LOCUS       sequence-416327-        9920 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Protein quality control substrate and associated control YIp 
            ADE2-LEU2 pTDH3::aro7-R33P-GFP::tADH1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9920)
  AUTHORS   Flagg MP, Lam B, Lam DK, Le TM, Kao A, Slaiwa YI, Hampton RY
  TITLE     Exploring the ""misfolding problem"" by systematic discovery and 
            analysis of functional-but-degraded proteins.
  JOURNAL   Mol Biol Cell. 2023 Dec 1;34(13):ar125. doi: 
            10.1091/mbc.E23-06-0248. Epub 2023 Sep 20.
  PUBMED    37729018
REFERENCE   2  (bases 1 to 9920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2023-12-1"; volume: "34(13):ar125. doi"; pages: " 
            10.1091/mbc.E23-06-0248. Epub 2023 Sep 20"
COMMENT     Sequence Label: pRH3243
FEATURES             Location/Qualifiers
     source          1..9920
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        22..665
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     558..581
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     primer_bind     complement(697..713)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(698..714)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             1515..2225
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(1542..1570)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2165..2185
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      2249..2436
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2304..2328)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(2440..2459)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2441..2459)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2469..2486)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2469..2485)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2478..2500)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2626..3081
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2713..2732)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2923..2944
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(3286..5001)
                     /codon_start=1
                     /gene="S. cerevisiae ADE2"
                     /product="phosphoribosylaminoimidazole carboxylase, 
                     required for adenine biosynthesis"
                     /label=ADE2
                     /note="yeast auxotrophic marker"
                     /translation="MDSRTVGILGGGQLGRMIVEAANRLNIKTVILDAENSPAKQISNS
                     NDHVNGSFSNPLDIEKLAEKCDVLTIEIEHVDVPTLKNLQVKHPKLKIYPSPETIRLIQ
                     DKYIQKEHLIKNGIAVTQSVPVEQASETSLLNVGRDLGFPFVLKSRTLAYDGRGNFVVK
                     NKEMIPEALEVLKDRPLYAEKWAPFTKELAVMIVRSVNGLVFSYPIVETIHKDNICDLC
                     YAPARVPDSVQLKAKLLAENAIKSFPGCGIFGVEMFYLETGELLINEIAPRPHNSGHYT
                     IDACVTSQFEAHLRSILDLPMPKNFTSFSTITTNAIMLNVLGDKHTKDKELETCERALA
                     TPGSSVYLYGKESRPNRKVGHINIIASSMAECEQRLNYITGRTDIPIKISVAQKLDLEA
                     MVKPLVGIIMGSDSDLPVMSAACAVLKDFGVPFEVTIVSAHRTPHRMSAYAISASKRGI
                     KTIIAGAGGAAHLPGMVAAMTPLPVIGVPVKGSCLDGVDSLHSIVQMPRGVPVATVAIN
                     NSTNAALLAVRLLGAYDSSYTTKMEQFLLKQEEEVLVKAQKLETVGYEAYLENK"
     promoter        complement(5002..5377)
                     /gene="S. cerevisiae ADE2"
                     /label=ADE2 promoter
     primer_bind     complement(5568..5587)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5687..5709
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5747..5765)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             complement(6051..7145)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(7146..7553)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     promoter        7721..7825
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7826..7894,7895..8686)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8044..8063)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8857..9445
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9346..9365
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9599..9616
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9733..9754
