LOCUS       sequence-415708-       11988 bp    DNA     circular SYN 14-APR-2025
DEFINITION  expression of rabbit Cav2.2 calcium channel with an exofacial double
            HA tag in domain II and a D122A mutation that disrupts the 
            interaction with ?2?-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11988)
  AUTHORS   Dahimene S, Page KM, Kadurin I, Ferron L, Ho DY, Powell GT, Pratt 
            WS, Wilson SW, Dolphin AC
  TITLE     The alpha(2)delta-like Protein Cachd1 Increases N-type Calcium 
            Currents and Cell Surface Expression and Competes with 
            alpha(2)delta-1.
  JOURNAL   Cell Rep. 2018 Nov 6;25(6):1610-1621.e5. doi: 
            10.1016/j.celrep.2018.10.033.
  PUBMED    30404013
REFERENCE   2  (bases 1 to 11988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2018.10"; journalName: "Cell Rep"; date: 
            "2018-11-6- 6"; volume: "25"; issue: "6"; pages: "1610-1621.e5"
COMMENT     Sequence Label: Cav2.2 D122A HA pCAGGS
FEATURES             Location/Qualifiers
     source          1..11988
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     131..150
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(369..473)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        504..883
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        885..1162
                     /label=chicken beta-actin promoter
     intron          1163..2179
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2103..2125
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2187..2206
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             3973..3999
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4003..4029
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(9509..9528)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    9574..9629
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(9628..9647)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(9990..10006)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9990..10006)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10003..10025)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10014..10030
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10038..10044,10045..10062,10063..10068))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10083..10104
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        10163..10358
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      10209..10344
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    10364..10498
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10401..10420)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10455..10474
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10566..10583)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10737..11325)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10817..10836)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11496..11988,1..299,300..368))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
       61 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
      121 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
      181 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
      241 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
      301 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
      361 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
      421 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
      481 ccccgaaaag tgccacctgg gtcgacattg attattgact agttattaat agtaatcaat
      541 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
      601 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
      661 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
      721 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
      781 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
      841 tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac
      901 gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat
      961 tttttaatta ttttgtgcag cgatgggggc gggggggggg ggggggcgcg cgccaggcgg
     1021 ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca
     1081 gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa
     1141 aaagcgaagc gcgcggcggg cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc
     1201 cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag
     1261 cgggcgggac ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt
