LOCUS       sequence-415861-       13387 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13387)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 13387)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0253
FEATURES             Location/Qualifiers
     source          1..13387
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     497..516
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(548..744)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        complement(556..766)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      562..697
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             complement(997..1041)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1226..1459
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1952..2077
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             2124..2304
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(2305..2531)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(2559..2579)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2559..2577)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2598..2614)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2598..2614)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2611..2633)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2622..2638
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2646..2652,2653..2670,2671..2676))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2691..2712
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2829..2846)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3000..3588)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3080..3099)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3759..4550,4551..4619))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4382..4401
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4620..4724)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4750..5205)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4887..4908)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5099..5118
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5332..5354
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5347..5363
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5373..5392
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5373..5391
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      5412..5547
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5587..5708
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5598..5617)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5652..5671
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             5786..6019
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    6091..6679
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             6197..6208
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     6606..6626
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     promoter        complement(11825..13003)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          11834..12772
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(11857..11877)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    13141..13258
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(join(13301..13387,1..516))
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(join(13387,1..20))
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 ctcgtagaag gggaggttgc ggggcgcgga ggtctccagg aaggcgggca ccccggcgcg
       61 ctcggccgcc tccactccgg ggagcacgac ggcgctgccc agacccttgc cctggtggtc
      121 gggcgagacg ccgacggtgg ccaggaacca cgcgggctcc ttgggccggt gcggcgccag
      181 gaggccttcc atctgttgct gcgcggccag ccgggaaccg ctcaactcgg ccatgcgcgg
      241 gccgatctcg gcgaacaccg cccccgcttc gacgctctcc ggcgtggtcc agaccgccac
      301 ggcggcgccg tcgtccgcga cccacacctt gccgatgtcg agcccgacgc gcgtgaggaa
      361 gagttcttgc agctcggtga cccgctcgat gtggcggtcc ggatcgacgg tgtggcgcgt
      421 ggcggggtag tcggcgaacg cggcggcgag ggtgcgtacg gccctgggga cgtcgtcgcg