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9769..9774,9775..9792,9793..9799)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9807..9823
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9812..9834
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9831..9847
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9831..9847
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9866..9886
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9868..9886
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 gctgcttggc ggtcgagttt atcattatca atactgccat ttcaaagaat acgtaaataa
       61 ttaatagtag tgattttcct aactttattt agtcaaaaaa ttagcctttt aattctgctg
      121 taacccgtac atgcccaaaa tagggggcgg gttacacaga atatataaca tcgtaggtgt
      181 ctgggtgaac agtttattcc tggcatccac taaatataat ggagcccgct ttttaagctg
      241 gcatccagaa aaaaaaagaa tcccagcacc aaaatattgt tttcttcacc aaccatcagt
      301 tcataggtcc attctcttag cgcaactaca gagaacaggg gcacaaacag gcaaaaaacg
      361 ggcacaacct caatggagtg atgcaacctg cctggagtaa atgatgacac aaggcaattg
      421 acccacgcat gtatctatct cattttctta caccttctat taccttctgc tctctctgat
      481 ttggaaaaag ctgaaaaaaa aggttgaaac cagttccctg aaattattcc cctacttgac
      541 taataagtat ataaagacgg taggtattga ttgtaattct gtaaatctat ttcttaaact
      601 tcttaaattc tacttttata gttagtcttt tttttagttt taaaacacca agaacttagt
      661 ttcgacggat ctgcaggaat tcgatatcaa gcttatcgat accgtcgacc tcgagagata
      721 tggatttcac aaaaccagaa actgttttaa atctacaaaa tattagagat gaattagtta
      781 gaatggagga ttcgatcatc ttcaaattta ttgagccatc gcatttcgcc acatgtcctt
      841 cagtttatga ggcaaaccat ccaggtttag aaattccgaa ttttaaagga tctttcttgg
      901 attgggctct ttcaaatctt gaaattgcgc attctcgcat cagaagattc gaatcacctg
      961 atgaaactcc cttctttcct gacaagattc agaaatcatt cttaccgagc attaactacc
     1021 cacaaatttt ggcgccttat gccccagaag ttaattacaa tgataaaata aaaaaagttt
     1081 atattgaaaa gattatacca ttaatttcga aaagagatgg tgatgataag aataacttcg
     1141 gttctgttgc cactagagat atagaatgtt tgcaaagctt gagtaggaga atccactttg
     1201 gcaagtttgt tgctgaagcc aagttccaat cggatatccc gctatacaca aagctgatca
     1261 aaagtaaaga tgtcgagggg ataatgaaga atatcaccaa ttctgccgtt gaagaaaaga
     1321 ttctagaaag attaactaag aaggctgaag tctatggtgt ggaccctacc aacgagtcag
     1381 gtgaaagaag gattactcca gaatatttgg taaaaattta taaggaaatt gttataccta
     1441 tcactaagga agttgaggtg gaatacttgc taagaaggtt ggaagagggt cgacggatcc
     1501 ccgggttaat taacagtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg
     1561 aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg
     1621 caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat
     1681 ggccaacact tgtcactact ttcacttatg gtgttcaatg cttttcaaga tacccagatc
     1741 atatgaaacg gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa
     1801 ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg
     1861 atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc
     1921 ttggacacaa attggaatac aactataact cacacaatgt atacatcatg gcagacaaac
     1981 aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggaagcgttc
     2041 aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag
     2101 acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc
     2161 acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat
     2221 acaaataggg cgcgccactt ctaaataagc gaatttctta tgatttatga tttttattat
     2281 taaataagtt ataaaaaaaa taagtgtata caaattttaa agtgactctt aggttttaaa
     2341 acgaaaattc ttattcttga gtaactcttt cctgtaggtc aggttgcttt ctcaggtata
     2401 gtatgaggtc gctcttattg accacacctc taccggcagc cctatagtga gtcgtattac
     2461 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     2521 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     2581 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggacgcg ccctgtagcg
     2641 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     2701 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     2761 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     2821 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     2881 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     2941 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     3001 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     3061 aaatattaac gcttacaatt tcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     3121 ttcacaccgc atagatctta tgtatgaaat tcttaaaaaa ggacacctgt aagcgttgat
