LOCUS       sequence-417063-       12653 bp    DNA     circular SYN 15-APR-2025
DEFINITION  doxycycline-inducible expression of Mouse Stat1in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12653)
  AUTHORS   Altman A, Henriques-Oliveira L, Kurochkin I, Ascic E, Halitzki E, 
            Rosa F, Pires C, Pereira C-F
  TITLE     Transcription Factor Blueprints Underlying Dendritic Cell Diversity
  JOURNAL   Exp Hematol. 2024 Aug. 137:104298
REFERENCE   2  (bases 1 to 12653)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Exp 
            Hematol. 2024 Aug. 137:104298"
COMMENT     Sequence Label: pFUW-TetO-Stat1
FEATURES             Location/Qualifiers
     source          1..12653
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        27..406
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        408..606
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             624..804
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    851..976
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1469..1702
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1887..1931
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2229..2346
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2403..2421,2422..2444,2445..2463,2464..2486,2487..2505,
                     2506..2528,2529..2547,2548..2570,2571..2589,2590..2612,
                     2613..2631,2632..2654,2655..2673)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2706..2744
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2741..2765
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    complement(5259..5283)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(5284..5303)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5285..5303)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5314..5331)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5314..5330)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5323..5345)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     polyA_signal    5735..5869
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      5995..6449
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     misc_feature    7134..7722
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7187..7207)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7605..7616)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7725..7741)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7726..7742)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8247..8427
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8453..8470)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8459..8683
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8729..9157
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9166..9186)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9171..9500
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9351..9486
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9413..9432
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9548..9595
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9614..9988
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10118..10239
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10288..10304)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10288..10304)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10301..10323)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10312..10328
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10336..10342,10343..10360,10361..10366))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10381..10402
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10519..10536)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10690..11278)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10770..10789)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11449..12240,12241..12309))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(12310..12414)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(12489..12508)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
       61 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      121 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      181 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      241 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      301 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      361 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
      421 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
      481 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
      541 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      601 aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
      661 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
      721 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
      781 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
      841 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
      901 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
      961 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     1021 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     1081 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     1141 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     1201 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     1261 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     1321 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     1381 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     1441 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     1501 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     1561 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     1621 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     1681 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     1741 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     1801 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     1861 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     1921 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     1981 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     2041 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     2101 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
     2161 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
     2221 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     2281 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     2341 aattttcggg tttattacag ggacagcaga gatccagttt ggttagatct cgagtttacc
     2401 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     2461 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     2521 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
     2581 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
     2641 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagct cggtacccgg
     2701 gtcgaggtag gcgtgtacgg tgggaggcct atataagcag agctcgttta gtgaaccgtc
     2761 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
     2821 ccagcctccg cggccccgaa ttcaccggtt ctagaatgtc acagtggttc gagcttcagc
     2881 agctggactc caagttcctg gagcaggtcc accagctgta cgatgacagt ttccccatgg
     2941 aaatcagaca gtacctggcc cagtggctgg aaaagcaaga ctgggagcac gctgcctatg
     3001 atgtctcgtt tgcgaccatc cgcttccacg acctcctctc gcagctggac gaccagtaca
     3061 gccgcttttc tctggagaat aatttcttgt tgcagcacaa catacggaaa agcaagcgta
     3121 atctccagga taacttccaa gaagatcccg tacagatgtc catgatcatc tacaactgtc
     3181 tgaaggaaga aaggaagatt ttggaaaatg cccaaagatt taatcaggcc caggagggaa
     3241 atattcagaa cactgtgatg ttagataaac agaaggagct ggacagtaaa gtcagaaatg
     3301 tgaaggatca agtcatgtgc atagagcagg aaatcaagac cctagaagaa ttacaagatg
     3361 aatatgactt taaatgcaaa acctctcaga acagagaagg tgaagccaat ggtgtggcga
     3421 agagcgacca aaaacaggaa cagctgctgc tccacaagat gtttttaatg cttgacaata
     3481 agagaaagga gataattcac aaaatcagag agttgctgaa ttccatcgag ctcactcaga
     3541 acactctgat taatgacgag ctcgtggagt ggaagcgaag gcagcagagc gcctgcatcg
     3601 ggggaccgcc caacgcctgc ctggatcagc tgcaaagctg gttcaccatt gttgcagaga
     3661 ccctgcagca gatccgtcag cagcttaaaa agctggagga gttggaacag aaattcacct
     3721 atgagcccga ccctattaca aaaaacaagc aggtgttgtc agatcgaacc ttcctcctct
     3781 tccagcagct cattcagagc tccttcgtgg tagaacgaca gccgtgcatg cccactcacc
     3841 cgcagaggcc cctggtcttg aagactgggg tacagttcac tgtcaagctg agactgttgg
     3901 tgaaattgca agagctgaac tataacttga aagtgaaagt ctcatttgac aaagatgtga
     3961 acgagaaaaa cacagtaaaa ggatttcgga agttcaacat cttgggtacg cacacaaaag
     4021 tgatgaacat ggaagaatcc accaacggaa gtctggcagc tgagttccga cacctgcaac
     4081 tgaaggaaca gaaaaacgct gggaacagaa ctaatgaggg gcctctcatt gtcaccgaag
     4141 aacttcactc tcttagcttt gaaacccagt tgtgccagcc aggcttggtg attgacctgg
     4201 agaccacctc tcttcctgtc gtggtgatct ccaacgtcag ccagctcccc agtggctggg
     4261 cgtctatcct gtggtacaac atgctggtga cagagcccag gaatctctcc ttcttcctga
     4321 atcccccgtg cgcgtggtgg tcccagctct cagaggtgtt gagttggcag ttttcatcag
     4381 tcaccaagag aggtctgaac gcagaccagc tgagcatgct gggagagaag ctgctgggcc
     4441 ctaatgctgg ccctgatggt cttattccat ggacaaggtt ttgtaaggaa aatattaatg
