LOCUS       sequence-420440-       16389 bp    DNA     circular SYN 15-APR-2025
DEFINITION  genomic integration of genes (inserted in I-SceI-site) via 
            transposon Tn7 with GmR and PE-H.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16389)
  TITLE     HOLD-Jaeger Lab pYT plasmids
REFERENCE   2  (bases 1 to 16389)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pYTSK58K_6G7
FEATURES             Location/Qualifiers
     source          1..16389
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  1204..1369
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     promoter        join(2875..2880,2881..2897,2898..2903)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             3092..3625
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     mobile_element  9776..10000
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     misc_feature    10689..10981
                     /label=CEN4
                     /note="S. cerevisiae CEN4 centromere"
     rep_origin      11629..12082
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1"
     primer_bind     complement(12110..12132)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    12218..12358
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     12273..12292
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(12373..12390)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12544..13132)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12624..12643)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(13311..14105)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(13422..13441)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     14032..14051
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        14543..14758
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             14759..15562
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
ORIGIN
        1 ctagtcccgg gtccacatat ccacgggctg gcaagggagc gcagcgaccg cgcagggcga
       61 agcccggaga gcaagcccgt agggcgccgc agccgccgta ggcggtcacg actttgcgaa
      121 gcaaagtcta gtgagtatac tcaagcattg agtggcccgc cggaggcacc gccttgcgct
      181 gcccccgtcg agccggttgg acaccaaaag ggaggggcag gcatggcggc atacgcgatc
      241 atgcgatgca agaagctggc gaaaatgggc aacgtggcgg ccagtctcaa gcacgcctac
      301 cgcgagcgcg agacgcccaa cgctgacgcc agcaggacgc cagagaacga gcactgggcg
      361 gccagcagca ccgatgaagc gatgggccga ctgcgcgagt tgctgccaga gaagcggcgc
      421 aaggacgctg tgttggcggt cgagtacgtc atgacggcca gcccggaatg gtggaagtcg
      481 gccagccaag aacagcaggc ggcgttcttc gagaaggcgc acaagtggct ggcggacaag
      541 tacggggcgg atcgcatcgt gacggccagc atccaccgtg acgaaaccag cccgcacatg
      601 accgcgttcg tggtgccgct gacgcaggac ggcaggctgt cggccaagga gttcatcggc
      661 aacaaagcgc agatgacccg cgaccagacc acgtttgcgg ccgctgtggc cgatctaggg
      721 ctgcaacggg gcatcgaggg cagcaaggca cgtcacacgc gcattcaggc gttctacgag
      781 gccctggagc ggccaccagt gggccacgtc accatcagcc cgcaagcggt cgagccacgc
      841 gcctatgcac cgcagggatt ggccgaaaag ctgggaatct caaagcgcgt tgagacgccg
      901 gaagccgtgg ccgaccggct gacaaaagcg gttcggcagg ggtatgagcc tgccctacag
      961 gccgccgcag gagcgcgtga gatgcgcaag aaggccgatc aagcccaaga gacggcccga