     3181 ttctatgtat gaagtccaca tttgatgtaa tcataacaaa gcctaaaaaa taggtatatc
     3241 attttataat tatttgctgt acaagtatat caataaactt atatattact tgttttctag
     3301 ataagcttcg taaccgacag tttctaactt ttgtgctttg acaagaactt cttcttcttg
     3361 ctttaataaa aactgttcca ttttcgttgt ataacttgaa tcataagcgc caagcagtct
     3421 gacagccaac agcgcagcgt tcgtactatt attaatagcg acggtagcta ctggaacacc
     3481 tctaggcatt tgcacaattg aatgtaaaga atctactcca tctagacaag aaccttttac
     3541 gggcacaccg atgacaggaa gtggtgtcat tgcagccacc atacctggca agtgagcagc
     3601 cccaccagct ccagcgataa ttgttttaat tccacgcttg cttgcggaaa tagcatatgc
     3661 tgacatccta tgtggagttc tatgagcaga gactattgtc acttcaaatg gaacgccaaa
     3721 atcttttaaa accgcacatg cggcagacat taccggcaag tcagagtctg atcccatgat
     3781 gattccaacc aatggtttga ccattgcttc caagtccaac ttttgagcga cagagatttt
     3841 gattggaata tcagttctac ctgtaatgta gttcagcctt tgttcacatt ccgccatact
     3901 ggaggcaata atatttatgt gacctacttt tctgttaggt ctagactctt ttccatataa
     3961 gtacactgag gaacctggag tcgccaatgc tctttcgcaa gtttctagct ctttatcttt
     4021 tgtatgtttg tctccaagaa catttagcat aatggcgttc gttgtaatgg tggagaaaga
     4081 tgtgaaattc tttggcattg gcaaatccaa tattgatctc aaatgagctt caaattgaga
     4141 agtgacgcaa gcatcaatgg tataatgtcc agagttgtga ggccttgggg caatttcgtt
     4201 aataagcaat tcccctgttt ctaaatagaa catttccaca ccaaatatac cacaaccggg
     4261 aaaagatttg attgcatttt ctgccaacaa cttcgcctta agttgaacgg agtccggaac
     4321 tctagcaggc gcataacata agtcacaaat attgtccttg tggatagtct ctacaattgg
     4381 gtaagaaaac actaaaccgt taacagacct cacaatcatg actgctaatt ctttagtaaa
     4441 tggtgcccat ttttcggcgt acaaaggacg atccttcagt acttccaaag cttccggaat
     4501 catttcctta ttctttacaa cgaagttacc tcttccatcg tatgccaaag tcctcgactt
     4561 caagacgaat ggaaaaccca aatctcttcc aacattcaat agggacgtct cactggcttg
     4621 ttccacagga acactttggg taactgctat accatttttg attaaatgct ctttttgaat
     4681 atatttgtct tgtatcaatc tgattgtttc tggagaaggg taaattttta atttgggatg
     4741 ttttacttga agattcttta gtgtaggaac atcaacatgc tcaatctcaa tcgttagcac
     4801 atcacatttt tcagctagtt tttcgatatc aagaggattg gaaaaggagc cattaacgtg
     4861 gtcattggag ttgcttattt gtttggcagg agaattttca gcatctagta ttaccgtctt
     4921 aatgttgagc ctgtttgctg cctcaacaat catacgtccc aattgtcccc ctcctaatat
     4981 accaactgtt ctagaatcca tacttgattg ttttgtccga ttttcttgtt tttcttgatt
     5041 gttatagtag gatgtactta gaagagagat ccaacgattt tacgcaccaa tttatacatg
     5101 aaatgctcca taatattgtc catttagttc ttaataaaag gtcagcaaga gtcaatcact
     5161 tagtattacc cggttcgtag ccatgcaaca agagtcattt gtcagcatag ctgtaataat
     5221 caatcatgac gtaagaaatg tatcataatt aaaagttgtt aaagatgtca gtgttatgtt
     5281 ggtgttacaa aattctcggc ttctcactaa tatttaatat ctcttaaatt ttatctgtct
     5341 ttgattcttt taagaaaagt tatgtattat tcaagaatta attcagatct attcttttga
     5401 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     5461 atttaacgcg aattttaaca aaatattaac gcttacaatt tcctgatgcg gtattttctc
     5521 cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct
     5581 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     5641 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     5701 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
     5761 ctatttttat aggttaatgt catgatcttt ttgtgtggtg ccctcctcct tgtcaatatt
     5821 aatgttaaag tgcaattctt tttccttatc acgttgagcc attagtatca atttgcttac
     5881 ctgtattcct ttacatcctc ctttttctcc ttcttgataa atgtatgtag attgcgtata
     5941 tagtttcgtc taccctatga acatattcca ttttgtaatt tcgtgtcgtt tctattatga
     6001 atttcattta taaagtttat gtacaaatat cataaaaaaa gagaatcttt ttaagcaagg
     6061 attttcttaa cttcttcggc gacagcatca ccgacttcgg tggtactgtt ggaaccacct
     6121 aaatcaccag ttctgatacc tgcatccaaa acctttttaa ctgcatcttc aatggcctta
     6181 ccttcttcag gcaagttcaa tgacaatttc aacatcattg cagcagacaa gatagtggcg
     6241 atagggttga ccttattctt tggcaaatct ggagcagaac cgtggcatgg ttcgtacaaa
