LOCUS       sequence-420609-        4112 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4112)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 4112)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_SARS-CoV-1-Nucleocapsid
FEATURES             Location/Qualifiers
     source          1..4112
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    33..132
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1455..1554)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1571..1590)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1572..1590)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1595..1611)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1595..1611)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2042..2833
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2926..3514
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3415..3434
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3668..3685
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3844..3871)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3963..4049)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     join(4098..4112,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(4112,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtctt aagctcgggc cccaaataat gattttattt tgactgatag
       61 tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca actttgtaca
      121 aaaaagcaga agggccgtca aggccaggat ccatgggatc ctctgataat ggaccccaat
      181 caaaccaacg tagtgccccc cgcattacat ttggtggacc cacagattca actgacaata
      241 accagaatgg aggacgcaat ggggcaaggc caaaacagcg ccgaccccaa ggtttaccca
      301 ataatactgc gtcttggttc acagctctca ctcagcatgg caaggaggaa cttagattcc
      361 ctcgaggcca gggcgttcca atcaacacca atagtggtcc agatgaccaa attggctact
      421 accgaagagc tacccgacga gttcgtggtg gtgacggcaa aatgaaagag ctcagcccca
      481 gatggtactt ctattaccta ggaactggcc cagaagcttc acttccctac ggcgctaaca
      541 aagaaggcat cgtatgggtt gcaactgagg gagccttgaa tacacccaaa gaccacattg
      601 gcacccgcaa tcctaataac aatgctgcca ccgtgctaca acttcctcaa ggaacaacat
      661 tgccaaaagg cttctacgca gagggaagca gaggcggcag tcaagcctct tctcgctcct
      721 catcacgtag tcgcggtaat tcaagaaatt caactcctgg cagcagtagg ggaaattctc
      781 ctgctcgaat ggctagcgga ggtggtgaaa ctgccctcgc gctattgctg ctagacagat
      841 tgaaccagct tgagagcaaa gtttctggta aaggccaaca acaacaaggc caaactgtca
      901 ctaagaaatc tgctgctgag gcatctaaaa agcctcgcca aaaacgtact gccacaaaac
      961 agtacaacgt cactcaagca tttgggagac gtggtccaga acaaacccaa ggaaatttcg
     1021 gggaccaaga cctaatcaga caaggaactg attacaaaca ttggccgcaa attgcacaat
     1081 ttgctccaag tgcctctgca ttctttggaa tgtcacgcat tggcatggaa gtcacacctt
     1141 cgggaacatg gctgacttat catggagcca ttaaattgga tgacaaagat ccacaattca
     1201 aagacaacgt catactgctg aacaagcaca ttgacgcata caaaacattc ccaccaacag
     1261 agcctaaaaa ggacaaaaag aaaaagactg atgaagctca gcctttgccg cagagacaaa
     1321 agaagcagcc cactgtgact cttcttcctg cggctgacat ggatgatttc tccagacaac
     1381 ttcaaaattc catgagtgga gcttctgctg attcaactca ggcacttaat taagacgcga
     1441 tcgcggcctc atgggcccag ctttcttgta caaagttggc attataaaaa ataattgctc
     1501 atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc
     1561 agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg
     1621 gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgcct
     1681 cctctagacc agccaggaca gaaatgcctc gacttcgctg ctacccaagg ttgccgggtg
     1741 acgcacaccg tggaaacgga tgaaggcacg aacccagtgg acataagcct gttcggttcg
     1801 taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg
     1861 cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttggggt
     1921 acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat
     1981 gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat
     2041 tatgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat
     2101 cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg
     2161 cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa ggcttgatga
     2221 aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag
     2281 cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg
     2341 ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg
     2401 tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga
     2461 acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca
     2521 ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc
     2581 tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg
     2641 caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta
     2701 tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc
     2761 ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt
     2821 agtcggcaaa taaccctcga gccacccatg accaaaatcc cttaacgtga gttacgcgtc
     2881 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     2941 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     3001 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     3061 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     3121 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     3181 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     3241 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     3301 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     3361 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     3421 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     3481 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     3541 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     3601 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     3661 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     3721 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     3781 gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc caggaagagt ttgtagaaac
     3841 gcaaaaaggc catccgtcag gatggccttc tgcttagttt gatgcctggc agtttatggc
     3901 gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc
     3961 ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag
     4021 tcttccgact gagcctttcg ttttatttga tgcctggcag ttccctactc tcgcgttaac
     4081 gctagcatgg atgttttccc agtcacgacg tt
//
