LOCUS       sequence-418276-       14135 bp    DNA     circular SYN 14-APR-2025
DEFINITION  episomal SKM (expressing mouse Sox2-A61V + Klf4 + Myc) for 
            high-quality integration-free naive reset; best for rodents and 
            primates (including human)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14135)
  AUTHORS   MacCarthy CM, Wu G, Malik V, Menuchin-Lasowski Y, Velychko T, Keshet
            G, Fan R, Bedzhov I, Church GM, Jauch R, Cojocaru V, Scholer HR, 
            Velychko S
  TITLE     Highly cooperative chimeric super-SOX induces naive pluripotency 
            across species.
  JOURNAL   Cell Stem Cell. 2024 Jan 4;31(1):127-147.e9. doi: 
            10.1016/j.stem.2023.11.010. Epub 2023 Dec 22.
  PUBMED    38141611
REFERENCE   2  (bases 1 to 14135)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2023.11.010"; journalName: "Cell Stem Cell"; date: 
            "2024-01-4- 4"; volume: "31"; issue: "1"; pages: "127-147.e9"
COMMENT     Sequence Label: pCXLE-Sox2AV-T2A-Klf4-E2A-Myc
FEATURES             Location/Qualifiers
     source          1..14135
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    26..614
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(79..99)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(497..508)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(705..724)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    770..825
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(824..843)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(1186..1202)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1186..1202)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1199..1221)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1210..1226
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1234..1240,1241..1258,1259..1264))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1279..1300
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    1389..1422
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             1883..3808
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     protein_bind    3976..4009
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      4196..5985
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(6526..6543)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6697..7285)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6777..6796)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7456..8247,8248..8316))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8079..8098
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8317..8421)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        8452..8831
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8833..9113
                     /label=chicken beta-actin promoter
     intron          9114..10130
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     10054..10076
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     10138..10157
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             11166..11219
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             12690..12749
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
ORIGIN
        1 aagcttcggg gactagtcat atgataatca acctctggat tacaaaattt gtgaaagatt
       61 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
      121 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
      181 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
      241 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
      301 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
      361 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
      421 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
      481 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
      541 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
      601 ggccgcctcc ccgcatcggt acaattcact cctcaggtgc aggctgccta tcagaaggtg
      661 gtggctggtg tggccaatgc cctggctcac aaataccact gagatctttt tccctctgcc
      721 aaaaattatg gggacatcat gaagcccctt gagcatctga cttctggcta ataaaggaaa
      781 tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat
