LOCUS       sequence-416807-       12069 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expresses SM(FLAG)-optimal Firefly luciferase, and EMCVIRES-driven 
            Renilla luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12069)
  AUTHORS   Barrington CL, Galindo G, Koch AL, Horton ER, Morrison EJ, Tisa S, 
            Stasevich TJ, Rissland OS
  TITLE     Synonymous codon usage regulates translation initiation.
  JOURNAL   Cell Rep. 2023 Dec 26;42(12):113413. doi: 
            10.1016/j.celrep.2023.113413. Epub 2023 Dec 12.
  PUBMED    38096059
REFERENCE   2  (bases 1 to 12069)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2023-12-26"; volume: "42(12):113413. doi"; pages: " 
            10.1016/j.celrep.2023.113413. Epub 2023 Dec 12"
COMMENT     Sequence Label: pUB_SM-FLuc100_EMCV-Ren
FEATURES             Location/Qualifiers
     source          1..12069
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2218..2616
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     regulatory      2622..2631
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2631..2654
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2658..2681
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2685..2708
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3213..3236
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3243..3266
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3279..3302
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3309..3332
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3525..3548
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3552..3575
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3579..3602
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    5309..5771
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5365..5382)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5592..5611
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     misc_feature    8459..9047
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8512..8532)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8930..8941)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9122..9355
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9474..9492)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9560..9664
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9665..9733,9734..10525)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9883..9902)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10696..11284
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11185..11204
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11438..11455
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11695..11716
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11731..11736,11737..11754,11755..11761)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11769..11785
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11774..11796
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11793..11809
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11793..11809
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11859..11878
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtggc
     2221 ctccgcgccg ggttttggcg cctcccgcgg gcgcccccct cctcacggcg agcgctgcca
     2281 cgtcagacga agggcgcagc gagcgtcctg atccttccgc ccggacgctc aggacagcgg
     2341 cccgctgctc ataagactcg gccttagaac cccagtatca gcagaaggac attttaggac
     2401 gggacttggg tgactctagg gcactggttt tctttccaga gagcggaaca ggcgaggaaa
     2461 agtagtccct tctcggcgat tctgcggagg gatctccgtg gggcggtgaa cgccgatgat
     2521 tatataagga cgcgccgggt gtggcacagc tagttccgtc gcagccggga tttgggtcgc
     2581 ggttcttgtt tgtggatcgc tgtgatcgtc acttggcggc cgccaccatg gactacaagg
     2641 acgacgacga caaaggtgac tacaaagatg atgacgataa aggcgactat aaggacgatg
     2701 acgacaaggg cggaaactca ctgatcaagg aaaacatgcg gatgaaggtg gtgatggagg
     2761 gctccgtgaa tggtcaccag ttcaagtgca ccggagaggg agagggaaac ccgtacatgg
     2821 gaactcagac catgcgcatt aaggtcatcg aaggaggtcc gctgccgttc gctttcgata
     2881 tcctggccac ttcgttcgga ggagggtcgc gcacgttcat caagtacccg aagggaatcc
     2941 cggacttctt taagcagtca ttcccggaag gattcacttg ggaacgggtg acccggtatg
     3001 aagatggagg tgtggtgact gtcatgcaag atacttcgct ggaggatggg tgcctcgtgt
     3061 accacgtcca agtccgcgga gtgaatttcc cgtccaacgg accagtgatg cagaaaaaga
     3121 cgaagggttg ggaacctaat actgaaatga tgtaccccgc agacggaggg ctgaggggct
     3181 acacccacat ggcgctgaag gtcgacggag gagattacaa ggatgacgac gataagcaac
     3241 aagattacaa agacgatgat gacaagggcc agcagggcga ctacaaggac gacgacgaca
     3301 agcagcagga ctacaaagat gacgatgata aaggaggagg acatctgtcc tgttcgttcg
     3361 tgaccaccta cagatcaaag aaaaccgtgg gaaacatcaa gatgccgggc attcatgccg
     3421 tcgaccaccg cctggagcgg ctcgaagaat cagacaatga gatgttcgtc gtgcaaagag
     3481 aacatgccgt ggccaagttc gcgggactgg gaggcggtgg aggcgattac aaagacgatg
     3541 atgacaaggg tgactataaa gacgacgatg acaaagggga ttacaaggat gatgatgata
     3601 agggaggcgg tggatcaggt ggaggaggtt cactgcagga ggacgccaaa aacattaaaa