     4501 ataaaaattt ctccttctgg ccttggattg acaccatcct agagctcatt aagaagcacc
     4561 tgctgtgcct ctggaatgat gggtgcatta tgggcttcat cagcaaggag cgagaacgcg
     4621 ctctgctcaa ggaccagcag ccagggacgt tcctgcttag gttcagtgag agctcccggg
     4681 aaggggccat cacattcaca tgggtggaac ggtcccagaa cggaggtgaa cctgacttcc
     4741 atgctgtgga gccctacacg aaaaaagagc tttcagctgt tactttccca gatattattc
     4801 gcaactacaa agtcatggct gccgagaaca taccagagaa tcccctgaag tatctgtacc
     4861 ccaatattga caaagaccac gcctttggga agtattattc cagaccaaag gaagcaccag
     4921 aaccgatgga gcttgacgac cctaagcgaa ctggatacat caagactgag ttgatttctg
     4981 tgtctgaagt gtaagtgagc acagaagact ggcttactgg cttgttcagg accgcaggcc
     5041 agccccgtcc ctggctgcgg tccattgatt gcactggctc atggctcatg gcttgctttc
     5101 ccaatgtaac tgatgttgcc accacagttg gacattctgg aaagtttgta actagtcctg
     5161 gtgtcaatgc ttactggcat tttattgcca tgagataaan aaaaaaaaaa aagggcggcc
     5221 gctctagagt atccctcgag gggcccaagc ttacgcgtac ccagctttct tgtacaaagt
     5281 ggtccctata gtgagtcgta ttataagcta ggcactggcc gtcgttttac aacgtcgtga
     5341 ctgggaaaac tgctagcttg ggatctttgt gaaggaacct tacttctgtg gtgtgacata
     5401 attggacaaa ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt
     5461 ataatgtgtt aaactagctg catatgcttg ctgcttgaga gttttgctta ctgagtatga
     5521 tttatgaaaa tattatacac aggagctagt gattctaatt gtttgtgtat tttagattca
     5581 cagtcccaag gctcatttca ggcccctcag tcctcacagt ctgttcatga tcataatcag
     5641 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
     5701 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     5761 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     5821 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcg atcctgcatt
     5881 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tggctggcgt aatagcgaag
     5941 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc
     6001 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     6061 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     6121 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     6181 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     6241 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttcttaata gtggactctt
     6301 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     6361 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     6421 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga
     6481 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gccaggtctt
     6541 ggactggtga gaacggcttg ctcggcagct tcgatgtgtg ctggagggag aataaaggtc
     6601 taagatgtgc gatagaggga agtcgcattg aattatgtgc tgtgtaggga tcgctggtat
     6661 caaatatgtg tgcccacccc tggcatgaga caataaccct gataaatgct tcaataatat
     6721 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     6781 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     6841 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     6901 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     6961 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     7021 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     7081 acagtatgat gagcacagta taagctagct tcgaagatat caagcttatc gataatcaac
     7141 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     7201 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     7261 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     7321 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     7381 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     7441 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     7501 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
     7561 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     7621 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     7681 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgacctc
     7741 gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt
     7801 gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac
     7861 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     7921 ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct
     7981 accacacaca aggctacttc cctgattggc agaactacac accagggcca gggatcagat
     8041 atccactgac ctttggatgg tgctacaagc tagtaccagt tgagcaagag aaggtagaag
     8101 aagccaatga aggagagaac acccgcttgt tacaccctgt gagcctgcat gggatggatg
     8161 acccggagag agaagtatta gagtggaggt ttgacagccg cctagcattt catcacatgg
     8221 cccgagagct gcatccggac tgtactgggt ctctctggtt agaccagatc tgagcctggg
     8281 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     8341 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     8401 tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa cccgctgatc agcctcgact
     8461 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     8521 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     8581 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     8641 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
     8701 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg
     8761 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     8821 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     8881 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     8941 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     9001 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     9061 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     9121 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt
     9181 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     9241 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     9301 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     9361 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     9421 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
     9481 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc
     9541 agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt
     9601 gaggaactaa accatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt
     9661 cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga
     9721 cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca
     9781 ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc
     9841 cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga
     9901 gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt
     9961 gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt ccaccgccgc
    10021 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
    10081 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
    10141 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
    10201 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
    10261 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
    10321 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
    10381 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
    10441 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
    10501 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
    10561 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
    10621 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
    10681 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
    10741 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
    10801 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
    10861 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
    10921 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
    10981 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
    11041 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
    11101 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
    11161 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
    11221 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
    11281 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
    11341 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
    11401 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
    11461 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
    11521 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
    11581 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
    11641 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
    11701 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
    11761 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
    11821 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
    11881 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
    11941 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
    12001 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    12061 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    12121 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    12181 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    12241 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    12301 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    12361 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    12421 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc
    12481 actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt
    12541 gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg
    12601 accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcg
//