      841 gggagggcaa atcatttaaa acatcagaat gagtatttgg tttagagttt ggcaacatat
      901 gcccatatgc tggctgccat gaacaaaggt tggctataaa gaggtcatca gtatatgaaa
      961 cagccccctg ctgtccattc cttattccat agaaaagcct tgacttgagg ttagattttt
     1021 tttatatttt gttttgtgtt atttttttct ttaacatccc taaaattttc cttacatgtt
     1081 ttactagcca gatttttcct cctctcctga ctactcccag tcatagctgt ccctcttctc
     1141 ttatggagat ccctcgacct gcagcccaag cttggcgtaa tcatggtcat agctgtttcc
     1201 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     1261 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     1321 cgctttccag tcgggaaacc tgtcgtgcca gcggatctca attccgatca tattcaataa
     1381 cccttaatat aacttcgtat aatgtatgct atacgaagtt attaggtctg aagaggagtt
     1441 tacgtccagc caagcttagg atcaattctc atgtttgaca gcttatcatc gataagctga
     1501 tcctcacagg ccgcacccag cttttcttcc gttgccccag tagcatctct gtctggtgac
     1561 cttgaagagg aagaggaggg gtcccgagaa tccccatccc taccgtccag caaaaagggg
     1621 gacgaggaat ttgaggcctg gcttgaggct caggacgcaa atcttgagga tgttcagcgg
     1681 gagttttccg ggctgcgagt aattggtgat gaggacgagg atggttcgga ggatggggaa
     1741 ttttcagacc tggatctgtc tgacagcgac catgaagggg atgagggtgg gggggctgtt
     1801 ggagggggca ggagtctgca ctccctgtat tcactgagcg tcgtctaata aagatgtcta
     1861 ttgatctctt ttagtgtgaa tcatgtctga cgaggggcca ggtacaggac ctggaaatgg
     1921 cctaggagag aagggagaca catctggacc agaaggctcc ggcggcagtg gacctcaaag
     1981 aagagggggt gataaccatg gacgaggacg gggaagagga cgaggacgag gaggcggaag
     2041 accaggagcc ccgggcggct caggatcagg gccaagacat agagatggtg tccggagacc
     2101 ccaaaaacgt ccaagttgca ttggctgcaa agggacccac ggtggaacag gagcaggagc
     2161 aggagcggga ggggcaggag caggaggggc aggagcagga ggaggggcag gagcaggagg
     2221 aggggcagga ggggcaggag gggcaggagg ggcaggagca ggaggagggg caggagcagg
     2281 aggaggggca ggaggggcag gaggggcagg agcaggagga ggggcaggag caggaggagg
     2341 ggcaggaggg gcaggagcag gaggaggggc aggaggggca ggaggggcag gagcaggagg
     2401 aggggcagga gcaggaggag gggcaggagg ggcaggagca ggaggagggg caggaggggc
     2461 aggaggggca ggagcaggag gaggggcagg agcaggaggg gcaggagggg caggaggggc
     2521 aggagcagga ggggcaggag caggaggagg ggcaggaggg gcaggagggg caggagcagg
     2581 aggggcagga gcaggagggg caggagcagg aggggcagga gcaggagggg caggaggggc
     2641 aggagcagga ggggcaggag gggcaggagc aggaggggca ggaggggcag gagcaggagg
     2701 aggggcagga ggggcaggag caggaggagg ggcaggaggg gcaggagcag gaggggcagg
     2761 aggggcagga gcaggagggg caggaggggc aggagcagga ggggcaggag gggcaggagc
     2821 aggaggaggg gcaggagcag gaggggcagg agcaggaggt ggaggccggg gtcgaggagg
     2881 cagtggaggc cggggtcgag gaggtagtgg aggccggggt cgaggaggta gtggaggccg
     2941 ccggggtaga ggacgtgaaa gagccagggg gggaagtcgt gaaagagcca gggggagagg
     3001 tcgtggacgt ggagaaaaga ggcccaggag tcccagtagt cagtcatcat catccgggtc
     3061 tccaccgcgc aggccccctc caggtagaag gccatttttc caccctgtag gggaagccga
     3121 ttattttgaa taccaccaag aaggtggccc agatggtgag cctgacgtgc ccccgggagc
     3181 gatagagcag ggccccgcag atgacccagg agaaggccca agcactggac cccggggtca
     3241 gggtgatgga ggcaggcgca aaaaaggagg gtggtttgga aagcatcgtg gtcaaggagg
     3301 ttccaacccg aaatttgaga acattgcaga aggtttaaga gctctcctgg ctaggagtca
     3361 cgtagaaagg actaccgacg aaggaacttg ggtcgccggt gtgttcgtat atggaggtag
     3421 taagacctcc ctttacaacc taaggcgagg aactgccctt gctattccac aatgtcgtct
     3481 tacaccattg agtcgtctcc cctttggaat ggcccctgga cccggcccac aacctggccc
     3541 gctaagggag tccattgtct gttatttcat ggtcttttta caaactcata tatttgctga
     3601 ggttttgaag gatgcgatta aggaccttgt tatgacaaag cccgctccta cctgcaatat
     3661 cagggtgact gtgtgcagct ttgacgatgg agtagatttg cctccctggt ttccacctat
     3721 ggtggaaggg gctgccgcgg agggtgatga cggagatgac ggagatgaag gaggtgatgg
     3781 agatgagggt gaggaagggc aggagtgatg taacttgtta ggagacgccc tcaatcgtat
     3841 taaaagccgt gtattccccc gcactaaaga ataaatcccc agtagacatc atgcgtgctg
     3901 ttggtgtatt tctggccatc tgtcttgtca ccattttcgt cctcccaaca tggggcaatt
     3961 gccggaaccc ttaatataac ttcgtataat gtatgctata cgaagttatt aggtccctcg
     4021 aagaggttca ctagcggatc tcaattgggc atacccatgt tgtcacgtca ctcagctccg
