LOCUS       sequence-414906-       11966 bp    DNA     circular SYN 15-APR-2025
DEFINITION  doxycycline-inducible expression of Human ARID5B in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11966)
  AUTHORS   Altman A, Henriques-Oliveira L, Kurochkin I, Ascic E, Halitzki E, 
            Rosa F, Pires C, Pereira C-F
  TITLE     Transcription Factor Blueprints Underlying Dendritic Cell Diversity
  JOURNAL   Exp Hematol. 2024 Aug. 137:104298
REFERENCE   2  (bases 1 to 11966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Exp 
            Hematol. 2024 Aug. 137:104298"
COMMENT     Sequence Label: pFUW-TetO-ARID5B
FEATURES             Location/Qualifiers
     source          1..11966
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        27..406
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        408..606
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             624..804
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    851..976
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1469..1702
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1887..1931
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2229..2346
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2403..2421,2422..2444,2445..2463,2464..2486,2487..2505,
                     2506..2528,2529..2547,2548..2570,2571..2589,2590..2612,
                     2613..2631,2632..2654,2655..2673)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2706..2744
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2741..2765
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    6447..7035
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6500..6520)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6918..6929)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7038..7054)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7039..7055)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7560..7740
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7766..7783)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7772..7996
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8042..8470
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8129..8148)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8339..8360
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8479..8499)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8484..8813
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8664..8799
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8726..8745
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8861..8908
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8927..9301
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9431..9552
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9468..9487)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9522..9541
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9601..9617)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9614..9636)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9625..9641
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9649..9655,9656..9673,9674..9679))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9694..9715
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9832..9849)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10003..10591)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10083..10102)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10762..11553,11554..11622))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11385..11404
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11623..11727)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11802..11821)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
       61 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      121 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      181 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      241 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      301 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      361 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
      421 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
      481 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
      541 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      601 aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
      661 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
      721 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
      781 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
      841 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
      901 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
      961 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     1021 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     1081 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     1141 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     1201 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     1261 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     1321 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     1381 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     1441 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     1501 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     1561 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     1621 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     1681 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     1741 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     1801 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     1861 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     1921 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     1981 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     2041 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     2101 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
     2161 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
     2221 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     2281 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     2341 aattttcggg tttattacag ggacagcaga gatccagttt ggttagatct cgagtttacc
     2401 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     2461 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     2521 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
     2581 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
     2641 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagct cggtacccgg
     2701 gtcgaggtag gcgtgtacgg tgggaggcct atataagcag agctcgttta gtgaaccgtc
     2761 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
     2821 ccagcctccg cggccccgaa ttcaccggtt ctagaatgga gcccaactca ctccagtggg
     2881 tcggctcacc gtgtggcttg cacggacctt acattttcta caaggctttt caattccacc
     2941 ttgaaggcaa accaagaatt ttgtcccttg gcgacttttt ctttgtaaga tgtacgccaa
     3001 aggatccgat ttgcatagcg gagctccagc tgttgtggga agagaggacc agccggcaac
     3061 ttttatccag ctctaaactt tatttcctcc cagaagacac tccccagggc agaaatagcg
     3121 accatggcga ggatgaagtc attgctgttt ccgaaaaggt gattgtgaag cttgaagacc
     3181 tggtcaagtg ggtacattct gatttctcca agtggagatg tggcttccac gctggaccag
     3241 tgaaaactga ggccttggga aggaatggac agaaggaagc tctgctgaag tacaggcagt
     3301 caaccctaaa cagtggactc aacttcaaag acgttctcaa ggagaaggca gacctggggg
     3361 aggacgagga agaaacgaac gtgatagttc tcagctaccc ccagtactgc cggtaccgct
     3421 cgatgctgaa acgcatccag gataagccat cttccattct aacggaccag tttgcattgg
     3481 ccctgggggg cattgcagtg gtcagcagga accctcagat cctgtactgt cgggacacct
     3541 ttgaccaccc gactctcata gaaaacgaga gtatatgcga tgagtttgcg ccaaatctta
     3601 aaggcagacc acgcaaaaag aaaccatgcc cacaaagaag agattcattc agtggtgtta
     3661 aggattccaa caacaattcc gatggcaaag ccgttgccaa ggtgaaatgt gaggccaggt
     3721 cagccttgac caagccgaag aataaccata actgtaaaaa agtctcaaat gaagaaaaac
     3781 caaaggttgc cattggtgaa gagtgcaggg cagatgaaca agccttcttg gtggcacttt
     3841 ataaatacat gaaagaaagg aaaacgccga tagaacgaat accctattta ggttttaaac
     3901 agattaacct ttggactatg tttcaagctg ctcaaaaact gggaggatat gaaacaataa
     3961 cagcccgccg tcagtggaaa catatttatg atgaattagg cggtaatcct gggagcacca
     4021 gcgctgccac ttgtacccgc agacattatg aaagattaat cctaccatat gaaagattta
     4081 ttaaaggaga agaagataag cccctgcctc caatcaaacc tcggaaacag gagaacagtt
     4141 cacaggaaaa tgagaacaaa acaaaagtat ctggaaccaa acgcatcaaa catgaaatac
     4201 ctaaaagcaa gaaagaaaaa gaaaatgccc caaagcccca ggatgcagca gaggtttcat
     4261 cagagcaaga aaaagaacaa gagactttaa taagccagaa aagcatccct gagcctctcc
     4321 cagcagcaga catgaagaaa aaaatagaag ggtatcagga attttcagcg aagcccctgg
