LOCUS       sequence-419032-        8931 bp    DNA     circular SYN 14-APR-2025
DEFINITION  New color variant of Fucci (Fluorescent ubiquitination-based cell 
            cycle indicator) : Fucci(SA)5..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8931)
  AUTHORS   Ando R, Sakaue-Sawano A, Shoda K, Miyawaki A
  TITLE     Two coral fluorescent proteins of distinct colors for sharp 
            visualization of cell-cycle progression.
  JOURNAL   Cell Struct Funct. 2023 Jun 30. doi: 10.1247/csf.23028.
  PUBMED    37394513
REFERENCE   2  (bases 1 to 8931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Struct
            Funct. 2023 Jun 30. doi: 10.1247/csf.23028."
COMMENT     Sequence Label: tFucci(SA)5
FEATURES             Location/Qualifiers
     source          1..8931
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     68..90
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     82..99
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     83..99
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     109..128
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        109..127
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   157..191
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        494..873
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          1170..2186
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2110..2132
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2194..2213
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      2253..2262
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2979..3251
                     /codon_start=1
                     /product="degron consisting of residues 30-120 of human 
                     Cdt1 (Zielke and Edgar, 2015)"
                     /label=Cdt1 (30-120)
                     /translation="PSPARPALRAPASATSGSRKRARPPAAPGRDQARPPARRRLRLSV
                     DEVSSPSTPEAPDIPACPSPGQKIKKSTPAAGQPPHLTSAQDQDTI"
     CDS             3279..3335
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4077..4406
                     /codon_start=1
                     /product="degron consisting of residues 1-110 of human 
                     Geminin (Zielke and Edgar, 2015)"
                     /label=Gem1 (1-110)
                     /translation="MNPSMKQKQEEIKENIKNSSVPRRTLKMIQPSASGSLVGRENELS
                     AGLSKRKHRNDHLTSTTSSPGVIVPESSENKNLGGVTQESFDLMIKENPSSQYWKEVAE
                     KRRKAL"
     misc_feature    4478..5027
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4622..4639)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4849..4868
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(5498..5515)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5504..5728
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5775..5791)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5799..5815
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5823..5829,5830..5847,5848..5853))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5868..5889
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   6123..6185
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6226..6246)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6226..6244)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6265..6281)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6289..6305
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6313..6319,6320..6337,6338..6343))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6358..6379
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6496..6513)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6667..7255)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6747..6766)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7426..8217,8218..8286))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8049..8068
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8287..8391)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8417..8872)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8554..8575)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8766..8785
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
       61 gggttttccc agtcacgacg ttgtaaaacg acggccagtg agcgcgcgta atacgactca
      121 ctatagggcg aattggggcg cgccattcta gattaaccct agaaagatag tctgcgtaaa
      181 attgacgcat gcattcttga aatattgctc tctctttcta aatagcgcga atccgtcgct
      241 gtgcatttag gacatctcag tcgccgcttg gagctcccgt gaggcgtgct tgtcaatgcg
      301 gtaagtgtca ctgattttga actataacga ccgcgtgagt caaaatgacg catgattatc
      361 ttttacgtga cttttaagat ttaactcata cgataattat attgttattt catgttctac
      421 ttacgtgata acttattata tatatatttt cttgttatag atatcaacta gaatgctagc
      481 atgggcccat ctcgacattg attattgact agttattaat agtaatcaat tacggggtca
      541 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      601 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      661 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      721 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      781 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      841 tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc
      901 actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta
      961 ttttgtgcag cgatgggggc gggggggggg gggggggggg ccccgggggg gggggggggg
     1021 gggggggggg gggggggggg gaggggcggg gcggggcgag gcggagaggt gcggcggcag
     1081 ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc
     1141 cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gcgctgcctt cgccccgtgc
     1201 cccgctccgc cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac
     1261 aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac
     1321 ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt
     1381 gcggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg
     1441 gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc
     1501 gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg
     1561 aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg
     1621 cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg agttgctgag cacggcccgg
     1681 cttcgggtgc ggggctccgt acggggcgtg gcgcggggct cgccgtgccg ggcggggggt
     1741 ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg
     1801 aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt
     1861 gccttttatg gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa atctgtgcgg
     1921 agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg gcgcggggcg aagcggtgcg
     1981 gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc
     2041 ttctccctct ccagcctcgg ggctgtccgc ggggggacgg ctgccttcgg gggggacggg
     2101 gcagggcggg gttcggcttc tggcgtgtga ccggcggctc tagagcctct gctaaccatg
     2161 ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc
     2221 atcattttgg caaagaattg gtcgacggat ccgccaccat ggagaacgtc aggagaaaga
     2281 caggcattca gacggaaatg aaaacgaagc tccacatgga tggtatggtg aatggacaca
     2341 gctttgagat caaaggggag gggaagggaa gcccgtacga gggggtgcag acaatgaagc
     2401 tgaaggttac taagggcgcc ccacttccct tctctataga cattcttttg cctcagtgca
     2461 tgtatggcag caaacctttc attaaatatc ctgagaacat ccccgattat ttgaaactct
     2521 catttccaga gggtatcact tgggagagga caatgacatt cgaggacggc gcagtatgcg
     2581 atgtatctaa tgattccaga ctggtcggca attgctttat ctacactgtg aagtttcagg
