctacgaag accggcgcag ctccgcgctg tacttcgcgg
     3961 tggtcaacaa caacgtgtac gccaccgagc tgctgctgca acacggcgcc gaccccaacc
     4021 gcgacgtcat cagccccttg ctcgtggcca tccgccacgg ctgcctgcgc acaatgcagc
     4081 tgctgctgga ccacggcgcg aacatcgacg cctatatcgc cacgcacccc accgccttcc
     4141 ccgccaccat catgttcgcc atgaagtgcc tgtcgctgct caagttcctc atggacctgg
     4201 gctgcgacgg cgagccctgc ttctcatgcc tctacggcaa cggcccgcac ccgccggccc
     4261 cgcagccctc cagcaggttc aacgacgcgc ccgcggccga caaggagccc agcgtggtgc
     4321 agttctgtga gttcgtatct gccccagagg tgagccgctg ggcggggccc atcatcgatg
     4381 tcctcctgga ctacgtgggc aacgtgcagc tctgctcgcg gctgaaggaa cacatcgaca
     4441 gctttgagga ctgggccgtc atcaaggaga aggcagaacc tccaagacct ctggctcacc
     4501 tttgccgact gcgggttcga aaggccattg ggaaataccg tataaaactc ctagacacct
     4561 tgccgctccc aggcaggctg attagatacc tgaaatacga gaacacccag taagctagct
     4621 tcgaagatat caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga
     4681 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     4741 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     4801 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     4861 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     4921 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     4981 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     5041 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     5101 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     5161 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     5221 ccgcctcccc gcatcgatac cgtcgacctc gagacctaga aaaacatgga gcaatcacaa
     5281 gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg
     5341 aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg
     5401 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     5461 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattggc
     5521 agaactacac accagggcca gggatcagat atccactgac ctttggatgg tgctacaagc
     5581 tagtaccagt tgagcaagag aaggtagaag aagccaatga aggagagaac acccgcttgt
     5641 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtatta gagtggaggt
     5701 ttgacagccg cctagcattt catcacatgg cccgagagct gcatccggac tgtactgggt
     5761 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     5821 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     5881 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcaggg
     5941 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     6001 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     6061 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     6121 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     6181 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
     6241 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     6301 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     6361 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     6421 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     6481 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     6541 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     6601 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     6661 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     6721 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     6781 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     6841 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     6901 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
     6961 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
     7021 gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata
     7081 gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca agttgaccag
     7141 tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg
     7201 gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt
     7261 gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt
     7321 gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt
     7381 ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt
     7441 cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca
     7501 cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     7561 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     7621 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     7681 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     7741 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
     7801 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     7861 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     7921 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     7981 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     8041 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     8101 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     8161 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     8221 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     8281 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     8341 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     8401 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     8461 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     8521 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     8581 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     8641 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     8701 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     8761 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     8821 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     8881 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     8941 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     9001 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     9061 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     9121 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     9181 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     9241 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     9301 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     9361 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     9421 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     9481 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     9541 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     9601 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     9661 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     9721 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     9781 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     9841 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     9901 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg
     9961 atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca
    10021 tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc
    10081 aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg
    10141 gttaggcgtt ttgcgctgct tcg
//
