LOCUS       sequence-418260-        9406 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Mammalian Expression Plasmid of anti-Neurabin-1/Ppp1r9a (Mouse) 
            IgG2a R-mAb. Derived from hybridoma L129/93..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9406)
  AUTHORS   Mitchell KG, Gong B, Hunter SS, Burkart-Waco D, Gavira-O'Neill CE, 
            Templeton KM, Goethel ME, Bzymek M, MacNiven LM, Murray KD, Settles 
            ML, Froenicke L, Trimmer JS
  TITLE     High-volume hybridoma sequencing on the NeuroMabSeq platform enables
            efficient generation of recombinant monoclonal antibodies and scFvs 
            for neuroscience research.
  JOURNAL   Sci Rep. 2023 Sep 27;13(1):16200. doi: 10.1038/s41598-023-43233-4.
  PUBMED    37758930
REFERENCE   2  (bases 1 to 9406)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2023-09-27"; volume: "13(1):16200. doi"; pages: " 
            10.1038/s41598-023-43233-4"
COMMENT     Sequence Label: Anti-Neurabin-1/Ppp1r9a [L129/93R]
FEATURES             Location/Qualifiers
     source          1..9406
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        271..650
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        651..854
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      1398..1407
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     enhancer        2393..2696
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2697..2900
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             4157..4852
                     /codon_start=1
                     /product="CH2 and CH3 domains of the mouse IgG2a heavy 
                     chain plus the hinge region"
                     /label=Fc tag
                     /translation="PRGPTIKPCPPCKCPAPNLLGGPSVFIFPPKIKDVLMISLSPIVT
                     CVVVDVSEDDPDVQISWFVNNVEVHTAQTQTHREDYNSTLRVVSALPIQHQDWMSGKEF
                     KCKVNNKDLPAPIERTISKPKGSVRAPQVYVLPPPEEEMTKKQVTLTCMVTDFMPEDIY
                     VEWTNNGKTELNYKNTEPVLDSDGSYFMYSKLRVEKKNWVERNSYSCSVVHEGLHNHHT
                     TKSFSRTPGK"
     polyA_signal    4903..4958
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4957..4976)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7194..7212)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7280..7384
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7385..7453,7454..8245)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7603..7622)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8416..9004
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8905..8924
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9158..9175
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9292..9313
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9328..9333,9334..9351,9352..9358)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9366..9382
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9371..9393
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9390..9406
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9390..9406
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgattacg ccaagctcta gctagaggtc gaccaattct catgtttgac agcttatcat
       61 cgcagatccg ggcaacgttg ttgccattgc tgcaggcgca gaactggtag gtatggaaga
      121 tctatacatt gaatcaatat tggcaattag ccatattagt cattggttat atagcataaa
      181 tcaatattgg ctattggcca ttgcatacgt tgtatctata tcataatatg tacatttata
      241 ttggctcatg tccaatatga ccgccatgtt gacattgatt attgactagt tattaatagt
      301 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
      361 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
      421 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
      481 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt ccgcccccta
      541 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttacggg
      601 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
      661 tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
      721 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
      781 gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
      841 atataagcag agctcgttta gtgaaccgtc agatcctcac tctcttccgc atcgctgtct
      901 gcgagggcca gctgttgggc tcgcggttga ggacaaactc ttcgcggtct ttccagtact
      961 cttggatcgg aaacccgtcg gcctccgaac ggtactccgc caccgaggga cctgagcgag
     1021 tccgcatcga ccggatcgga aaacctctcg agaaaggcgt ctaaccagtc acagtcgcaa
     1081 ggtaggctga gcaccgtggc gggcggcagc gggtggcggt cggggttgtt tctggcggag
     1141 gtgctgctga tgatgtaatt aaagtaggcg gtcttgagac ggcggatggt cgaggtgagg
     1201 tgtggcaggc ttgagatcca gctgttgggg tgagtactcc ctctcaaaag cgggcattac
     1261 ttctgcgcta agattgtcag tttccaaaaa cgaggaggat ttgatattca cctggcccga
     1321 tctggccata cacttgagtg acaatgacat ccactttgcc tttctctcca caggtgtcca
     1381 ctcccaggtc caagtttgcc accatggagt ttcagaccca ggtactcatg tccctgctgc
