LOCUS       sequence-416355-        5100 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Vector to amplify the LB-geneticin fragment for transformation into 
            TSI locus 1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5100)
  AUTHORS   Darino M, Urban M, Kaur N, Machado Wood A, Grimwade-Mann M, Smith D,
            Beacham A, Hammond-Kosack K
  TITLE     Identification and functional characterisation of a locus for target
            site integration in Fusarium graminearum.
  JOURNAL   Fungal Biol Biotechnol. 2024 Feb 26;11(1):2. doi: 
            10.1186/s40694-024-00171-8.
  PUBMED    38409036
REFERENCE   2  (bases 1 to 5100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal Biol
            Biotechnol."; date: "2024-02-26"; volume: "11(1):2. doi"; pages: " 
            10.1186/s40694-024-00171-8"
COMMENT     Sequence Label: pJET-LB-geneticin
FEATURES             Location/Qualifiers
     source          1..5100
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     305..324
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        305..323
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(join(2932..2938,2939..2956,2957..2962))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    2977..2998
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3118..3135)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3289..3877)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3369..3388)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4048..4839,4840..4908))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4671..4690
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4909..5013)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5081..5099
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gcccctgcag ccgaattata ttatttttgc caaataattt ttaacaaaag ctctgaagtc
       61 ttcttcattt aaattcttag atgatacttc atctggaaaa ttgtcccaat tagtagcatc
      121 acgctgtgag taagttctaa accatttttt tattgttgta ttatctctaa tcttactact
      181 cgatgagttt tcggtattat ctctattttt aacttggagc aggttccatt cattgttttt
      241 ttcatcatag tgaataaaat caactgcttt aacacttgtg cctgaacacc atatccatcc
      301 ggcgtaatac gactcactat agggagagcg gccgccagat cttccggatg gctcgagttt
      361 ttcagcaaga tatatctcga ggtctccttt aatcatggag ctgcatggcc attctgaatt
      421 ggtctaatat atccgtatca gcagttgaat cgttgggcat gttgccaaga ctctgcattc
      481 gctgtgttgg atcagctgaa atgtgatctc caagctgcga tagtgagatt atgggaacgc
      541 tcaagactat gcgatcggtg ccttattcct gtccttggaa attttcccca ggaagaagac
      601 ctcatgagcc aaaattggcc cgtagtgtta cttgacaaat ttgatgtcaa atgattggtc
      661 tttgacagat atggggtttt ctcgttgtga tatcggttga tcacgcaaac ctgggatggc
      721 gtcagttgac ttgcaagcca cttatgaata tcggcttcag actacgggta tttacagggt
      781 gatatccagc catctagcaa gggagcgcgt agatgcaagg gctatcgttt tgtgctatgt
      841 ggcaacttca atgcctaatc attgtgagtc gtgatgagta gttgagcagg tcttcagtgt
      901 ccaggtattg catggcatgg ttgtaaaagt cacggttcat caaacccgtt gccctgctca
      961 ttgaaagcct gtactgtaac cacaataact gtgcagacag acatgctagt aatcactcga
     1021 ctgcagtgtc tctcaatgtc ccacctagtt atccgacact catattgaaa tcagatgaag
     1081 atagagcaag gatgaggttc gtgatgaatc catataggat aaaaaccaag ccacttatcg
     1141 tgttctgtcg tcgggtcatg gaaaccggca gaagtgcaga atgacaactc agagacgagg
     1201 ctgttgtttg aaaccagagg gaatgagggc tgggccatac taacggtacc agttcaagga
     1261 agaaacagtg caattatctt tgcgaaccca ggggctggtg acggaatttt catagtcaag
     1321 ctatcagagt aaagaagagg agcatgtcaa agtacaatta gagacaaata tatagtcgcg
     1381 tggagccaag agcggattcc tcagtctcgt aggtcgcttg acgaccgttg atctgcttga
     1441 tctcgtctcc cgaaaatgaa aatagactct gctaagctat tcttctgctt cgccggagcc
     1501 tgaagggcgt actagggttg cgaggtccaa tgcattaatg cattgcagat gagctgtatc
     1561 tggaagaggt aaacccgaaa cgcgttttat tcttgttgac atggagctat taaatcacta
     1621 gaaggcactc tttgctgctt ggacaaatga acgtatctta tcgagatcct gaacaccatt
     1681 tgtctcaact ccggagctga catcgacacc aacgatctta tatccagatt cgtcaagctg
     1741 tttgatgatt tcagtaacgt taagtggcag ggtaccaatt tacagggaat gaaggtaaag
     1801 gttatctagg atccacctga tcagaagaat tcgtccaaca ggcggtaaaa agcgatgcgt
     1861 tgagagtccg gggcggcgat accgtagagc acgaggaaac gatcggccca ctcaccgccg
     1921 agctcctcag cgatatcgcg cgttgcgagt gcgatgtcct ggtagcgatc cgctacgcca
     1981 agacgaccgc aatcgatgaa acctgagaag cgaccgttct cgaccatgat gttgggaagg
     2041 catgcatcgc cgtgcgtgac gaccagatcc tcaccgtctg gcatgcgggc cttcagacgt
     2101 gcaaagagtt cggcgggcgc caagccctgg tgttcctcgt cgagatcatc ctggtccaca
     2161 agacctgcct ccatacgtgt tcgagcgcgt tcgatgcgat gcttcgcctg gtgatcaaac
     2221 gggcacgtgg ccggatcaag ggtgtgaagg cgacgcatcg cgtcggccat aatcgagact
     2281 ttttctgccg gggcgagatg cgaagacagc aggtcttgac ccggaacctc tcccaggagg
     2341 agccaatcac ggccagcctc tgtgaccacg tcgaggaccg cagcacacgg cacacccgtt
     2401 gttgccaacc aggagaggcg ggctgcctca tcctgcaact cgttcagggc gccggagagg
     2461 tctgtcttaa cgaacaggac tggtcggctc gagatatatc tttctagaag atctcctaca
     2521 atattctcag ctgccatgga aaatcgatgt tcttctttta ttctctcaag attttcaggc
     2581 tgtatattaa aacttatatt aagaactatg ctaaccacct catcaggaac cgttgtaggt
     2641 ggcgtgggtt ttcttggcaa tcgactctca tgaaaactac gagctaaata ttcaatatgt
     2701 tcctcttgac caactttatt ctgcattttt tttgaacgag gtttagagca agcttcagga
     2761 aactgagaca ggaattttat taaaaattta aattttgaag aaagttcagg gttaatagca
     2821 tccatttttt gctttgcaag ttcctcagca ttcttaacaa aagacgtctc ttttgacatg
     2881 tttaaagttt aaacctcctg tgtgaaatta ttatccgctc ataattccac acattatacg
     2941 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     3001 tgcgttgcgc tcactgccaa ttgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     3061 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     3121 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     3181 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     3241 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     3301 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     3361 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     3421 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     3481 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     3541 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     3601 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     3661 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     3721 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     3781 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     3841 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     3901 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     3961 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     4021 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     4081 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     4141 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     4201 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     4261 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     4321 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     4381 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     4441 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     4501 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     4561 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     4621 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     4681 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     4741 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     4801 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     4861 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     4921 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     4981 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     5041 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
//