     4081 cgctcaacac cttctcgcgt tggaaaacat tagcgacatt tacctggtga gcaatcagac
     4141 atgcgacggc tttagcctgg cctccttaaa ttcacctaag aatgggagca accagcagga
     4201 aaaggacaag cagcgaaaat tcacgccccc ttgggaggtg gcggcatatg caaaggatag
     4261 cactcccact ctactactgg gtatcatatg ctgactgtat atgcatgagg atagcatatg
     4321 ctacccggat acagattagg atagcatata ctacccagat atagattagg atagcatatg
     4381 ctacccagat atagattagg atagcctatg ctacccagat ataaattagg atagcatata
     4441 ctacccagat atagattagg atagcatatg ctacccagat atagattagg atagcctatg
     4501 ctacccagat atagattagg atagcatatg ctacccagat atagattagg atagcatatg
     4561 ctatccagat atttgggtag tatatgctac ccagatataa attaggatag catatactac
     4621 cctaatctct attaggatag catatgctac ccggatacag attaggatag catatactac
     4681 ccagatatag attaggatag catatgctac ccagatatag attaggatag cctatgctac
     4741 ccagatataa attaggatag catatactac ccagatatag attaggatag catatgctac
     4801 ccagatatag attaggatag cctatgctac ccagatatag attaggatag catatgctat
     4861 ccagatattt gggtagtata tgctacccat ggcaacatta gcccaccgtg ctctcagcga
     4921 cctcgtgaat atgaggacca acaaccctgt gcttggcgct caggcgcaag tgtgtgtaat
     4981 ttgtcctcca gatcgcagca atcgcgcccc tatcttggcc cgcccaccta cttatgcagg
     5041 tattccccgg ggtgccatta gtggttttgt gggcaagtgg tttgaccgca gtggttagcg
     5101 gggttacaat cagccaagtt attacaccct tattttacag tccaaaaccg cagggcggcg
     5161 tgtgggggct gacgcgtgcc cccactccac aatttcaaaa aaaagagtgg ccacttgtct
     5221 ttgtttatgg gccccattgg cgtggagccc cgtttaattt tcgggggtgt tagagacaac
     5281 cagtggagtc cgctgctgtc ggcgtccact ctctttcccc ttgttacaaa tagagtgtaa
     5341 caacatggtt cacctgtctt ggtccctgcc tgggacacat cttaataacc ccagtatcat
     5401 attgcactag gattatgtgt tgcccatagc cataaattcg tgtgagatgg acatccagtc
     5461 tttacggctt gtccccaccc catggatttc tattgttaaa gatattcaga atgtttcatt
     5521 cctacactag tatttattgc ccaaggggtt tgtgagggtt atattggtgt catagcacaa
     5581 tgccaccact gaaccccccg tccaaatttt attctggggg cgtcacctga aaccttgttt
     5641 tcgagcacct cacatacacc ttactgttca caactcagca gttattctat tagctaaacg
     5701 aaggagaatg aagaagcagg cgaagattca ggagagttca ctgcccgctc cttgatcttc
     5761 agccactgcc cttgtgacta aaatggttca ctaccctcgt ggaatcctga ccccatgtaa
     5821 ataaaaccgt gacagctcat ggggtgggag atatcgctgt tccttaggac ccttttacta
     5881 accctaattc gatagcatat gcttcccgtt gggtaacata tgctattgaa ttagggttag
     5941 tctggatagt atatactact acccgggaag catatgctac ccgtttaggg ttaacaaggg
     6001 ggccttataa acactattgc taatgccctc ttgagggtcc gcttatcggt agctacacag
     6061 gcccctctga ttgacgttgg tgtagcctcc cgtagtcttc ctgggcccct gggaggtaca
     6121 tgtcccccag cattggtgta agagcttcag ccaagagtta cacataaagg caatgttgtg
     6181 ttgcagtcca cagactgcaa agtctgctcc aggatgaaag ccactcagtg ttggcaaatg
     6241 tgcacatcca tttataagga tgtcaactac agtcagagaa cccctttgtg tttggtcccc
     6301 ccccgtgtca catgtggaac agggcccagt tggcaagttg taccaaccaa ctgaagggat
     6361 tacatgcact gccccgcgaa gaaggggcag agatgtcgta gtcaggttta gttcgtccgg
     6421 ggcggggatc gatcctctag agtcgacgct agcggatccg ctgcattaat gaatcggcca
     6481 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     6541 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     6601 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     6661 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     6721 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     6781 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     6841 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     6901 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     6961 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     7021 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     7081 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     7141 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     7201 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     7261 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     7321 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     7381 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     7441 