     1021 gaccttcggg agcgcctgaa gcccgttctg gacgccctgg ggccgttgaa tcgggatatg
     1081 caggccaagg ccgccgcgat catcaaggcc gtgggcgaaa agctgctgac ggaacagcgg
     1141 gaagtccagc gccagaaaca ggcccagcgc cagcaggaac gcgggcgcgc acatttcccc
     1201 gaatgtgggc ggacaaaata gttgggaact gggaggggtg gaaatggagt ttttaaggat
     1261 tatttaggga agagtgacaa aatagatggg aactgggtgt agcgtcgtaa gctaatacga
     1321 aaattaaaaa tgacaaaata gtttggaact agatttcact tatctggttt atctgcagca
     1381 ctcaacagat ctccgtctac ttgcgacacc tctcttaagg tagctggacc ggattcgggt
     1441 ctagcctata tcgtcaatcc gttgatctat gtcgggtgcg gagaaagagg taatgaaatg
     1501 ggcgatcgcc cacagcttac acagacctaa tcgtagagtt cctagtaata cttagtggct
     1561 cgttacctcg ttcttccagg ccagggggcc gtacgcgctc ggcgaacagt ataagcagat
     1621 tcgactcact agggataaca gggtaatctc tgggtatcga ctcaatctcg cgctattgtg
     1681 ctatatccgt ggacggttgc ggtcaattct gtcggaaaat caacggatac gaacaattac
     1741 tcgcgacttc ctcaatacgt gctgcaaaac tttagaagga gatataccat gccatttaac
     1801 aagaaaagcg ttcttgccct ctgtggcgct ggcgcgctgc tgttctcgat gtctgccctg
     1861 gccaacaacc cggcaccgac cgatccgggt gattcgggcg gtggttccgc ctatcagcgc
     1921 ggtccggatc cgtccgtcag cttcctcgaa gccgatcgcg gtcagtacag cgtccgttcc
     1981 agccgtgtct ccagcctggt cagcggcttt ggcggcggca ccatttacta tccgacaggc
     2041 acaaccggca ctatgggtgc ggtcgttgta attcccggct tcgtttctgc cgagtcttcg
     2101 attgactggt ggggcccgaa actggcgtcc tatggtttcg tggtcatgac cattgacacc
     2161 aacaccggct ttgatcagcc gccgagccgt gcgcgtcaga tcaacaacgc actggattat
     2221 ctggttagcc agaacagccg cagctccagc ccggttcgcg gcatgatcga caccaatcgt
     2281 ctgggcgtca ttggctggtc gatgggcggt ggcggcaccc tgcgtgttgc cagtgaaggt
     2341 cgtatcaagg cagccattcc gctggcgccc tgggatacaa ccagctacta cgccagccgc
     2401 tcgcaggcac cgactctgat ctttgcctgt gagtcggatg tgatcgcgcc ggtcctccag
     2461 catgcctcac cgttctacaa ttccctgcct tccagcatcg acaaggcctt tgtggaaatc
     2521 aatggtggca gccactactg cggtaatggc ggcagcatct acaacgatgt gctgagccgg
     2581 ttcggggtgt cctggatgaa actgcatctg gatgaagaca gccgctacaa gcagttcctc
     2641 tgcggaccga accacacttc cgactctcag atctccgatt atcgcggcaa ctgcccgtac
     2701 taacgcgcgc gtaactataa cggtcctaag gtagcgagct tacttgtgct gttgtgtaag
     2761 gacacgctta acccagagaa ttccagcgac ctattctacg actacgcgtc catggctcga
     2821 ggagctcggt accgccccac tacccgattt atcaagattt ggcggagagt cccattgaca
     2881 taagcctgtt cggttcgtaa actgtaatgc aagtagcgta tgcgctcacg caactggtcc
     2941 agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat
     3001 gactgttttt ttgtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     3061 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagcaacg atgttacgca
     3121 gcagggcagt cgccctaaaa caaagttagg tggctcaagt atgggcatca ttcgcacatg
     3181 taggctcggc cctgaccaag tcaaatccat gcgggctgct cttgatcttt tcggtcgtga
     3241 gttcggagac gtagccacct actcccaaca tcagccggac tccgattacc tcgggaactt
     3301 gctccgtagt aagacattca tcgcgcttgc tgccttcgac caagaagcgg ttgttggcgc
     3361 tctcgcggct tacgttctgc ccaagtttga gcagccgcgt agtgagatct atatctatga
     3421 tctcgcagtc tccggcgagc accggaggca gggcattgcc accgcgctca tcaatctcct
     3481 caagcatgag gccaacgcgc ttggtgctta tgtgatctac gtgcaagcag attacggtga
