LOCUS       sequence-416336-        9956 bp    DNA     circular SYN 15-APR-2025
DEFINITION  doxycycline-inducible expression of Human RORC in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9956)
  AUTHORS   Altman A, Henriques-Oliveira L, Kurochkin I, Ascic E, Halitzki E, 
            Rosa F, Pires C, Pereira C-F
  TITLE     Transcription Factor Blueprints Underlying Dendritic Cell Diversity
  JOURNAL   Exp Hematol. 2024 Aug. 137:104298
REFERENCE   2  (bases 1 to 9956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Exp 
            Hematol. 2024 Aug. 137:104298"
COMMENT     Sequence Label: pFUW-TetO-RORC
FEATURES             Location/Qualifiers
     source          1..9956
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        27..406
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        408..606
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             624..804
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    851..976
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1469..1702
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1887..1931
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2229..2346
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2403..2421,2422..2444,2445..2463,2464..2486,2487..2505,
                     2506..2528,2529..2547,2548..2570,2571..2589,2590..2612,
                     2613..2631,2632..2654,2655..2673)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2706..2744
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2741..2765
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    4437..5025
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4490..4510)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4908..4919)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5028..5044)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5029..5045)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5550..5730
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5756..5773)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5762..5986
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6032..6460
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6119..6138)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6329..6350
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6469..6489)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6474..6803
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6654..6789
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6716..6735
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6851..6898
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6917..7291
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7421..7542
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7458..7477)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7512..7531
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7591..7607)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7591..7607)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7604..7626)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7615..7631
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7639..7645,7646..7663,7664..7669))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7684..7705
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7822..7839)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7993..8581)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8073..8092)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8752..9543,9544..9612))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9375..9394
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9613..9717)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9792..9811)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
       61 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      121 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      181 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      241 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      301 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      361 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
      421 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
      481 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
      541 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      601 aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
      661 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
      721 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
      781 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
      841 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
      901 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
      961 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     1021 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     1081 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     1141 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     1201 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     1261 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     1321 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     1381 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     1441 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     1501 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     1561 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     1621 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     1681 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     1741 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     1801 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     1861 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     1921 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     1981 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     2041 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     2101 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
     2161 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
     2221 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     2281 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     2341 aattttcggg tttattacag ggacagcaga gatccagttt ggttagatct cgagtttacc
     2401 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     2461 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     2521 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
     2581 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
     2641 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagct cggtacccgg
     2701 gtcgaggtag gcgtgtacgg tgggaggcct atataagcag agctcgttta gtgaaccgtc
     2761 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
     2821 ccagcctccg cggccccgaa ttcaccggtt ctagaatgga cagggcccca cagagacagc
     2881 accgagcctc acgggagctg ctggctgcaa agaagaccca cacctcacaa attgaagtga
     2941 tcccttgcaa aatctgtggg gacaagtcgt ctgggatcca ctacggggtt atcacctgtg
     3001 aggggtgcaa gggcttcttc cgccggagcc agcgctgtaa cgcggcctac tcctgcaccc
     3061 gtcagcagaa ctgccccatc gaccgcacca gccgaaaccg atgccagcac tgccgcctgc
     3121 agaaatgcct ggcgctgggc atgtcccgag atgctgtcaa gttcggccgc atgtccaaga
     3181 agcagaggga cagcctgcat gcagaagtgc agaaacagct gcagcagcgg caacagcagc
     3241 aacaggaacc agtggtcaag acccctccag caggggccca aggagcagat accctcacct
     3301 acaccttggg gctcccagac gggcagctgc ccctgggctc ctcgcctgac ctgcctgagg
