LOCUS       sequence-417816-        9375 bp    DNA     circular SYN 14-APR-2025
DEFINITION  pDEST vector with HygR marker for gene expression in S. cerevisiae. 
            .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9375)
  AUTHORS   Evans-Yamamoto D, Dube AK, Saha G, Plante S, Bradley D, 
            Gagnon-Arsenault I, Landry CR
  TITLE     Parallel nonfunctionalization of CK1delta/epsilon kinase ohnologs 
            following a whole-genome duplication event.
  JOURNAL   Mol Biol Evol. 2023 Nov 18:msad246. doi: 10.1093/molbev/msad246.
  PUBMED    37979156
REFERENCE   2  (bases 1 to 9375)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Evol. 2023 Nov 18:msad246. doi: 10.1093/molbev/msad246."
COMMENT     Sequence Label: pDN0604 (pDEST, HygR)
FEATURES             Location/Qualifiers
     source          1..9375
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    871..995
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1169..1271
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1272..1952
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     complement(1338..1357)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2272..2577
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2456..2475
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2618..2742)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      3007..3194
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(3062..3086)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(3346..3365)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3347..3365)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3372..3389)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3372..3388)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3381..3403)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3529..3984
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3616..3635)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3826..3847
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4123..4466
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             4467..5495
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             4467..5495
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     terminator      5503..5750
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(5519..5537)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(5772..5791)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5891..5913
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5951..5969)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    6011..6514
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        6551..6655
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6656..6724,6725..7516)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6874..6893)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7687..8275
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8176..8195
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8429..8446
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8563..8584
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8599..8604,8605..8622,8623..8629)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8637..8653
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8642..8664
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8661..8677
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8661..8677
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        8698..8716
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 gaagggaact ttacacttct cctatgcaca tatattaatg taactataac ggtcctaagg
       61 tagcgaaatt aaagtccaat gctagtagag aaggggggta acacccctcc gcgctctttt
      121 ccgatttttt tctaaaccgt ggaatatttc ggatatcctt ttgttgtttc cgggtgtaca
      181 atatggactt cctcttttct ggcaaccaaa cccatacatc gggattccta taataccttc
      241 gttggtctcc ctaacatgta ggtggcggag gggagatata caatagaaca gataccagac
      301 aagacataat gggctaaaca agactacacc aattacactg cctcattgat ggtggtacat
      361 aacgaactaa tactgtagcc ctagacttga tagccatcat catatcgaag tttcactacc
      421 ctttttccat ttgccatcta ttgaagtaat aataggcgca tgcaacttct tttctttttt
      481 tttcttttct ctctcccccg ttgttgtctc accatatccg caatgacaaa aaaatgatgg
      541 aagacactaa aggaaaaaat taacgacaaa gacagcacca acagatgtcg ttgttccaga
      601 gctgatgagg ggtatctcga agcacacgaa actttttcct tccttcattc acgcacacta
      661 ctctctaatg agcaacggta tacggccttc cttccagtta cttgaatttg aaataaaaaa
      721 aagtttgctg tcttgctatc aagtataaat agacctgcaa ttattaatct tttgtttcct
      781 cgtcattgtt ctcgttccct ttcttccttg tttctttttc tgcacaatat ttcaagctat
      841 accaagcata caaagggtgg gtcgaatcaa acaagtttgt acaaaaaagc tgaacgagaa
      901 acgtaaaatg atataaatat caatatatta aattagattt tgcataaaaa acagactaca
      961 taatactgta aaacacaaca tatccagtca ctatggcggc cgcgggtgat gctgccaact
     1021 tagcggccgc taagttggca gcatcacccg acgcactttg cgccgaataa atacctgtga
