LOCUS       sequence-41581-e         677 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  used in the derivation of human iPS cells using non-integrating 
            episomal vectors; expresses Oct4 and Sox2; SV40LT and Klf4 .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Yu J, Hu K, Smuga-Otto K, Tian S, Stewart R, Slukvin II, Thomson JA
  TITLE     Human Induced Pluripotent Stem Cells Free of Vector and Transgene 
            Sequences
  JOURNAL   Science. 2009 Mar 26.
  PUBMED    19325077
REFERENCE   2  (bases 1 to 677)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2009 Mar 26."
COMMENT     Sequence Label: pEP4 E02S ET2K
FEATURES             Location/Qualifiers
     source          1..677
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(576..595)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 ggcagcgtgt acttatcctt cttcatgagc gtcttggttt tccgccgggg ccggtattta
       61 taatccgggt gctccttcat gtgcagcgct cgcagccgct tagcctcgtc gatgaacggc
      121 cgcttctccg tctccgacaa aagtttccac tcggcgccca ggcgcttgct gatctccgag
      181 ttgtgcatct tggggttctc ctgggccatc ttgcgccgct gcccgcggga ccacaccatg
      241 aaggcattca tgggccgctt gacgcggtcc gggctgtttt tctggttgcc gccggccgcc
      301 gccgcggtgg agttgccgcc gccgcccccc gaagtttgct gcgggcccgg cggcttcagc
      361 tccgtctcca tcatgttgta catgcggaat cgaattaatt cgcggtgtgg ccatattatc
      421 atcgtgtttt tcaaaggaaa accacgtccc cgtggttcgg ggggcctaga cgttttttta
      481 acctcgacta aacacatgta aagcatgtgt accgaggccc cagatcagat cccatacaat
      541 ggggtacctt ctgggcatcc ttcagcccct tgntgaatac gcttgaggag agccatttga
      601 ctctttccac aactatccaa ctcacaacgt ggcactgggg ttgtgccgcc tttgcaggtg
      661 tatcttatac acgtggc
//
