LOCUS       sequence-418038-        9534 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Production and purification of catalytically active SynCas.v12.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9534)
  AUTHORS   Parks SC, Nguyen S, Nasrolahi S, Bhat C, Juncaj D, Lu D, Ramaswamy 
            R, Dhillon H, Fujiwara H, Buchman A, Akbari OS, Yamanaka N, 
            Boulanger MJ, Dillman AR
  TITLE     Parasitic nematode fatty acid- and retinol-binding proteins 
            compromise host immunity by interfering with host lipid signaling 
            pathways.
  JOURNAL   PLoS Pathog. 2021 Oct 29;17(10):e1010027. doi: 
            10.1371/journal.ppat.1010027. eCollection 2021 Oct.
  PUBMED    34714893
REFERENCE   2  (bases 1 to 9534)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2021-10-29"; volume: "17(10):e1010027. doi"; pages:
            " 10.1371/journal.ppat.1010027. eCollection 2021 Oct"
COMMENT     Sequence Label: T7-RBS-6xHis-SUMO-SynCas.v12 bacteria
FEATURES             Location/Qualifiers
     source          1..9534
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(16..1098)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     1060..1079
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(1099..1176)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     1382..1401
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     1485..1504
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1485..1503
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1504..1528
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1543..1565
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1580..1624
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             1631..1648
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1658..1675
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             1679..1972
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPL
                     RRLMEAFAKRQGKEMDSLRFLYDGISIQADQTPEDLDMEDNDIIEAHREQIGG"
     CDS             5882..5899
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5952..5970)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5966..6013
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      6050..6505
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6137..6156)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6347..6368
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(6598..7413)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(6598..7413)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     7321..7340
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     7405..7424
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      7535..8123
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8024..8043
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8277..8294
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8309..8448
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8374..8393)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8534..8556
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8550..8741)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
       61 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcca gggtggtttt
      121 tcttttcacc agtgagacgg gcaacagctg attgcccttc accgcctggc cctgagagag
      181 ttgcagcaag cggtccacgc tggtttgccc cagcaggcga aaatcctgtt tgatggtggt
      241 taacggcggg atataacatg agctgtcttc ggtatcgtcg tatcccacta ccgagatatc
      301 cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg ccatctgatc
      361 gttggcaacc agcatcgcag tgggaacgat accctcattc agcatttgca tggtttgttg
      421 aaaaccggac atggcactcc agtcgccttc ccgttccgct atcggctgaa tttgattgcg
      481 agtgagatat ttatgccagc cagccagacg cagacgcgcc gagacagaac ttaatgggcc
      541 cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc ccagtcgcgt
      601 accgtcttca tgggagaaaa taatactgtt gatgggtgtc tggtcagaga catcaagaaa
      661 taacgccgga acattagtgc aggcagcttc cacagcaatg gcatcctggt catccagcgg
      721 atagttaatg atcagcccac tgacgcgttg cgcgagaaga ttgtgcaccg ccgctttaca
      781 ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg ctggcaccca gttgatcggc
      841 gcgagattta atcgccgcga caatttgcga cggcgcgtgc agggccagac tggaggtggc
      901 aacgccaatc agcaacgact gtttgcccgc cagttgttgt gccacgcggt tgggaatgta
      961 attcagctcc gccatcgccg cttccacttt ttcccgcgtt ttcgcagaaa cgtggctggc
     1021 ctggttcacc acgcgggaaa cggtctgata agagacaccg gcatactctg cgacatcgta
     1081 taacgttact ggtttcacat tcaccaccct gaattgactc tcttccgggc gctatcatgc
     1141 cataccgcga aaggttttgc gccattcgat ggtgtccggg atctcgacgc tctcccttat
     1201 gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg
     1261 caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca
     1321 ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat
     1381 cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca
     1441 cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaattaatac gactcactat
