LOCUS       sequence-419023-        9032 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Donor plasmid for knock-in tdTomato into the human SOX17 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9032)
  TITLE     Kai Wang Lab Plasmids 2023
REFERENCE   2  (bases 1 to 9032)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SOX17-tdTomato-EhB
FEATURES             Location/Qualifiers
     source          1..9032
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(9..26)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    15..239
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   430..492
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     rep_origin      complement(1388..1976)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1468..1487)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2147..2938,2939..3007))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2770..2789
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3008..3112)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3180..3198
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             3783..3800
                     /codon_start=1
                     /product="HQHQHQ metal affinity tag"
                     /label=HQ tag
                     /note="The HQ tag binds to nickel and other metal ions much
                     like the 6xHis tag, but may allow elution at lower 
                     concentrations of imidazole (Godat et al., 2008)."
                     /translation="HQHQHQ"
     CDS             3789..3806
                     /codon_start=1
                     /product="HQHQHQ metal affinity tag"
                     /label=HQ tag
                     /note="The HQ tag binds to nickel and other metal ions much
                     like the 6xHis tag, but may allow elution at lower 
                     concentrations of imidazole (Godat et al., 2008)."
                     /translation="HQHQHQ"
     CDS             4077..4133
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4134..5564
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGRYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     repeat_region   5576..5610
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    5900..5920
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        5921..7098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     primer_bind     7047..7067
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             7111..8133
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             7111..8133
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             8140..8193
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             8200..8226
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             8242..8268
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     regulatory      8290..8299
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8296..9006
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
ORIGIN
        1 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
       61 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
      121 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
      181 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggg
      241 cggccctgtc cctcataaaa gttttgttac tttatagaag aaattttgag tttttgtttt
      301 ttttaataaa taaataaaca taaataaatt gtttgttgaa tttattatta gtatgtaagt
      361 gtaaatataa taaaacttaa tatctattca aattaataaa taaacctcga tatacagacc
      421 gataaaacac atgcgtcaat tttacacatg attatcttta acgtacgtca caatatgatt
      481 atctttctag ggttaacagg tccctgatcc gccccagcct gcaggccaga agcagtgtta
      541 cacacttcct ggaggagcta aggaaatcct cagactcctg ggtttttgtt gttgctgttg
      601 ttgtttttta aaaggtgtgt tggcatataa tttatggtaa tttattttgt ctgccacttg
      661 aacagtttgg gggggtgagg tttcatttaa aatttgttca gagatttgtt tcccatagtt
      721 ggattgtcaa aaccctattt ccaagttcaa gttaactagc tttgaatgtg tcccaaaaca
      781 gcttcctcca tttcctgaaa gtttattgat caaagaaatg ttgtcctggg tgtgtttttt
      841 caatcttcta aaaaataaaa tctggaatcc tgcttttttg ctctactagt acctctgtca
      901 cactagtctt atcaaaaacc agttcttaag atcaatgtta agtttattag ttaatgtaaa
      961 tttctcatcc tcgaaaaggg tgaacataaa tgcctttaag gagtatatct aaaaataaac
     1021 attaggatat ctaagtttga tgtaattgtt tcaggaagga aaaaagaaaa gcattctgga
     1081 atgagcctac ttcaagtaat cttagtttct aaaactaaca gttaatattt tcaattccag
     1141 tatatcactt taagtagaag gggatgtcca agtaattttg gttttctaac tgttgaatca
     1201 taagcttgac ctgcccccag aggctttttg gatgttttta tctgtgtttt gccatctctt
