LOCUS       sequence-414903-       12050 bp    DNA     circular SYN 15-APR-2025
DEFINITION  doxycycline-inducible expression of Human ZEB in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12050)
  AUTHORS   Altman A, Henriques-Oliveira L, Kurochkin I, Ascic E, Halitzki E, 
            Rosa F, Pires C, Pereira C-F
  TITLE     Transcription Factor Blueprints Underlying Dendritic Cell Diversity
  JOURNAL   Exp Hematol. 2024 Aug. 137:104298
REFERENCE   2  (bases 1 to 12050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Exp 
            Hematol. 2024 Aug. 137:104298"
COMMENT     Sequence Label: pFUW-TetO-ZEB2
FEATURES             Location/Qualifiers
     source          1..12050
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        27..406
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        408..606
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             624..804
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    851..976
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1469..1702
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1887..1931
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2229..2346
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2403..2421,2422..2444,2445..2463,2464..2486,2487..2505,
                     2506..2528,2529..2547,2548..2570,2571..2589,2590..2612,
                     2613..2631,2632..2654,2655..2673)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2706..2744
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2741..2765
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    6531..7119
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6584..6604)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7002..7013)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7122..7138)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7123..7139)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7644..7824
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7850..7867)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7856..8080
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8126..8554
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8213..8232)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8423..8444
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8563..8583)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8568..8897
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8748..8883
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8810..8829
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8945..8992
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9011..9385
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9515..9636
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9552..9571)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9606..9625
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9685..9701)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9685..9701)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9698..9720)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9709..9725
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9733..9739,9740..9757,9758..9763))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9778..9799
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9916..9933)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10087..10675)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10167..10186)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10846..11637,11638..11706))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11469..11488
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11707..11811)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11886..11905)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
       61 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      121 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      181 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      241 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      301 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      361 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
      421 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
      481 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
      541 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      601 aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
      661 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
      721 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
      781 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
      841 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
      901 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
      961 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     1021 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     1081 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     1141 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     1201 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     1261 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     1321 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     1381 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     1441 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     1501 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     1561 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     1621 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     1681 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     1741 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     1801 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     1861 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     1921 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     1981 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     2041 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     2101 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
