LOCUS       sequence-41677-e         741 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Zhao B, Li L, Tumaneng K, Wang CY, Guan KL
  TITLE     A coordinated phosphorylation by Lats and CK1 regulates YAP 
            stability through SCF(beta-TRCP).
  JOURNAL   Genes Dev. 2010 Jan 1. 24(1):72-85.
  PUBMED    20048001
REFERENCE   2  (bases 1 to 741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2010 Jan 1. 24(1):72-85."
COMMENT     Sequence Label: pCMV-Flag-YAP-4SA(S127)
FEATURES             Location/Qualifiers
     source          1..741
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             38..61
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      86..95
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             95..118
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gagctcgttt agtgaccgtc agaattgatc taccatggac tacaaagacg atgacgacaa
       61 gcttgcggcc gcgaattcaa gcttagccac catggactac aaagacgatg acgataaagc
      121 aaggctcgaa tcggtaccta aggatcccgg gcagcagccg ccgcctcaac cggcccccca
      181 gggccaaggg cagccgcctt cgcagccccc gcaggggcag ggcccgccgt ccggacccgg
      241 gcaaccggca cccgcggcga cccaggcggc gccgcaggca ccccccgccg ggcatcagat
      301 cgtgcacgtc cgcggggacg cggagaccga cctggaggcg ctcttcaacg ccgtcatgaa
      361 ccccaagacg gccaacgtgc cccagaccgt gcccatgagg ctccggaagc tgcccgactc
      421 cttcttcaag ccgccggagc ccaaatccca ctcccgacag gccgctactg atgcaggcac
      481 tgcaggagcc ctgactccac agcatgttcg agctcattcc tctccagctt ctctgcagtt
      541 gggagctgtt tctcctggga cactgacccc cactggagta gtctctggcc cagcagctac
      601 acccacagct cagcatcttc gacaggctgc ttttgagata cctgatgatg tacctctgcc
      661 agcaggttgg gagatggcaa agacatcttc tggtcagaga tacttcttaa atcacatcga
      721 tcagacaaca acatggcagg a
//