     1081 cggaagatca cttcgcagaa taaataaatc ctggtgtccc tgttgatacc gggaagccct
     1141 gggccaactt ttggcgaaaa tgagacgttg atcggcacgt aagaggttcc aactttcacc
     1201 ataatgaaat aagatcacta ccgggcgtat tttttgagtc atcgagattt tcaggagcta
     1261 aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc
     1321 atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg
     1381 ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc
     1441 cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa
     1501 tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg
     1561 agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc
     1621 tacatatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag
     1681 ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg
     1741 atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt
     1801 atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg
     1861 atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg
     1921 cggggcgtaa tctagaggat ccggcttact aaaagccaga taacagtatg cgtatttgcg
     1981 cgctgatttt tgcggtataa gaatatatac tgatatgtat acccgaagta tgtcaaaaag
     2041 aggtatgcta tgaagcagcg tattacagtg acagttgaca gcgacagcta tcagttgctc
     2101 aaggcatata tgatgtcaat atctccggtc tggtaagcac aaccatgcag aatgaagccc
     2161 gtcgtctgcg tgccgaacgc tggaaagcgg aaaatcagga agggatggct gaggtcgccc
     2221 ggtttattga aatgaacggc tcttttgctg acgagaacag gggctggtga aatgcagttt
     2281 aaggtttaca cctataaaag agagagccgt tatcgtctgt ttgtggatgt acagagtgat
     2341 attattgaca cgcccgggcg acggatggtg atccccctgg ccagtgcacg tctgctgtca
     2401 gataaagtcc cccgtgaact ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg
     2461 atgaccaccg atatggccag tgtgccggtc tccgttatcg gggaagaagt ggctgatctc
     2521 agccaccgcg aaaatgacat caaaaacgcc attaacctga tgttctgggg aatataaatg
     2581 tcaggctccc ttatacacag ccagtctgca ggtcgaccat agtgactgga tatgttgtgt
     2641 tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat
     2701 atcattttac gtttctcgtt cagctttctt gtacaaagtg gtaggaggag gaagtactag
     2761 tttaattaag agctcttaag taagtaatgg caaacagcta ttatgggtat tatgggttag
     2821 ggataacagg gtaataagct ctaagtaagt aacggccgcc accgcggtgg agctttggac
     2881 ttcttcgcca gaggtttggt caagtctcca atcaaggttg tcggcttgtc taccttgcca
     2941 gaaatttacg aaaagatgga aaagggtcaa atcgttggta gatacgttgt tgacacttct
     3001 aaataagcga atttcttatg atttatgatt tttattatta aataagttat aaaaaaaata
     3061 agtgtataca aattttaaag tgactcttag gttttaaaac gaaaattctt attcttgagt
     3121 aactctttcc tgtaggtcag gttgctttct caggtatagc atgaggtcgc tcttattgac
     3181 cacacctcta ccggcatgcc gagcaaatgc ctgcaaatcg ctccccattt cacccaattg
     3241 tagatatgct aactccagca atgagttgat gaatctcggt gtgtatttta tgtcctcaga
     3301 ggacaacacc tgttgtaatc gttcttccac acggatccca attcgcccta tagtgagtcg
     3361 tattacaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     3421 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     3481 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggac gcgccctgta
     3541 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     3601 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     3661 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     3721 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     3781 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     3841 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     3901 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     3961 acaaaatatt aacgcttaca atttcctgat gcggtatttt ctccttacgc atctgtgcgg
     4021 tatttcacac cgcgtacgct gcaggtcgac ggatccccgg gttaattaag gcgcgccaga
     4081 tctgtttagc ttgcctcgtc cccgccgggt cacccggcca gcgacatgga ggcccagaat
     4141 accctccttg acagtcttga cgtgcgcagc tcaggggcat gatgtgactg tcgcccgtac
     4201 atttagccca tacatcccca tgtataatca tttgcatcca tacattttga tggccgcacg
     4261 gcgcgaagca aaaattacgg ctcctcgctg cggacctgcg agcagggaaa cgctcccctc
     4321 acagacgcgt tgaattgtcc ccacgccgcg cccctgtaga gaaatataaa aggttaggat
     4381 ttgccactga ggttcttctt tcatatactt ccttttaaaa tcttgctagg atacagttct
     4441 cacatcacat ccgaacataa acaaccatgg gtaaaaagcc tgaactcacc gcgacgtctg
     4501 tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag ctctcggagg
     4561 gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc ctgcgggtaa
     4621 atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt gcatcggccg
     4681 cgctcccgat tccggaagtg cttgacattg gggaattcag cgagagcctg acctattgca
     4741 tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg
     4801 ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc ggccgatctt agccagacga
     4861 gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca
     4921 tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca
     4981 gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag
     5041 tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca
     5101 taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca
     5161 acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc
     5221 ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc gtatatgctc cgcattggtc
     5281 ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg
     5341 gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc
     5401 gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc
     5461 gacgccccag cactcgtccg agggcaaagg aataatctcg agtcatgtaa ttagttatgt
     5521 cacgcttaca ttcacgccct ccccccacat ccgctctaac cgaaaaggaa ggagttagac
     5581 aacctgaagt ctaggtccct atttattttt ttatagttat gttagtatta agaacgttat
     5641 ttatatttca aatttttctt ttttttctgt acagacgcgt gtacgcatgt aacattatac
     5701 tgaaaacctt gcttgagaag gttttgggac gctcgaaggc tttaacgagc tcgaattcat
     5761 cgatctctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
     5821 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     5881 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     5941 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     6001 tcttaggacg gatcgcttgc ctgtaactta cacgcgcctc gtatctttta atgatggaat
     6061 aatttgggaa tttactctgt gtttatttat ttttatgttt tgtatttgga ttttagaaag
     6121 taaataaaga aggtagaaga gttacggaat gaagaaaaaa aaataaacaa aggtttaaaa
     6181 aatttcaaca aaaagcgtac tttacatata tatttattag acaagaaaag cagattaaat
     6241 agatatacat tcgattaacg ataagtaaaa tgtaaaatca caggattttc gtgtgtggtc
     6301 ttctacacag acaagatgaa acaattcggc attaatacct gagagcagga agagcaagat
     6361 aaaaggtagt atttgttggc gatcccccta gagtctttta catcttcgga aaacaaaaac
     6421 tattttttct ttaatttctt tttttacttt ctatttttaa tttatatatt tatattaaaa
     6481 aatttaaatt ataattattt ttatagcacg tgatgaaaag gacccaggtg gcacttttcg
     6541 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     6601 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     6661 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     6721 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     6781 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     6841 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     6901 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     6961 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     7021 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     7081 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     7141 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     7201 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     7261 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     7321 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     7381 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     7441 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     7501 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     7561 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     7621 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     7681 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     7741 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     7801 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     7861 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     7921 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     7981 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     8041 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     8101 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     8161 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     8221 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     8281 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     8341 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     8401 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     8461 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     8521 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     8581 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     8641 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agctcgaaat
     8701 taaccctcac taaagggaac aaaagctggt accgggcccc ccctcgagat ccgggatcga
     8761 agaaatgatg gtaaatgaaa taggaaatca aggagcatga aggcaaaaga caaatataag
     8821 ggtcgaacga aaaataaagt gaaaagtgtt gatatgatgt atttggcttt gcggcgccga
     8881 aaaaacgagt ttacgcaatt gcacaatcat gctgactctg tggcggaccc gcgctcttgc
     8941 cggcccggcg ataacgctgg gcgtgaggct gtgcccggcg gagttttttg cgcctgcatt
     9001 ttccaaggtt taccctgcgc taaggggcga gattggagaa gcaataagaa tgccggttgg
     9061 ggttgcgatg atgacgacca cgacaactgg tgtcattatt taagttgccg aaagaacctg
     9121 agtgcatttg caacatgagt atactagaag aatgagccaa gacttgcgag acgcgagttt
     9181 gccggtggtg cgaacaatag agcgaccatg accttgaagg tgagacgcgc ataaccgcta
     9241 gagtactttg aagaggaaac agcaataggg ttgctaccag tataaataga caggtacata
     9301 caacactgga aatggttgtc tgtttgagta cgctttcaat tcatttgggt gtgcacttta
     9361 ttatgttaca atatg
//
