LOCUS       sequence-417741-        7672 bp    DNA     circular SYN 15-APR-2025
DEFINITION  mVenus-inserted PcdhgB2 lacking an intracellular domain (ICD).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7672)
  AUTHORS   Kanadome T, Hoshino N, Nagai T, Matsuda T, Yagi T
  TITLE     Development of FRET-based indicators for visualizing homophilic 
            trans interaction of a clustered protocadherin.
  JOURNAL   Sci Rep. 2021 Nov 15;11(1):22237. doi: 10.1038/s41598-021-01481-2.
  PUBMED    34782670
REFERENCE   2  (bases 1 to 7672)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2021-11-15"; volume: "11(1):22237. doi"; pages: " 
            10.1038/s41598-021-01481-2"
COMMENT     Sequence Label: pCX:gamma-B2-Delta-ICD-EC5-Venus-Delta-N3C9-P3
FEATURES             Location/Qualifiers
     source          1..7672
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        5..384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          662..1678
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1602..1624
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1686..1705
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(3281..3302)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3542..3561)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3889..3910
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4694..4713)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4759..4814
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4813..4832)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5175..5191)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5175..5191)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5188..5210)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5199..5215
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5223..5229,5230..5247,5248..5253))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5268..5289
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5348..5543
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5394..5529
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5549..5683
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5586..5605)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5640..5659
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5751..5768)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5922..6510)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6002..6021)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6681..7472,7473..7541))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7304..7323
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7542..7646)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
       61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      181 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      361 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggsgsgs gccagggggg gggggggggg gsgagggggg
      541 gggsggggsg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
      721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      781 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
      841 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
      901 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
      961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
     1021 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
     1081 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
     1141 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
     1201 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gkgggggtgc cgggcggggc
     1261 ggggccgcct cgggccgggg rgggctcggg ggrggggcgc ggcggccccc ggagcgccgg
     1321 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
     1381 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
     1441 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
     1501 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
     1561 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     1621 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     1681 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tcgccaccat
     1741 gaggaggggc tctgggagcg gtccggcctg gtggctgcag gtactgtcgc ctttcctgtt
     1801 gcttttgttc cacggagctc tcgcggatca gatccgctac tccattcccg aggagctggc
     1861 caagaactcg atagtaggaa acctcgcaag ggatctgggg ctcagtgtcc agaatctgcc
     1921 cacccggaag ctaagggtca gcgcagagaa ggattatttt actgtaaatc cagagagtgg
     1981 ggacttactt gtgggtgaca gaatagacag agagcaggtt tgcgggaagc agcctcagtg
     2041 tgttttggag ttcgatattg tcgctgagaa gcccttaaat attttccacg tagctgtaat
     2101 tcttcaggat gtgaacgaca acgctcctct attcaaacag agtgaggttg atttaaacat
     2161 tggagagtcc accagaccag gtaaagcatt tccattggat ccagccctcg atttagatgc
     2221 tggttctaat tctctgcaaa aatactatct cactgacaat gaatactttg aagtcacgga
     2281 gaaagagacc ccagatggac gtaaatatcc tgagttgatt ctaaaacgtt ttctggacag
     2341 agaagagcat aattttcacc aactggtcct cacggctgtg gatgggggag atcctccccg
     2401 aagtggcacc acccagatcc gaatccaagt cacagacatc aatgataacc cgccaatgtt
     2461 cagccaggat gtgttcagtg tcacccttcg ggaggatgta ccaccaggct tctccgtgct
     2521 tcaggtgaca gccaccgacc aggacgaggg tgtcaatgca gaaatcacct acgcttttca
     2581 caatgtggat gaacaagtgg aacggatttt taacttagac aagagaacag gagaaatcac
     2641 gacaaaggat aatttggatt tcgagacagc taagagttac accctgaatg tagaagcaaa
     2701 agatcccgga gacctagcat cccattgtag tatccaagtg aaaattctgg atgagaatga
     2761 ttgtgtaccg gaagtaattg tgacgtcagt atttaccccc ctaccagaag attctccact
     2821 aggaacagtc atcgccttga taaaaaccag agacagagac tctggagaaa atggagacgt