     1501 aggggaattg tgagcggata acaattcccc tctagaaata attttgttta actttaagaa
     1561 ggagatatac cccatgggcg gtctgaacga catcttcgag gctcagaaaa tcgaatggca
     1621 cgaaagcagc catcatcatc atcatcacag cagcggcctg gtgccgcgcg gcagccatat
     1681 gtcggactca gaagtcaatc aagaagctaa gccagaggtc aagccagaag tcaagcctga
     1741 gactcacatc aatttaaagg tgtccgatgg atcttcagag atcttcttca agatcaaaaa
     1801 gaccactcct ttaagaaggc tgatggaagc gttcgctaaa agacagggta aggaaatgga
     1861 ctccttaaga ttcttgtacg acggtattag cattcaagct gatcagaccc ctgaagattt
     1921 ggacatggag gataacgata ttattgaggc tcacagagaa cagattggtg gaagtatgac
     1981 gactactatg aagatttcaa ttgaattcct cgagccgttt cggatgacca aatggcagga
     2041 aagcactagg cgaaacaaga acaacaagga atttgttcgc ggccaagctt ttgcaagatg
     2101 gcatagaaat aagaaggata acacaaaagg gcggccttac ataactggga cattgctgcg
     2161 gtccgcagta attagaagcg cggaaaacct gctgacattg agtgatggga aaattagcga
     2221 aaagacatgt tgtcctggga aatttgatac ggaagataaa gacaggttgc tgcaattgag
     2281 gcagcggtcc acccttcgtt ggaccgataa gaacccctgt ccagataacg ctgaaactta
     2341 ctgcccgttt tgcgaacttc tcgggagatc cggtaacgat ggaaagaagg ctgagaagaa
     2401 ggattggcgc tttcgtattc attttggcaa tctttccctt ccagggaagc ccgatttcga
     2461 cgggccaaaa gctataggca gccaacgggt acttaacagg gtcgatttca aatccggtaa
     2521 ggcccatgac ttctttaagg cttacgaagt cgaccatact aggtttcccc gcttcgaagg
     2581 ggagattacc atagataata aagtcagcgc tgaagccagg aaactgctct gtgattctct
     2641 caagtttacg gatcggctgt gtggagctct gtgcgtaatc aggttcgatg aatatacacc
     2701 agcagccgat agtgggaaac aaaccgaaaa tgtccaagca gaaccgaatg ctaatctcgc
     2761 tgaaaagacc gcagagcaaa ttattagcat tctggacgat aataagaaaa ccgaatatac
     2821 ccgcctgctt gctgatgcta ttcgttcact ccgccgctct agtaaacttg tcgctggctt
     2881 gccaaaggat catgacggga aggatgacca ttacctctgg gatatcggca agaagaagaa
     2941 agacgaaaac tctgtcacta ttaggcaaat ccttacgacc tcagcagaca ccaaggaact
     3001 caagaatgcc ggaaaatgga gagaattctg cgagaagctg ggtgaagcgc tgtacctcaa
     3061 gagtaaagac atgagtggcg gcctgaaaat tactcggaga atactgggcg atgctgaatt
     3121 ccatggaaag cccgatcggc ttgaaaagag ccggtctgtg tcaatcggat ctgtgttgaa
     3181 agaaactgtg gtatgcggcg aactggtcgc taaaacgccc ttcttcttcg gagcgataga
     3241 cgaagatgca aaacaaaccg acctgcaagt actcctcact cccgataaca agtatagact
     3301 gccaagaagc gccgtgcgag gtatactccg tcgagatctt caaacctatt ttgatagccc
     3361 atgcaatgct gagttgggtg gacggccatg catgtgtaag acgtgtagga ttatgagagg
     3421 gatcacggtg atggatgcgc gcagtgagta caatgccccg ccagaaataa ggcatcgtac
     3481 ccgcattaat cccttcacag gcacggttgc ggaaggtgcc ctgtttaata tggaagtagc
     3541 ccccgagggg attgtctttc catttcaact ccggtaccgg ggctctgaag atgggctgcc
     3601 cgatgcactg aaaacggtgt tgaaatggtg ggctgagggg caggcattca tgagtggcgc
     3661 tgcctcaacc gggaagggcc gattccggat ggaaaatgct aaatatgaaa cgctggatct
     3721 gagcgacgag aatcaaagga atgactatct taagaattgg ggatggcgtg acgagaaggg
     3781 gctcgaggaa ctgaagaaac gactgaactc aggtctgcca gagcccggta attataggga
     3841 tccaaaatgg cacgagatta acgtttccat tgagatggca agccctttta ttaatggcga
     3901 cccaatccgc gcagccgtgg acaaacgtgg tacagatgtg gttaccttcg ttaagtataa
     3961 agctgaaggg gaagaggcga aacccgtatg tgcatacaag gccgaatctt ttagaggggt
     4021 gatcagaagt gccgtggcac gcattcatat ggaagatggc gtccctttga ctgagttgac
     4081 tcacagtgac tgtgaatgtc tcctgtgcca aatctttgga agtgagtatg aagccggcaa
     4141 aataaggttt gaagatctcg tattcgaaag tgacccggaa cctgtgacct tcgatcatgt
     4201 ggccatcgat agattcactg gcggtgcagc tgataagaag aaattcgatg attcccctct
     4261 gcccggtagc cctgcaagac cgctcatgtt gaaaggctcc ttctggatcc gcagggacgt
     4321 tctcgaggac gaagagtact gtaaggcact cggtaaggct cttgcagatg tgaataatgg
     4381 cctttatccc ctcggtggaa agagcgccat cggctacgga caggtcaaga gtctgggtat
     4441 aaagggagat gataagagga tttctcgcct catgaatcct gcctttgatg agacagatgt
     4501 agccgttcca gaaaagccca aaactgatgc cgaggttcgc atcgaggcag agaaagtata
     4561 ttacccacac tatttcgtcg aaccccataa gaaggtggaa cgcgaggaga aaccctgtgg
     4621 tcatcaaaag ttccacgagg ggcgactgac aggtaaaatt cggtgtaagc tcattaccaa
     4681 gacacccctc atcgtcccag atactagtaa tgacgatttc ttcagacctg cggataaaga
     4741 agctcggaag gaaaaggacg aatatcataa atcatatgct ttcttcagac ttcataaaca
     4801 aatcatgatt cccgggagcg aattgagagg aatggtgagt agtgtctacg aaactgtgac
     4861 aaattcttgc ttcaggatat ttgatgagac taaacggttg tcatggcggc gtagcgtgtc
     4921 accgtaccga actgtagacg ataggatgat cggcaaacgt atgagcgccg atctgcggcc
     4981 gtgccatgga gattgggtgg aagatggtga tctcagtgcc ttgaatgcat atcccgagaa
     5041 aagactcctc ttgcgccacc ccaaaggact ctgccctgct tgccggctct ttggaaccgg
     5101 atcttacaag ggcagagtca ggtttggatt cgcgtcactc gaaaacgatc cggagtggct
     5161 gatcccaggc aagaatcccg gcgatccgtt tcacggcggg ccggtgatgc tctcattgtt
     5221 ggaacggcct cgcccgactt ggagtatacc gggatccgac aataagttta aagtgcctgg
     5281 cagaaagttt tacgtccacc accacgcctg gaaaaccatt aaggacggga accatcccac
