LOCUS       sequence-415032-       10640 bp    DNA     circular SYN 15-APR-2025
DEFINITION  doxycycline-inducible expression of Human Stat4 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10640)
  AUTHORS   Altman A, Henriques-Oliveira L, Kurochkin I, Ascic E, Halitzki E, 
            Rosa F, Pires C, Pereira C-F
  TITLE     Transcription Factor Blueprints Underlying Dendritic Cell Diversity
  JOURNAL   Exp Hematol. 2024 Aug. 137:104298
REFERENCE   2  (bases 1 to 10640)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Exp 
            Hematol. 2024 Aug. 137:104298"
COMMENT     Sequence Label: pFUW-TetO-Stat4
FEATURES             Location/Qualifiers
     source          1..10640
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        27..406
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        408..606
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             624..804
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    851..976
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1469..1702
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1887..1931
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2229..2346
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2403..2421,2422..2444,2445..2463,2464..2486,2487..2505,
                     2506..2528,2529..2547,2548..2570,2571..2589,2590..2612,
                     2613..2631,2632..2654,2655..2673)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2706..2744
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2741..2765
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    5121..5709
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5174..5194)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5592..5603)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5712..5728)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5713..5729)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6234..6414
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6440..6457)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6446..6670
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6716..7144
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6803..6822)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7013..7034
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7153..7173)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7158..7487
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7338..7473
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7400..7419
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7535..7582
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7601..7975
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8105..8226
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8142..8161)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8196..8215
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8275..8291)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8275..8291)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8288..8310)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8299..8315
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8323..8329,8330..8347,8348..8353))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8368..8389
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8506..8523)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8677..9265)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8757..8776)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9436..10227,10228..10296))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10059..10078
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10297..10401)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10476..10495)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
       61 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      121 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      181 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      241 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      301 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      361 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
      421 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
      481 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
      541 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      601 aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
      661 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
      721 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
      781 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
      841 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
      901 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
      961 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     1021 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     1081 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     1141 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     1201 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     1261 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     1321 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     1381 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     1441 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     1501 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     1561 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     1621 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     1681 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     1741 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     1801 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     1861 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     1921 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     1981 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     2041 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     2101 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
     2161 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
     2221 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     2281 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     2341 aattttcggg tttattacag ggacagcaga gatccagttt ggttagatct cgagtttacc
     2401 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     2461 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     2521 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
     2581 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
     2641 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagct cggtacccgg
     2701 gtcgaggtag gcgtgtacgg tgggaggcct atataagcag agctcgttta gtgaaccgtc
     2761 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
     2821 ccagcctccg cggccccgaa ttcaccggta tgtctcagtg gaatcaagtc caacagttag
     2881 aaatcaagtt tttggagcag gtggatcaat tctatgatga caactttccc atggaaattc
     2941 ggcatctgtt ggcccaatgg attgaaaatc aagactggga ggcagcttct aacaatgaaa
     3001 ccatggcaac gattcttctt caaaacttgt taatacaact ggatgaacag ttaggtcgtg
     3061 tttccaaaga gaaaaaccta ctcttgatac acaatctaaa aagaattagg aaggtccttc
     3121 agggaaaatt tcatggaaat ccaatgcatg tagctgtggt tatttcaaac tgtttaaggg
     3181 aagagaggag aatattggct gcagccaaca tgcctgtcca ggggcctcta gagaaatcct
     3241 tacaaagttc ttcagtttca gaaagacaga ggaatgtgga gcacaaagtg gctgccatta
     3301 aaaacagtgt gcagatgaca gaacaagata ccaaatactt agaagatctg caagacgaat
     3361 ttgactacag gtataaaaca attcagacaa tggatcagag tgacaagaat agtgccatgg
     3421 tgaatcagga agttttgaca ctgcaggaaa tgcttaacag cctcgatttc aagagaaagg
     3481 aggctctcag taaaatgacc caaatcatcc atgagacaga cctgttaatg aacaccatgc
     3541 tcatagaaga gctgcaagac tggaagcggc ggcagcaaat cgcctgcatc gggggtccac
     3601 tccacaatgg gctcgaccag cttcagaact gctttacact attggcagaa agtcttttcc