     1261 tacactcctc gacattcagt ttaccttaat cttcacattt ttacaccttg ggaagtggca
     1321 agaattccat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     1381 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     1441 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     1501 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     1561 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     1621 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     1681 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     1741 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     1801 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     1861 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     1921 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     1981 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     2041 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     2101 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     2161 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     2221 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     2281 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     2341 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     2401 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     2461 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     2521 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     2581 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     2641 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     2701 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     2761 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     2821 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     2881 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     2941 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     3001 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     3061 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     3121 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     3181 ataggcgtat cacgaggccc tttcgtcggc gcgggtacct gcaggaccac cccaactaca
     3241 agtaccggcc gcggcggcgc aagcaggtga agcggctgaa gcgggtggag ggcggcttcc
     3301 tgcacggcct ggctgagccg caggcggccg cgctgggccc cgagggcggc cgcgtggcca
     3361 tggacggcct gggcctccag ttccccgagc agggcttccc cgccggcccg ccgctgctgc
     3421 ctccgcacat gggcggccac taccgcgact gccagagtct gggcgcgcct ccgctcgacg
     3481 gctacccgtt gcccacgccc gacacgtccc cgctggacgg cgtggacccc gacccggctt
     3541 tcttcgccgc cccgatgccc ggggactgcc cggcggccgg cacctacagc tacgcgcagg
     3601 tctcggacta cgctggcccc ccggagcctc ccgccggtcc catgcacccc cgactcggcc
     3661 cagagcccgc gggtccctcg attccgggcc tcctggcgcc acccagcgcc cttcacgtgt
     3721 actacggcgc gatgggctcg cccggggcgg gcggcgggcg cggcttccag atgcagccgc
     3781 aacaccagca ccagcaccag caccagcacc accccccggg ccccggacag ccgtcgcccc
     3841 ctccggaggc actgccctgc cgggacggca cggaccccag tcagcccgcc gagctcctcg
     3901 gggaggtgga ccgcacggaa tttgaacagt atctgcactt cgtgtgcaag cctgagatgg
     3961 gcctccccta ccaggggcat gactccggtg tgaatctccc cgacagccac ggggccattt
     4021 cctcggtggt gtccgacgca agctccgcgg tatattactg caactatcct gacgtggcga
     4081 cgaacttctc tctgttaaag caagcaggag atgttgaaga aaaccccggg cctatggtga
     4141 gcaagggcga ggaggtcatc aaagagttca tgcgcttcaa ggtgcgcatg gagggctcca
     4201 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc
     4261 agaccgccaa gctgaaggtg accaagggcg gccccctgcc cttcgcctgg gacatcctgt
     4321 ccccccagtt catgtacggc tccaaggcgt acgtgaagca ccccgccgac atccccgatt
     4381 acaagaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg
     4441 gcggtctggt gaccgtgacc caggactcct ccctgcagga cggcacgctg atctacaagg
     4501 tgaagatgcg cggcaccaac ttcccccccg acggccccgt aatgcagaag aagaccatgg
     4561 gctgggaggc ctccaccgag cgcctgtacc cccgcgacgg cgtgctgaag ggcgagatcc
     4621 accaggccct gaagctgaag gacggcggcc actacctggt ggagttcaag accatctaca
     4681 tggccaagaa gcccgtgcaa ctgcccggct actactacgt ggacaccaag ctggacatca
     4741 cctcccacaa cgaggactac accatcgtgg aacagtacga gcgctccgag ggccgccacc
     4801 acctgttcct ggggcatggc accggcagca ccggcagcgg cagctccggc accgcctcct
     4861 ccgaggacaa caacatggcc gtcatcaaag agttcatgcg cttcaaggtg cgcatggagg
     4921 gctccatgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
     4981 gcacccagac cgccaagctg aaggtgacca agggcggccc cctgcccttc gcctgggaca
     5041 tcctgtcccc ccagttcatg tacggctcca aggcgtacgt gaagcacccc gccgacatcc
     5101 ccgattacaa gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg
     5161 aggacggcgg tctggtgacc gtgacccagg actcctccct gcaggacggc acgctgatct