     3541 cgatcccgca gtggctctct atacaaagtt gggcatacgg gaagaagtga tgcactttga
     3601 tatcgaccca agtaccgcca cctaagtcga ctggcaaaca gctattatgg gtattatggg
     3661 tgtgtatcca gaacgacgga tcttgacgca atttggcgtg gctctcttaa ggtagccctc
     3721 cgaccaattg aggtgcgctc tgaccgatca gtttagtcac tcttccccat aaaccatttc
     3781 caagaatctt tgtgcttcct cagttatccg ctctttagat aacgttgcac ttcttaataa
     3841 tcgccagcgc ctaagctcaa catcttcctg tttaagctta ataaaagctt ggctaatccg
     3901 tctaatttga taatcttcca cactctctga gtaacgcttt aagcaaagcg ctaccaaagg
     3961 cagtttctgt agattttttg caagagaggt tccgttagga gtttgcttta acagccagct
     4021 cgatgtcgct cttgggtgat caaggctact atctagacgc tttatgattc ttaatagttg
     4081 tcgtacagca attcgatctc tttggttcca atcaactcta ggggcgggtg ccagacgctc
     4141 ttgttgatgc tgttgattcc agtgaactag ccaatccctg tcatgtcggt aaagccaagc
     4201 gtatagcacc ccaccctcta aagactgtct tgccgcctta attccttggt atttatgcac
     4261 tagttgctgc cagtctttat gcttaacaga taaatcttca gagttaggtt gcacagactg
     4321 gctaacaggt cttgtcgtta tagagtgctc agtaagagca cttgcctgct gtagcgcttc
     4381 tataaccgtt agttttggca ataaggcttg ccacacaata ctatgctgta aataactaaa
     4441 ggcttttcta tgcttacgga atatactttt cagccaacac gtgtccttgt tctctgccaa
     4501 ctttaaatcc agtttctcta gtgcctcatc actaaaagtc tgcctcactc tctccgccac
     4561 caagtcatga cgaatgtgct tgcttttggt tagccctaga tcctgcgcta agcgctgata
     4621 aaacagcgtc cactgctcaa ggcttgggga aagctcttgc gctcgtggag catctaacag
     4681 aggggctata taagcagcta gtgctgttaa ttgagatagg gagtctttgg ggtagtctga
     4741 aagcagctca gtatgaccca aagcccaaaa ttgatgtcgg tgatcatcta cagctctatc
     4801 aaagaagact aaagcaccgt gttttggaca atatggcaaa gcgggcaaat accaatctcg
     4861 ttgccaaaag gcttccccat acctatttag ctgaagagca acgcaatcag ggcagtatct
     4921 aaagcggtta tcgctcttaa ctctagaagc agcgactcct agcattaaat gcaccgcacc
     4981 ttgcgcttgg tactccatta accgaatagc ttcgtctcgg cgctccttgc ctacaaacgg
     5041 agcatataaa gggaataagg tatgctcata aataagctgc tgaacagcgt aacgtcctgt
     5101 ttgatgtaga tgtcttgcta tcacacctaa atgcgagggc agacctaagg tagcgaccac
     5161 cttgcggttg ccatacacct catccaacag ctgcttagga ctaacaatcc cttgataaac
     5221 gcctgcccgt gcaatagtgc tataaatcag ctcattcgag tacggaacag gaaagtttct
     5281 catggctaac ctgcttgttt aaataagctc gccatatcca ctattacccc tttccctttt
     5341 aaccgttcat gcatggtttt ttcaggttgg cgttgtgaat agatataacg taaatccgta
     5401 tcaggcaagc tatcccagtc gctgggcttg actaccttta tagccgaaac ctttttactc
     5461 ttggagggct tttctagttc tgacactacc gtttcgcctt ccatcaacca ctgcaaaaca
     5521 agaggcagta acttttgtct tgtcatcgtt ggattctggc taaacgcttt tttaatagtg
     5581 ggaattaaca ggcttgaatc gtaatcctct ttcagcatca gatataaatg acgctgatct
     5641 tcggtatcta actcttgaag ggctttttct tctggtgttt gttcttgtat cgctgcgata
     5701 tctagctgaa gttggattaa ccgtttatca atctcgggaa cgactagatc agaataacga
     5761 gcaatgcgtt ctgggatacc cgagcgtaat gcctctagca tggggtgcac aggctttaac
     5821 tcatcttgat acacttgccg caataaacca gcggtaatac gctcattgcc taaagctagc
     5881 gcacggagct gagcgagtac aaaaagtttt actacaatgt ccatcactcc ttggcttagc
     5941 tcataccaca catcacggac ctcatccgat aacagcgcat ctttgcgttg taaaagctgt
     6001 aattgccaaa gattatccgt aaaagcgatc cactcttgat tgggctttcc acgttgcgtt
