LOCUS       sequence-417943-        8800 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Dox inducible delivery of eGFP fused to a flotillin-1 palmitoylation
            mutant control peptide (F1A).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8800)
  AUTHORS   McClellan B, Wilson CN, Brenner AJ, Jolly CA, deGraffenried L
  TITLE     Flotillin-1 palmitoylation is essential for its stability and 
            subsequent tumor promoting capabilities.
  JOURNAL   Oncogene. 2024 Mar;43(14):1063-1074. doi: 
            10.1038/s41388-024-02946-0. Epub 2024 Feb 19.
  PUBMED    38374406
REFERENCE   2  (bases 1 to 8800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41388-024-02946-0"; journalName: "Oncogene"; date: 
            "2024-03"; volume: "43"; issue: "14"; pages: "1063-1074"
COMMENT     Sequence Label: TRE-eGFP-F1A
FEATURES             Location/Qualifiers
     source          1..8800
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..324
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    18..36
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    54..72
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    89..107
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    125..143
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    161..179
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    196..214
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    232..250
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     297..321
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      382..391
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(388..390,391..393,394..1104)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(433..454)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(694..713)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1041..1062
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        1218..1429
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             1450..1845
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             1867..1923
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(1924..2541,2542..2547,2548..2583,2584..2586,2587..2622,
                     2623..2625,2626..2661,2662..2670)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    2697..3285
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2750..2770)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3168..3179)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3384..4017
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4145..4161)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4145..4161)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4158..4180)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4169..4185
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4193..4199,4200..4217,4218..4223))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4238..4259
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4376..4393)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4547..5135)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4627..4646)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5306..6097,6098..6166))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5929..5948
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6167..6271)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    6319..6453
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6356..6375)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6410..6429
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             6621..7254
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7301..7426
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7923..8156
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8341..8385
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8648..8763
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
ORIGIN
        1 cgatatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgagtt tactccctat
       61 cagtgataga gaacgatgtc gagtttactc cctatcagtg atagagaacg tatgtcgagt
      121 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      181 cgtatgtcga gtttatccct atcagtgata gagaacgtat gtcgagttta ctccctatca
      241 gtgatagaga acgtatgtcg aggtaggcgt gtacggtggg aggcctatat aagcagagct
      301 cgtttagtga accgtcagat cgcctggaga attggctagc agaattctta gtgaacgtca
      361 gatccgctag cgctaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg
      421 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
      481 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
      541 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
      601 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
      661 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
      721 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
      781 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
      841 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
      901 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
      961 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct gagcaaagac
     1021 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     1081 ctcggcatgg acgagctgta caagtccgga ctcagatcaa tggtggctgg agggcgtgtc
     1141 tttgtcctgc ccgcaatcca acagatccag aggatctaac ttgaaaggag tgggaattgg
     1201 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
     1261 aggggtcggc aattgatccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
     1321 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
     1381 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg accggttcta
     1441 cgtacgacca tggccaagcc tttgtctcaa gaagaatcca ccctcattga aagagcaacg
     1501 gctacaatca acagcatccc catctctgaa gactacagcg tcgccagcgc agctctctct
     1561 agcgacggcc gcatcttcac tggtgtcaat gtatatcatt ttactggggg accttgtgca
     1621 gaactcgtgg tgctgggcac tgctgctgct gcggcagctg gcaacctgac ttgtatcgtc
     1681 gcgatcggaa atgagaacag gggcatcttg agcccctgcg gacggtgccg acaggtgctt
     1741 ctcgatctgc atcctgggat caaagccata gtgaaggaca gtgatggaca gccgacggca
     1801 gttgggattc gtgaattgct gccctctggt tatgtgtggg agggcatgca tagccgcggc
     1861 agcggcgcta ctaacttcag cctgctgaag caggctggag acgtggagga gaaccctgga
     1921 cctatgtcta gactggacaa gagcaaagtc ataaacggag ctctggaatt actcaatggt
     1981 gtcggtatcg aaggcctgac gacaaggaaa ctcgctcaaa agctgggagt tgagcagcct
     2041 accctgtact ggcacgtgaa gaacaagcgg gccctgctcg atgccctgcc aatcgagatg
     2101 ctggacaggc atcataccca cttctgcccc ctggaaggcg agtcatggca agactttctg
     2161 cggaacaacg ccaagtcata ccgctgtgct ctcctctcac atcgcgacgg ggctaaagtg
     2221 catctcggca cccgcccaac agagaaacag tacgaaaccc tggaaaatca gctcgcgttc
     2281 ctgtgtcagc aaggcttctc cctggagaac gcactgtacg ctctgtccgc cgtgggccac
     2341 tttacactgg gctgcgtatt ggaggaacag gagcatcaag tagcaaaaga ggaaagagag
     2401 acacctacca ccgattctat gcccccactt ctgagacaag caattgagct gttcgaccgg
     2461 cagggagccg aacctgcctt ccttttcggc ctggaactaa tcatatgtgg cctggagaaa
     2521 cagctaaagt gcgaaagcgg cgggccgacc gacgcccttg acgattttga cttagacatg
     2581 ctcccagccg atgcccttga cgactttgac cttgatatgc tgcctgctga cgctcttgac
     2641 gattttgacc ttgacatgct ccccgggtaa ctaagtaagg atcgatccaa gatatcaatc
     2701 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     2761 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     2821 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     2881 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     2941 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     3001 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     3061 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct
     3121 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     3181 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     3241 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgtt tcgcctcggc
     3301 gtccggacta gaggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     3361 ctttttaaaa gaaaagaggg gactggaagg gctaattcac tcccaacgaa gacaagatat
     3421 ccttgatctg tggatctacc acacacaagg ctacttccct gattagcaga actacacacc
     3481 agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga
     3541 gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag
     3601 cctgcatggg atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct
     3661 agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg
     3721 agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac
     3781 tggggagtgg cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt
     3841 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     3901 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     3961 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     4021 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     4081 gtgagaggcc ttgacattgc tagcgtttac cgtcgacctc tagctagagc ttggcgtaat
     4141 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     4201 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     4261 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     4321 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     4381 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     4441 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     4501 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     4561 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     4621 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     4681 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     4741 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     4801 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     4861 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     4921 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     4981 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     5041 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     5101 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     5161 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     5221 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     5281 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     5341 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     5401 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     5461 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     5521 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     5581 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     5641 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     5701 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     5761 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     5821 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     5881 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     5941 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     6001 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     6061 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     6121 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     6181 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     6241 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     6301 tcgacggatc gggagatcaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     6361 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     6421 aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat
     6481 catcccaaac ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta
     6541 ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata
     6601 tgtactacaa acttagtagt tggaagggct aattcactcc caaagaagac aagatatcct
     6661 tgatctgtgg atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg
     6721 gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc
     6781 agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct
     6841 gcatgggatg gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc
     6901 atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc
     6961 ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg
     7021 ggagtggcga gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc
     7081 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     7141 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     7201 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     7261 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     7321 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     7381 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     7441 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     7501 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     7561 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     7621 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     7681 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     7741 aagtaagacc accgcacagc aagcggccgg ccgctgatct tcagacctgg aggaggagat
     7801 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     7861 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     7921 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     7981 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     8041 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     8101 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     8161 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     8221 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     8281 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     8341 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     8401 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     8461 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     8521 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     8581 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
     8641 tatcgccttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     8701 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
     8761 ttttcgggtt tattacaggg acagcagaga tccagtttat
//