      481 ggtggcgagg cgcaccgtgg gcttgtactc ggtcatggta agctccggtg gtacctctag
      541 aaagcttttt gcaaaagcct aggcctccaa aaaagcctcc tcactacttc tggaatagct
      601 cagaggccga ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg
      661 gagaatgggc ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggacta
      721 tggttgctga ctaattgaga cgcatgcttt gcatacttct gcctgctggg gagcctgggg
      781 actttccaca cctggttgct gactaattga gatgcaatgc atgggtcccc tcggggttgg
      841 gaggtgggtc tgaaacgata atggtgaata tccctgccta actctattca ctatagaaag
      901 tacagcaaaa actattctta aacctaccaa gcctcctact atcattatga ataattttat
      961 ataccacagc caatttgtta tgttaaacca attccacaaa cttgcccatt tatctaattc
     1021 caataattct tgttcattct tttcttgctg gttttgcgat tcttcaatta aggagtgtat
     1081 taagcttgtg taattgttaa tttctctgtc ccactccatc caggtcgtgt gattccaaat
     1141 ctgttccaga gatttattac tccaactagc attccaaggc acagcagtgg tgcaaatgag
     1201 ttttccagag caaccccaaa tccccaggag ctgttgatcc tttaggtatc tttccacagc
     1261 caggattctt gcctggagct gcttgatgcc ccagactgtg agttgcaaca gatgctgttg
     1321 cgcctcaata gccctcagca aattgttctg ctgctgcact ataccagaca ataattgtct
     1381 ggcctgtacc gtcagcgtca ttgacgctgc gcccatagtg cttcctgctg ctcccaagaa
     1441 cccaaggaac aaagctccta ttcccactgc tcttttttct ctctgcacca ctcttctctt
     1501 tgccttggtg ggtgctactc ctaatggttc aatttttact actttatatt tatataattc
     1561 acttctccaa ttgtccctca tatctcctcc tccaggtctg aagatcagcg gccgcttgct
     1621 gtgcggtggt cttacttttg ttttgctctt cctctatctt gtctaaagct tccttggtgt
     1681 cttttatctc tatcctttga tgcacacaat agagggttgc tactgtatta tataatgatc
     1741 taagttcttc tgatcctgtc tgaagggatg gttgtagctg tcccagtatt tgtctacagc
     1801 cttctgatgt ttctaacagg ccaggattaa ctgcgaatcg ttctagctcc ctgcttgccc
     1861 atactatatg ttttaattta tattttttct ttccccctgg ccttaaccga attttttccc
     1921 atcgcgatct aattctcccc cgcttaatac tgacgctctc gcacccatct ctctccttct
     1981 agcctccgct agtcaaaatt tttggcgtac tcaccagtcg ccgcccctcg cctcttgccg
     2041 tgcgcgcttc agcaagccga gtcctgcgtc gagagagctc ctctggtttc cctttcgctt
     2101 tcaagtccct gttcgggcgc cactgctaga gattttccac actgactaaa agggtctgag
     2161 ggatctctag ttaccagagt cacacaacag acgggcacac actacttgaa gcactcaagg
     2221 caagctttat tgaggcttaa gcagtgggtt ccctagttag ccagagagct cccaggctca
     2281 gatctggtct aaccagagag acccgtttat gtatcgagct aggcacttaa atacaatatc
     2341 tctgcaatgc ggcaattcag tggttcgtcc aatccatgtc agacccgtct gttgccttcc
     2401 taataaggca cgatcgtacc accttacttc caccaatcgg catgcacggt gctttttctc
     2461 tccttgtaag gcatgttgct aactcatcgt taccatgttg caagactaca agagtattgc
     2521 ataagactac attaagcttg cagctccagc ttttgttccc tttagtgagg gttaattgcg
     2581 cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     2641 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     2701 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     2761 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     2821 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     2881 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     2941 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     3001 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     3061 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     3121 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     3181 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     3241 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     3301 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     3361 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     3421 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     3481 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     3541 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     3601 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     3661 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     3721 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     