LOCUS       sequence-416758-       10486 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Mammalian expression of CRY2clust-mCherry-RANK-P2A-CIBNcaax 
            (Opto-RANKm).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10486)
  AUTHORS   Takada A, Asano T, Nakahama KI, Ono T, Nakata T, Ishii T
  TITLE     Development of an optogenetics tool, Opto-RANK, for control of 
            osteoclast differentiation using blue light.
  JOURNAL   Sci Rep. 2024 Jan 19;14(1):1749. doi: 10.1038/s41598-024-52056-w.
  PUBMED    38242937
REFERENCE   2  (bases 1 to 10486)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2024-01-19"; volume: "14(1):1749. doi"; pages: " 
            10.1038/s41598-024-52056-w"
COMMENT     Sequence Label: CRY2clust-mCherry-RANK-P2A-CIBNcaax
FEATURES             Location/Qualifiers
     source          1..10486
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        366..643
                     /label=chicken beta-actin promoter
     intron          644..1660
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1584..1606
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1668..1687
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             3310..4017
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3455..3473)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3723..3742
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             5263..5319
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    6547..7135
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6600..6620)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7018..7029)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    7274..7395
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7311..7330)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7365..7384
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(7402..7857)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7539..7560)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7751..7770
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7884..7988
                     /gene="bla"
                     /label=AmpR promoter
     promoter        7990..8347
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7990..8181
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8013..8033)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8198..8333
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8382..9176
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8382..9176
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8436..8455)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9046..9065
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9364..9383)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9408..9455
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9784..10372
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10273..10292
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc
      421 cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg
      481 gggggggcgc gcgccaggcg gggcggggcg gggcgagggg cggggcgggg cgaggcggag
      541 aggtgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg
      601 gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgcgctg
      661 ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac
      721 cgcgttactc ccacaggtga gcgggcggga cggcccttct cctccgggct gtaattagcg
      781 cttggtttaa tgacggcttg tttcttttct gtggctgcgt gaaagccttg aggggctccg
      841 ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg
      901 gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg
      961 gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc gggggcggtg ccccgcggtg
     1021 cggggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc
     1081 agggggtgtg ggcgcgtcgg tcgggctgca accccccctg cacccccctc cccgagttgc
     1141 tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg cgtggcgcgg ggctcgccgt
     1201 gccgggcggg gggtggcggc aggtgggggt gccgggcggg gcggggccgc ctcgggccgg
     1261 ggagggctcg ggggaggggc gcggcggccc ccggagcgcc ggcggctgtc gaggcgcggc
     1321 gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc
     1381 ccaaatctgt gcggagccga aatctgggag gcgccgccgc accccctcta gcgggcgcgg
     1441 ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc
     1501 gcgccgccgt ccccttctcc ctctccagcc tcggggctgt ccgcgggggg acggctgcct
     1561 tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg gctctagagc
     1621 ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt
     1681 tattgtgctg tctcatcatt ttggcaaaga attgatttat cgctagttaa ttaagctagc
     1741 gccaccatga agatggacaa aaagaccatc gtctggtttc ggagagattt gagaatagaa
     1801 gataatcccg cgctcgccgc cgcggcccac gagggttccg tcttccccgt tttcatttgg
     1861 tgtcctgaag aagaaggcca gttttatccc ggaagggcct ctaggtggtg gatgaagcaa
     1921 agtctggccc atcttagcca gtcactgaaa gcactgggca gtgatcttac cctgatcaag
     1981 acacacaata ccatctctgc catcctcgac tgcatcaggg tgaccggcgc aacgaaagtc