     5341 aacaggcaaa gctattgaac aaagccctaa taaccgcact gtagaagctc tcgccggcgg
     5401 gaattccttt agcttcgaaa ttgcctttga gaacctgaaa gaatgggagc tgggtttgct
     5461 catccacagc ctgcaactcg aaaagggtct ggcgcataaa cttggaatgg caaagtctat
     5521 gggatttggt tcagttgaaa ttgacgtcga atcagtgcgc ctgagaaaag attggaagca
     5581 atggcggaat ggcaattccg aaattcccaa ctggttggga aaaggatttg ctaaactgaa
     5641 ggaatggttc cgggacgagc tcgattttat agaaaatctt aagaaacttc tttggtttcc
     5701 tgagggcgac caagcacccc gggtttgcta ccccatgctg cgaaagaagg acgatcctaa
     5761 tgggaatagc ggttacgaag aactcaaaga cggggaattc aagaaagaag atcggcagaa
     5821 gaagctgacc acgccgtgga caccgtgggc ataagtcgac aagcttgcgg ccgcactcga
     5881 gcaccaccac caccaccact gagatccggc tgctaacaaa gcccgaaagg aagctgagtt
     5941 ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt
     6001 gaggggtttt ttgctgaaag gaggaactat atccggattg gcgaatggga cgcgccctgt
     6061 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     6121 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     6181 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     6241 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     6301 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     6361 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     6421 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     6481 aacaaactag taacgtttac aatttcaggt ggcacttttc ggggaaatgt gcgcggaacc
     6541 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgaa ttaattctta
     6601 gaaaaactca tcgagcatca aatgaaactg caatttattc atatcaggat tatcaatacc
     6661 atatttttga aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc agttccatag
     6721 gatggcaaga tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa tacaacctat
     6781 taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag tgacgactga
     6841 atccggtgag aatggcaaaa gtttatgcat ttctttccag acttgttcaa caggccagcc
     6901 attacgctcg tcatcaaaat cactcgcatc aaccaaaccg ttattcattc gtgattgcgc
     6961 ctgagcgaga cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg
     7021 caaccggcgc aggaacactg ccagcgcatc aacaatgttt tcacctgaat caggatattc
     7081 ttctaatacc tggaatgctg ttttcccggg gatcgcagtg gtgagtaacc atgcatcatc
     7141 aggagtacgg ataaaatgct tgatggtcgg aagaggcata aattccgtca gccagtttag
     7201 tctgaccatc tcatctgtaa catcattggc aacgctacct ttgccatgtt tcagaaacaa
     7261 ctctggcgca tcgggcttcc catacaatcg atagattgtc gcacctgatt gcccgacatt
     7321 atcgcgagcc catttatacc catataaatc agcatccatg ttggaattta atcgcggcct
     7381 agagcaagac gtttcccgtt gaatatggct cataacaccc cttgtattac tgtttatgta
     7441 agcagacagt tttattgttc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     7501 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     7561 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     7621 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     7681 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     7741 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     7801 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     7861 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     7921 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     7981 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     8041 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     8101 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     8161 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     8221 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     8281 agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt
     8341 gcggtatttc acaccgcaat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     8401 aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg
     8461 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     8521 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     8581 gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc
     8641 tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata
     8701 aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg
     8761 ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg
     8821 gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta
     8881 tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca
     8941 gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg
     9001 gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt
     9061 catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt
     9121 atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac
     9181 gacaggagca cgatcatgcg cacccgtggg gccgccatgc cggcgataat ggcctgcttc
     9241 tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt
     9301 ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg
     9361 aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc
     9421 ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag
     9481 gctctcaagg gcatcggtcg agatcccggt gcctaatgag tgagctaact taca
//