     2641 gagtcaattt tccacttgat ggccccgtta tgcaaaagaa gacccgaggc tgggaaccct
     2701 ccactgaagt gctgtacgaa tgtgatgggt ggatgcgcgg gttggtggac atagctctga
     2761 aactggaaaa cggtggacat tatatgtgta attttaagac cacctacaaa tccaagaaag
     2821 gtcttgaggt gccaccttac catttcgtgg atcataaact cgacctgctc agtcataaca
     2881 ccgacggagc cacctttgag gagttcgaac aaggtgaaat cgctcacgcg cacttgagta
     2941 aactggccgg atatccatca cactggcggc cgctcgagcc cagccccgcc aggcccgcac
     3001 tccgcgcccc ggcctccgct accagtggca gccgcaagcg cgcccgcccg cccgccgccc
     3061 ccggacgcga ccaggccagg ccaccggccc gcaggagact gcggctgtcg gtggacgagg
     3121 tttccagccc cagtaccccc gaggccccag acatcccagc ctgcccttct ccgggccaga
     3181 agataaagaa atccaccccg gcagcaggtc agccgcccca cctgacatcc gcgcaggacc
     3241 aggacaccat caccggtcgc gccaagcgcg gcagcggcgc caccaacttc agcctgctga
     3301 agcaggccgg cgacgtggag gagaaccccg gcccccgcga attcatgagt aaactccaga
     3361 aaggtgtgga gaaagagatg aagattaagc tcactatgaa ctgcaccgtg aacgaacgga
     3421 actttgtgat cacagggcag ggggctgggg aaccttatga tggaactcag acactttacc
     3481 tgacagtgga gggcggaaag acactgaact tcagcttcga tgtactgacc ccagcctttc
     3541 agtacggcaa tagggcgttt accaagtacc ccggtaacat accagacttc ttcaaacaga
     3601 ccttcagtgg cgggggatac acatggaaaa gaacaatgac ctatgaagac ggcggcgtct
     3661 caaccgttga gtccgatatc agcgttcaag gggactgctt ccattataaa atacaattca
     3721 acggaaagtt ccccccccac ggcccagtca tgcaaaagga aacagtcaaa tgggaacctt
     3781 ccaccgaggt gatgtacaag gacgataaaa acgatggagt gctgaagggc gacgtgaata
     3841 tggccctgct gttgaaggat ggtggtcacc tcagagtcga tttcaacact tcttatattc
     3901 ccaaaaataa ggtggagaag atgccagatt accacttcgt ggaccaccgg atcgagatcc
     3961 tggagaagcc cgaaggacgc cctgtcaagc tctacgcagg ggctgtggcc aggtacagcc
     4021 tgctgcctga aaagaacctg aataagggat atccatcaca ctggcggccg ctcgagatga
     4081 atcccagtat gaagcagaaa caagaagaaa tcaaagagaa tataaagaat agttctgtcc
     4141 caagaagaac tctgaagatg attcagcctt ctgcatctgg atctcttgtt ggaagagaaa
     4201 atgagctgtc cgcaggcttg tccaaaagga aacatcggaa tgaccactta acatctacaa
     4261 cttccagccc tggggttatt gtcccagaat ctagtgaaaa taaaaatctt ggaggagtca
     4321 cccaggagtc atttgatctt atgattaaag aaaatccatc ctctcagtat tggaaggaag
     4381 tggcagaaaa acggagaaag gcgctgtaat ctagagttaa ctaacttaag ctagcaacgg
     4441 tttccctcta gcgggatcaa ttccgccccc ccccctaacg ttactggccg aagccgcttg
     4501 gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc
     4561 aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc
     4621 cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa
     4681 gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct
     4741 ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca
     4801 caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca
     4861 agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat
     4921 ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc
     4981 cccccgaacc acggggacgt ggttttcctt tgaaaaacac gataatacca tggtcatgaa
     5041 aacatttaac atttctcaac aagatctaga attagtagaa gtagcgacag agaagattac
     5101 aatgctttat gaggataata aacatcatgt gggagcggca attcgtacga aaacaggaga
     5161 aatcatttcg gcagtacata ttgaagcgta tataggacga gtaactgttt gtgcagaagc
     5221 cattgcgatt ggtagtgcag tttcgaatgg acaaaaggat tttgacacga ttgtagctgt
     5281 tagacaccct tattctgacg aagtagatag aagtattcga gtggtaagtc cttgtggtat
     5341 gtgtagggag ttgatttcag actatgcacc agattgtttt gtgttaatag aaatgaatgg
     5401 caagttagtc aaaactacga ttgaagaact cattccactc aaatataccc gaaattaaaa
     5461 gtcgagatgc atcgatgatc tagagctcgc tgatcagcct cgactgtgcc ttctagttgc
     5521 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     5581 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     5641 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     5701 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaacctg cagcccaagc
     5761 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     5821 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     5881 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     5941 cggatcttaa aagttttgtt actttataga agaaattttg agtttttgtt tttttttaat
     6001 aaataaataa acataaataa attgtttgtt gaatttatta ttagtatgta agtgtaaata
     6061 taataaaact taatatctat tcaaattaat aaataaacct cgatatacag accgataaaa
     6121 cacatgcgtc aattttacgc atgattatct ttaacgtacg tcacaatatg attatctttc
     6181 tagggttaat ctagtatacg cgtttaatta atccagcttt tgttcccttt agtgagggtt
     6241 aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     6301 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     6361 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     6421 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     6481 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     6541 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     6601 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     6661 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     6721 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     6781 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     6841 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     6901 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     6961 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     7021 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     7081 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     7141 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     7201 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     7261 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     7321 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     7381 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     7441 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     7501 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     7561 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     7621 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     7681 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     7741 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     7801 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     7861 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     7921 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     7981 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     8041 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     8101 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     8161 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     8221 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     8281 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     8341 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     8401 ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
     8461 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
     8521 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     8581 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
     8641 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     8701 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     8761 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
     8821 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg
     8881 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc g
//
