LOCUS       sequence-416301-       10262 bp    DNA     circular SYN 15-APR-2025
DEFINITION  doxycycline-inducible expression of Mouse Cbfa2t3 in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10262)
  AUTHORS   Altman A, Henriques-Oliveira L, Kurochkin I, Ascic E, Halitzki E, 
            Rosa F, Pires C, Pereira C-F
  TITLE     Transcription Factor Blueprints Underlying Dendritic Cell Diversity
  JOURNAL   Exp Hematol. 2024 Aug. 137:104298
REFERENCE   2  (bases 1 to 10262)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Exp 
            Hematol. 2024 Aug. 137:104298"
COMMENT     Sequence Label: pFUW-TetO-Cbfa2t3
FEATURES             Location/Qualifiers
     source          1..10262
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        27..406
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        408..606
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             624..804
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    851..976
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1469..1702
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1887..1931
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2229..2346
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2403..2421,2422..2444,2445..2463,2464..2486,2487..2505,
                     2506..2528,2529..2547,2548..2570,2571..2589,2590..2612,
                     2613..2631,2632..2654,2655..2673)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2706..2744
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2741..2765
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    4743..5331
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4796..4816)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5214..5225)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5334..5350)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5335..5351)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5856..6036
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6062..6079)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6068..6292
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6338..6766
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6425..6444)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6635..6656
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6775..6795)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6780..7109
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6960..7095
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7022..7041
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7157..7204
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7223..7597
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7727..7848
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7764..7783)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7818..7837
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7897..7913)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7897..7913)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7910..7932)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7921..7937
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7945..7951,7952..7969,7970..7975))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7990..8011
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8128..8145)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8299..8887)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8379..8398)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9058..9849,9850..9918))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9681..9700
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9919..10023)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10098..10117)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
       61 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      121 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      181 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      241 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      301 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      361 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
      421 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
      481 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
      541 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      601 aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
      661 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
      721 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
      781 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
      841 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
      901 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
      961 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     1021 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     1081 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     1141 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     1201 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     1261 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     1321 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     1381 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     1441 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     1501 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     1561 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     1621 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     1681 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     1741 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     1801 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     1861 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     1921 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     1981 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     2041 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     2101 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
     2161 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
     2221 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     2281 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     2341 aattttcggg tttattacag ggacagcaga gatccagttt ggttagatct cgagtttacc
     2401 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     2461 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     2521 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
     2581 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
     2641 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagct cggtacccgg
     2701 gtcgaggtag gcgtgtacgg tgggaggcct atataagcag agctcgttta gtgaaccgtc
     2761 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
     2821 ccagcctccg cggccccgaa ttcaccggtt ctagaatgtc ccaggcatcc accaccactc
     2881 tggagagtgg ggcactcctc tcgggacccc ggggtcttca gaatggaagc ccagctcaca
     2941 ggaaggagaa ggctgcagca atgccagact ccccggctga agtgaagacg cagccccggt
     3001 ccacaccccc cagcatgccg cccccacctc ccacctcatc ccagggagcc acacgaccac
     3061 cctccttcac acctcacaca catggcgagg atggacctgc gacgtctctt ccccatggcc
     3121 gtttccacgg ctgcttaaag tggtccatgg tctgtctctt gatgaacggc agcagccact
     3181 cacccacggc catccacggt gccccatcta cacccaatgg cttcagcaac ggcccagcca
     3241 cctcatccac ggcctcgctc tccacacagc acctgccccc ggcgtgcggg gcacggcagc
     3301 tcagcaagct taagcgtttc ctcaccaccc tacagcagtt tggcagtgac atctcacctg
     3361 agatcgggga gcgcgtgcgc acactggtgc tgggcctggt gaactcaact ctgacgatcg
     3421 aagagtttca tgccaagctc caggaagcca ccaactttcc actgaggccg tttgttatcc
