LOCUS       sequence-41996-e         860 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Stempin CC, Chi L, Giraldo-Vela JP, High AA, Hacker H, Redecke V
  TITLE     The E3 Ubiquitin Ligase Mind Bomb-2 (MIB2) Protein Controls B-cell 
            CLL/Lymphoma 10 (BCL10)-dependent NF-{kappa}B Activation.
  JOURNAL   J Biol Chem. 2011 Oct 28;286(43):37147-57. Epub 2011 Sep 6.
  PUBMED    21896478
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2011-10-28"; volume: "286(43)"; pages: "37147-57. 
            Epub 2011 Sep 6"
COMMENT     Sequence Label: p3F-Strep-hMIB2
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             20..85
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             98..121
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             158..181
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 aggattcgcc accaccatgg actacaaaga ccatgacggt gattataaag atcatgatat
       61 cgattacaag gatgacgatg acaagcttgc aagcgcgtgg agccacccgc agttcgagaa
      121 aggtggaggt tccggaggtg gatcgggagg tggatcgtgg agccacccgc agttcgaaaa
      181 aggcgcggat cgatccgagg gtaaatcctc aggatctggc tccgaatcca aagtcgacac
      241 tagtatggct aggtcactgg cgcgatgcgg gccgtcctct cggctgatgg gttggaagcc
      301 cagcgaggct agaggccagt cccaaagttt ccaggcatca gggctgcagc ccaggagcct
      361 caaggcggcc cggcgggcga ctggacggcc ggacaggtcc cgagcagccc cgcccaacat
      421 ggacccagac ccccaggcgg gcgtgcaggt gggcatgcgg gtggtgcgcg gcgtggactg
      481 gaagtggggc cagcaggacg gcggcgaggg cggcgtgggc acggtggtgg agcttggccg
      541 ccacggcagc ccctcgacac ccgaccgcac agtggtcgtg cagtgggacc agggcacgcg
      601 caccaactac cgcgccggct accagggcgc gcacgacctg ctgctgtacg acaacgccca
      661 gatcggcgtc cggcacccca acatcatctg tgactgctgc aagaagcacg ggctgcgggg
      721 gatgcgctgg aagtgccgtg tgtgcctgga ctacgacctc tgcacgcagt gctacatgca
      781 caacaagcat gagctcgccc acgccttcga ccgctacgag accgctcact cgcgccctgt
      841 cacactgagt ccccgccagg
//
