LOCUS       sequence-416290-       10469 bp    DNA     circular SYN 15-APR-2025
DEFINITION  doxycycline-inducible expression of Human PRDM1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10469)
  AUTHORS   Altman A, Henriques-Oliveira L, Kurochkin I, Ascic E, Halitzki E, 
            Rosa F, Pires C, Pereira C-F
  TITLE     Transcription Factor Blueprints Underlying Dendritic Cell Diversity
  JOURNAL   Exp Hematol. 2024 Aug. 137:104298
REFERENCE   2  (bases 1 to 10469)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Exp 
            Hematol. 2024 Aug. 137:104298"
COMMENT     Sequence Label: pFUW-TetO-PRDM1
FEATURES             Location/Qualifiers
     source          1..10469
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        27..406
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        408..606
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             624..804
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    851..976
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1469..1702
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1887..1931
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2229..2346
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2403..2421,2422..2444,2445..2463,2464..2486,2487..2505,
                     2506..2528,2529..2547,2548..2570,2571..2589,2590..2612,
                     2613..2631,2632..2654,2655..2673)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2706..2744
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2741..2765
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    4950..5538
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5003..5023)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5421..5432)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5541..5557)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5542..5558)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6063..6243
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6269..6286)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6275..6499
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6545..6973
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6632..6651)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6842..6863
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6982..7002)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6987..7316
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7167..7302
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7229..7248
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7364..7411
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7430..7804
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7934..8055
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7971..7990)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8025..8044
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8104..8120)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8104..8120)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8117..8139)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8128..8144
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8152..8158,8159..8176,8177..8182))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8197..8218
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8335..8352)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8506..9094)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8586..8605)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9265..10056,10057..10125))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9888..9907
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10126..10230)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10305..10324)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
       61 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      121 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      181 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      241 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      301 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      361 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
      421 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
      481 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
      541 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      601 aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
      661 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
      721 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
      781 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
      841 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
      901 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
      961 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     1021 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     1081 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     1141 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     1201 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     1261 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     1321 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     1381 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     1441 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     1501 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     1561 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     1621 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     1681 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     1741 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     1801 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     1861 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     1921 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     1981 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     2041 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     2101 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
     2161 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
     2221 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     2281 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     2341 aattttcggg tttattacag ggacagcaga gatccagttt ggttagatct cgagtttacc
     2401 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     2461 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     2521 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
     2581 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
     2641 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagct cggtacccgg
     2701 gtcgaggtag gcgtgtacgg tgggaggcct atataagcag agctcgttta gtgaaccgtc
     2761 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
     2821 ccagcctccg cggccccgaa ttcaccggta tggaaaagat ctattccaga ggggagcttc
     2881 accacttcat tgacggcttt aatgaagaga aaagcaactg gatgcgctat gtgaatccag
     2941 cacactctcc ccgggagcaa aacctggctg cgtgtcagaa cgggatgaac atctacttct
     3001 acaccattaa gcccatccct gccaaccagg aacttcttgt gtggtattgt cgggactttg
     3061 cagaaaggct tcactaccct tatcccggag agctgacaat gatgaatctc acacaaacac
     3121 agagcagtct aaagcaaccg agcactgaga aaaatgaact ctgcccaaag aatgtcccaa
     3181 agagagagta cagcgtgaaa gaaatcctaa aattggactc caacccctcc aaaggaaagg
     3241 acctctaccg ttctaacatt tcacccctca catcagaaaa ggacctcgat gactttagaa
     3301 gacgtgggag ccccgaaatg cccttctacc ctcgggtcgt ttaccccatc cgggcccctc
     3361 tgccagaaga ctttttgaaa gcttccctgg cctacgggat cgagagaccc acgtacatca
     3421 ctcgctcccc cattccatcc tccaccactc caagcccctc tgcaagaagc agccccgacc
     3481 aaagcctcaa gagctccagc cctcacagca gccctgggaa tacggtgtcc cctgtgggcc
     3541 ccggctctca agagcaccgg gactcctacg cttacttgaa cgcgtcctac ggcacggaag