     2881 ctactgccat gtgttgggaa atgaagggtt tgtattgaaa tcttcttcga agaattatta
     2941 caaactagtg acagacagaa ctctagaccg ggaggcgata ccggaataca acgtcaccat
     3001 tgtagctgcc gataggggta agcccccgct ctcctctaac gtaatcatca ctctccatat
     3061 ctcagatgtc aacgacaatg caccggtttt tcaccaggcc tcctacttgg tccatgtggc
     3121 tgagaacaac ccacctggaa cctccattgc tcaagtcagt gcttctgacc cggatttggg
     3181 atccaatggc ctcatttcct actccatcat agccagcgac ctagagccaa gggcactatc
     3241 ggctagcggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     3301 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     3361 gctgaccctg aagctgatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     3421 gaccaccctg ggctacggcc tgcagtgctt cgcccgctac cccgaccaca tgaagcagca
     3481 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     3541 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     3601 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     3661 ggagtacaac tacaacagcc acaacgtcta tatcaccgcc gacaagcaga agaacggcat
     3721 caaggccaac ttcaagatcc gccacaacat cgaggacggc ggcgtgcagc tcgccgacca
     3781 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     3841 gagctaccag tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     3901 ggagttcgtg accgccgccg ggatcccacc ccctgctagc tccttcgtgt ccgtgaacca
     3961 ggacagtgga gtggtgttcg cgcagcgcgc cttcgaccac gagcagctgc gctccttcca
     4021 gctgacacta caggcgcggg accacggatc gcccacactc agtgccaatg tgagcatgcg
     4081 tgtgctggtg ggcgaccgca acgacaacgc gccacgcgtg ctgtatccca cacttgagcc
     4141 cgacggctct gcgctcttcg acatggtgcc gcgcgctgcc gagcccggat acctggtcac
     4201 caaggtggtg gctgtggatg cagactccgg acacaatgcc tggctatctt accacgtgct
     4261 gcaggccagc gatcccgggc tcttcagcct gggactgcgc acaggcgagg tgcgcacagc
     4321 gcgtgccctg ggagacaggg actcagcgcg gcagcgcctt ctggtcgctg tgcgtgatgg
     4381 tggacagcca ccgctctccg ctacagccac gctgcacctc atcttcgctg acagcctgca
     4441 ggaggtcctg ccagacctca gcgatgaccc tctaccatct gaccctcaga gtgagcttca
     4501 gttttacctg gtggtggcct tggccctgat ctcagtactc ttcctcctgt cggtgattct
     4561 ggctattgcc ctgcgtctga gacactcctc cagatcagct gcctggggct gctttcaacc
     4621 tggcctcagc tctaaccttg cgactggagt tggcgttgcc atgccaggtg ccgaagatga
     4681 tgtggtgtga agatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg
     4741 agcatctgac ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt
     4801 ttttgtgtct ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg
     4861 agtatttggt ttagagtttg gcaacatatg cccatatgct ggctgccatg aacaaaggtt
     4921 ggctataaag aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata
     4981 gaaaagcctt gacttgaggt tagatttttt ttatattttg ttttgtgtta tttttttctt
     5041 taacatccct aaaattttcc ttacatgttt tactagccag atttttcctc ctctcctgac
     5101 tactcccagt catagctgtc cctcttctct tatggagatc cctcgacctg cagcccaagc
     5161 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     5221 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     5281 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     5341 cggatccgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     5401 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     5461 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     5521 ttggaggcct aggcttttgc aaaaagctaa cttgtttatt gcagcttata atggttacaa
     5581 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     5641 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atccgctgca ttaatgaatc
     5701 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     5761 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     5821 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     5881 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     5941 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     6001 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     6061 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     6121 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     6181 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     6241 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     6301 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     6361 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     6421 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     6481 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     6541 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     6601 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     6661 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     6721 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     6781 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     6841 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     6901 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     6961 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     7021 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     7081 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     7141 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     7201 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     7261 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     7321 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     7381 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     7441 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     7501 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     7561 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     7621 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tg
//
