LOCUS       sequence-415076-       11509 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11509)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11509)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2095
FEATURES             Location/Qualifiers
     source          1..11509
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    7366..7954
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7419..7439)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7837..7848)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8026..8259
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8337..8458
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8374..8393)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8428..8447
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8498..8633
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8653..8672)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8654..8672)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8682..8699)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8682..8698)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8691..8713)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8840..9295
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8927..8946)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9137..9158
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9321..9425
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9426..9494,9495..10286)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9644..9663)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10457..11045
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10946..10965
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11199..11216
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11333..11354
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11369..11374,11375..11392,11393..11399)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11407..11423
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11412..11434
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11431..11447
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11431..11447
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11466..11486
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11468..11486
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggat gcagaggctg aagataaaac
     4141 gctgcgtact cgctctaaag gaaccgaggt gccaatggat tcactaatcc aggagctcag
     4201 tgttgcctat gattgctcca tggcaaagaa gagaacagct gaagatcagg ctttgggggt
     4261 tccagtcaac aaaaggaaat ccctgctaat gaagccccga cactacagcc caaaagcaga
     4321 ctgccaagaa gaccgcagtg acaggacaga ggacgatggc cccttggaaa cacatggtca
     4381 ctctaccgca gaggaaatca tgataaaacc tatggatgaa agtcttcttt caactgcaca
     4441 agaaaactcc agtaggaagg aagacagata ctcttgttat caagagctca tggtcaagtc
     4501 tttaatgcac ttggggaaat ttgaaaaaaa tgtatctgtt cagactgtaa gtgaaaattt
     4561 aaatgacagt ggcatccagt ctttaaaagc agagagcgat gaagcagacg agtgctttct
     4621 gattcattct gatgatggaa gagacaagat tgatgattct cagccaccct tctgctcctc
     4681 tgatgacaat gaaagtaact ctgaaagtgc agaaaatggc tgggacagtg gctccaactt
     4741 ctcagaagaa accaaaccac ctagagtccc aaagtatgtt ttaacagatc ataaaaaaga
     4801 cctattggaa gttcctgaaa taaaaactga aggtgacaaa tttatccctt gtgagaacag
     4861 gtgtgattct gaaacagaaa ggaaagaccc gcagaatgct ctcgcagaac ccctggatgg
     4921 caatgcccag ccctcattcc ctgacgttga ggaggaagat agcgagagcc tggcagtaat
     4981 gacggaagag ggtagtgacc tggaaaaggc caaggggaat ttaagtttgc tggagcaggc
     5041 aattgctctg caggctgagc gaggttgtgt tttccataac acctacaaag agctggatag
     5101 gttcctgctg gagcacctag caggggaaag gaggcaaacc aaagttatcg acatgggtgg
     5161 aagacaaatc tttaacaata aacattcacc aaggcctgaa aagagggaga ccaagtgccc
     5221 gatccctgga tgtgatggca cgggacacgt gacagggctc tacccgcacc accgcagcct
     5281 ttcggggtgc ccccacaaag tgcgggttcc cctggaaatt cttgccatgc atgaaaatgt
     5341 gctcaagtgt cccacgccgg gatgcacagg aaggggtcat gtgaacagca accgcaacac
     5401 ccacaggagt ctttctggtt gtccaattgc tgcagctgaa aaattggcaa tgtcccagga
     5461 taaaaatcag cttgattctc cccaaactgg gcagtgtcct gaccaggccc acaggacaag
     5521 tttggtgaag caaattgaat tcaatttccc gtcacaagcc atcacctctc ccagagccac
     5581 agtgtcaaaa gaacaagaga agtttggaaa agtaccattt gattatgcca gttttgatgc
     5641 ccaagttttc ggtaaacgcc ctctcataca aacagtgcaa ggacgaaaaa caccaccatt
     5701 tcctgaatca aagcattttc caaatccagt gaaatttcct aatcgactgc ctagtgcagg
     5761 cgcccacacc cagagccctg gccgtgccag ctcttatagc tacggtcaat gtagtgaaga
     5821 cacccacata gcagcagctg ctgccatcct gaacctttcc acccgctgca gggaagccac
     5881 agacatcctc tccaacaagc cacagagtct gcatgccaag ggagccgaaa tagaagtgga
     5941 tgaaaatggc acattggact taagcatgaa aaaaaatcga atcctggaca agtctgcacc
     6001 cctaacttcc tctaacactt ctattccaac tccttcctct tccccattca aaacaagcag
     6061 cattctggtc aatgcagcat tctatcaggc tctttgtgac caagagggct gggacactcc
     6121 tatcaactat agcaaaactc acgggaagac agaggaggag aaagagaaag acccagtgag
     6181 ctctctagaa aatttagagg aaaaaaagtt tcctggagag gcctctatac caagccctaa
     6241 acccaagctt catgcaagag atctcaaaaa ggaactaatc acctgtccaa caccaggatg
     6301 tgatggaagt ggccacgtga caggaaacta tgcatctcat cgcagtgttt ctggatgtcc
     6361 tttagcagat aagactctaa aatccctcat ggctgccaac tctcaggagc ttaagtgtcc
     6421 aaccccaggc tgcgatggct cggggcacgt gactggaaac tatgcttccc acagaagctt
     6481 gtccggatgc cctcgtgcaa ggaaaggtgg tgtcaaaatg acccctacca aggaagaaaa
     6541 agaagaccct gaactgaaat gtcctgtgat agggtgtgat ggccaaggtc acatatcagg
