LOCUS       sequence-418109-       11491 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Lentiviral vector plasmid expressing Emerald under the spleen 
            focus-forming virus (SFFV) promoter and mCherry under the Ubiquitin 
            C (UbC) promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11491)
  TITLE     Plasmid DNA with two promoters
REFERENCE   2  (bases 1 to 11491)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLV-SFFV-Emerald-WPRE-UbC-mCherry
FEATURES             Location/Qualifiers
     source          1..11491
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    352..477
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    974..1207
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1392..1436
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1721..1838
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1991..2398
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      2413..2422
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2419..2421,2422..2424,2425..3138)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2464..2485)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2725..2744)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3072..3093
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(3252..3272)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3670..3681)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     4720..4739
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      4913..4922
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4919..5629
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5064..5082)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5332..5351
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     LTR             5724..5957
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(5982..6000)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5983..6000)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6290..6309)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6523..6542)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6642..6664
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6702..6720)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6788..6892
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6893..6961,6962..7753)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7111..7130)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7924..8512
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8413..8432
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8666..8683
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8753..8773)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8758..9087
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8938..9073
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10221..10286
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10416..10436
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10861..10995
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10898..10917)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10952..10971
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(11163..11491,1..305)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg
       61 ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg
      121 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
      181 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
      241 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
      301 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
      361 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
      421 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
      481 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
      541 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
      601 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
      661 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
      721 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
      781 gagcaaaaca aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg
      841 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
      901 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
      961 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     1021 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     1081 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     1141 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     1201 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     1261 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     1321 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     1381 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     1441 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     1501 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     1561 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     1621 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     1681 gatctcgacg gtatcgccaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     1741 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     1801 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     1861 gagatccagt ttggatcgat aagcttgata tcgaattcct gcagccccga taaaataaaa
     1921 gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa
     1981 gctagctgca gtaacgccat tttgcaaggc atggaaaaat accaaaccaa gaatagagaa
     2041 gttcagatca agggcgggta catgaaaata gctaacgttg ggccaaacag gatatctgcg
     2101 gtgagcagtt tcggccccgg cccggggcca agaacagatg gtcaccgcag tttcggcccc
     2161 ggcccgaggc caagaacaga tggtccccag atatggccca accctcagca gtttcttaag
     2221 acccatcaga tgtttccagg ctcccccaag gacctgaaat gaccctgcgc cttatttgaa
     2281 ttaaccaatc agcctgcttc tcgcttctgt tcgcgcgctt ctgcttcccg agctctataa
     2341 aagagctcac aacccctcac tcggcgcgcc agtcctccga cagactgagt cgcccggggg
     2401 gatccaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     2461 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     2521 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     2581 cccgtgccct ggcccaccct cgtgaccacc ttgacctacg gcgtgcagtg cttcgcccgc
     2641 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     2701 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     2761 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     2821 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaaggt ctatatcacc
     2881 gccgacaagc agaagaacgg catcaaggtg aacttcaaga cccgccacaa catcgaggac
     2941 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     3001 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
     3061 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     3121 gacgagctgt acaagtaaag cggccgcgac tctagagtcg acctgcaggc atgcaagctt
     3181 gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     3241 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     3301 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     3361 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     3421 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     3481 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     3541 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     3601 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     3661 tacgtccctt cggccctcaa tccagcggac cttccttccc gcgggcgccc ccctcctcac
     3721 ggcgagcgct gccacgtcag acgaagggcg cagcgagcgt cctgatcctt ccgcccggac
     3781 gctcaggaca gcggcccgct gctcataaga ctcggcctta gaaccccagt atcagcagaa
     3841 ggacatttta ggacgggact tgggtgactc tagggcactg gttttctttc cagagagcgg
     3901 aacaggcgag gaaaagtagt cccttctcgg cgattctgcg gagggatctc cgtggggcgg
     3961 tgaacgccga tgattatata aggacgcgcc gggtgtggca cagctagttc cgtcgcagcc
     4021 gggatttggg tcgcggttct tgtttgtgga tcgctgtgat cgtcacttgg tgagtagcgg
     4081 gctgctgggc tggccggggc tttcgtggcc gccgggccgc tcggtgggac ggaagcgtgt
     4141 ggagagaccg ccaagggctg tagtctgggt ccgcgagcaa ggttgccctg aactgggggt
     4201 tggggggagc gcagcaaaat ggcggctgtt cccgagtctt gaatggaaga cgcttgtgag
     4261 gcgggctgtg aggtcgttga aacaaggtgg ggggcatggt gggcggcaag aacccaaggt
     4321 cttgaggcct tcgctaatgc gggaaagctc ttattcgggt gagatgggct ggggcaccat
     4381 ctggggaccc tgacgtgaag tttgtcactg actggagaac tcggtttgtc gtctgttgcg
