LOCUS       sequence-416292-       10181 bp    DNA     circular SYN 15-APR-2025
DEFINITION  doxycycline-inducible expression of Human ARID3A in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10181)
  AUTHORS   Altman A, Henriques-Oliveira L, Kurochkin I, Ascic E, Halitzki E, 
            Rosa F, Pires C, Pereira C-F
  TITLE     Transcription Factor Blueprints Underlying Dendritic Cell Diversity
  JOURNAL   Exp Hematol. 2024 Aug. 137:104298
REFERENCE   2  (bases 1 to 10181)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Exp 
            Hematol. 2024 Aug. 137:104298"
COMMENT     Sequence Label: pFUW-TetO-ARID3A
FEATURES             Location/Qualifiers
     source          1..10181
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        27..406
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        408..606
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             624..804
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    851..976
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1469..1702
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1887..1931
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2229..2346
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2403..2421,2422..2444,2445..2463,2464..2486,2487..2505,
                     2506..2528,2529..2547,2548..2570,2571..2589,2590..2612,
                     2613..2631,2632..2654,2655..2673)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2706..2744
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2741..2765
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      4306..4315
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    4662..5250
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4715..4735)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5133..5144)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5253..5269)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5254..5270)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5775..5955
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5981..5998)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5987..6211
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6257..6685
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6344..6363)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6554..6575
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6694..6714)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6699..7028
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6879..7014
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6941..6960
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7076..7123
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7142..7516
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7646..7767
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7683..7702)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7737..7756
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7816..7832)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7816..7832)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7829..7851)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7840..7856
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7864..7870,7871..7888,7889..7894))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7909..7930
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8047..8064)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8218..8806)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8298..8317)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8977..9768,9769..9837))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9600..9619
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9838..9942)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10017..10036)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
       61 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      121 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      181 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      241 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      301 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      361 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
      421 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
      481 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
      541 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      601 aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
      661 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
      721 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
      781 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
      841 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
      901 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
      961 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     1021 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     1081 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     1141 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     1201 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     1261 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     1321 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     1381 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     1441 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     1501 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     1561 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     1621 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     1681 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     1741 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     1801 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     1861 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     1921 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     1981 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     2041 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     2101 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
     2161 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
     2221 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     2281 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     2341 aattttcggg tttattacag ggacagcaga gatccagttt ggttagatct cgagtttacc
     2401 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     2461 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     2521 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
     2581 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
     2641 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagct cggtacccgg
     2701 gtcgaggtag gcgtgtacgg tgggaggcct atataagcag agctcgttta gtgaaccgtc
     2761 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
     2821 ccagcctccg cggccccgaa ttcaccggtt ctagaatgaa actacaggcc gtgatggaga
     2881 cgctgttgca gcggcagcag cgggcgcgcc aggagctgga ggcccggcag cagctgcccc
     2941 ccgatccccc tgctgcaccc cacggccggg cccgggctgc ccccgacgag gacagagagc
     3001 ccgaaagtgc ccggatgcag cgggctcaga tggccgcact ggcagccatg cgggctgcag
     3061 ctgcgggcct gggacacccg gccagccccg gcggctctga ggatgggccc ccaggctcgg
     3121 aggaggagga cgcggcccgg gaggggacac cgggctcacc cgggcgaggc agagaagggc
     3181 caggagagga gcactttgag gacatggcct ccgacgagga catgaagccc aaatgggagg
     3241 aggaggagat ggaggaagac ctcggggagg atgaggagga ggaggaggag gattacgagg
     3301 atgaggagga ggaggaggac gaggaggggc tgggcccccc aggccctgcc agcttgggca
     3361 ccacggcact gttcccccga aaggcccagc caccccaggc cttccgcggc gatggcgttc