     6301 ccaaatgcgg tgttcttgtc tggcaaagag gccaaggacg cagatggcaa caaacccaag
     6361 gaacctggga taacggaggc ttcatcggag atgatatcac caaacatgtt gctggtgatt
     6421 ataataccat ttaggtgggt tgggttctta actaggatca tggcggcaga atcaatcaat
     6481 tgatgttgaa ccttcaatgt agggaattcg ttcttgatgg tttcctccac agtttttctc
     6541 cataatcttg aagaggccaa aacattagct ttatccaagg accaaatagg caatggtggc
     6601 tcatgttgta gggccatgaa agcggccatt cttgtgattc tttgcacttc tggaacggtg
     6661 tattgttcac tatcccaagc gacaccatca ccatcgtctt cctttctctt accaaagtaa
     6721 atacctccca ctaattctct gacaacaacg aagtcagtac ctttagcaaa ttgtggcttg
     6781 attggagata agtctaaaag agagtcggat gcaaagttac atggtcttaa gttggcgtac
     6841 aattgaagtt ctttacggat ttttagtaaa ccttgttcag gtctaacact accggtaccc
     6901 catttaggac cacccacagc acctaacaaa acggcatcag ccttcttgga ggcttccagc
     6961 gcctcatctg gaagtggaac acctgtagca tcgatagcag caccaccaat taaatgattt
     7021 tcgaaatcga acttgacatt ggaacgaaca tcagaaatag ctttaagaac cttaatggct
     7081 tcggctgtga tttcttgacc aacgtggtca cctggcaaaa cgacgatctt cttaggggca
     7141 gacattagaa tggtatatcc ttgaaatata tatatatata ttgctgaaat gtaaaaggta
     7201 agaaaagtta gaaagtaaga cgattgctaa ccacctattg gaaaaaacaa taggtcctta
     7261 aataatattg tcaacttcaa gtattgtgat gcaagcattt agtcatgaac gcttctctat
     7321 tctatatgaa aagccggttc cggcgctctc acctttcctt tttctcccaa tttttcagtt
     7381 gaaaaaggta tatgcgtcag gcgacctctg aaattaacaa aaaatttcca gtcatcgaat
     7441 ttgattctgt gcgatagcgc ccctgtgtgt tctcgttatg ttgaggaaaa aaataatggt
     7501 tgctaagaga ttcgaactct tgcatcttac gatacctgag tattcccaca gttaactgcg
     7561 gtcaagatat ttcttgaatc aggcgcctta gaccgctcgg ccaaacaacc aattacttgt
     7621 tgagaaatag agtataatta tcctataaat ataacgtttt tgaacacaca tgaacaagga
     7681 agtacaggag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     7741 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     7801 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     7861 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     7921 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     7981 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     8041 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     8101 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     8161 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     8221 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     8281 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     8341 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     8401 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     8461 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     8521 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     8581 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     8641 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     8701 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     8761 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     8821 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     8881 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     8941 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     9001 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     9061 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     9121 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     9181 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     9241 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     9301 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     9361 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     9421 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     9481 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     9541 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     9601 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     9661 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     9721 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     9781 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     9841 ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga
     9901 gctccaccgc ggtggcggcc
//