     6601 taaatacaca tcacaccgca cagcttctgg ctgtcctctg gctgccaaga gacagaagga
     6661 gaatcctctc aatggagcct ccctctcctg gaaactgaac aaacaagagc taccacattg
     6721 tcccttgcca ggctgcaatg ggctgggcca tgtaaataat gtttttgtca cccaccgaag
     6781 cttatctgga tgtcctctca atgcacaagt tatcaaaaag ggcaaggttt ctgaagaact
     6841 catgaccatc aagctcaaag caactggggg aatagagagt gatgaagaaa ttaggcattt
     6901 ggatgaagaa ataaaggaac tgaatgaatc caaccttaaa attgaagcag atatgatgaa
     6961 acttcagacc cagatcacat ctatggagag caacttaaag acgatagagg aggagaacaa
     7021 actcatagaa cagaacaatg aaagtctgct gaaagagctg gcaggtctaa gccaagctct
     7081 catttcaagc cttgctgaca tccagcttcc acagatggga cctatcagtg agcagaattt
     7141 tgaagcatat gtaaatacac tcacagatat gtacagcaat ctggaacggg actattcccc
     7201 ggaatgcaaa gctctactgg aaagtatcaa acaggcagtg aagggtatcc atgtgtagta
     7261 atgaactagt ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa
     7321 ggacgaggta cgtctattac atctgttgag acgcgttaag tcgacaatca acctctggat
     7381 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     7441 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     7501 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     7561 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     7621 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     7681 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     7741 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     7801 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     7861 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     7921 acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac tttaagacca atgacttaca
     7981 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     8041 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     8101 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     8161 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     8221 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tacgtatagt agttcatgtc
     8281 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
     8341 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     8401 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     8461 atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     8521 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     8581 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac
     8641 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
     8701 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     8761 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     8821 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     8881 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     8941 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     9001 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     9061 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     9121 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     9181 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     9241 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
     9301 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     9361 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     9421 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     9481 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     9541 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     9601 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     9661 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     9721 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     9781 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     9841 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     9901 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     9961 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
    10021 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
    10081 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
    10141 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
    10201 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
    10261 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
    10321 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
    10381 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
    10441 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
    10501 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
    10561 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
    10621 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
    10681 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
    10741 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
    10801 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
    10861 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
    10921 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
    10981 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
    11041 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
    11101 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
    11161 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
    11221 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
    11281 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
    11341 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
    11401 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
    11461 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagc
//
