LOCUS       sequence-418008-        8650 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Express gRNA against RUNX1T1 with puro and BFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8650)
  AUTHORS   Ishikawa M, Sugino S, Masuda Y, Tarumoto Y, Seto Y, Taniyama N, 
            Wagai F, Yamauchi Y, Kojima Y, Kiryu H, Yusa K, Eiraku M, Mochizuki 
            A
  TITLE     RENGE infers gene regulatory networks using time-series single-cell 
            RNA-seq data with CRISPR perturbations.
  JOURNAL   Commun Biol. 2023 Dec 28;6(1):1290. doi: 10.1038/s42003-023-05594-4.
  PUBMED    38155269
REFERENCE   2  (bases 1 to 8650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2023-12-28"; volume: "6(1):1290. doi"; pages: " 
            10.1038/s42003-023-05594-4"
COMMENT     Sequence Label: pKLV2-U6gRNA5(RUNX1T1 g3)-PGKpuroBFP-W
FEATURES             Location/Qualifiers
     source          1..8650
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    155..280
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             327..507
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(525..723)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(550..570)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     complement(1038..1057)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1039..1057)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1067..1084)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1067..1083)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1076..1098)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1225..1680
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1312..1331)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1522..1543
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1706..1810
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1811..1879,1880..2671)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2029..2048)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2842..3430
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3331..3350
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3584..3601
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3718..3739
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3754..3759,3760..3777,3778..3784)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3792..3808
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3797..3819
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3816..3832
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3816..3832
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3851..3871
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3853..3871
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    3897..4121
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     4110..4127
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             4579..4590
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     4988..5008
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5068..5769)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             complement(5770..5823)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(5842..6438)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5922..5942)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     6419..6438
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(6478..6977)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6526..6545)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     6919..6942
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     promoter        complement(7106..7354)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(7164..7183)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(7334..7354)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     misc_feature    7429..7546
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(7844..7888)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8073..8306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 agccttctga tgtttctaac aggccaggat taactgcgaa tcgttctagc tccctgcttg
       61 cccatactat atgttttaat ttatattttt tctttccccc tggccttaac cgaatttttt
      121 cccatcgcga tctaattctc ccccgcttaa tactgacgct ctcgcaccca tctctctcct
      181 tctagcctcc gctagtcaaa atttttggcg tactcaccag tcgccgcccc tcgcctcttg
      241 ccgtgcgcgc ttcagcaagc cgagtcctgc gtcgagagag ctcctctggt ttccctttcg
      301 ctttcaagtc cctgttcggg cgccactgct agagattttc cacactgact aaaagggtct
      361 gagggatctc tagttaccag agtcacacaa cagacgggca cacactactt gaagcactca
      421 aggcaagctt tattgaggct taagcagtgg gttccctagt tagccagaga gctcccaggc
      481 tcagatctgg tctaaccaga gagacccagt acaggcaaaa cgcgctgctt atatagacct
      541 cccaccgtac acgcctaccg cccatttgcg tcaatggggc ggagttgtta cgacattttg
      601 gaaagtcccg ttgattttgg tgccaaaaca aactcccatt gacgtcaatg gggtggagac