     1441 tctgcatgtc tggtgcggcc gcagatgttt tgatgaccca aactccactc tccctgcctg
     1501 tcagtcttgg agatcaagcc tccatctcgt gtacatctag tcagagcatt gtacatcgta
     1561 ctggaaacac ctatttagaa tggtacctgc agaaaccagg ccagtctcca aagctcctga
     1621 tctacaaagt ttccaaccga ttttctgggg tcccagacag gttcagtggc agtggatcag
     1681 ggacagattt cacactcagg atcagcagag tggaggctga ggatctggga gtttattact
     1741 gctttcaagg ttcacatttt ccgctcacgt tcggtgctgg gaccacgctg gagctgaaac
     1801 gggctgatgc tgcaccaact gtatccatct tcccaccatc cagtgagcag ttaacatctg
     1861 gaggtgcctc agtcgtgtgc ttcttgaaca acttctaccc caaagacatc aatgtcaagt
     1921 ggaagattga tggcagtgaa cgacaaaatg gcgtcctgaa cagttggact gatcaggaca
     1981 gcaaagacag cacctacagc atgagcagca ccctcacgtt gaccaaggac gagtatgaac
     2041 gacataacag ctatacctgt gaggccactc acaagacatc aacttcaccc attgtcaaga
     2101 gcttcaacag gaatgagtgt tagagacaaa ggtcctgaga cgccaccacc agctccccag
     2161 ctccatccta tcttcccttc taaggtcttg gaggcttccc cacgagcgac ctaccactgt
     2221 tgcggtgctc caaacctcct ccccacctcc ttctcctcct cctccctttc cttggctttt
     2281 atcatgctaa tatttgcaga aaatattcaa taaagtgagt ctttgcactt gatagttatt
     2341 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
     2401 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
     2461 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
     2521 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtccgc
     2581 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
     2641 tacgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
     2701 tgcggttttg gcagtacacc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
     2761 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
     2821 caaaatgtcg taataacccc gccccgttga cgcaaatggg cggtaggcgt gtacggtggg
     2881 aggtctatat aagcagagct cgtttagtga accgtcagat cctcactctc ttccgcatcg
     2941 ctgtctgcga gggccagctg ttgggctcgc ggttgaggac aaactcttcg cggtctttcc
     3001 agtactctcg gatcggaaac ccgtcggcct ccgaacggta ctccgccacc gagggacctg
     3061 agcgagtccg catcgaccgg atcggaaaac ctctcgagaa aggcgtctaa ccagtcacag
     3121 tcgcaaggta ggctgagcac cgtggcgggc ggcagcgggt ggcggtcggg gttgtttctg
     3181 gcggaggtgc tgctgatgat gtaattaaag taggcggtct tgagacggcg gatggtcgag
     3241 gtgaggtgtg gcaggcttga gatccagctg ttggggtgag tactccctct caaaagcggg
     3301 cattacttct gcgctaagat tgtcagtttc caaaaacgag gaggatttga tattcaccta
     3361 gcccgatctg gccatacact tgagtgacaa tgacatccac tttgcctttc tctccacagg
     3421 tgtccactcc caggccacca tgaagctgcc tgttctgcta gtggtgctgc tattgttcac
     3481 gagtccagcc tcaagcagtg aagtgatgct ggtggagtct gggggagact tagtgaagcc
     3541 tggggggtcc ctgaaactct cctgtgcagc ctctggattc actttcagta cctatgccat
     3601 gtcttgggtt cgccagactc cggagcagag gctggagtgg gtcgcaacca ttactagtgg
     3661 tggtcgttat acctactatc cagacagtgt gaaggggcga ttcaccatct ccagagacaa
     3721 tgccaagaac accctgtacc tgcaaatgag cagtctgagg tctgaggaca cggccatgta
     3781 ttactgtgca agtccctact atggttaccc agactggtac ttcgatgtct ggggcgcagg
     3841 gaccacggtc accgtctcct cagcgcgccc aacagcccca tcggtctatc cactggcccc
     3901 tgtgtgtgga gatacaactg gctcctcggt gactctagga tgcctggtca agggttattt
     3961 ccctgagcca gtgaccttga cctggaactc tggatccctg tccagtggtg tgcacacctt
     4021 cccagctgtc ctgcagtctg acctctacac cctcagcagc tcagtgactg taacctcgag
     4081 cacctggccc agccagtcca tcacctgcaa tgtggcccac ccggcaagca gcaccaaggt
     4141 ggacaagaaa attgagccca gagggcccac aatcaagccc tgtcctccat gcaaatgccc
     4201 agcacctaac ctcttgggtg gaccatccgt cttcatcttc cctccaaaga tcaaggatgt
     4261 actcatgatc tccctgagcc ccatagtcac atgtgtggtg gtggatgtga gcgaggatga
     4321 cccagatgtc cagatcagct ggtttgtgaa caacgtggaa gtacacacag ctcagacaca
     4381 aacccataga gaggattaca acagtactct ccgggtggtc agtgccctcc ccatccagca
     4441 ccaggactgg atgagtggca aggagttcaa atgcaaggtc aacaacaaag acctcccagc
     4501 gcccatcgag agaaccatct caaaacccaa agggtcagta agagctccac aggtatatgt
     4561 cttgcctcca ccagaagaag agatgactaa gaaacaggtc actctgacct gcatggtcac
     4621 agacttcatg cctgaagaca tttacgtgga gtggaccaac aacgggaaaa cagagctaaa
     4681 ctacaagaac actgaaccag tcctggactc tgatggttct tacttcatgt acagcaagct
     4741 gagagtggaa aagaagaact gggtggaaag aaatagctac tcctgttcag tggtccacga
     4801 gggtctgcac aatcaccaca cgactaagag cttctcccgg actccgggta aatgatctag
     4861 acacgtgatt aattaaggat cccccgacct cgacctctgg ctaataaagg aaatttattt
     4921 tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg
     4981 caaatcattt ggtcgagatc cctcggagat ctctagctag aggatcgatc cccgccccgg
     5041 acgaactaaa cctgactacg acatctctgc cccttcttcg cggggcagtg catgtaatcc
     5101 cttcagttgg ttggtacaac ttgccaactg ggccctgttc cacatgtgac acgggggggg
     5161 accaaacaca aaggggttct ctgactgtag ttgacatcct tataaatgga tgtgcacatt
     5221 tgccaacact gagtggcttt catcctggag cagactttgc agtctgtgga ctgcaacaca