     4441 ggggcggcag ttatggcggt gccgttgggc agtgcacccg tacctttggg agcgcgcgcc
     4501 ctcgtcgtgt cgtgacgtca cccgttctgt tggcttataa tgcagggtgg ggccacctgc
     4561 cggtaggtgt gcggtaggct tttctccgtc gcaggacgca gggttcgggc ctagggtagg
     4621 ctctcctgaa tcgacaggcg ccggacctct ggtgagggga gggataagtg aggcgtcagt
     4681 ttctttggtc ggttttatgt acctatcttc ttaagtagct gaagctccgg ttttgaacta
     4741 tgcgctcggg gttggcgagt gtgttttgtg aagtttttta ggcacctttt gaaatgtaat
     4801 catttgggtc aatatgtaat tttcagtgtt agactagtaa attgtccgct aaattctggc
     4861 cgtttttggc ttttttgtta gacgaagctt ggtaccgagc tcggatcaat tcgccaccat
     4921 ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt
     4981 gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
     5041 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt
     5101 cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc
     5161 cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     5221 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg
     5281 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat
     5341 gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc
     5401 cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga
     5461 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa
     5521 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg
     5581 cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtaac tcgagggaat
     5641 taattcgagc tcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     5701 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     5761 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     5821 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     5881 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     5941 gtggaaaatc tctagcagca tctagaatta attccgtgta ttctatagtg tcacctaaat
     6001 cgtatgtgta tgatacataa ggttatgtat taattgtagc cgcgttctaa cgacaatatg
     6061 tacaagccta attgtgtagc atctggctta ctgaagcaga ccctatcatc tctctcgtaa
     6121 actgccgtca gagtcggttt ggttggacga accttctgag tttctggtaa cgccgtcccg
     6181 cacccggaaa tggtcagcga accaatcagc agggtcatcg ctagccagat cctctacgcc
     6241 ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc
     6301 gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc
     6361 gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcatgca
     6421 ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg
     6481 caggagtcgc ataagggaga gcgtcgaatg gtgcactctc agtacaatct gctctgatgc
     6541 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     6601 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     6661 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
     6721 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     6781 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     6841 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     6901 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     6961 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     7021 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     7081 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     7141 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     7201 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     7261 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     7321 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     7381 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     7441 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     7501 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     7561 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     7621 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     7681 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     7741 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     7801 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     7861 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     7921 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     7981 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     8041 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     8101 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     8161 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     8221 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     8281 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     8341 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     8401 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     8461 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     8521 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     8581 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     8641 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     8701 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct gtggaatgtg
     8761 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     8821 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     8881 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     8941 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     9001 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     9061 ttttttggag gcctaggctt ttgcaaaaag cttggacaca agacaggctt gcgagatatg
     9121 tttgagaata ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag acgcggactc
     9181 atgtgaaata ctggttttta gtgcgccaga tctctataat ctcgcgcaac ctattttccc
     9241 ctcgaacact ttttaagccg tagataaaca ggctgggaca cttcacatga gcgaaaaata
     9301 catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc gactgatgcc
     9361 ttctgaacaa tggaaaggca ttattgccgt aagccgtggc ggtctgtacc gggtgcgtta
     9421 ctggcgcgtg aactgggtat tcgtcatgtc gataccgttt gtatttccag ctacgatcac
     9481 gacaaccagc gcgagcttaa agtgctgaaa cgcgcagaag gcgatggcga aggcttcatc
     9541 gttattgatg acctggtgga taccggtggt actgcggttg cgattcgtga aatgtatcca
     9601 aaagcgcact ttgtcaccat cttcgcaaaa ccggctggtc gtccgctggt tgatgactat
     9661 gttgttgata tcccgcaaga tacctggatt gaacagccgt gggatatggg cgtcgtattc
     9721 gtcccgccaa tctccggtcg ctaatctttt caacgcctgg cactgccggg cgttgttctt
     9781 tttaacttca ggcgggttac aatagtttcc agtaagtatt ctggaggctg catccatgac
     9841 acaggcaaac ctgagcgaaa ccctgttcaa accccgcttt aaacatcctg aaacctcgac
     9901 gctagtccgc cgctttaatc acggcgcaca accgcctgtg cagtcggccc ttgatggtaa
     9961 aaccatccct cactggtatc gcatgattaa ccgtctgatg tggatctggc gcggcattga
    10021 cccacgcgaa atcctcgacg tccaggcacg tattgtgatg agcgatgccg aacgtaccga
    10081 cgatgattta tacgatacgg tgattggcta ccgtggcggc aactggattt atgagtgggc
    10141 cccggatctt tgtgaaggaa ccttacttct gtggtgtgac ataattggac aaactaccta
    10201 cagagattta aagctctaag gtaaatataa aatttttaag tgtataatgt gttaaactac
    10261 tgattctaat tgtttgtgta ttttagattc caacctatgg aactgatgaa tgggagcagt
    10321 ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga agaaatgcca tctagtgatg
    10381 atgaggctac tgctgactct caacattcta ctcctccaaa aaagaagaga aaggtagaag
    10441 accccaagga ctttccttca gaattgctaa gttttttgag tcatgctgtg tttagtaata
    10501 gaactcttgc ttgctttgct atttacacca caaaggaaaa agctgcactg ctatacaaga
    10561 aaattatgga aaaatattct gtaaccttta taagtaggca taacagttat aatcataaca
    10621 tactgttttt tcttactcca cacaggcata gagtgtctgc tattaataac tatgctcaaa
    10681 aattgtgtac ctttagcttt ttaatttgta aaggggttaa taaggaatat ttgatgtata
    10741 gtgccttgac tagagatcat aatcagccat accacatttg tagaggtttt acttgcttta
    10801 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
    10861 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
    10921 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
    10981 tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc
    11041 ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc
    11101 tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta
    11161 gttggaaggg ctaattcact cccaaagaag acaagatatc cttgatctgt ggatctacca
    11221 cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc
    11281 actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc
    11341 caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc
    11401 ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg
    11461 agagctgcat ccggagtact tcaagaactg c
//