     3661 aaggcccggc tccattctac ccgctggagg acggtactgc tggcgagcag ctgcacaaag
     3721 ctatgaaacg atacgcacta gttccgggta ctattgcttt cactgacgct cacatcgagg
     3781 tggacatcac ttacgctgag tacttcgaga tgagcgttcg gttggccgag gctatgaaac
     3841 gatacggcct gaacactaac caccgaatcg tggtgtgctc agagaactca ttacagttct
     3901 tcatgccggt gttgggcgcc ttattcatcg gcgttgctgt tgccccagcc aacgacattt
     3961 acaacgagcg agagttgtta aactcaatgg gcatttcaca gccaactgtg gtgttcgttt
     4021 caaaaaaagg cttgcagaaa attttgaacg tgcagaaaaa attaccaatc atccagaaaa
     4081 ttattatcat ggacagcaaa actgactacc agggtttcca gagcatgtac actttcgtta
     4141 cttcacactt gccaccgggt ttcaacgagt acgacttcgt gccagagagc ttcgaccgag
     4201 acaaaactat tgccctgatc atgaactcat caggctcaac tggtctgccg aaaggtgttg
     4261 ctctgccgca ccggactgcc tgcgtgcgat tctcacacgc ccgggaccca attttcggca
     4321 accagatcat tccggacact gccattttaa gcgttgttcc attccaccac ggtttcggca
     4381 tgttcactac tctgggttac ttcatatgcg gtttccgagt tgtgttaatg taccggttcg
     4441 aggaggagct gttcctgcga agcttgcagg actacaaaat tcagtcagct ctgctggtgc
     4501 caactctgtt cagcttcttc gccaaaagca ctctgattga caaatacgac ttatcaaact
     4561 tacacgagat tgcttcaggt ggcgctccgt tatcaaaaga ggttggcgag gccgttgcca
     4621 aacgattcca cctgccaggt atccgacagg gttacggttt gactgagact acttcagcta
     4681 ttctgattac tccggagggt gacgacaaac cgggcgctgt gggtaaagtt gttccattct
     4741 tcgaggctaa agttgtggac ctggacactg gtaaaactct gggcgttaac cagcgaggcg
     4801 agctgtgcgt gcgaggtccg atgattatga gcggttacgt gaacaacccg gaggctacta
     4861 acgccttgat tgacaaagac ggttggttac acagcggtga catcgcttac tgggacgagg
     4921 acgagcactt cttcatcgtt gaccggctga aatcactgat taaatacaaa ggctaccagg
     4981 tggctccggc tgagttggag tcaatcttgt tacagcaccc aaacatcttc gacgctggtg
     5041 tggctggtct gccggacgac gacgccggtg agttaccagc cgccgttgtt gttttggagc
     5101 acggtaaaac tatgactgag aaagagatcg tggactacgt tgccagccag gttactactg
     5161 ctaaaaaatt gcggggtggc gttgtgttcg tcgacgaggt tccgaaaggt ttaactggca
     5221 aattggacgc tcgaaaaatc cgagagatcc tgatcaaagc caaaaaaggc ggcaaaatcg
     5281 ccgtgtaagg agacccaagc tggctagcga gggcccggaa acctggccct gtcttcttga
     5341 cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg
     5401 tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt
     5461 gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat
     5521 aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg
     5581 aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg
     5641 taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt
     5701 cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa
     5761 cacgatgata agctagcgcg gccgccacca tgaccagcaa ggtgtacgac cccgagcagc
     5821 gcaagcgcat gatcaccggc ccccagtggt gggcccgctg caagcagatg aacgtgctgg
     5881 acagcttcat caactactac gacagcgaga agcacgccga gaacgccgtg atcttcctgc
     5941 acggcaacgc cgccagcagc tacctgtggc gccacgtggt gccccacatc gagcccgtgg
     6001 cccgctgcat catccccgac ctgatcggca tgggcaagag cggcaagagc ggcaacggca
     6061 gctaccgcct gctggaccac tacaagtacc tgaccgcctg gttcgagctg ctgaacctgc
     6121 ccaagaagat catcttcgtg ggccacgact ggggcgcctg cctggccttc cactacagct
     6181 acgagcacca ggacaagatc aaggccatcg tgcacgccga gagcgtggtg gacgtgatcg
     6241 agagctggga cgagtggccc gacatcgagg aggacatcgc cctgatcaag agcgaggagg
     6301 gcgagaagat ggtgctggag aacaacttct tcgtggagac catgctgccc agcaagatca
     6361 tgcgcaagct ggagcccgag gagttcgccg cctacctgga gcccttcaag gagaagggcg
     6421 aggtgcgccg ccccaccctg agctggcccc gcgagatccc cctggtgaag ggcggcaagc
     6481 ccgacgtggt gcagatcgtg cgcaactaca acgcctacct gcgcgccagc gacgacctgc
     6541 ccaagatgtt catcgagagc gaccccggct tcttcagcaa cgccatcgtg gagggcgcca
     6601 agaagttccc caacaccgag ttcgtgaagg tgaagggcct gcacttcagc caggaggacg
     6661 cccccgacga gatgggcaag tacatcaaga gcttcgtgga gcgcgtgctg aagaacgagc
     6721 agtaactagg cggactgtta ctgagctgcg ttttacaccc tttctttgac aaaacctaac
     6781 ttgcgcagaa aaaaaaaaaa taagagacaa cattggcatg gctttgtttt tttaaatttt
     6841 ttttaaagtt tttttttttt tttttttttt ttaagttttt ttgttttgtt ttggcgcttt
     6901 tgactcagga tttaaaaact ggaacggtga aggcgacagc agttggttgg agcaaacatc
     6961 ccccaaagtt ctacaaatgt ggctgaggac tttgtacatt gttttgtttt tttttttttt
     7021 ggttttgtct ttttttaata gtcattccaa gtatccatga aataagtggt tacaggaagt
     7081 ccctcaccct cccaaaagcc acccccactc ctaagaggag gatggtcgcg tccatgccct
     7141 gagtccaccc cggggaaggt gacaaccggt aacctacaaa cgggtggagg atcaccccac
     7201 ccgacacttc acaatcaagg ggtacaatac acaagggtgg aggaacaccc caccctccag
     7261 acacattaca cagaaatcca atcaaacaga agcaccatca gggcttctgc taccaaattt
     7321 atctcaaaaa actacaacaa ggaatcacca tcagggattc cctgtgcaat atacgtcaaa
     7381 cgagggccac gacgggagga cgatcacgcc tcccgaatat cggcatgtct ggctttcgaa
     7441 ttcagtgcgt ggagcatcag cccacgcagc caatcagagt cgaatacaag tcgactttcg
     7501 cgaagagcat cagccttcgc gccattctta cacaaaccac actctcccct acaggaacag
     7561 catcagcgtt cctgcccagt acccaactca agaaaattta tgtccccatg cagcatcagc
