LOCUS       sequence-415651-        8423 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expression of codon-optimized mitochondrial base editor in human 
            cells; cell sorting; puromycin drug selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8423)
  TITLE     Quantifying constraint in the human mitochondrial genome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8423)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: m.5147-Right Puro DdCBE-G1397-C- T1413I-GFP
FEATURES             Location/Qualifiers
     source          1..8423
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1937..2185
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             2195..2251
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(2252..2254,2255..2257,2258..2971)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2252..2254,2255..2257,2258..2968)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2297..2318)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2558..2577)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2905..2926
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     rep_origin      3168..3596
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3255..3274)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3465..3486
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3605..3625)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3610..3939
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3790..3925
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3852..3871
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             3985..4584
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3985..4004)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4481..4501
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    4711..4832
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4748..4767)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4802..4821
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4881..4897)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4881..4897)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4894..4916)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4905..4921
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4929..4935,4936..4953,4954..4959))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4974..4995
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5112..5129)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5283..5871)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5363..5382)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6042..6833,6834..6902))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6665..6684
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6903..7007)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7082..7101)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7273..7652
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7653..7856
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7807..7827
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     7901..7920
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7901..7919
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             8035..8061
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             8065..8091
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             8095..8121
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tccgccgctg cagcttgata caggacaact gctgaagata gctaaacggg gaggtgtgac
       61 agctgtggag gctgtgcatg cctggcgaaa cgccctaacg ggcgcgccac tcaatctcac
      121 acccgagcaa gtggtggcca tcgctagcaa caacggcggc aagcaggctc tcgagacagt
      181 ccaagctctg ctgccagtgc tgtgccaagc tcacggcctg acccctgagc aggtggtggc
      241 tatcgcctct aatggcggag gcaagcaggc cctggaaacc gtgcagagac tgctgcctgt
      301 gctgtgccag gcccatggac tgacccctga gcaggtggtg gctatcgcct ctaatggcgg
      361 aggcaagcag gccctggaaa ccgtgcagag actgctgcct gtgctgtgcc aggcccatgg
      421 actgacccct gagcaggtgg tggctatcgc ctctaatggc ggaggcaagc aggccctgga
      481 aaccgtgcag agactgctgc ctgtgctgtg ccaggcccat ggactgacac ccgagcaggt
      541 ggtcgccatc gcctcccacg atggcggcaa acaggctctg gaaacagtgc aggccctgct
      601 gcctgtgctg tgccaggccc acggcctgac ccctcagcag gtggtcgcca tcgcctctaa
      661 catcggcgga aagcaggctc tggagaccgt gcagcggctg ctgcccgtgc tgtgccaggc
      721 ccacggcctc acacccgagc aagtggtggc catcgctagc aacaacggcg gcaagcaggc
      781 tctcgagaca gtccaagctc tgctgccagt gctgtgccaa gctcacggcc tcacacccga
      841 gcaagtggtg gccatcgcta gcaacaacgg cggcaagcag gctctcgaga cagtccaagc
      901 tctgctgcca gtgctgtgcc aagctcacgg cctgacccct gagcaggtgg tggctatcgc
      961 ctctaatggc ggaggcaagc aggccctgga aaccgtgcag agactgctgc ctgtgctgtg
     1021 ccaggcccat ggactcacac ccgagcaagt ggtggccatc gctagcaaca acggcggcaa
     1081 gcaggctctc gagacagtcc aagctctgct gccagtgctg tgccaagctc acggcctgac
     1141 acccgagcag gtggtcgcca tcgcctccca cgatggcggc aaacaggctc tggaaacagt
     1201 gcaggccctg ctgcctgtgc tgtgccaggc ccacggcctc acacccgagc aagtggtggc
     1261 catcgctagc aacaacggcg gcaagcaggc tctcgagaca gtccaagctc tgctgccagt
     1321 gctgtgccaa gctcacggcc tgacccctca gcaggtggtc gccatcgcct ctaacatcgg
     1381 cggaaagcag gctctggaga ccgtgcagcg gctgctgccc gtgctgtgcc aggcccacgg
     1441 cctcacaccc gagcaagtgg tggccatcgc tagcaacaac ggcggcaagc aggctctcga
     1501 gacagtccaa gctctgctgc cagtgctgtg ccaagctcac ggcctgaccc ctcagcaggt
     1561 ggtcgccatc gcctctaaca tcggcggaaa gcaggctctg gagaccgtgc agcggctgct
     1621 gcccgtgctg tgccaggccc acggcctgac cccccagcag gtggtggcca tcgcctccaa
     1681 tggcggcggg agacccgccc tggaatccat agtagcacag ttgtctaggc cagaccctgc
     1741 cctggccgcc ctgaccaatg atcacctggt ggcgctggct tgcctgggcg gtcgtccggc
     1801 cctggatgcg gtgaagaaag gcctgggtgg atccgccatt ccagtgaagc gcggcgctac
     1861 cggtgaaacc aaagtgttta tcggtaacag caacagcccg aagagcccga ccaaaggcgg
     1921 ttgctctggc ggctcaacta atctgagcga catcattgag aaggagactg ggaaacagct
     1981 ggtcattcag gagtccatcc tgatgctgcc tgaggaggtg gaggaagtga tcggcaacaa
     2041 gccagagtct gacatcctgg tgcacaccgc ctacgacgag tccacagatg agaatgtgat
     2101 gctgctgacc tctgacgccc ccgagtataa gccttgggcc ctggtcatcc aggattctaa
     2161 cggcgagaat aagatcaaga tgctgggaag cggagctact aacttcagcc tgctgaagca
     2221 ggctggagac gtggaggaga accctggacc tatggtgagc aagggcgagg agctgttcac
     2281 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     2341 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     2401 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct atggagtgca