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     7501 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     7561 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     7621 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     7681 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     7741 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     7801 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     7861 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     7921 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     7981 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     8041 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     8101 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     8161 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     8221 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     8281 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     8341 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     8401 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgggt cgacattgat
     8461 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
     8521 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
     8581 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
     8641 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
     8701 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
     8761 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
     8821 ctattaccat ggtcgaggtg agccccacgt tctgcttcac tctccccatc tcccccccct
     8881 ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg atgggggcgg
     8941 gggggggggg gggggggcgc gcgccaggcg gggcggggcg gggcgagggg cggggcgggg
     9001 cgaggcggag aggtgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta
     9061 tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg
     9121 ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc
     9181 tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct cctccgggct
     9241 gtaattagcg cttggtttaa tgacggcttg tttcttttct gtggctgcgt gaaagccttg
     9301 aggggctccg ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg tgcgtgtgtg
     9361 tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag cgctgcgggc
     9421 gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc gggggcggtg
     9481 ccccgcggtg cggggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg
     9541 gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca accccccctg cacccccctc
     9601 cccgagttgc tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg cgtggcgcgg
     9661 ggctcgccgt gccgggcggg gggtggcggc aggtgggggt gccgggcggg gcggggccgc
     9721 ctcgggccgg ggagggctcg ggggaggggc gcggcggccc ccggagcgcc ggcggctgtc
     9781 gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac
     9841 ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc accccctcta
     9901 gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc
     9961 gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt ccgcgggggg
    10021 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
    10081 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
    10141 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attcgcggcc gccatgtata
    10201 acatgatgga gacggagctg aagccgccgg gcccgcagca agcttcgggg ggcggcggcg
    10261 gaggaggcaa cgccacggcg gcggcaaccg gcggcaacca gaagaacagc ccggaccgcg
    10321 tcaagaggcc catgaacgcc ttcatggtat ggtcccgggg gcagcggcgt aagatggccc
    10381 aggagaaccc caagatgcac aactcggaga tcagcaagcg cctgggcgcg gagtggaaac
    10441 ttttgtccga gaccgagaag cggccgttca tcgacgaggc caagcggctg cgcgtgctgc
    10501 acatgaagga gcacccggat tataaatacc ggccgcggcg gaaaaccaag acgctcatga
    10561 agaaggataa gtacacgctt cccggaggct tgctggcccc cggcgggaac agcatggcga
    10621 gcggggttgg ggtgggcgcc ggcctgggtg cgggcgtgaa ccagcgcatg gacagctacg
    10681 cgcacatgaa cggctggagc aacggcagct acagcatgat gcaggagcag ctgggctacc
    10741 cgcagcaccc gggcctcaac gctcacggcg cggcacagat gcaaccgatg caccgctacg
    10801 acgtcagcgc cctgcagtac aactccatga ccagctcgca gacctacatg aacggctcgc
    10861 ccacctacag catgtcctac tcgcagcagg gcacccccgg tatggcgctg ggctccatgg