     3481 cttttctgaa ggctaatctt ccactgctgc agcgtgagct cctgcactgt gcccgcctgg
     3541 ccaaacagac acctgcccag tacctggccc agcacgaaca gctgctgctg gacgccagcg
     3601 ccacctcccc tgtcgactcg tctgagctcc tgctggaagt caacgagaac ggcaaaagga
     3661 gaacacctga caggaccaaa gagaatggat cagaccggga ccctctgcac cccgaccacc
     3721 tcagtaagcg gtcctgcacc ctgagccccg cccagcgctg cagccccagc aatgggctgc
     3781 cccacccgac gccaccccca cccccgcact atcgcctgga ggacatggcc atggcccacc
     3841 atttccggga ctcctaccgc catcctgatc cccgagagct acgggaacgc caccggcccc
     3901 tggccatacc tgggtctcga caagaagaag tgattgatca caggctcaca gaacgcgagt
     3961 gggcagaaga atggaagcac ctcaacagtc ttctgaactg catcatggac atggtggaga
     4021 agacccggcg atccctcacc gtcctgcgcc ggtgtcagga ggccgaccgt gaggaactca
     4081 accactggat ccggtgctac agtgactctg aggaggggaa gaagggccct acccccatct
     4141 ctgcccggtc cctcaacagc tgcagtggcc ctgaggggtc tcagctagat gttcaccggg
     4201 acttcacgcc caggaccctg tctggctaca tgcctgaaga gatctggagg aaggctgaag
     4261 aagctgtgaa tgaggtgaag cgccaggcca tgtcagaact acagaaagct gtgtctgatg
     4321 cggagcgcaa agcccatgaa ctcatcacca cagagcgtgc caagatggaa cgagccctgg
     4381 cggaggccaa gcgacaggcc tcggaggatg ccctgactgt catcaaccag caagaggact
     4441 ccagcgagag ctgctggaac tgcgggcgca aggccagcga gacgtgcagt ggctgtaacg
     4501 ccgcccgcta ctgcgggtcc ttctgtcagc acaaagactg ggagaaacac catcacgtgt
     4561 gcggccagag tctgcagggc cccgcggctg cagtggctga cccactacct ggacagcctg
     4621 acgccactgc cagccccagc gaagccggct cggcagggcc ctctcgtccc tgctctccgg
     4681 ggcctccagg cccgctggac gctgctgtgc cccgctgatt cgaagatatc aagcttatcg
     4741 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     4801 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     4861 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     4921 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     4981 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     5041 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     5101 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     5161 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     5221 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     5281 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc
     5341 gtcgacctcg agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca
     5401 atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac
     5461 ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     5521 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc
     5581 tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca ccagggccag
     5641 ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagcaagaga
     5701 aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg agcctgcatg
     5761 ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc ctagcatttc
     5821 atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta gaccagatct
     5881 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     5941 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     6001 tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac ccgctgatca
     6061 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     6121 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     6181 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     6241 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     6301 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     6361 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     6421 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     6481 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     6541 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     6601 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     6661 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     6721 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
     6781 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     6841 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     6901 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     6961 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     7021 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     7081 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     7141 gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata
     7201 cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc
     7261 gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt
     7321 cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt
     7381 ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga
     7441 gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc
     7501 catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg
     7561 caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc
     7621 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     7681 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
     7741 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     7801 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
     7861 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     7921 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     7981 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     8041 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     8101 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     8161 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     8221 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     8281 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     8341 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     8401 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     8461 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     8521 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     8581 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     8641 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     8701 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     8761 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     8821 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     8881 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     8941 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     9001 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     9061 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     9121 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     9181 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     9241 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     9301 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     9361 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     9421 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     9481 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     9541 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     9601 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     9661 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     9721 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     9781 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     9841 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     9901 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     9961 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    10021 gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc
    10081 ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc
    10141 cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg
    10201 caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt
    10261 cg
//