     5221 acaaggtgaa gatgcgcggc accaacttcc cccccgacgg ccccgtaatg cagaagaaga
     5281 ccatgggctg ggaggcctcc accgagcgcc tgtacccccg cgacggcgtg ctgaagggcg
     5341 agatccacca ggccctgaag ctgaaggacg gcggccgcta cctggtggag ttcaagacca
     5401 tctacatggc caagaagccc gtgcaactgc ccggctacta ctacgtggac accaagctgg
     5461 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgagcgc tccgagggcc
     5521 gccaccacct gttcctgtac ggcatggacg agctgtacaa gtaagtcgac cttaacccta
     5581 gaaagatagt ctgcgtaaaa ttgacgcatg cattcttgaa atattgctct ctctttctaa
     5641 atagcgcgaa tccgtcgctg tgcatttagg acatctcagt cgccgcttgg agctcccgtg
     5701 aggcgtgctt gtcaatgcgg taagtgtcac tgattttgaa ctataacgac cgcgtgagtc
     5761 aaaatgacgc atgattatct tttacgtgac ttttaagatt taactcatac gataattata
     5821 ttgttatttc atgttctact tacgtgataa cttattatat atatattttc ttgttataga
     5881 tatcagggac agccatcatc aactttgtat agaaaagttg ggctccggtg cccgtcagtg
     5941 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac
     6001 cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
     6061 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
     6121 ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc
     6181 tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct ggctgcagta
     6241 cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc
     6301 ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg
     6361 cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat
     6421 ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc
     6481 gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg
     6541 tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg
     6601 acgggggtag tctcaagctg gccggcctgc tctggtgcct ggtctcgcgc cgccgtgtat
     6661 cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg
     6721 gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg
     6781 ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg
     6841 tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga gcttttggag
     6901 tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg
     6961 ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct
     7021 ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct
     7081 tccattcagg tgtcgtgaaa gcttgccacc atgaaaaagc ctgaactcac cgcgacgtct
     7141 gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag
     7201 ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta
     7261 aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc
     7321 gcgctcccga ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc
     7381 atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct
     7441 gttctgcagc cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg
     7501 agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc
     7561 atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc
     7621 agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa
     7681 gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc
     7741 ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc
     7801 aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag
     7861 cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt
     7921 cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag
     7981 ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc
     8041 cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac
     8101 cgacgcccca gcactcgtcc gagggcaaag gaagctagcg agggcagagg aagtctgcta
     8161 acatgcggtg acgtcgagga gaatcctggc ccaccggtcc ctgccgctaa acgggtcaaa
     8221 ctcgacggcg gttctggagg tcctgctgca aagcgcgtta agttggatgg agggtcaggg
     8281 ggagccaccg ccaccatggt gtctaagggc gaagagctga ttaaggagaa catgcacatg
     8341 aagctgtaca tggagggcac cgtggacaac catcacttca agtgcacatc cgagggcgaa
     8401 ggcaagccct acgagggcac ccagaccatg agaatcaagg tggtcgaggg cggccctctc
     8461 cccttcgcct tcgacatcct ggctactagc ttcctctacg gcagcaagac cttcatcaac
     8521 cacacccagg gcatccccga cttcttcaag cagtccttcc ctgagggctt cacatgggag
     8581 agagtcacca catacgaaga cgggggcgtg ctgaccgcta cccaggacac cagcctccag
     8641 gacggctgcc tcatctacaa cgtcaagatc agaggggtga acttcacatc caacggccct
     8701 gtgatgcaga agaaaacact cggctgggag gccttcaccg agacgctgta ccccgctgac
     8761 ggcggcctgg aaggcagaaa cgacatggcc ctgaagctcg tgggcgggag ccatctgatc
     8821 gcaaacgcca agaccacata tagatccaag aaacccgcta agaacctcaa gatgcctggc
     8881 gtctactatg tggactacag actggaaaga atcaaggagg ccaacaacga gacctacgtc
     8941 gagcagcacg aggtggcagt ggccagatac tgcgacctcc ctagcaaact ggggcacaag
     9001 cttaattagt ctagagggcc cgtttaaacc cg
//