     1321 ttcttttctg tggctgcgtg aaagccttga ggggctccgg gagggccctt tgtgcggggg
     1381 gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc
     1441 gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt
     1501 gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa
     1561 caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt
     1621 cgggctgcaa ccccccctgc acccccctcc ccgagttgct gagcacggcc cggcttcggg
     1681 tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca
     1741 ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg gagggctcgg gggaggggcg
     1801 cggcggcccc cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt
     1861 atggtaatcg tgcgagaggg cgcagggact tcctttgtcc caaatctgtg cggagccgaa
     1921 atctgggagg cgccgccgca ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg
     1981 caggaaggaa atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc
     2041 tctccagcct cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc
     2101 ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc
     2161 cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt
     2221 tggcaaagaa ttcctcgagg gtaccgggcc ccccctcgag gtcgaagctt gatcatggtc
     2281 cgcttcgggg acgagctggg cggccgctat gggggcgcgg gcggcgcgga gcgggcccgg
     2341 ggcggcgggg ccggcggggc tggcggcccg ggccccgggg ggctgccgcc gggccagcgg
     2401 gtcctctaca aacagtcgat cgcgcagcgc gcgcggacca tggcgctgta caaccccatc
     2461 ccggtcaagc agaactgctt caccgtcaac cgctcgctct tcgtcttcag cgaggacaac
     2521 gtcgtccgca aatacgccaa gcgcatcacc gagtggcctc cgttcgagta catgatcctg
     2581 gccaccatca tcgccaactg catcgtgctg gccctggagc agcacctccc tgacggggcc
     2641 aagacgccta tgtccgagcg cctggatgac accgagccct acttcattgg gatcttctgc
     2701 ttcgaggcgg ggatcaagat cctggccctg ggctttgttc tgcacaaggg ctcctatctg
     2761 cggaacggct ggaacgtcat ggacttcgtg gtagtcctca cggggattct tgccacagcc
     2821 ggaaccgact tcgacctgcg aacgctgagg gctgtgcgtg tgctaaggcc cctgaagctg
     2881 gtgtctggga ttccgagctt gcaagtggtg ctcaagtcca tcatgaaggc catggtcccg
     2941 ctgctgcaga tcgggctgct gctcttcttc gccatcctca tgtttgccat catcggcctc
     3001 gagttctaca tgggcaagtt ccacaaggcc tgcttcccca acagcacaga cccagatccc
     3061 gtgggtgact tcccctgtgg caaggaggcc ccggcccggc tctgtgaggg agacacggag
     3121 tgccgggagt actgggccgg acccaacttt ggtatcacca actttgacaa catcctgttt
     3181 gccatcttga ccgtgttcca gtgcatcacc atggagggct ggactgacat cctctacaat
     3241 acaaacgatg cagccggcaa cacgtggaac tggctctact tcatccctct catcatcatc
     3301 ggctccttct tcatgctcaa cctggtgctg ggtgtgctct caggagagtt tgctaaggag
     3361 cgcgagagag tggagaaccg ccgcgccttc ctgaagctcc ggcggcagca gcagattgag
     3421 cgggagctca acgggtacct ggagtggatc ttcaaggctg aggaagtcat gctggctgag
     3481 gaggacagga acgcagagga gaagtcccct ctggatgcag tgttgaagag agcagctgcc
     3541 aagaagagca gaagcgacct gatccaggcc gaggaggggg agggccggct cacaggcctc
     3601 tgtgctcccg gttccccgtt cgcccgtgcc agcctgaaaa gcgggaagac ggagagctcg
     3661 tcgtacttcc ggaggaagga gaagatgttc cgcttcttca tccggcgcat ggtgaaggca
     3721 cagagcttct actggacggt gctgtgtgtg gtggcactga acacactgtg cgtggccatg
     3781 gtgcactaca accagccgca gcggctcacc acggcattgt actttgcaga gtttgttttc
     3841 ctgggtctct tcctcacaga gatgtccctg aagatgtatg gcctggggcc caggagctac
     3901 ttccggtcct ccttcaactg ttttgacttt ggggtcatcg tgggcagtat ctttgaagtg
     3961 gtctgggctg cttaccccta cgacgtcccc gactacgccg gctacccgta cgacgtcccg
     4021 gactacgccg gcgtcgacgt caagccaggg acctccttcg ggatcagtgt tctgcgggct
     4081 ctgcgcttac tgagaatctt caaggtcacc aaatactgga actccctacg gaacctggtg
     4141 gtgtctctgc tcaactccat gaagtccatc atcagcctcc tcttcctgct cttcctgttc
     4201 attgttgtct ttgctctgct ggggatgcag ctgtttggag gccagttcaa ctttaaagac
     4261 gagaccccaa cgaccaactt tgacaccttc cctgctgcta tcctcaccgt cttccagatc
     4321 ctgaccggag aggactggaa tgcggtcatg taccacggga tcgagtcgca aggtggcgtc
     4381 agcagaggaa tgttctcctc cttctacttc attgtcctca ctctgtttgg gaactacacc
     4441 ctgctgaatg tcttcctggc cattgctgtg gacaaccttg ccaatgctca ggagctgacc
     4501 aaggatgaag aggagatgga agaagcagcc aatcagaaac tcgccttgca gaaggccaag
     4561 gaagtggctg aagtcagccc catgtctgct gcgaacatct ccattgccgc caggcagcag
     4621 aactcggcca aagcgcgctc ggtgtgggag cagcgagcca gccagctgcg gctgcagaac
     4681 ctgagggcca gctgcgaggc gctgtacagc gagatggacc ccgaggagag gctgcgctac
     4741 gccaccgcgc gccacctgcg gcccgacgtg aagacgcacc tggaccggcc gctggtggtt