     3421 ccagggtgct ggggggccag gagcggccgg ggcctggccc tgcccacccc ggaggggccg
     3481 cccacgtagc cccgcagctg cagccgcctg accacggcga ctggacttac gaggagcagt
     3541 ttaagcagct ctacgaactc gacggggacc ccaagaggaa ggaattcctg gatgacttgt
     3601 tcagcttcat gcagaagcga gggacacctg tgaaccgcat ccccatcatg gccaaacagg
     3661 tccttgacct gttcatgctg tacgtgctgg tgacggagaa gggcggcctc gtggaggtca
     3721 tcaacaagaa gctgtggcgt gagatcacca agggcctcaa cctgcccacg tccatcacca
     3781 gtgcagcctt caccctgcgg acccaataca tggagtacct gtacccctac gagtgtgaga
     3841 agcggggcct cagtaacccc aatgagctcc aggcagccat agacagcaac cgacgggagg
     3901 gccggcgcca gagctttggt ggctccctct ttgcctactc gccaggcggg gcacacggca
     3961 tgctctcctc acccaagcta cccgtgtcct ccctgggcct ggccgcaagc accaacggca
     4021 gctccatcac ccccgcccct aagatcaaga aagaggagga ctcagccatc cccatcacag
     4081 tccctggccg cctgcctgtg tccctggcgg gccaccctgt ggtggcagcc caggcagcag
     4141 ctgtgcaagc agcagccgcc caagcagctg tggctgcaca ggcagctgcc ctggaacagc
     4201 tgcgggagaa gctggagtct gcggagcctc cggagaagaa gatggccctg gtggccgatg
     4261 agcagcaacg gctgatgcaa cgtgcactcc agcagaactt cctggccatg gcggcccagc
     4321 tgcccatgag cattcggatc aacagccaag cctccgaaag ccgccaggac tctgctgtga
     4381 acctgacggg caccaacggc agcaacagca tcagcatgtc ggtggagatc aacggcatca
     4441 tgtacacagg agttctgttt gctcagccgc cggcccccac gccaacctct gctcccaaca
     4501 aaggaggcgg cggcggcggc agcagcagca gcaacgcagg cggccgggga ggaaacaccg
     4561 gaaccagcgg cggccaggct gggccagcgg ggctgtccac accctccaca tctacctcaa
     4621 ataactcgtt gccttaattc gaagatatca agcttatcga taatcaacct ctggattaca
     4681 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     4741 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     4801 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     4861 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     4921 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     4981 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     5041 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
     5101 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     5161 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     5221 gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga gacctagaaa
     5281 aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag
     5341 aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa
     5401 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     5461 ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag
     5521 gctacttccc tgattggcag aactacacac cagggccagg gatcagatat ccactgacct
     5581 ttggatggtg ctacaagcta gtaccagttg agcaagagaa ggtagaagaa gccaatgaag
     5641 gagagaacac ccgcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag
     5701 aagtattaga gtggaggttt gacagccgcc tagcatttca tcacatggcc cgagagctgc
     5761 atccggactg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     5821 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     5881 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     5941 ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt
     6001 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     6061 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     6121 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     6181 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
     6241 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     6301 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     6361 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     6421 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     6481 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     6541 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     6601 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     6661 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa
     6721 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     6781 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     6841 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     6901 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     6961 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     7021 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgttga
     7081 caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac
     7141 catggccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt
     7201 cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg
     7261 tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga
     7321 caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga
     7381 ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca
     7441 gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc
     7501 cgaggagcag gactgacacg tgctacgaga tttcgattcc accgccgcct tctatgaaag
     7561 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     7621 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
     7681 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     7741 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
     7801 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     7861 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     7921 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     7981 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     8041 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     8101 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     8161 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     8221 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     8281 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     8341 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     8401 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     8461 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     8521 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     8581 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     8641 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     8701 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     8761 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     8821 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     8881 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     8941 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     9001 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     9061 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     9121 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     9181 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     9241 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     9301 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     9361 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     9421 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     9481 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     9541 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     9601 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     9661 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     9721 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     9781 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     9841 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     9901 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     9961 gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca
    10021 atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc
    10081 gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc
    10141 atgaagaatc tgcttagggt taggcgtttt gcgctgcttc g
//