     2041 gtgtttaacc acctgtacga tccagttagt ctggtgcgcg accacactgt gaaggagaag
     2101 ctggtggaac gggggatcag tgtgcagagc tacaacgggg accttctgta cgagccatgg
     2161 gagatctatt gcgagaaagg gaaaccgttc acctccttca acagttactg gaagaaatgt
     2221 ttggatatgt caatagagtc cgttatgttg ccccctccct ggagactgat gccgattact
     2281 gctgctgcag aggccatctg ggcctgctcc atcgaggaac tcggtctgga aaatgaagca
     2341 gaaaagccaa gcaatgcact tctcactaga gcctggagcc ccggctggtc taatgccgac
     2401 aagctgctta acgagttcat cgaaaaacaa ctgattgact acgcgaagaa ctccaagaaa
     2461 gtggtaggta actcaactag cttgctctct ccatatctcc attttggcga gatttctgtc
     2521 cgccatgtat ttcagtgcgc tcggatgaaa cagattatct gggctcgcga taaaaacagc
     2581 gaaggcgaag aaagcgccga tctgttcctg cgagggatcg gacttcggga atactcccgg
     2641 tatatatgtt tcaactttcc attcacacac gagcagagtc tgttgtccca cctcaggttc
     2701 ttcccctggg acgccgatgt cgacaaattc aaggcatgga gacagggaag gacaggatac
     2761 ccactcgtgg atgctggcat gagagagctc tgggctacag gctggatgca caaccgcatc
     2821 cgggtaatcg tgtcctcatt tgctgtcaag tttctgctcc tgccttggaa atggggaatg
     2881 aagtactttt gggataccct tctcgacgcc gacttggagt gtgacattct gggatggcaa
     2941 tatattagcg ggtcaattcc tgacggccat gagttggaca ggttggacaa tccggccttg
     3001 cagggagcta agtatgatcc cgaaggagag tatattcgac agtggctccc cgagctggcc
     3061 cgacttccta cggagtggat tcaccatcct tgggacgcac cactgacagt gctcaaggca
     3121 agcggggtgg agctgggcac caattacgct aagcctatag ttgatataga tacagcacgc
     3181 gagctgctgg ctaaagcgat ctctcgcact cgggaggcgc agattatgat cggtgctgcc
     3241 gcccgggatc caccggatct agataacgaa ttcgatagtg ctggtagtgc tggtagtgct
     3301 ggtactagta tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg
     3361 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
     3421 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc
     3481 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac
     3541 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag
     3601 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
     3661 ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac
     3721 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc
     3781 gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac
     3841 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc
     3901 tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa
     3961 cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtcc
     4021 ggactcagat ctcgagtggg tggatctgga ggttcatact acaggaaggg agggaaagcg
     4081 ctgacagcta atttgtggaa ttgggtcaat gatgcttgca gtagtctaag tggaaataag
     4141 gagtcctcag gggaccgttg tgctggttcc cactcggcaa cctccagtca gcaagaagtg
     4201 tgtgaaggta tcttactaat gactcgggag gagaagatgg ttccagaaga cggtgctgga
     4261 gtctgtgggc ctgtgtgtgc ggcaggtggg ccctgggcag aagtcagaga ttctaggacg
     4321 ttcacactgg tcagcgaggt tgagacgcaa ggagacctgt cgaggaagat tcccacagag
     4381 gatgagtaca cggaccggcc ctcgcagcct tcgactggtt cactgctcct aatccagcag
     4441 ggaagcaaat ctataccccc attccaggag cccctggaag tgggggagaa cgacagttta
     4501 agccagtgtt tcaccgggac tgaaagcacg gtggattctg agggctgtga cttcactgag
     4561 cctccgagca gaactgactc tatgcccgtg tcccctgaaa agcacctgac aaaagaaata
     4621 gaaggtgaca gttgcctccc ctgggtggtc agctccaact caacagatgg ctacacaggc
     4681 agtgggaaca ctcctgggga ggaccatgaa ccctttccag ggtccctgaa atgtggacca
     4741 ttgccccagt gtgcctacag catgggcttt cccagtgaag cagcagccag catggcagag
     4801 gcgggagtac ggccccagga cagggctgat gagaggggag cctcagggtc cgggagctcc
     4861 cccagtgacc agccacctgc ctctgggaac gtgactggaa acagtaactc cacgttcatc
     4921 tctagcgggc aggtgatgaa cttcaagggt gacatcatcg tggtgtatgt cagccagacc
     4981 tcgcaggagg gcccgggttc cgcagagccc gagtcggagc ccgtgggccg ccctgtgcag
     5041 gaggagacgc tggcacacag agactccttt gcgggcaccg cgccgcgctt ccccgacgtc
     5101 tgtgccaccg gggctgggct gcaggagcag ggggcacccc ggcagaagga cgggacatcg
     5161 cggccggtgc aggagcaggg tggggcgcag acttcactcc atacccaggg gtccggacaa
     5221 tgtgcagaag gaggttcagg tggatctggt accggaagcg gagctactaa cttcagcctg
     5281 ctgaagcagg ctggagacgt ggaggagaac cctggacctg ctccaggatc cgactctgct
     5341 ggctcagccg gaatgaacgg ggctatagga ggcgacctct tgttgaactt tcctgacatg
     5401 tcagtcttgg aacgacagcg cgcccacctg aagtacttga atcccacctt cgatagccca
     5461 ctggctggct ttttcgccga ttcatcaatg atcacagggg gagaaatgga ttcctacctc
     5521 agcactgcag gactgaatct gccgatgatg tacggagaaa cgactgtgga gggcgattcc
     5581 cgcctgtcca ttagcccaga aaccactctg ggcaccggga actttaaggc tgccaaattt
     5641 gatactgaaa ctaaggattg caacgaagcc gcaaaaaaaa tgactatgaa ccgggatgat
     5701 ctggtggagg agggtgagga ggagaaaagc aaaataacag agcaaaacaa tggcagcacc
     5761 aagagcatca agaaaatgaa gcataaggcc aagaaagaag aaaacaactt cagtaatgat
     5821 tctagcaaag tgacaaaaga gctcgaaaaa accgattaca tcgccccggg ttctgatagc
     5881 gcaggcagtg ctggtatgaa cggagctatt ggaggagatc tgttgctgaa cttccctgat
     5941 atgtctgtcc ttgagcgaca acgcgcacac ctcaagtatc tgaatcctac ctttgacagc