     5281 acattgcctt tatgtgtaac tcttggctga agctcttaca ccaatgctgg gggacatgta
     5341 cctcccaggg gcccaggaag actacgggag gctacaccaa cgtcaatcag aggggcctgt
     5401 gtagctaccg ataagcggac cctcaagagg gcattagcaa tagtgtttat aaggccccct
     5461 tgttaaccct aaacgggtag catatgcttc ccgggtagta gtatatacta tccagactaa
     5521 ccctaattca atagcatatg ttacccaacg ggaagcatat gctatcgaat tagggttagt
     5581 aaaagggtcc taaggaacag cgatatctcc caccccatga gctgtcacgg ttttatttac
     5641 atggggtcag gattccacga gggtagtgaa ccattttagt cacaagggca gtggctgaag
     5701 atcaaggagc gggcagtgaa ctctcctgaa tcttcgcctg cttcttcatt ctccttcgtt
     5761 tagctaatag aataactgct gagttgtgaa cagtaaggtg tatgtgaggt gctcgaaaac
     5821 aaggtttcag gtgacgcccc cagaataaaa tttggacggg gggttcagtg gtggcattgt
     5881 gctatgacac caatataacc ctcacaaacc ccttgggcaa taaatactag tgtaggaatg
     5941 aaacattctg aatatcttta acaatagaaa tccatggggt ggggacaagc cgtaaagact
     6001 ggatgtccat ctcacacgaa tttatggcta tgggcaacac ataatcctag tgcaatatga
     6061 tactggggtt attaagatgt gtcccaggca gggaccaaga caggtgaacc atgttgttac
     6121 actctatttg taacaagggg aaagagagtg gacgccgaca gcagcggact ccactggttg
     6181 tctctaacac ccccgaaaat taaacggggc tccacgccaa tggggcccat aaacaaagac
     6241 aagtggccac tctttttttt gaaattgtgg agtgggggca cgcgtcagcc cccacacgcc
     6301 gccctgcggt tttggactgt aaaataaggg tgtaataact tggctgattg taaccccgct
     6361 aaccactgcg gtcaaaccac ttgcccacaa aaccactaat ggcaccccgg ggaatacctg
     6421 cataagtagg tgggcgggcc aagatagggg cgcgattgct gcgatctgga ggacaaatta
     6481 cacacacttg cgcctgagcg ccaagcacag ggttgttggt cctcatattc acgaggtcgc
     6541 tgagagcacg gtgggctaat gttgccatgg gtagcatata ctacccaaat atctggatag
     6601 catatgctat cctaatctat atctgggtag cataggctat cctaatctat atctgggtag
     6661 catatgctat cctaatctat atctgggtag tatatgctat cctaatttat atctgggtag
     6721 cataggctat cctaatctat atctgggtag catatgctat cctaatctat atctgggtag
     6781 tatatgctat cctaatctgt atccgggtag catatgctat cctaatagag attagggtag
     6841 tatatgctat cctaatttat atctgggtag catatactac ccaaatatct ggatagcata
     6901 tgctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagcata
     6961 ggctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagtata
     7021 tgctatccta atttatatct gggtagcata ggctatccta atctatatct gggtagcata
     7081 tgctatccta atctatatct gggtagtata tgctatccta atctgtatcc gggtagcata
     7141 tgctatcctc atgataagct gtcaaacatg agaattttct tgaagacgaa agggcctcgt
     7201 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     7261 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     7321 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     7381 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     7441 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     7501 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     7561 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     7621 atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     7681 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     7741 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     7801 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     7861 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     7921 gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     7981 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     8041 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     8101 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     8161 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     8221 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     8281 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     8341 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     8401 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     8461 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     8521 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     8581 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     8641 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     8701 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     8761 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     8821 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     8881 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     8941 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     9001 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     9061 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     9121 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     9181 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     9241 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     9301 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     9361 tggaattgtg agcggataac aatttcacac aggaaacagc tatgac
//