     2161 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
     2221 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     2281 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     2341 aattttcggg tttattacag ggacagcaga gatccagttt ggttagatct cgagtttacc
     2401 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     2461 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     2521 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
     2581 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
     2641 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagct cggtacccgg
     2701 gtcgaggtag gcgtgtacgg tgggaggcct atataagcag agctcgttta gtgaaccgtc
     2761 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
     2821 ccagcctccg cggccccgaa ttcaccggtt ctagaatgaa gcagccgatc atggcggatg
     2881 gcccccggtg caagaggcgc aaacaagcca atcccaggag gaaaaacgtg gtgaactatg
     2941 acaatgtagt ggacacaggt tctgaaacag atgaggaaga caagcttcat attgctgagg
     3001 atgacggtat tgccaaccct ctggaccagg agacgagtcc agctagtgtg cccaaccatg
     3061 agtcctcccc acacgtgagc caagctctgt tgccaagaga ggaagaggaa gatgaaataa
     3121 gggagggtgg agtggaacac ccctggcaca acaacgagat tctacaagcc tctgtagatg
     3181 gtccagaaga aatgaaggaa gactatgaca ctatggggcc agaagccacg atccagaccg
     3241 caattaacaa tggtacagtg aagaatgcaa attgcacatc agattttgag gaatactttg
     3301 ccaaaagaaa actggaggaa cgcgatggtc atgcagtcag catcgaggag taccttcagc
     3361 gcagtgacac agccattatt tacccagaag cccctgagga gctgtctcgc cttggcacgc
     3421 cagaggccaa tgggcaagaa gaaaatgacc tgccacctgg aactccagat gcttttgccc
     3481 aactgctgac ctgcccctac tgcgaccggg gctacaagcg cttgacatca ctgaaggagc
     3541 acatcaagta ccgccacgag aagaatgaag agaacttttc ctgccctctc tgtagctaca
     3601 cgtttgccta ccgcacccag ctcgagcggc atatggtgac acacaagcca gggacagatc
     3661 agcaccaaat gctaacccaa ggagcaggta atcgcaagtt caaatgcaca gagtgtggca
     3721 aggccttcaa atataaacac catctgaaag aacacctgcg aattcacagt ggtgaaaaac
     3781 cttacgagtg cccaaactgc aagaaacgtt tctcccattc tggttcctac agttcgcaca
     3841 tcagcagcaa gaaatgtatt ggtttaatct ctgtaaatgg ccgaatgaga aacaatatca
     3901 agacgggttc ttcccctaat tctgtttctt cttctcctac taattcagcc attacccagt
     3961 taagaaacaa gttggagaat ggaaaaccac ttagtatgtc tgaacagaca ggcttactta
     4021 aaattaaaac agaaccacta gacttcaatg actataaagt tcttatggct acacacgggt
     4081 ttagtggcac tagtcccttt atgaatggtg ggcttggagc caccagccct ttaggagttc
     4141 atccatctgc tcagagtcca atgcagcact taggtgtagg gatggaagcc cctttacttg
     4201 ggtttcccac catgaatagt aatttaagtg aggtacaaaa ggttctacag attgtggaca
     4261 atactgtttc caggcaaaaa atggactgca aggctgaaga aatttcaaag ttgaaaggtt
     4321 atcacatgaa ggatccatgc tctcaacctg aggaacaagg agttacttct cctaatattc
     4381 cgcctgtcgg tcttccggta gtgagtcata atggtgccac taaaagtatt attgactata
     4441 cgttggaaaa agtcaatgaa gccaaagctt gcctccagag cttgactact gactcaagga
     4501 gacagatcag taatataaag aaagagaagc tacgtacttt aatagatttg gtcactgatg
     4561 acaaaatgat tgagaaccac aacatatcca ctccattttc atgccagttc tgtaaagaaa
     4621 gttttcctgg ccccatccct ttgcatcagc atgaacgtta cctttgtaag atgaatgaag
     4681 agatcaaggc ggtcctgcag cctcatgaaa acatagtccc caacaaagcc ggagtttttg
     4741 ttgataataa agccctcctc ttgtcatctg tactttctga gaaaggaatg acaagcccca
     4801 tcaacccata caaggaccac atgtctgtac tcaaagcata ctatgctatg aacatggagc
     4861 ccaactccga tgaactgctg aaaatttcca ttgctgtggg ccttcctcag gaatttgtga
     4921 aggaatggtt tgaacaacga aaagtctacc agtactcaaa ttccaggtcc ccatccctgg
     4981 aaagaagctc caagccgtta gctcccaaca gtaaccctcc cacaaaagac tctttattac
     5041 ccaggtctcc tgtaaaacct atggactcca taacatcacc atctatagca gaactccaca
     5101 acagtgttac gaattgtgat cctcctctca ggctaacaaa accttcccat tttaccaata
     5161 ttaaaccagt tgaaaaattg gaccactcca ggagtaatac tccttctccc ttaaatcttt
     5221 cctccacatc ttctaaaaac tcccacagta gttcatacac tccaaacagc ttctcttctg
     5281 aggagctcca ggctgagcct ttagacttgt cattaccaaa acaaatgaaa gaacccaaaa
     5341 gtattatagc cacaaagaac aaaacaaaag ctagtagcat cagtttagat cataacagtg
     5401 tttcttcctc atctgaaaac tcagatgagc ctctgaactt gacttttatc aagaaggaat
     5461 tttcaaattc aaataatctg gacaacaaaa gcactaaccc agtgttcagc atgaacccat
     5521 ttagtgccaa acctttatac acagctcttc cacctcaaag cgcatttccc cctgctactt
     5581 tcatgccacc agtccagacc agtattcctg ggctacgacc atacccagga ctggatcaga
     5641 tgagcttcct accacatatg gcctacacct acccaactgg agcagctact tttgctgata
     5701 tgcagcaaag gagaaagtac cagcggaaac aaggatttca gggagaattg cttgatggag
     5761 cacaagacta catgtcaggc ctagatgata tgacagactc cgactcctgt ctgtctcgca
     5821 aaaagatcaa gaagacagag agtggcatgt atgcatgtga cttatgtgac aagacattcc
     5881 agaaaagcag ttcccttctg cgacataaat acgaacacac aggaaaaaga ccacatcagt
     5941 gtcagatttg taagaaagcg tttaaacaca agcaccacct tatcgagcac tcaaggcttc
     6001 actcgggcga gaagccctat cagtgtgata aatgtggcaa gcgcttctca cactcgggct
     6061 cgtactcgca gcacatgaat cacaggtatt cctactgcaa gcgggaggcg gaggagcggg
     6121 aagcggcgga gcgcgaggcg cgcgagaaag ggcacttgga acccaccgag ctgctgatga
     6181 accgggctta cttgcagagc attacccctc aggggtactc tgactcggag gagagggaga
     6241 gtatgccgag ggatggcgag agcgagaagg agcacgagaa agaaggcgag gatggctacg
     6301 ggaagctggg cagacaggat ggcgacgagg agttcgagga ggaagaggaa gaaagtgaaa
     6361 ataaaagtat ggatacggat cccgaaacga tacgagatga agaagagact ggagatcact
     6421 ccatggacga tagttcggag gatgggaaaa tggaaaccaa atcagaccac gaggaagaca
     6481 atatggaaga tggcatgtaa gctagcttcg aagatatcaa gcttatcgat aatcaacctc