     4801 gagcccggcc gcgacgctcc gcggggcccc ccggggggga agtcccggcc ggatggctcg
     4861 gaggcccccg agggtgcgga tcccccgcgc aggcatcacc ggcaccgaga caaggacaag
     4921 gctcccgcca cggtcccctc ggcgggggaa caggacagag ccgaagctct gagggcagag
     4981 ggcggggagc tggggccccg cgaggagcgg gggcggccac gccgcagccg cagcaaggag
     5041 gccccgggcg ccccggaggt gcgcagcgat cgcggccgtg gcccgtgtcc tgaaggtggt
     5101 cggcgccacc accggcgcgg gtcaccggag gaggcagctg agagggagcc ccgccgccac
     5161 cgcgcgcacc ggcacgggcc cgacccgggc aaggagggcc cagcgtccgg gaccaggggc
     5221 gagcggcgcg cacgacaccg cacgggcccc cgggcgtgcc cccgggaggc tgagagcagc
     5281 gaggagcctg cgcggcggca ccgggctcgg cacaaggcgc cgcccaccca ggagaccgcg
     5341 gagaaggaca aggaggctgc cgagaagggg ggcgaggcca cggaggccga gaaggacaag
     5401 gaggctcgga accaccagcc caaggagctg ccctgtgacc tggaggccat cggcatgctg
     5461 ggagtgggcg ctgtgcatac gctgcccagc acctgtctgc agaaggtgga ggagcagcca
     5521 gaggatgcgg acaaccagcg gaacgtcacc cgcatgggta gtcagccccc ggacaccagc
     5581 accactgtgc acattcctgt gacgctgacc ggcccccccg gggagaccac ggtagtgccc
     5641 agtggaaacg tggacctgga aagtcaagca gaggggaaga aggaggtgga aaccagcgat
     5701 gtgatgagga gcggccctcg gcccattgtc ccgtacagtt ccatgttctg tttaagccca
     5761 accaacctgc tgcgccgctg ctgccactac attgtgacca tgcgatactt cgagatggtc
     5821 atcctcgtgg tcatcgccct gagcagcatc gccctggctg ccgaggaccc cgtgagaaca
     5881 gactctccca ggaataatgc tctgaagtac atggactaca tcttcacagg cgtcttcacc
     5941 ttcgagatgg tgataaagat gatcgatttg ggactgctgc tacaccctgg agcctatttc
     6001 cgggatctgt ggaacattct ggacttcatt gtggtcagtg gggccctggt ggcatttgct
     6061 ttctcaggat ccaaagggaa agacatcagc accatcaaat ccctgagagt cctgcgtgtg
     6121 ctgcggccac tcaagaccat caagcggctg cccaagctca aggccgtgtt tgactgtgtg
     6181 gtgaactcac tgaaaaacgt cctcaacatc ctgatcgtct acatgctctt catgttcatc
     6241 tttgccgtca ttgccgtgca gctcttcaaa gggaagttct tctactgcac ggacgaatcc
     6301 aaggagctgg agcgtgactg caggggtcag tatttggatt atgagaagga ggaagtggaa
     6361 gctcaaccga ggcagtggaa gaaatatgac ttccactatg acaacgtgct ctgggctctg
     6421 ctaacgctct tcacggtgtc cacaggggaa gggtggccca tggtgctgaa acactctgtg
     6481 gatgccacct acgaggagca gggcccgagc cccgggtacc gcatggagct ctccatcttc
     6541 tacgtggtct actttgtggt ctttcccttc ttctttgtca acatcttcgt ggccttgatc
     6601 atcatcacct tccaggagca gggggacaag gtgatgtccg agtgcagcct ggagaagaac
     6661 gagagggctt gcatcgactt tgccatcagc gccagacccc tgacgcggta catgccccag
     6721 aacaagcagt cgttccagta taagacgtgg acgttcgtgg tgtccccacc ttttgagtac
     6781 ttcatcatgg ctatgatcgc cctcaacact gtggtgctga tgatgaagtt ctacgatgcg
     6841 ccgtacgagt atgagctaat gctgaaatgc ctgaacatcg tgttcacgtc catgttctcc
     6901 atggagtgtg tgctgaagat catcgccttc ggggtgctga actatttcag agatgcctgg
     6961 aatgtctttg actttgtcac ggtgttggga agtattactg atattttggt aacagagatt
     7021 gcgaacaact tcatcaacct cagcttcctg cgcctgttcc gggccgcgcg gctcatcaag
     7081 ctgctgcgcc agggctacac catccgcatc ctgctttgga ccttcgtgca gtccttcaag
     7141 gcgctgccct acgtgtgtct cctcatcgcc atgctcttct tcatctacgc catcattggc
     7201 atgcaggttt ttgggaacat cgccctggac gatgacacca gcatcaacag gcacaataac
     7261 ttccggacgt ttctgcaggc cttgatgttg ctgttcagga gcgccacggg ggaggcctgg
     7321 cacgagataa tgctgtcgtg cctcagcagc cgggcctgcg acgagcactc caatgccagc
     7381 gagtgcggca gcgactttgc ctacttttac ttcgtctcct tcatcttcct ctgctccttt
     7441 ctgatgttga acctctttgt ggctgtgatc atggacaatt ttgagtacct tacacgggac
     7501 tcttccatct tagggcctca ccacctggat gagttcatcc gggtctgggc tgaatacgac
     7561 ccggccgcgt gtgggcgcat cagttacagt gacatgtttg agatgctgaa gcacatgtcc
     7621 ccacccctgg ggctggggaa gaagtgccct gctcgagtcg cgtacaagcg cctggtccgc
     7681 atgaacatgc ccatctccag tgaggacatg acggtgcact tcacatccac gctgatggct
     7741 ctcattcgga cagcgctgga catcaagctg gcaccagctg ggaccaagca gcaccagtgc
     7801 gacgcagagc tgaggaagga gatttcctgt gtgtgggcca atctgcccca gaagaccctg
     7861 gacttactgg taccacccca taagcctgac gagatgacgg tgggcaaagt ctatgcagct
     7921 ctgatgatct tcgacttcta caaacagaac aaaaccagca gagaccagac tcagcaagcc
     7981 cccggaggcc tgtcccagct gggccccgtg tccctgttcc acccgctgaa ggccacgctg
     8041 gagcagactc agccggcgct gcgtggagcc cgggctttcc tgcggcagaa gagttctgcc
     8101 tccctgagca acggcggggc cgtacaaacc caggagagcg gcatcaagga atcggtttct
     8161 tggggcactc agaggaccca ggatgtgctt tgcgaggcca gggcacccct ggagcggggc
     8221 cactcagctg agatcccagt cgggcagcct gggacactgg ctgtagatgt ccagatgcag