      661 ttggaaatcc ccgtgagtca aaccgctatc cacgcccatt gatgtactgc caaaaccgca
      721 tcaccatggt aatagcgatg actaatacgt agatgtactg ccaagtagga aagtcccata
      781 aggtcatgta ctgggcataa tgccaggcgg gccatttacc gtcattgacg tcaatagggg
      841 gcgtacttgg catatgatac acttgatgta ctgccaagtg ggcagtttac cgtaaatact
      901 ccacccattg acgtcaatgg aaagtcccta ttggcgttac tatgggaaca tacgtcatta
      961 ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg cgggccattt accgtaagtt
     1021 atgtactagt caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
     1081 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     1141 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     1201 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     1261 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     1321 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     1381 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     1441 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     1501 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     1561 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     1621 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     1681 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     1741 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     1801 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     1861 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     1921 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     1981 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     2041 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     2101 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     2161 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     2221 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     2281 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     2341 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     2401 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     2461 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     2521 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     2581 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     2641 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     2701 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     2761 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     2821 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     2881 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     2941 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     3001 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     3061 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     3121 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     3181 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     3241 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     3301 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     3361 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     3421 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     3481 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     3541 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     3601 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     3661 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     3721 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgctcc cggctcgtat
     3781 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     3841 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag cttaattaac ggccgcccat
     3901 agagcccacc gcatccccag catgcctgct attgacttcc caatcctccc ccttgctgtc
     3961 ctgccccacc ccacccccca gaatagaatg acacctactc agacaatgcg atgcaatttc
     4021 ctcattttat taggaaagga cagtgggagt ggcaccttcc agggtcaagg aaggcacggg
     4081 ggaggggcaa acaacagatg gctggcaact agaaggcaca gtcgaggctg atcagcgggt
     4141 ttaaactgct agagattttc cacactgact aaaagggtct gagggatctc tagttaccag
     4201 agtcacacaa cagacgggca cacactactt gaagcactca aggcaagctt tattgaggct
     4261 taagcagtgg gttccctagt tagccagaga gctcccaggc tcagatctgg tctaaccaga
     4321 gagactctgg tctaaccaga gagacccagt acaggcaaaa agcagatctt gacttctttg
     4381 ggagtgaatt agcccttcca gtcccccctt ttcttttaaa aagtggctaa gatctacagc
     4441 tgccttgtaa gtcattggtc ttaaaggtac ctgcggggag gcggcccaaa gggagatccg
     4501 actcgtctga gggcgaaggc gtagacgcgg aagaggccgc agagccggca gcaggccgcg
     4561 ggaaggaagg tccgctggat tgagggccga agggacgtag cagaaggacg tcccgcgcag
     4621 aatccaggtg gcaacacagg cgagcagcca aggaaaggac gatgatttcc ccgacaacac
     4681 cacggaattg tcagtgccca acagccgagc ccctgtccag cagcgggcaa ggcaggcggc
     4741 gatgagttcc gccgtggcaa tagggagggg gaaagcgaaa gtcccggaaa ggagctgaca
     4801 ggtggtggca atgccccaac cagtgggggt tgcgtcagca aacacagtgc acaccacgcc