3781 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     3841 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     3901 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     3961 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     4021 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     4081 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     4141 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     4201 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     4261 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     4321 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     4381 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     4441 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     4501 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     4561 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     4621 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     4681 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     4741 gtgccaccta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
     4801 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
     4861 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
     4921 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
     4981 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
     5041 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
     5101 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
     5161 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat tcgccattca
     5221 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
     5281 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
     5341 gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga ctcactatag ggcgaattgg
     5401 gtacgtccct aggcctccaa aaaagcctcc tcactacttc tggaatagct cagaggccga
     5461 ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg gagaatgggc
     5521 ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggatag ctagagccag
     5581 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
     5641 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
     5701 aacaagttcc tctcactctc tgatattcat ttctttgcaa gttataaata ctgaataata
     5761 agatgacatg aactactata cgtactgcta gagattttcc acactgacta aaagggtctg
     5821 agggatctct agttaccaga gtcacacaac agacgggcac acactacttg aagcactcaa
     5881 ggcaagcttt attgaggctt aagcagtggg ttccctagtt agccagagag ctcccaggct
     5941 cagatctggt ctaaccagag agacccagta caagcaaaaa gcagatcttg tcttcgttgg
     6001 gagtgaatta gcccttccag tccccccttt tcttttaaaa agtggctaag atctacagct
     6061 gccttgtaag tcattggtct taaagtcgac gcggggaggc ggcccaaagg gagatccgac
     6121 tcgtctgagg gcgaaggcga agacgcggaa gaggccgcag agccggcagc aggccgcggg
     6181 aaggaaggtc cgctggattg agggccgaag ggacgtagca gaaggacgtc ccgcgcagaa
     6241 tccaggtggc aacacaggcg agcagccaag gaaaggacga tgatttcccc gacaacacca
     6301 cggaattgtc agtgcccaac agccgagccc ctgtccagca gcgggcaagg caggcggcga
     6361 tgagttccgc cgtggcaata gggaggggga aagcgaaagt cccggaaagg agctgacagg
     6421 tggtggcaat gccccaacca gtgggggttg cgtcagcaaa cacagtgcac accacgccac
     6481 gttgcctgac aacgggccac aactcctcat aaagagacag caaccaggat ttatacaagg
     6541 aggagaaaat gaaagccata cgggaagcaa tagcatgata caaaggcatt aaagcagcgt
     6601 atccacatag cgtaaaagga gcaacatagt taagaatacc agtcaatctt tcacaaattt
     6661 tgtaatccag aggttgattg tcgacttaac gcgtattagg cgggcggccc ttgacaggtc
     6721 gtcctttcca caagatatat aaagccaaga aatcgaaata ctttcaagtt acggactagt
     6781 tcattactaa gttccttggc ctttttcaat tctttcactc cttttgtaca gcctatggtc
     6841 caccaggggg ctgtggcgac agcggcgaag aaacctcatt atttttcgag cagcttgatc
     6901 ctgctttttg gttagcaaac tgctcctgag tttctgttgt acaatcacag ccgtccggcg
     6961 agcctgccgt cttttgccac atttcttata acttcggtag tacttttgga tgagcacagc
     7021 agcccgtcgg ctctgctgga attttttttg ttcatagtaa cttcggaatt tgctctggat
     7081 aaggatggca gcctgtgtca tctttttata aagtgcgtac tgtttatatt ttctgtaaca
