LOCUS       sequence-416334-        9862 bp    DNA     circular SYN 15-APR-2025
DEFINITION  doxycycline-inducible expression of Human RBPJ in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9862)
  AUTHORS   Altman A, Henriques-Oliveira L, Kurochkin I, Ascic E, Halitzki E, 
            Rosa F, Pires C, Pereira C-F
  TITLE     Transcription Factor Blueprints Underlying Dendritic Cell Diversity
  JOURNAL   Exp Hematol. 2024 Aug. 137:104298
REFERENCE   2  (bases 1 to 9862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Exp 
            Hematol. 2024 Aug. 137:104298"
COMMENT     Sequence Label: pFUW-TetO-RBPJ
FEATURES             Location/Qualifiers
     source          1..9862
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        303..341
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     338..362
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    1940..2528
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1993..2013)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2411..2422)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2531..2547)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2532..2548)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3053..3233
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3259..3276)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3265..3489
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3535..3963
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3622..3641)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3832..3853
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3972..3992)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3977..4306
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4157..4292
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4219..4238
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4354..4401
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4420..4794
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    4924..5045
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4961..4980)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5015..5034
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5094..5110)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5094..5110)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5107..5129)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5118..5134
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5142..5148,5149..5166,5167..5172))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5187..5208
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5325..5342)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5496..6084)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5576..5595)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6255..7046,7047..7115))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6878..6897
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7116..7220)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7295..7314)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7486..7865
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7867..8065
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             8083..8263
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8310..8435
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8928..9161
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9346..9390
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9688..9805
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(9862,1..18,19..41,42..60,61..83,84..102,103..125,
                     126..144,145..167,168..186,187..209,210..228,229..251,
                     252..270)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
ORIGIN
        1 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
       61 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt
      121 accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga
      181 tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
      241 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagctcgg tacccgggtc
      301 gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg aaccgtcaga
      361 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca
      421 gcctccgcgg ccccgaattc accggttcta gaatgggggg ctgcaggaaa tttggtgagc
      481 ggcctccacc taaacgactt actagggaag ctatgcgaaa ttatttaaaa gagcgagggg
      541 atcaaacagt acttattctt catgcaaaag ttgcacagaa gtcatatgga aatgaaaaaa
      601 ggtttttttg cccacctcct tgtgtatatc ttatgggcag cggatggaag aaaaaaaaag
      661 aacaaatgga acgcgatggt tgttctgaac aagagtctca accgtgtgca tttattggga
      721 taggaaatag tgaccaagaa atgcagcagc taaacttgga aggaaagaac tattgcacag
      781 ccaaaacatt gtatatatct gactcagaca agcgaaagca cttcatgttg tctgtaaaga
      841 tgttctatgg caacagtgat gacattggtg tgttcctcag caagcggata aaagtcatct
      901 ccaaaccttc caaaaagaag cagtcattga aaaatgctga cttatgcatt gcctcaggaa
      961 caaaggtggc tctgtttaat cgactacgat cccagacagt tagtaccaga tacttgcatg
     1021 tagaaggagg taattttcat gccagttcac agcagtgggg agcctttttt attcatctct
     1081 tggatgatga tgaatcagaa ggagaagaat tcacagtccg agatggctac atccattatg
     1141 gacaaacagt caaacttgtg tgctcagtta ctggcatggc actcccaaga ttgataatta
     1201 ggaaagttga taagcagacc gcattattgg atgcagatga tcctgtgtca caactccata
     1261 aatgtgcatt ttaccttaag gatacagaaa gaatgtattt gtgcctttct caagaaagaa
     1321 taattcaatt tcaggccact ccatgtccaa aagaaccaaa taaagagatg ataaatgatg
     1381 gcgcttcctg gacaatcatt agcacagata aggcagagta tacattttat gagggaatgg
     1441 gccctgtcct tgccccagtc actcctgtgc ctgtggtaga gagccttcag ttgaatggcg
     1501 gtggggacgt agcaatgctt gaacttacag gacagaattt cactccaaat ttacgagtgt
     1561 ggtttgggga tgtagaagct gaaactatgt acaggtgtgg agagagtatg ctctgtgtcg
     1621 tcccagacat ttctgcattc cgagaaggtt ggagatgggt ccggcaacca gtccaggttc
     1681 cagtaacttt ggtccgaaat gatggaatca tttattccac cagccttacc tttacctaca
     1741 caccagaacc agggccgcgg ccacattgca gtgcagcagg agcaatcctt cgagccaatt
     1801 caagccaggt gccccctaac gaatcaaaca caaacagcga gggaagttac acaaacgcca
     1861 gcacaaattc aaccagtgtc acatcatcta cagccacagt ggtatcctaa ttcgaagtaa
     1921 agatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg
     1981 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     2041 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     2101 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     2161 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     2221 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     2281 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     2341 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     2401 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     2461 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     2521 ctccccgcat cgataccgtc gacctcgaga cctagaaaaa catggagcaa tcacaagtag
     2581 caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt
     2641 gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga
     2701 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     2761 acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg attggcagaa
     2821 ctacacacca gggccaggga tcagatatcc actgaccttt ggatggtgct acaagctagt
     2881 accagttgag caagagaagg tagaagaagc caatgaagga gagaacaccc gcttgttaca
     2941 ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtattagagt ggaggtttga
     3001 cagccgccta gcatttcatc acatggcccg agagctgcat ccggactgta ctgggtctct