     6541 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     6601 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     6661 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     6721 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     6781 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     6841 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     6901 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     6961 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     7021 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     7081 ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgag
     7141 acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg
     7201 cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt
     7261 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     7321 gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc
     7381 acacacaagg ctacttccct gattggcaga actacacacc agggccaggg atcagatatc
     7441 cactgacctt tggatggtgc tacaagctag taccagttga gcaagagaag gtagaagaag
     7501 ccaatgaagg agagaacacc cgcttgttac accctgtgag cctgcatggg atggatgacc
     7561 cggagagaga agtattagag tggaggtttg acagccgcct agcatttcat cacatggccc
     7621 gagagctgca tccggactgt actgggtctc tctggttaga ccagatctga gcctgggagc
     7681 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     7741 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     7801 agtcagtgtg gaaaatctct agcagggccc gtttaaaccc gctgatcagc ctcgactgtg
     7861 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     7921 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     7981 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     8041 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     8101 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
     8161 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     8221 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     8281 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     8341 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     8401 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     8461 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     8521 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag
     8581 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     8641 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     8701 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     8761 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     8821 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     8881 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc
     8941 acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag
     9001 gaactaaacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc
     9061 cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga
     9121 cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt
     9181 ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga
     9241 gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat
     9301 cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca
     9361 cttcgtggcc gaggagcagg actgacacgt gctacgagat ttcgattcca ccgccgcctt
     9421 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     9481 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
     9541 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     9601 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
     9661 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     9721 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     9781 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     9841 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     9901 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     9961 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
    10021 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
    10081 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
    10141 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
    10201 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
    10261 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
    10321 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
    10381 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
    10441 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
    10501 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
    10561 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
    10621 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
    10681 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
    10741 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
    10801 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
    10861 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
    10921 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
    10981 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
    11041 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
    11101 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
    11161 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
    11221 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
    11281 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
    11341 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
    11401 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
    11461 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
    11521 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
    11581 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    11641 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    11701 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    11761 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    11821 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact
    11881 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg
    11941 ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc
    12001 gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg
//