     7141 acgctgaata acagcagcag ctacttcttg ctgttcccgc aagggtcggc ccttgtattt
     7201 ccggaaagct gtctggacaa gcctggcagc ctcatagagt tctctctgtt catgatcaga
     7261 cagagtgagc tgagcaaact cattctctac cttctcactg gtagatgcac tcaggaattc
     7321 tgaccaatcc gcggcggagg gaaggttagg tttctcgaaa gcgatttcac tgacgggaga
     7381 gccaacaggg ctcaagctgg tattagaaga aggggttaga ggctcgttat atgcacttcg
     7441 gatctgggca gcactgggaa ggcagtcagc atccgctaga taactggcca gccagctcat
     7501 tgtactgcta atggtggcag ggtctgttct ttccaatcct gaggactcca tgggcacaaa
     7561 attctcctgc ttgattcggt caggtgtggc ttcaataatg tgttctgcca aggtcatcat
     7621 gttcacctgt atgtcatcca tgggctggcc gcattcttca ttttctgcac tatcacggta
     7681 ggaccccagc tctgtattca ccacctctct gttagccatc atcaggacac tcattggttc
     7741 ccgtggacgc acctgtgaag gtgctgcctc ctttcctaca ctggtcccct tcggatttcc
     7801 agtcacctgt actgtagaca caccaatgta aagatctttg gaattccact ttcctacagg
     7861 ctgagatcca gaggcttgaa atgctgcagt ctctggagtg ggaggggaga gttcccggct
     7921 gaagtccctc actccttcac tggggctgag tgtctcgggg acagactgct tagaactagg
     7981 gctttgcttc ctgatatttg gctcttccag actcagtgca gtgggaagca gcttctcctg
     8041 ccttgtctgg aagtactcag ggttcaattt atgctttttg ggagccggat aatctttgtg
     8101 gctctcactg ctgtagtaat tagagggttc agaacgaggt cttctcagct ctgggttggt
     8161 gcttgcaata acagtgactc ccttgggtat ttctggagag ctgatggctt cgctgtgcca
     8221 ctgggccatc cagctctctg tgctgggctc ttcgcttgca ggacagtgga ttctggggtt
     8281 ctgtcccagc tgagcctgct catctctctg caggtgctcc agacactctg ctaatttcac
     8341 atgaccccgt gacctggcaa ttcccaaagg cagccttcct agagagtcgg gaatcgagat
     8401 ggcccgacgg tcccacttgt acagcacgac ggcagcttcc aagtgcccta gggcacacgc
     8461 ccacatcaga ggagtacagg agaagtggtc cacattcaag gggtcaactt ccagttccag
     8521 gtcaatgcta tccgcgtgct ttgtacgcca tttgatgagg gtctggatta gggtggcata
     8581 gccctgggca gcggccaggt ggagtagggt cattccgcgg aaagtctttg agtggatcaa
     8641 gtgcttggac ttcgcccagc aggctcggct catcatcttc tcgcatacca cgaccacacg
     8701 gctctcaaag cagctcccca aggccccagt cccagaagca cactgtgcct ggctccctcc
     8761 attcccgctc ccgctgccgc ctccactgct gccgcctccg ctcgcctgtt tgtgctgctg
     8821 ggaccccgtc atctcggcca tcctcctctc catctgctcc agtcgttcca ggatggacat
     8881 cctgaactgg ttatcgtcca acgacagcca gtcgtgctgg gaggaaggga gcgtgggcag
     8941 agcccgggct ttgtactcaa acaccaccga gttggagatg atctggttgt tgaaggcaac
     9001 ttgtagggtc acaagaccag tgtcatgggc tgggcagtag cagcgcagca ccccaggctg
     9061 aatcagggat gcaggcactg agatctggtc aaacaggcag ctgtagttat tgctggcttc
     9121 ttgccacggg cctgtgatga ggaccttcac tcctccctct gggtaagacc actctgggga
     9181 gtagtcggtc accatgaaca cccgtccgct ctgctgcagc atgcctaggg tgccctgggc
     9241 cgaggcggcc gagaccacct cggcgacgtg catgtaggcc atggtgctgg ccccgccctc
     9301 cgagctgctc agctgcaggc taccctgctg ggggctacag caggggaggc acatctctgc
     9361 ctgggtgaag ggggcccgcg ccccgtcctc tgactgtgag agcgccgtgc tgtcccccga
     9421 caccagctgg tgcccataga tggtgctgct gcccccctcc acggagatga agtcgttgat
     9481 caggtcggag aactggttgc tgaaggagat gtcaaagtgg tccaggctga gctccatgtt
     9541 ggaggcgttg ccgaggctgg gctgggccac ggggtagagt ccctgcacca cgttgcccgg
     9601 gaggatgggg acgccgccgg cgcttcggat taccccgttg gtctccggct gcaggtagtg
     9661 ctcagagctg caagcctgca gctccccaga cttgagcagg acctcgctcc cttccgccgc
     9721 ctgcgaggtc tccatggtga cctccccttc tgccgtcatg gcctggaagt tggcctggaa
     9781 ctgcatgagg tggagggagg aagtggactc gatccgcgtc tccacgtgac cgctgcagga
     9841 gctggtttgg gacgaggcct ccgttttcac cgtgggcatc acgaaggtgc ccccagagtc
     9901 actcaggctg ctgtgggtgt tctgctcgac ggggaggggt ttgctggcgt cctgcaggaa
     9961 gaagctcggg gagggggtct ggtggatgtg ggggctgtgg cccgtctggc tgaagctgga
    10021 cgtgtagtcc ttgttggagt cgatggcgct gaagtccatc tgctccaggg tggtgctggg
    10081 actcaggccg ccgcccgacg gcaggaggct ggagccggcg gtcagggtga gggagctggc
    10141 tgccacagcc gccgcggagg aggagtacgc ttctttggcc atctgctgct cgaaggaggt
    10201 gtcctcggtc ttgatctcct tggtgaggac ggtggtaaag ctgaagctcc gctccccggg