     3661 aactgagaag gcaattggag aaactagagg agcaatctac caaaatgaca tatgaaggtg
     3721 atcccattcc aatgcaaaga actcacatgc tagaaagagt caccttcttg atctacaacc
     3781 ttttcaagaa ctcatttgtg gttgagcgac agccatgtat gccaacccac cctcagaggc
     3841 cgttggtact taaaacccta attcagttca ctgtaaaact aaggctacta ataaaattgc
     3901 cagaactaaa ctatcaggta aaggttaagg catcaattga caagaatgtt tcaactctaa
     3961 gcaaccgaag atttgtactt tgtggaacta atgtcaaagc catgtctatt gaagaatctt
     4021 ccaatgggag tctctcagta gaatttcgac atttgcaacc aaaggaaatg aagtccagtg
     4081 ctggaggtaa aggaaatgag ggctgtcaca tggtgactga agaacttcat tccataacgt
     4141 ttgaaacaca gatctgcctc tatggcctga ccatagattt ggagaccagc tcattgcctg
     4201 tggtgatgat ttccaatgtc agtcagttac ctaatgcttg ggcatccatc atttggtaca
     4261 acgtgtcaac caacgattcc cagaacttgg ttttctttaa taatcctcca cctgccacat
     4321 tgagtcaact actggaggtg atgagctggc agttttcatc gtacgttggt cgtggtctta
     4381 actcagatca actccatatg ctggcagaga agcttacagt ccaatctagc tacagtgatg
     4441 gtcacctcac ctgggccaag ttctgcaagg aacatttacc tggtaaatca tttacctttt
     4501 ggacatggct tgaagcaata ttggatctaa ttaagaaaca cattcttccc ctttggattg
     4561 atgggtatgt catgggcttt gttagcaaag agaaggaacg gctgttgcta aaggataaaa
     4621 tgcctggcac ctttttatta agattcagtg aaagccatct cggaggaata actttcacct
     4681 gggtggacca ttctgaaagt ggggaagtga gattccactc tgtagaaccc tacaataaag
     4741 gccggttgtc tgctctgcca ttcgctgaca tcctgcgaga ctacaaagtt attatggctg
     4801 aaaacattcc tgaaaaccct ctgaagtacc tatatcctga cattcccaaa gacaaagcct
     4861 tcggtaaaca ctacagctct cagccttgcg aagtttcaag accaacagaa aggggtgaca
     4921 aaggttatgt tccttctgtt tttatcccca tctcaacaat ccgaagtgat tcaacagagc
     4981 cacattctcc atcagacctt cttcccatgt ctccaagtgt gtatgcggtg ttgagagaaa
     5041 acctgagtcc cacaacaatt gaaactgcaa tgaagtctcc ttattctgct gaatgattcg
     5101 aagatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg
     5161 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     5221 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     5281 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     5341 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     5401 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     5461 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     5521 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     5581 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     5641 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     5701 cctccccgca tcgataccgt cgacctcgag acctagaaaa acatggagca atcacaagta
     5761 gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg
     5821 tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag
     5881 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     5941 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga
     6001 actacacacc agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag
     6061 taccagttga gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac
     6121 accctgtgag cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg
     6181 acagccgcct agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc
     6241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     6301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     6361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc
     6421 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     6481 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     6541 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     6601 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     6661 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
     6721 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     6781 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     6841 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     6901 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     6961 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     7021 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     7081 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     7141 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     7201 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     7261 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     7321 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     7381 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
     7441 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
     7501 cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta
     7561 tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc
     7621 cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct
     7681 cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac
     7741 cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg
     7801 ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg
     7861 ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc
     7921 cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt
     7981 gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     8041 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     8101 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     8161 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     8221 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     8281 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     8341 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     8401 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     8461 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     8521 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     8581 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     8641 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     8701 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     8761 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     8821 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     8881 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     8941 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     9001 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     9061 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     9121 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     9181 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     9241 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     9301 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     9361 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     9421 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     9481 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     9541 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     9601 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     9661 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     9721 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     9781 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     9841 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     9901 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     9961 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    10021 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    10081 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    10141 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    10201 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    10261 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    10321 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    10381 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc
    10441 gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag
    10501 ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa
    10561 atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt
    10621 aggcgttttg cgctgcttcg
//