     2461 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     2521 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     2581 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     2641 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     2701 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
     2761 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     2821 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa
     2881 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     2941 cactctcggc atggacgagc tgtacaagtg ataaggggtc tttgtcctct gtactgtctc
     3001 tctccttgcc cctaacccaa aaagcttcat ttttctgtgt aggctgcaca agagccttga
     3061 ttgaagatat attctttctg aacagtattt aaggtttcca ataaaatgta cacccctcag
     3121 aacttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
     3181 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     3241 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     3301 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     3361 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     3421 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     3481 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     3541 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
     3601 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     3661 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     3721 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     3781 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     3841 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
     3901 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
     3961 tccattttcg gatctgatca gcacatgacc gagtacaagc ccacggtgcg cctcgccacc
     4021 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     4081 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     4141 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
     4201 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     4261 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     4321 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg agtctcgccc
     4381 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     4441 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     4501 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     4561 atgacccgca agcccggtgc ctgagtgcta cgagatttcg attccaccgc cgccttctat
     4621 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     4681 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
     4741 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     4801 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
     4861 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     4921 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     4981 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     5041 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     5101 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     5161 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     5221 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     5281 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     5341 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     5401 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     5461 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     5521 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     5581 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     5641 ggtaacagga ttagcagagc gaggtatata ggcggtgcta cagagttctt gaagtggtgg
     5701 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     5761 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     5821 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     5881 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     5941 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     6001 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     6061 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     6121 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     6181 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     6241 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     6301 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     6361 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     6421 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     6481 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     6541 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     6601 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     6661 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     6721 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     6781 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     6841 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     6901 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     6961 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     7021 aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg tgcactctca
     7081 gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg
     7141 aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca
     7201 attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca
     7261 gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
     7321 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
     7381 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
     7441 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
     7501 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
     7561 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     7621 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     7681 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     7741 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     7801 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctctct
     7861 ggctaactag agaacccact gcttactggc ttatcgaaat taatacgact cactataggg
     7921 agacccaagc tggctagcac catggccctg tcccgtgcgg tttgtggcac ctcccgtcaa
     7981 ctggctccgg ttctgggtta tctgggttcc cgtcaaaaac actccctgcc ggactacccg
     8041 tatgatgttc cggattacgc tggctaccca tacgacgtcc cagactacgc tggctaccca
     8101 tacgacgtcc cagactacgc tatggacatc gcggatctgc gtaccctggg ttacagccag
     8161 cagcagcagg agaagatcaa gccgaaggtg cgcagcaccg tggctcagca ccacgaagcc
     8221 ctggtgggcc acggtttcac ccacgctcac attgtggccc tgagccagca cccagccgcg
     8281 ctgggcaccg tggccgtgaa atatcaggat atgattgctg ccctgccaga ggccacccat
     8341 gaagctattg tgggcgtggg caagcagtgg agcggtgctc gtgcgctgga ggcgctgctg
     8401 accgtggctg gtgaactgcg tgg
//