     3361 cttctgcctg tccccctggc ctcctgaaag cctcaggctc tgggccctca tattccaaca
     3421 acttggccaa ggcagggctc aatggggcct catgccacct tgaatacagc cctgagcggg
     3481 gcaaggctga gggcagagag agcttctata gcacaggcag ccagctgacc cctgaccgat
     3541 gtggacttcg ttttgaggaa cacaggcatc ctgggcttgg ggaactggga cagggcccag
     3601 acagctacgg cagccccagt ttccgcagca caccggaggc accctatgcc tccctgacag
     3661 agatagagca cctggtgcag agcgtctgca agtcctacag ggagacatgc cagctgcggc
     3721 tggaggacct gctgcggcag cgctccaaca tcttctcccg ggaggaagtg actggctacc
     3781 agaggaagtc catgtgggag atgtgggaac ggtgtgccca ccacctcacc gaggccattc
     3841 agtacgtggt ggagttcgcc aagaggctct caggctttat ggagctctgc cagaatgacc
     3901 agattgtgct tctcaaagca ggagcaatgg aagtggtgct ggttaggatg tgccgggcct
     3961 acaatgctga caaccgcacg gtcttttttg aaggcaaata cggtggcatg gagctgttcc
     4021 gagccttggg ctgcagcgag ctcatcagct ccatctttga cttctcccac tccctaagtg
     4081 ccttgcactt ttccgaggat gagattgccc tctacacagc ccttgttctc atcaatgccc
     4141 atcggccagg gctccaagag aaaaggaaag tagaacagct gcagtacaat ctggagctgg
     4201 cctttcatca tcatctctgc aagactcatc gccaaagcat cctggcaaag ctgccaccca
     4261 aggggaagct tcggagcctg tgtagccagc atgtggaaag gctgcagatc ttccagcacc
     4321 tccaccccat cgtggtccaa gccgctttcc ctccactcta caaggagctc ttcagcactg
     4381 aaaccgagtc acctgtgggg ctgtccaagt gattcgaaga tatcaagctt atcgataatc
     4441 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     4501 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     4561 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     4621 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     4681 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     4741 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     4801 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     4861 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     4921 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     4981 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac
     5041 ctcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg
     5101 attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg
     5161 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     5221 aggggggact ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga
     5281 tctaccacac acaaggctac ttccctgatt ggcagaacta cacaccaggg ccagggatca
     5341 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcaa gagaaggtag
     5401 aagaagccaa tgaaggagag aacacccgct tgttacaccc tgtgagcctg catgggatgg
     5461 atgacccgga gagagaagta ttagagtgga ggtttgacag ccgcctagca tttcatcaca
     5521 tggcccgaga gctgcatccg gactgtactg ggtctctctg gttagaccag atctgagcct
     5581 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     5641 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     5701 ccttttagtc agtgtggaaa atctctagca gggcccgttt aaacccgctg atcagcctcg
     5761 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     5821 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     5881 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     5941 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     6001 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
     6061 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     6121 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     6181 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     6241 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     6301 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     6361 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     6421 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
     6481 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     6541 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     6601 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     6661 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     6721 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
     6781 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
     6841 atcagcacgt gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa
     6901 ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga
     6961 cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcccggg acttcgtgga
     7021 ggacgacttc gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg cggtccagga
     7081 ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta
     7141 cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac gcctccgggc cggccatgac
     7201 cgagatcggc gagcagccgt gggggcggga gttcgccctg cgcgacccgg ccggcaactg
     7261 cgtgcacttc gtggccgagg agcaggactg acacgtgcta cgagatttcg attccaccgc
     7321 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     7381 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
     7441 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     7501 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
     7561 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     7621 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     7681 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     7741 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     7801 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     7861 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     7921 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     7981 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     8041 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     8101 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     8161 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     8221 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     8281 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     8341 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     8401 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     8461 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     8521 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     8581 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     8641 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     8701 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     8761 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     8821 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     8881 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     8941 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     9001 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     9061 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     9121 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     9181 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     9241 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     9301 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     9361 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     9421 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     9481 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     9541 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     9601 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     9661 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     9721 atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg
     9781 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct
     9841 tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc
     9901 ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcg
//