    10261 tggcgagtgc cggatgttgg agctgaccat ctcggctttc aggcctccac ccccgtacgt
    10321 ctggccctgc tttgggttat taaggaaaca gtcggggtca aagttcatgg tggtttctgg
    10381 aatcttccgg ccaccgtcga gggtggtgga gaggaccagc tcttcggaca cgttggtggg
    10441 cagcatggac aggctctccg agctgcccgt ggtgggaaag gcgaacttgt ggccatcaga
    10501 gctcacggcc aggacgaggc cctgagaggc gtcaggtgag aggaggtggt ggttggggcc
    10561 agcggtgggg gacatggtgt acacggcctc attggtgacc tctgacatga acaccgtggc
    10621 gctctgggac aagctcccca tcacagaggc caccgccata ccggacacac ctgtgaccac
    10681 cgggctgtcc accatgtccg ggtcgctgtt gagcccgctg ctgatggaca caggggagct
    10741 ctgggtggtg tcggggactt ccacctggtt ggtggaggag acctcggtgc tgctctggtg
    10801 caggagcacg ggcttggcca ccttgccgtt cctcttctca cggctggagc ccttgctgtg
    10861 gctgtgctcg tgcttgccct ccgacacgtc attgtgctgc acctccgagt ggctcccgta
    10921 cccccctgtc cgtggctcca ccttgggcga gatgatgcgg tgtttggcgc tgttacactt
    10981 gtgatgcacg ctgccgccag ctcccaggct gccggtgcag aggcagttgt gggtccgcgg
    11041 ctggggcttg gtctggtggc tgtcgaggat ctgctgcacc agctgttcca ccgagaagcc
    11101 tgagctgctg ttcccattgc tgcaggtcca cttgatgcca tggaacatgg gtttcagctg
    11161 cccgatgagc tcttctttcg tccatttcgc ccactccttc ttgtcggtgt tgatggagca
    11221 gaggatgggg ccgcaaggct tgccgcagtc ctcgatggcc ggcacgttca ggtagtgcac
    11281 caggacgatg tcggggttct gaaggagcca gtagcacctc cggtggaagg tggggatgat
    11341 ggaggaatgg acatagcagc cgtacaagca ctccactccc tggaccttga gtttcatgtg
    11401 gtcctctctg gtcgttttcc catctttcct ctttttccag caatacccat ctttcctgta
    11461 tttcactttc ttcctgttgt agagtatcat tgagccattc tgtggtcttg tcttagggga
    11521 ggtggttagc cattcttcgt gtttctcaaa tgttattaaa taagctgcaa tttcctcatt
    11581 agtgttccag cggtgcctct cttttggtaa acttgaacat ttcggcagac attcaagcag
    11641 ctttttcggt aaaaagattt ttacatgact actattgctg ttcccatgat catcttctat
    11701 aggtggcacg gtgttgagaa cacagtaggt gctagttccg cagaatacac tttgggaaac
    11761 gctcttccgg cttgttttcg gcagccattt cccctccgcg cgccacatgg tggcggtgct
    11821 agcctcacga cacctgaaat ggaagaaaaa aactttgaac cactgtctga ggcttgagaa
    11881 tgaaccaaga tccaaactca aaaagggcaa attccaagga gaattacatc aagtgccaag
    11941 ctggcctaac ttcagtctcc acccactcag tgtggggaaa ctccatcgca taaaacccct
    12001 ccccccaacc taaagacgac gtactccaaa agctcgagaa ctaatcgagg tgcctggacg
    12061 gcgcccggta ctccgtggag tcacatgaag cgacggctga ggacggaaag gcccttttcc
    12121 tttgtgtggg tgactcaccc gcccgctctc ccgagcgccg cgtcctccat tttgagctcc
    12181 ctgcagcagg gccgggaagc ggccatcttt ccgctcacgc aactggtgcc gaccgggcca
    12241 gccttgccgc ccagggcggg gcgatacacg gcggcgcgag gccaggcacc agagcaggcc
    12301 ggccagcttg agactacccc cgtccgattc tcggtggccg cgctcgcagg ccccgcctcg
    12361 ccgaacatgt gcgctgggac gcacgggccc cgtcgccgcc cgcggcccca aaaaccgaaa
    12421 taccagtgtg cagatcttgg cccgcattta caagactatc ttgccagaaa aaaagcgtcg
    12481 cagcaggtca tcaaaaattt taaatggcta gagacttatc gaaagcagcg agacaggcgc
    12541 gaaggtgcca ccagattcgc acgcggcggc cccagcgccc aggccaggcc tcaactcaag
    12601 cacgaggcga aggggctcct taagcgcaag gcctcgaact ctcccaccca cttccaaccc
    12661 gaagctcggg atcaagaatc acgtactgca gccaggtgga agtaattcaa ggcacgcaag
    12721 ggccataacc cgtaaagagg ccaggcccgc gggaaccaca cacggcactt acctgtgttc
    12781 tggcggcaaa cccgttgcga aaaagaacgt tcacggcgac tactgcactt atatacggtt
    12841 ctcccccacc ctcgggaaaa aggcggagcc agtacacgac atcactttcc cagtttaccc
    12901 cgcgccacct tctctaggca ccggttcaat tgccgacccc tccccccaac ttctcgggga
    12961 ctgtgggcga tgtgcgctct gcccactgac gggcaccgga gccaattccc actcctttca
    13021 agacctagaa ggtccattag ctgcaaagat tcctctctgt ttaaaacttt atccatcttt
    13081 gcacctgcag gcctcgagcc ggcgccaaag tggatctctg ctgtccctgt aataaacccg
    13141 aaaattttga atttttgtaa tttgtttttg taattcttta gtttgtatgt ctgttgctat
    13201 tatgtctact attctttccc ctgcactgta ccccccaatc cccccttttc ttttaaaatt
    13261 gtggatgaat actgccattt gtctcaagat ctagaattcg ttaactggca ccgggcttgc
    13321 gggtcatgca ccaggtgcgc ggtccttcgg gcacctcgac gtcggcggtg acggtgaagc
    13381 cgagccg
//