     3061 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     3121 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     3181 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagggcccg
     3241 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     3301 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     3361 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     3421 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     3481 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc
     3541 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     3601 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     3661 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     3721 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     3781 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     3841 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     3901 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     3961 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     4021 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     4081 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     4141 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     4201 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt
     4261 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc
     4321 ttgtatatcc attttcggat ctgatcagca cgtgttgaca attaatcatc ggcatagtat
     4381 atcggcatag tataatacga caaggtgagg aactaaacca tggccaagtt gaccagtgcc
     4441 gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac cgaccggctc
     4501 gggttctccc gggacttcgt ggaggacgac ttcgccggtg tggtccggga cgacgtgacc
     4561 ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg
     4621 gtgcgcggcc tggacgagct gtacgccgag tggtcggagg tcgtgtccac gaacttccgg
     4681 gacgcctccg ggccggccat gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc
     4741 ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga ctgacacgtg
     4801 ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     4861 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     4921 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     4981 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     5041 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     5101 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     5161 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     5221 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     5281 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     5341 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     5401 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     5461 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     5521 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     5581 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     5641 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     5701 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     5761 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     5821 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     5881 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     5941 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     6001 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     6061 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     6121 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     6181 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     6241 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     6301 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     6361 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     6421 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     6481 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     6541 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     6601 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     6661 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     6721 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     6781 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     6841 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     6901 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     6961 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     7021 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     7081 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     7141 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     7201 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg
     7261 ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     7321 taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa
     7381 tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta
     7441 ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga
     7501 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
     7561 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
     7621 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
     7681 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
     7741 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
     7801 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
     7861 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
     7921 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
     7981 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
     8041 tacggtggga ggtctatata agcagcgcgt tttgcctgta ctgggtctct ctggttagac
     8101 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     8161 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     8221 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
     8281 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
     8341 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
     8401 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
     8461 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
     8521 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
     8581 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
     8641 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
     8701 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
     8761 ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
     8821 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     8881 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
     8941 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
     9001 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
     9061 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
     9121 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
     9181 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
     9241 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
     9301 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
     9361 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     9421 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     9481 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
     9541 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
     9601 agagacagag acagatccat tcgattagtg aacggatcgg cactgcgtgc gccaattctg
     9661 cagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag
     9721 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
     9781 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg
     9841 gttagatctc gagtttacca ct
//