     4381 catccagagt agacccagag aaggacaacg aaacagacca aggttccaac agtgagaagg
     4441 tggcagagga ggcgggagag aaggggccca cacctccact cccaagtgct cctctggccc
     4501 cagaaaaaga ttcagccttg gtccctgggg ccagcaaaca gccactcacc tctcctagtg
     4561 ccctggtgga ctcaaaacaa gaatccaaac tgtgctgttt tacagagagc cctgaaagtg
     4621 aaccccaaga agcatccttc cccagcttcc ccaccacaca gccaccgctg gcaaaccaga
     4681 atgagacgga ggatgacaaa ctgcccgcca tggcagatta cattgccaac tgcaccgtga
     4741 aggtggacca gctgggcagt gacgacatcc acaatgcgct caagcagacc ccaaaggtcc
     4801 ttgtggtcca gtcgtttgac atgttcaaag acaaagacct gactgggccc atgaacgaga
     4861 accatggact taattacacg cccctgctct actctagggg caacccaggc atcatgtccc
     4921 cactggccaa gaaaaagctt ttgtcccaag tgagtggggc cagcctctcc agcagctacc
     4981 cttatggctc cccaccccct ttgatcagca aaaagaaact gattgctagg gatgacttgt
     5041 gttccagttt gtcccagacc caccatggcc aaagcactga ccatatggcg gtcagccggc
     5101 catcagtgat tcagcacgtc cagagtttca gaagcaagcc ctcggaagag agaaagacca
     5161 tcaatgacat ctttaagcat gagaaactga gtcgatcaga tccccaccgc tgcagcttct
     5221 ccaagcatca ccttaacccc cttgctgact cctacgtcct gaagcaagaa attcaggagg
     5281 gcaaggataa actcttagag aaaagggccc tcccccattc ccacatgcct agcttcctgg
     5341 ctgacttcta ctcgtcccct catctccata gcctctacag acacaccgag caccatcttc
     5401 ataatgaaca gacatccaaa tacccttcca gggacatgta cagggaatcg gaaaacagtt
     5461 cttttccttc ccacagacac caagaaaagc tccatgtaaa ttatctcacg tccctgcacc
     5521 tgcaagacaa aaagtcggcg gcagcagaag cccctacgga tgatcagcct acagatctga
     5581 gccttcccaa gaacccgcac aaacctaccg gcaaggtcct gggcctggct cattccacca
     5641 cagggcccca ggagagcaaa ggcatctccc agttccaggt cttaggcagc cagagtcgag
     5701 actgtcaccc caaagcctgt cgggtatcac ccatgaccat gtcaggccct aaaaaatacc
     5761 ctgaatcgct ttcaagatca ggaaaacctc accatgtgag actggagaat ttcaggaaga
     5821 tggaaggcat ggtccaccca atcctgcacc ggaaaatgag cccgcagaac attggggcgg
     5881 cgcggccgat caagcgcagc ctggaggatt tggaccttgt gattgcaggg aaaaaggccc
     5941 gggcagtgtc tcccttagac ccatccaagg aggtctctgg gaaggagaag gcctctgagc
     6001 aggagagtga aggcagcaaa gcagcgcacg gtgggcattc cgggggcgga tcagaaggcc
     6061 acaagcttcc cctctcctcc cctatcttcc caggtctgta ttccgggagc ctgtgtaact
     6121 cgggcctcaa ctccaggctc ccggctgggt attctcattc tctgcagtac ttgaaaaacc
     6181 agactgtgct ttctccactc atgcagcccc tggctttcca ctcgcttgtg atgcaaagag
     6241 gaatttttac atcaccgaca aattctcagc agctgtacag acacttggct gcggctacac
     6301 ctgtaggaag ttcatatggg gaccttttgc ataacagcat ttacccttta gctgctataa
     6361 atcctcaagc tgcctttcca tcttcccagc tgtcatccgt gcaccccagt acaaaactgt
     6421 aattcgaaga tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat
     6481 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     6541 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     6601 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     6661 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     6721 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     6781 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     6841 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     6901 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     6961 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     7021 gggccgcctc cccgcatcga taccgtcgac ctcgagacct agaaaaacat ggagcaatca
     7081 caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg
     7141 aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag
     7201 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     7261 aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
     7321 ggcagaacta cacaccaggg ccagggatca gatatccact gacctttgga tggtgctaca
     7381 agctagtacc agttgagcaa gagaaggtag aagaagccaa tgaaggagag aacacccgct
     7441 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagta ttagagtgga
     7501 ggtttgacag ccgcctagca tttcatcaca tggcccgaga gctgcatccg gactgtactg
     7561 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     7621 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     7681 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     7741 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     7801 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     7861 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     7921 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     7981 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
     8041 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     8101 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     8161 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     8221 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     8281 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     8341 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     8401 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     8461 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     8521 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     8581 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     8641 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     8701 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg
     8761 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
     8821 cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc
     8881 atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagttgac
     8941 cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga
     9001 ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga
     9061 cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg
     9121 ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa
     9181 cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga
     9241 gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg
     9301 acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     9361 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     9421 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     9481 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     9541 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
     9601 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     9661 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     9721 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     9781 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     9841 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     9901 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     9961 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
    10021 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
    10081 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
    10141 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
    10201 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
    10261 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
    10321 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
    10381 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
    10441 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
    10501 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
    10561 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
    10621 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
    10681 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
    10741 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
    10801 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
    10861 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
    10921 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
    10981 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
    11041 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
    11101 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
    11161 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    11221 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    11281 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    11341 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    11401 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    11461 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    11521 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    11581 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    11641 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    11701 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga
    11761 cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc
    11821 gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg
    11881 agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt
    11941 agggttaggc gttttgcgct gcttcg
//