     6001 cctctggccg gattctttgc cgactcatcc atgattacag ggggcgaaat ggactcctat
     6061 ctcagcacag ccgggctcaa cctccccatg atgtatggcg agacgaccgt cgaaggcgac
     6121 tctaggctca gcatcagccc ggaaactacc ttgggaacag ggaacttcaa ggctgccaag
     6181 ttcgacaccg aaacaaaaga ctgcaatgaa gctgccaaga agatgacaat gaatagggac
     6241 gatttggtcg aggaaggaga agaagagaag agcaagatta cagaacaaaa taacggctcc
     6301 actaagtcca tcaaaaagat gaagcacaaa gccaaaaagg aggagaataa cttcagcaac
     6361 gatagctcca aggtgaccaa ggagctggaa aagaccgact atatcaccgg tggaggctct
     6421 tctgctggtg gttcagctgg aggaagtgct ggtggtaaaa agaagaaaaa gaagtcaaag
     6481 acaaagtgtg taattatgta agcggccgca ctagtttgag tcgacggcgc gccatagctt
     6541 atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     6601 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     6661 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     6721 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     6781 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     6841 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     6901 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     6961 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     7021 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     7081 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga
     7141 taccgtggat ccaaggccgc gactctagat cataatcagc cataccacat ttgtagaggt
     7201 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
     7261 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     7321 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     7381 catcaatgta tcttaaggcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     7441 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     7501 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     7561 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     7621 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     7681 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     7741 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     7801 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag
     7861 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     7921 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     7981 ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa
     8041 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     8101 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     8161 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     8221 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     8281 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     8341 ttgcaaagat cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat
     8401 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     8461 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     8521 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc
     8581 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     8641 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     8701 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     8761 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     8821 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     8881 cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga
     8941 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     9001 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     9061 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     9121 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg
     9181 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga
     9241 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
     9301 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct
     9361 aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag
     9421 acagaataaa acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg
     9481 ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc
     9541 cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg
     9601 ggcggcaggc cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact
     9661 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     9721 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     9781 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     9841 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     9901 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     9961 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
    10021 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
    10081 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
    10141 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
    10201 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
    10261 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
    10321 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
    10381 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
    10441 tgcgttatcc cctgattctg tggataaccg tattaccgcc atgcat
//