     6061 tgttgtatag gatcccagaa tatagctcca aaccctgccc ctctacgtgc agaccgcaaa
     6121 tcagcctcaa aaatctctcg tgctttaggg gtaccaatca acatcactgg tacgccaata
     6181 atattcacca tcgtcacaaa aaagttcagc atctcttgag atccacccga acgagagcgg
     6241 cttaaatgct gaatttcatc aataaccaac aaccctaaag catgtgcatt ggctatttgc
     6301 gacatcaaag ccaacatggt ttctatacca tgacgtttta agccataacg acgctcatag
     6361 ttcgagccca aggctcgatc caacgctctg aaaaaattca agcagatttc ttttagcgaa
     6421 ccattatgcg agcagtctat tttcaaatac accacctgct ctacattgag ttcacgatgg
     6481 taaatcacct gaggatacgt ggctagaata cgatgaagag aggtcgtctt cccactacca
     6541 gaacaaccaa ttaacaataa gctttgtgcc gtagatcgtg cctcctcaaa gcgaaatgtc
     6601 tccaactctc ccgtttgaac acgctcataa ccattttgta aatgcttttg taaatctcct
     6661 gttttaggat ttctgcctac gtagccacct cgaatcatga ccgaaatacg ctcacttagt
     6721 agcaaatgcg tacctaatgg ctgaaaatag tcatctggaa tacgacaaat ggtatgagct
     6781 ctgataacac gggacttttg caagtcagag gaagtaagct gtaaagagga tttcagtgat
     6841 gcagcactat tcactgactc ttgtaatggt ggtaaggcct cgataaaagg attatcacga
     6901 taagcctcta cccccgtatc acgataaact gcttgaatcc gggtagcact catgactcat
     6961 ccttttctgg tggatcctga aataattcag gcacgtatgt gggtaggctg taatcttctt
     7021 gatcatccgc ttccactgcg ttgaaaggaa taactttagc ctcatcacct gaagagcttg
     7081 gcttcaaatg ctccgcacgt tttttacgct ccgaggtcac ggcttctttt ttattagttt
     7141 taatctgctt aatgcgtgtt gatttgggct cagtagtact gggcgttaac ttattcgctt
     7201 tctgaatggt ttgctgaata aacgcctcaa gctccctgcg tttagttaac tcatcctgct
     7261 tcgcattggc tttattgtgt ttttcttgtg cttgtatatc ccaaacctcc caaaatgaga
     7321 gacctttaaa ctgccgacta cgttccgtca gattacagcg ccaaaataca cggctgccaa
     7381 cttgcggaaa caaataaatc gtatcaacca gcactgggtc ataagccgct tctaaatgtt
     7441 gaggtctagc tatatcagtg ctccgctgca accaaccctc acgcagaatc tctgaccccg
     7501 agtaatacaa accccacaaa ttaacgccaa atgaagaaat agagaccttt cggcgaggca
     7561 gtaacgctac tcgcaactgc tcttgctcca cagcccttaa actacctgta cgatgctgca
     7621 taccccattg ccatagctgg acaggaatag acggtaaatc tgtaggaaaa tcagcatctc
     7681 gatcgtattt atccatcacc agatggttat ttctgaataa gatcgtacgc aaaataattt
     7741 gtgtgaactc aaataccgac agagatgcat ctaacctata gtctgtttca ccatggcttt
     7801 tgatccgact gccctctaca atgccaggtg caaaggactt aaactcggct tgtagtgttc
     7861 taaaagtgct ttccactatg cctttagcat cgccacgtct aggtggagca ctttccactc
     7921 gcacattaaa actagaaact aaggcttcga cctgatgact cattaattcg ccacggtccg
     7981 ctagcaacac atctggcaaa cctacacacg gccagtctga gctactaatc tcaatatcat
     8041 gctgggcaca aatggccgtt ttgtcagagc aagcatttac aaaagcctgc atcgccacca
     8101 cataagacgg attttcaaag ccgatataaa agcccgtgat catccgacta aacacatcaa
     8161 tcacaatgta aagcgttggt cttcctatga ttttttggcg atcatgatga tccactaaat
     8221 aaatatcagc aatcgtggca tcaatctcat aacgactccc agggcctaac gcctgagaag
     8281 tggctgtact acttaagggt cgtacgtctt ttttatatac ccctgcttta actctagact
     8341 ttaagcgctg agctttaggg tattctcgat cataaaaata acgaaactga cgtagtgttg
     8401 ggtaatcctc ttggggaatg cgaggaaaat actgagcaaa caagtccaca aatcgtctat
     8461 aggcaacggt ggtctttgta cctttttgat ttaacaggtg cttttctatg gtcaacctaa
     8521 aaaggcgttc aatctcgggc gttaccttgg ttccttcacc cttaccatat tctctggctc