     8281 aacatgactc tgagtggccc tgacgccgag ccgcagccag ggctggagag ccagggccga
     8341 gccgcctcca tgccccgcct ggccgcagaa acacagcccg cgcctgacgc cagccccatg
     8401 aagcgctcca tctccacgct ggccccgcgc ccccacacgg cccggctcgg cagcaccgcg
     8461 ctggaccgcc cagcccccag ccaggccccg caccatcacc accaccgctg ccaccgccgc
     8521 agggacagga agcagcggtc cctggagaag ggtcccagcc tgtctgctga cacagatggg
     8581 gcccccgaca gcacggtggg gccaggactg cccacggggg agggcccccc cggctgcagg
     8641 cgggagcggg agcgcaggca ggagcggggc cggtcccagg agcggaggca gccctcgtcc
     8701 tcctcctcgg agaagcatcg cttctactcc tgtgaccgct tcgggggccg cgaaccccca
     8761 cagcccaagc cctccctcag tagtcacccc acgtcgccga cggccgggca ggagccggga
     8821 ccccacccgc aggggagtgg ttcggttcac gggagccccc tgctgtccac atctggtgct
     8881 agcaccccgg gccgggggcg gaggcagctc ccccagaccc ccttgacccc ccgccccagc
     8941 gtcacctaca agacggccaa ctcctcgcct gtccacttcg ccggggcacc gagcggcctc
     9001 cccgccttct cccccggccg gctcagccgg ggcctctcag aacacaacgc cctgctgcag
     9061 agggacccgc tcagccggcc cctggcgccc ggctcgcgca tcggctccga cccctacctg
     9121 gggcagcggc tggacagcga ggcgcccgcc cgtgccctgc ccgaggacgc gcccgccttt
     9181 gaggagaccg cggccagcaa ctcgggccgc tcctcccgga cctcgtacgt gtcctcactg
     9241 acctcgcagc ccccgccgct gcgccgcgtg cccaacggct accactgcac tctggggctg
     9301 ggcgggggcg gccgggcgcg gcgcggctgc caccaccccg accgcgaccg ccggtgctag
     9361 actagttcta gcaagcttcg acgaattcgc atgcggccgc tagctcgagg taccgatatc
     9421 gagctcaatt cactcctcag gtgcaggctg cctatcagaa ggtggtggct ggtgtggcca
     9481 atgccctggc tcacaaatac cactgagatc tttttccctc tgccaaaaat tatggggaca
     9541 tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt ttcattgcaa
     9601 tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt
     9661 taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgcccat atgctggctg
     9721 ccatgaacaa aggttggcta taaagaggtc atcagtatat gaaacagccc cctgctgtcc
     9781 attccttatt ccatagaaaa gccttgactt gaggttagat tttttttata ttttgttttg
     9841 tgttattttt ttctttaaca tccctaaaat tttccttaca tgttttacta gccagatttt
     9901 tcctcctctc ctgactactc ccagtcatag ctgtccctct tctcttatgg agatccctcg
     9961 acctgcagcc caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
    10021 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
    10081 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
    10141 aacctgtcgt gccagcggat ccgcatctca attagtcagc aaccatagtc ccgcccctaa
    10201 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
    10261 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
    10321 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctaacttgt ttattgcagc
    10381 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
    10441 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatccg
    10501 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
    10561 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
    10621 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
    10681 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
    10741 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
    10801 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
    10861 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
    10921 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
    10981 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
    11041 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
    11101 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
    11161 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
    11221 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
    11281 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
    11341 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
    11401 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
    11461 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
    11521 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
    11581 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
    11641 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
    11701 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
    11761 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
    11821 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
    11881 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
    11941 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggca
//