     3601 gtttgggctc ctaccctggc tacgcacccc tgccccacct cccgccagct ttcatcccct
     3661 cgtacaacgc tcactacccc aagttcctct tgccccccta cggcatgaat tgtaatggcc
     3721 tgagcgctgt gagcagcatg aatggcatca acaactttgg cctcttcccg aggctgtgcc
     3781 ctgtctacag caatctcctc ggtgggggca gcctgcccca ccccatgctc aaccccactt
     3841 ctctcccgag ctcgctgccc tcagatggag cccggaggtt gctccagccg gagcatccca
     3901 gggaggtgct tgtcccggcg ccccacagtg ccttctcctt taccggggcc gccgccagca
     3961 tgaaggacaa ggcctgtagc cccacaagcg ggtctcccac ggcgggaaca gccgccacgg
     4021 cagaacatgt ggtgcagccc aaagctacct cagcagcgat ggcagccccc agcagcgacg
     4081 aagccatgaa tctcattaaa aacaaaagaa acatgaccgg ctacaagacc cttccctacc
     4141 cgctgaagaa gcagaacggc aagatcaagt acgaatgcaa cgtttgcgcc aagactttcg
     4201 gccagctctc caatctgaag gtccacctga gagtgcacag tggagaacgg cctttcaaat
     4261 gtcagacttg caacaagggc tttactcagc tcgcccacct gcagaaacac tacctggtac
     4321 acacgggaga aaagccacat gaatgccagg tctgccacaa gagatttagc agcaccagca
     4381 atctcaagac ccacctgcga ctccattctg gagagaaacc ataccaatgc aaggtgtgcc
     4441 ctgccaagtt cacccagttt gtgcacctga aactgcacaa gcgtctgcac acccgggagc
     4501 ggccccacaa gtgctcccag tgccacaaga actacatcca tctctgtagc ctcaaggttc
     4561 acctgaaagg gaactgcgct gcggccccgg cgcctgggct gcccttggaa gatctgaccc
     4621 gaatcaatga agaaatcgag aagtttgaca tcagtgacaa tgctgaccgg ctcgaggacg
     4681 tggaggatga catcagtgtg atctctgtag tggagaagga aattctggcc gtggtcagaa
     4741 aagagaaaga agaaactggc ctgaaagtgt ctttgcaaag aaacatgggg aatggactcc
     4801 tctcctcagg gtgcagcctt tatgagtcat cagatctacc cctcatgaag ttgcctccca
     4861 gcaacccact acctctggta cctgtaaagg tcaaacaaga aacagttgaa ccaatggatc
     4921 cttaattcga agatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa
     4981 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     5041 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     5101 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     5161 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     5221 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     5281 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     5341 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     5401 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     5461 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     5521 tttgggccgc ctccccgcat cgataccgtc gacctcgaga cctagaaaaa catggagcaa
     5581 tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg
     5641 aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg
     5701 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     5761 cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg
     5821 attggcagaa ctacacacca gggccaggga tcagatatcc actgaccttt ggatggtgct
     5881 acaagctagt accagttgag caagagaagg tagaagaagc caatgaagga gagaacaccc
     5941 gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtattagagt
     6001 ggaggtttga cagccgccta gcatttcatc acatggcccg agagctgcat ccggactgta
     6061 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     6121 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     6181 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     6241 gcagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     6301 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     6361 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     6421 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     6481 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat
     6541 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     6601 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     6661 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     6721 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     6781 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     6841 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     6901 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     6961 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     7021 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     7081 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     7141 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     7201 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
     7261 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     7321 tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgttgaca attaatcatc
     7381 ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca tggccaagtt
     7441 gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac
     7501 cgaccggctc gggttctccc gggacttcgt ggaggacgac ttcgccggtg tggtccggga
     7561 cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca acaccctggc
     7621 ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag tggtcggagg tcgtgtccac
     7681 gaacttccgg gacgcctccg ggccggccat gaccgagatc ggcgagcagc cgtgggggcg
     7741 ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga
     7801 ctgacacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     7861 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     7921 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     7981 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     8041 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
     8101 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     8161 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     8221 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     8281 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     8341 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     8401 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     8461 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     8521 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     8581 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     8641 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     8701 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     8761 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     8821 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     8881 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     8941 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     9001 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     9061 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     9121 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     9181 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     9241 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     9301 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     9361 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     9421 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     9481 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     9541 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     9601 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     9661 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     9721 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     9781 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     9841 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     9901 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     9961 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
    10021 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
    10081 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
    10141 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
    10201 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
    10261 cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat
    10321 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc
    10381 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg
    10441 cttagggtta ggcgttttgc gctgcttcg
//