     7621 gcatgggccc caagaataca tccccaacaa aatcacatcc gagcaccaac agggctcgga
     7681 gtgttgtttc ttgtccaact ggacaaaccc tccatggacc atcaggccat ggactctcac
     7741 caacaagaca aaaactactc ttctcgaagc agcatcagcg cttcgaaaca ctcgagcata
     7801 cattgtgcct atttcttggg tggacgatca cgccacccat gctctcacga atttcaaaac
     7861 acggacaagg acgagcacca ccagggctcg tcgttccacg tccaatacga ttacttacct
     7921 ttcgggatca cgatcacgga tcccgcagct acatcacttc cactcaggac attcaagcat
     7981 gcacgatcac ggcatgctcc acaagtctca accacagaaa ctaccaaatg ggttcagcac
     8041 cagcgaaccc actcctacct caaacctctt cccacaaaac tggcaagcag gatcaccgct
     8101 tgcccattcc aacataccaa atcaaaaaca attactggta cagcatcagc gtaccagccc
     8161 acatctctca ctactatcaa aaaccaaacc gttcagcaac agcgaacggt acacacggaa
     8221 aaatcaactg gtttacaaat acgaaagacg atcacgcttt cgtccagcgc aaactattac
     8281 gaaaaacatc cgacgggaag agcaacagcc ttcccgcggc ggaaaacctc acaaaaacac
     8341 gacaaacgga tgcacgaaca cggcatccgc cgacaaccca caaacttaca accaggcaaa
     8401 cggtgcagga tcaccgcacc gtacatcaaa cacctcagat ctcatatcga tagatcctaa
     8461 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     8521 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     8581 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     8641 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     8701 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     8761 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     8821 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     8881 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     8941 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     9001 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg agatccttta
     9061 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     9121 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     9181 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     9241 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     9301 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     9361 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     9421 gagaggcccg ggttaattaa ggaaagggct agatcattct tgaagacgaa agggcctcgt
     9481 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     9541 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     9601 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     9661 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     9721 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     9781 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     9841 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     9901 atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     9961 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
    10021 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
    10081 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
    10141 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
    10201 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
    10261 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
    10321 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
    10381 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
    10441 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
    10501 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
    10561 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
    10621 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
    10681 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
    10741 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
    10801 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
    10861 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
    10921 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
    10981 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
    11041 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
    11101 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
    11161 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
    11221 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
    11281 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
    11341 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
    11401 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
    11461 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
    11521 caagctcatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
    11581 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
    11641 cccgtggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
    11701 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
    11761 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa
    11821 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
    11881 tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact
    11941 tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg
    12001 tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa
    12061 cttagtagt
//