     8581 ggccaatctt tgctgttcct gtcgctgaac gtctttcccc tggtgcaccg ctgtttttgt
     8641 agtcaggaat taatgcatta ggcgtttgac cacgctgcca gtaacggcgt aacaacttat
     8701 aaactgtagc cttagtaacc ttatgttctt ggaccacatg ctcaacgagt tcgcttctga
     8761 ctttagggtc gaaacgatcc ttactattaa taataggaag aatttttcga taatcctcct
     8821 cgcgcttctg gaagctgaca gaatctacag acggctcttc taaatcaaga tgtacataag
     8881 ggtcatcagt tctaactaag cggccttcat ctaaatactg cattagcaac aactcagccc
     8941 tagcttgtgg aactccttta tcagcgctta tttgcatcca gacaacttgg ccatcctcta
     9001 tagccaaaat gcgatacgga tcattatcaa atagcacaac ctcattaatt tgccacatag
     9061 cgcaactcct ccatattcac tacttggcta atacagagat ctgcacactt atttgccctg
     9121 aaagacttat aaatattgaa cttaataaaa ccatttgcgg ttaaggctcg tatctcactc
     9181 aatgttttgc ctaactccaa atcataagca atatcaacct gcttacagac attgataatg
     9241 ttttcatctc ctttttcttg caggatatgg gccaatgggg atagttgtgc taaaagctcc
     9301 gcagaaactt cttctgtttt cactgaataa agccattcaa tattttcttt tactacggga
     9361 tttatttctt tatcagtaaa aatgaaccaa ggaatttgct tttgctgcca atagcgacgc
     9421 tctagttcta gtttttctaa ggtacgctcg tcttgtaagg ctgctgcagg tttgacttga
     9481 atagcaaact gctcaaaagg accatctttg cagtccacta aaaaatcagt agacataacc
     9541 tgatctacac cacgaataac aggatgctta ataccactat ctattgcaat ctgcctggta
     9601 tcactaggta ataaggggaa ctgctcgcgt atatctagca cgctgctctc ccactcaaga
     9661 ctgagaaaaa cagcaagctc taagtcagat agcaaatgat ggactcgtcc cgtcttatga
     9721 gaataaatac ggtgggaacg acctgaagaa ggaacttctt gtactgttag ccatggaata
     9781 tagtctttac catgcccttg gccacgcccc tctttaatac gacgggcaat ttgcacttca
     9841 gaaaatgaag agtttgcttt agccataaca aaagtccagt atgctttttc acagcataac
     9901 tggactgatt tcagtttaca actattctgt ctagtttaag actttattgt catagtttag
     9961 atctattttg ttcagtttaa gactttattg tccgcccaca cccgcttacg cagggcatcc
    10021 atttattact caaccgtaac cgattttgcc aggttacgcg gctggtcaac gtcggtgcct
    10081 ttgatcagcg cgacatggta agccagcagc tgcagcggaa cggtgtagaa gatcggtgca
    10141 atcacctctt ccacatgcgg catctcgacc cgggactagt tggtgtggaa gtcctaatat
    10201 cggtgatcaa tatagtggtt gacatgctgg ctagtcaaca ttgagccttt tgatcatgca
    10261 aatatattac ggtattttac aatcaaatat caaacttaac tattgacttt ataacttatt
    10321 taggtggtaa cattcttata aaaaagaaaa aaattactgc aaaacagtac tagcttttaa
    10381 cttgtatcct aggttatcta tgctgtctca ccatagagaa tattacctat ttcagaatgt
    10441 atgtccatga ttcgccgggt aaatacatat aatacacaaa tctggcttaa taaagtctat
    10501 aatatatctc ataaagaagt gctaaattgg ctagtgctat atatttttaa gaaaatttct
    10561 tttgactaag tccatatcga ctttgtaaaa gttcacatta gcatacatat attacacgag
    10621 ccagaaatag taacttttgc ctaaatcaca aattgcaaaa tttaattgct tgcaaaaggt
    10681 cacatgctta taatcaactt ttttaaaaat ttaaaatact tttttatttt ttatttttaa
    10741 acataaatga aataatttat ttattgttta tgattaccga aacataaaac ctgctcaaga
    10801 aaaagaaact gttttgtcct tggaaaaaaa gcactaccta ggagcggcca aaatgccgag
    10861 gctttcatag cttaaactct ttacagaaaa taggcattat agatcagttc gagttttctt
    10921 attcttcctt ccggttttat cgtcacagtt ttacagtaaa taagtatcac ctcttagagt
    10981 taacctatac taaaatttat gccagttaac tatgagataa gcaagtatca tctcatttca
    11041 ttacctgaag tcgagtaaac agaaaatcca attgttgatg aacctcaatg acttagaact