     4861 acgttgcctg acaacgggcc acaactcctc ataaagagac agcaaccagg atttatacaa
     4921 ggaggagaaa atgaaagcca tacgggaagc aatagcatga tacaaaggca ttaaagcagc
     4981 gtatccacat agcgtaaaag gagcaacata gttaagaata ccagtcaatc tttcacaaat
     5041 tttgtaatcc agaggttgag cggccgctca attaagcttg tgccccagtt tgctagggag
     5101 gtcgcagtat ctggccactg ccacctcgtg ctgctcgacg taggtctcgt tgttggcctc
     5161 cttgattctt tccagtctgt agtccacata gtagacgcca ggcatcttga ggttcttagc
     5221 gggtttcttg gatctatatg tggtcttgat gtttgcgatc agatggctcc cgcccacgag
     5281 cttcagggcc atgtcgtttc tgccttccag gccgccgtca gcggggtaca gcgtctcggt
     5341 gaaggcctcc cagccgagtg ttttcttctg catcacaggg ccgttggatg tgaagttcac
     5401 ccctctgatc ttgacgttgt agatgaggca gccgtcctgg aggctggtgt cctgggtagc
     5461 ggtcagcacg cccccgtcct cgtatgtggt gactctctcc catgtgaagc cctcagggaa
     5521 ggactgcttg aagaagtcgg ggatgccctg ggtgtggttg atgaaggtct tgctgccgta
     5581 gaggaagcta gtagccagga tgtcgaaggc gaaggggaga gggccgccct cgaccacctt
     5641 gattctcatg gtctgggtgc cctcgtaggg cttgccttcg ccctcggatg tgcacttgaa
     5701 gtgatggttg tccacggtgc cctccatgta cagcttcatg tgcatgttct ccttaatcag
     5761 ctcgctcatt gggccaggat tctcctccac gtcaccgcat gttaggagac ttcctctgcc
     5821 ctctcctccg gacccgccgc cggcaccggg cttgcgggtc atgcaccagg tgcgcggtcc
     5881 ttcgggcacc tcgacgtcgg cggtgacggt gaagccgagc cgctcgtaga aggggaggtt
     5941 gcggggcgcg gaggtctcca ggaaggcggg caccccggcg cgctcggccg cctccactcc
     6001 ggggagcacg acggcgctgc ccagaccctt gccctggtgg tcgggcgaga cgccgacggt
     6061 ggccaggaac cacgcgggct ccttgggccg gtgcggcgcc aggaggcctt ccatctgttg
     6121 ctgcgcggcc agccgggaac cgctcaactc ggccatgcgc gggccgatct cggcgaacac
     6181 cgcccccgct tcgacgctct ccggcgtggt ccagaccgcc accgcggcgc cgtcgtccgc
     6241 gacccacacc ttgccgatgt cgagcccgac gcgcgtgagg aagagttctt gcagctcggt
     6301 gacccgctcg atgtggcggt ccggatcgac ggtgtggcgc gtggcggggt agtcggcgaa
     6361 cgcggcggcg agggtgcgta cggccctggg gacgtcgtcg cgggtggcga ggcgcaccgt
     6421 gggcttgtac tcggtcatgg taagcttcag ctgctcgaga tctagatgga tgcaggtcga
     6481 aaggcccgga gatgaggaag aggagaacag cgcggcagac gtgcgctttt gaagcgtgca
     6541 gaatgccggg cctccggagg accttcgggc gcccgccccg cccctgagcc cgcccctgag
     6601 cccgcccccg gacccacccc ttcccagcct ctgagcccag aaagcgaagg agcaaagctg
     6661 ctattggccg ctgccccaaa ggcctacccg cttccattgc tcagcggtgc tgtccatctg
     6721 cacgagacta gtgagacgtg ctacttccat ttgtcacgtc ctgcacgacg cgagctgcgg
     6781 ggcggggggg aacttcctga ctaggggagg agtagaaggt ggcgcgaagg ggccaccaaa
     6841 gaacggagcc ggttggcgcc taccggtgga tgtggaatgt gtgcgaggcc agaggccact
     6901 tgtgtagcgc caagtgccca gcggggctgc taaagcgcat gctccagact gccttgggaa
     6961 aagcgcctcc cctacccggt agaattggat ccaaaaaaag caccgactcg gtgccacttt
     7021 ttcaagttga taacggacta gccttattta aacttgctat gctgtttcca gcatagctct
     7081 taaacagaac gagggtgcga actccggtgt ttcgtccttt ccacaagata tataaagcca
     7141 agaaatcgaa atactttcaa gttacggtaa gcatatgata gtccatttta aaacataatt
     7201 ttaaaactgc aaactaccca agaaattatt actttctacg tcacgtattt tgtactaata
     7261 tctttgtgtt tacagtcaaa ttaattctaa ttatctctct aacagccttg tatcgtatat
     7321 gcaaatatga aggaatcatg ggaaataggc cctcttcctg cccgaccttg gacgcgtagg
     7381 gcccggtact aaccaaactg gatctctgct gtccctgtaa taaacccgaa aattttgaat
     7441 ttttgtaatt tgtttttgta attctttagt ttgtatgtct gttgctatta tgtctactat
     7501 tctttcccct gcactgtacc ccccaatccc cccttttctt ttaaaattgt ggatgaatac
     7561 tgccatttgt ctgcagaatt ggcgcacgca gtgccgatcc gttcactaat cgaatggatc
     7621 tgtctctgtc tctctctcca ccttcttctt ctattccttc gggcctgtcg ggtcccctcg
     7681 gggttgggag gtgggtctga aacgataatg gtgaatatcc ctgcctaact ctattcacta
     7741 tagaaagtac agcaaaaact attcttaaac ctaccaagcc tcctactatc attatgaata
     7801 attttatata ccacagccaa tttgttatgt taaaccaatt ccacaaactt gcccatttat
     7861 ctaattccaa taattcttgt tcattctttt cttgctggtt ttgcgattct tcaattaagg
     7921 agtgtattaa gcttgtgtaa ttgttaattt ctctgtccca ctccatccag gtcgtgtgat
     7981 tccaaatctg ttccagagat ttattactcc aactagcatt ccaaggcaca gcagtggtgc
     8041 aaatgagttt tccagagcaa ccccaaatcc ccaggagctg ttgatccttt aggtatcttt
     8101 ccacagccag gattcttgcc tggagctgct tgatgcccca gactgtgagt tgcaacagat
     8161 gctgttgcgc ctcaatagcc ctcagcaaat tgttctgctg ctgcactata ccagacaata
     8221 attgtctggc ctgtaccgtc agcgtcattg acgctgcgcc catagtgctt cctgctgctc
     8281 ccaagaaccc aaggaacaaa gctcctattc ccactgctct tttttctctc tgcaccactc
     8341 ttctctttgc cttggtgggt gctactccta atggttcaat ttttactact ttatatttat
     8401 ataattcact tctccaattg tccctcatat ctcctcctcc aggtctgaag atcagcgcgg
     8461 ccggccgctt gctgtgcggt ggtcttactt ttgttttgct cttcctctat cttgtctaaa
     8521 gcttccttgg tgtcttttat ctctatcctt tgatgcacac aatagagggt tgctactgta
     8581 ttatataatg atctaagttc ttctgatcct gtctgaaggg atggttgtag ctgtcccagt
     8641 atttgtctac
//