    10921 gctctgtggt caagtccgag gccagctcca gcccccccgt ggttacctct tcctcccact
    10981 ccagggcgcc ctgccaggcc ggggacctcc gggacatgat cagcatgtac ctccccggcg
    11041 ccgaggtgcc ggagcccgct gcgcccagta gactgcacat ggcccagcac taccagagcg
    11101 gcccggtgcc cggcacggcc attaacggca cactgcccct gtcgcacatg gcatgcggct
    11161 ccggcgaggg caggggaagt cttctaacat gcggggacgt ggaggaaaat cccggcccac
    11221 tcgagatgag gcagccacct ggcgagtctg acatggctgt cagcgacgct ctgctcccgt
    11281 ccttctccac gttcgcgtcc ggcccggcgg gaagggagaa gacactgcgt ccagcaggtg
    11341 ccccgactaa ccgttggcgt gaggaactct ctcacatgaa gcgacttccc ccacttcccg
    11401 gccgccccta cgacctggcg gcgacggtgg ccacagacct ggagagtggc ggagctggtg
    11461 cagcttgcag cagtaacaac ccggccctcc tagcccggag ggagaccgag gagttcaacg
    11521 acctcctgga cctagacttt atcctttcca actcgctaac ccaccaggaa tcggtggccg
    11581 ccaccgtgac cacctcggcg tcagcttcat cctcgtcttc cccagcgagc agcggccctg
    11641 ccagcgcgcc ctccacctgc agcttcagct atccgatccg ggccgggggt gacccgggcg
    11701 tggctgccag caacacaggt ggagggctcc tctacagccg agaatctgcg ccacctccca
    11761 cggccccctt caacctggcg gacatcaatg acgtgagccc ctcgggcggc ttcgtggctg
    11821 agctcctgcg gccggagttg gacccagtat acattccgcc acagcagcct cagccgccag
    11881 gtggcgggct gatgggcaag tttgtgctga aggcgtctct gaccacccct ggcagcgagt
    11941 acagcagccc ttcggtcatc agtgttagca aaggaagccc agacggcagc caccccgtgg
    12001 tagtggcgcc ctacagcggt ggcccgccgc gcatgtgccc caagattaag caagaggcgg
    12061 tcccgtcctg cacggtcagc cggtccctag aggcccattt gagcgctgga ccccagctca
    12121 gcaacggcca ccggcccaac acacacgact tccccctggg gcggcagctc cccaccagga
    12181 ctacccctac actgagtccc gaggaactgc tgaacagcag ggactgtcac cctggcctgc
    12241 ctcttccccc aggattccat ccccatccgg ggcccaacta ccctcctttc ctgccagacc
    12301 agatgcagtc acaagtcccc tctctccatt atcaagagct catgccaccg ggttcctgcc
    12361 tgccagagga gcccaagcca aagaggggaa gaaggtcgtg gccccggaaa agaacagcca
    12421 cccacacttg tgactatgca ggctgtggca aaacctatac caagagttct catctcaagg
    12481 cacacctgcg aactcacaca ggcgagaaac cttaccactg tgactgggac ggctgtgggt
    12541 ggaaattcgc ccgctccgat gaactgacca ggcactaccg caaacacaca gggcaccggc
    12601 cctttcagtg ccagaagtgc gacagggcct tttccaggtc ggaccacctt gccttacaca
    12661 tgaagaggca ctttctcgag ggctcgggcc agtgtactaa ttatgctctc ttgaaattgg
    12721 ctggagatgt tgagagcaac ccaggtcccg gcgcgccact ggatttcctt tgggcgttgg
    12781 aaaccccgca gacagccacg acgatgcccc tcaacgtgaa cttcaccaac aggaactatg
    12841 acctcgacta cgactccgta cagccctatt tcatctgcga cgaggaagag aatttctatc
    12901 accagcaaca gcagagcgag ctgcagccgc ccgcgcccag tgaggatatc tggaagaaat
    12961 tcgagctgct tcccaccccg cccctgtccc cgagccgccg ctccgggctc tgctctccat
    13021 cctatgttgc ggtcgctacg tccttctccc caagggaaga cgatgacggc ggcggtggca
    13081 acttctccac cgccgatcag ctggagatga tgaccgagtt acttggagga gacatggtga
    13141 accagagctt catctgcgat cctgacgacg agaccttcat caagaacatc atcatccagg
    13201 actgtatgtg gagcggtttc tcagccgctg ccaagctggt ctcggagaag ctggcctcct
    13261 accaggctgc gcgcaaagac agcaccagcc tgagccccgc ccgcgggcac agcgtctgct
    13321 ccacctccag cctgtacctg caggacctca ccgccgccgc gtccgagtgc attgacccct
    13381 cagtggtctt tccctacccg ctcaacgaca gcagctcgcc caaatcctgt acctcgtccg
    13441 attccacggc cttctctcct tcctcggact cgctgctgtc ctccgagtcc tccccacggg
    13501 ccagccctga gcccctagtg ctgcatgagg agacaccgcc caccaccagc agcgactctg
    13561 aagaagagca agaagatgag gaagaaattg atgtggtgtc tgtggagaag aggcaaaccc
    13621 ctgccaagag gtcggagtcg ggctcatctc catcccgagg ccacagcaaa cctccgcaca
    13681 gcccactggt cctcaagagg tgccacgtct ccactcacca gcacaactac gccgcacccc
    13741 cctccacaag gaaggactat ccagctgcca agagggccaa gttggacagt ggcagggtcc
    13801 tgaagcagat cagcaacaac cgcaagtgct ccagccccag gtcctcagac acggaggaaa
    13861 acgacaagag gcggacacac aacgtcttgg aacgtcagag gaggaacgag ctgaagcgca
    13921 gcttttttgc cctgcgtgac cagatccctg aattggaaaa caacgaaaag gcccccaagg
    13981 tagtgatcct caaaaaagcc accgcctaca tcctgtccat tcaagcagac gagcacaagc
    14041 tcacctctga aaaggactta ttgaggaaac gacgagaaca gttgaaacac aaactcgaac
    14101 agcttcgaaa ctctggtgca taaatcgaag aattc
//