    11101 atctatcggc agatcatata aagaggattt aggtactcga gaacgctccg tacatgcagc
    11161 taaacctcca agcatctcta acttgggtag tgctttctcc accattttct gtgcttgctc
    11221 cttcgtggca agtccagccc ataatgccca gaatgtagtt gcggattcgt atgacgttct
    11281 gtgcttgatt tttgtgttgt agtcaaagaa aaggtccggt taaacggatc tcgcattgat
    11341 gaggcaacgc taattatcaa catatagatt gttatctatc tgcatgaaca cgaaatcttt
    11401 acttgacgac ttgaggctga tggtgtttat gcaaagaaac cactgtgttt aatatgtgtc
    11461 actgtttgat attactgtca gcgtagaaga taatagtaaa agcggttaat aagtgtattt
    11521 gagataagtg tgataaagtt tttacagcga aaagacgata aatacaagaa aatgattacg
    11581 aggatacgga gagaggtatg tacatgtgta tttatatact aagctgccgg cggttgtttg
    11641 caagaccgag aaaaggctag caagaatcgg gtcattgtag cgtatgcgcc tgtgaacatt
    11701 ctcttcaaca agtttgattc cattgcggtg aaatggtaaa agtcaacccc ctgcgatgta
    11761 tattttcctg tacaatcaat caaaaagcca aatgatttag cattatcttt acatcttgtt
    11821 attttacaga ttttatgttt agatctttta tgcttgcttt tcaaaaggcc tgcaggcaag
    11881 tgcacaaaca atacttaaat aaatactact cagtaataac ctatttctta gcatttttga
    11941 cgaaatttgc tattttgtta gagtctttta caccatttgt ctccacacct ccgcttacat
    12001 caacaccaat aacgccattt aatctaagcg catcaccaac attttctggc gtcagtccac
    12061 cagctaacat aaaatgttgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
    12121 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
    12181 gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta
    12241 gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt
    12301 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
    12361 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
    12421 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
    12481 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
    12541 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
    12601 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
    12661 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
    12721 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
    12781 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
    12841 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
    12901 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
    12961 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
    13021 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
    13081 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
    13141 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
    13201 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
    13261 taaatcaatc taaagtatat atgagtaaac ttggtctgac aggagctcgc tcagaagaac
    13321 tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc
    13381 acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac
    13441 gctatgtcct gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag
    13501 cggccatttt ccaccatgat attcggcaag caggcatcgc catgggtcac gacgagatcc
    13561 tcgccgtcgg gcatccgcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga
    13621 tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc
    13681 tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc
    13741 cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg
    13801 agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg
    13861 tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg
    13921 tcttggagtt cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc
    13981 tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca
    14041 tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca
    14101 atcatgcgaa acgatcctca tcctgtctct tgatcagatc ttgatcccct gcgccatcag
    14161 atccttggcg gcaagaaagc catccagttt actttgcagg gcttcccaac cttaccagag
    14221 ggcgccccag ctggcaattc cggttcgctt gctgtccata aaaccgccca gtctagctat
    14281 cgccatgtaa gcccactgca agctacctgc tttctctttg cgcttgcgtt ttcccttgtc
    14341 cagatagccc agtagctgac attcatccgg gcgatcgatg gaagccggcg gcacctcgct
    14401 aacggattca ccactccaag aattggagcc aatcaattct tgcggagaac tgtgaatgcg
    14461 caaaccaacc cttggcagaa catatccatc gcgtccgcca tctccagcag ccgcacgcgg
    14521 cgcatcgggg gggggggggg gtttcaattc atcatttttt ttttattctt ttttttgatt
    14581 tcggtttcct tgaaattttt ttgattcggt aatctccgaa cagaaggaag aacgaaggaa
    14641 ggagcacaga cttagattgg tatatatacg catatgtagt gttgaagaaa catgaaattg
    14701 cccagtattc ttaacccaac tgcacagaac aaaaacctgc aggaaacgaa gataaatcat
    14761 gtcgaaagct acatataagg aacgtgctgc tactcatcct agtcctgttg ctgccaagct
    14821 atttaatatc atgcacgaaa agcaaacaaa cttgtgtgct tcattggatg ttcgtaccac
    14881 caaggaatta ctggagttag ttgaagcatt aggtcccaaa atttgtttac taaaaacaca
    14941 tgtggatatc ttgactgatt tttccatgga gggcacagtt aagccgctaa aggcattatc
    15001 cgccaagtac aattttttac tcttcgaaga cagaaaattt gctgacattg gtaatacagt
    15061 caaattgcag tactctgcgg gtgtatacag aatagcagaa tgggcagaca ttacgaatgc
    15121 acacggtgtg gtgggcccag gtattgttag cggtttgaag caggcggcag aagaagtaac
    15181 aaaggaacct agaggccttt tgatgttagc agaattgtca tgcaagggct ccctatctac
    15241 tggagaatat actaagggta ctgttgacat tgcgaagagc gacaaagatt ttgttatcgg
    15301 ctttattgct caaagagaca tgggtggaag agatgaaggt tacgattggt tgattatgac
    15361 acccggtgtg ggtttagatg acaagggaga cgcattgggt caacagtata gaaccgtgga
    15421 tgatgtggtc tctacaggat ctgacattat tattgttgga agaggactat ttgcaaaggg
    15481 aagggatgct aaggtagagg gtgaacgtta cagaaaagca ggctgggaag catatttgag
    15541 aagatgcggc cagcaaaact aaaaaactgt attataagta aatgcatgta tactaaactc
    15601 acaaattaga gcttcaattt aattatatca gttattaccc gggaatctcg gtcgtaatga
    15661 tttttataat gacgaaaaaa aaaaaattgg aaagaaaacc cccccccccc gcagcgttgg
    15721 gtcctggcca cgggtgcgca tgatcgtgct cctgtcgttg aggacccggc taggctggcg
    15781 gggttgcctt actggttagc agaatgaatc accgatacgc gagcgaacgt gaagcgactg
    15841 ctgctgcaaa acgtctgcga cctgagcaac aacatgaatg gtcttcggtt tccgtgtttc
    15901 gtaaagtctg gaaacgcgga agtcagcgcc ctgcaccatt atgttccgga tctgcatcgc
    15961 aggatgctgc tggctaccct gtggaacacc tacatctgta ttaacgaagc gctggcattg
    16021 accctgagtg atttttctct ggtcccgccg catccatacc gccagttgtt taccctcaca
    16081 acgttccagt aaccgggcat gttcatcatc agtaacccgt atcgtgagca tcctctctcg
    16141 tttcatcggt atcattaccc ccatgaacag aaatccccct tacacggagg catcagtgac
    16201 caaacaggaa aaaaccgccc ttaacatggc ccgctttatc agaagccaga cattaacgct
    16261 tctggagaaa ctcaacgagc tggacgcgga tgaacaggca gacatctgtg aatcgcttca
    16321 cgaccacgct gatgagcttt accgctgggc cattctcatg aagaatatct tgaatttatt
    16381 gtcatatta
//
