LOCUS       sequence-421067-       11194 bp    DNA     circular SYN 15-APR-2025
DEFINITION  genomic integration of genes (inserted in I-SceI-site) via 
            transposon Tn5 with GmR and mTagBFP2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11194)
  TITLE     HOLD-Jaeger Lab pYT plasmids
REFERENCE   2  (bases 1 to 11194)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pYTRW15K_2G5
FEATURES             Location/Qualifiers
     source          1..11194
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1689..2387
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     promoter        join(2568..2573,2574..2590,2591..2596)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             2785..3318
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     misc_feature    5493..5785
                     /label=CEN4
                     /note="S. cerevisiae CEN4 centromere"
     rep_origin      6433..6886
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1"
     primer_bind     complement(6914..6936)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7022..7162
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7077..7096
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7177..7194)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7348..7936)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7428..7447)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8115..8909)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8226..8245)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     8836..8855
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        9348..9563
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             9564..10367
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
ORIGIN
        1 ctagtcccgg gtccacatat ccacgggctg gcaagggagc gcagcgaccg cgcagggcga
       61 agcccggaga gcaagcccgt agggcgccgc agccgccgta ggcggtcacg actttgcgaa
      121 gcaaagtcta gtgagtatac tcaagcattg agtggcccgc cggaggcacc gccttgcgct
      181 gcccccgtcg agccggttgg acaccaaaag ggaggggcag gcatggcggc atacgcgatc
      241 atgcgatgca agaagctggc gaaaatgggc aacgtggcgg ccagtctcaa gcacgcctac
      301 cgcgagcgcg agacgcccaa cgctgacgcc agcaggacgc cagagaacga gcactgggcg
      361 gccagcagca ccgatgaagc gatgggccga ctgcgcgagt tgctgccaga gaagcggcgc
      421 aaggacgctg tgttggcggt cgagtacgtc atgacggcca gcccggaatg gtggaagtcg
      481 gccagccaag aacagcaggc ggcgttcttc gagaaggcgc acaagtggct ggcggacaag
      541 tacggggcgg atcgcatcgt gacggccagc atccaccgtg acgaaaccag cccgcacatg
      601 accgcgttcg tggtgccgct gacgcaggac ggcaggctgt cggccaagga gttcatcggc
      661 aacaaagcgc agatgacccg cgaccagacc acgtttgcgg ccgctgtggc cgatctaggg
      721 ctgcaacggg gcatcgaggg cagcaaggca cgtcacacgc gcattcaggc gttctacgag
      781 gccctggagc ggccaccagt gggccacgtc accatcagcc cgcaagcggt cgagccacgc
      841 gcctatgcac cgcagggatt ggccgaaaag ctgggaatct caaagcgcgt tgagacgccg
      901 gaagccgtgg ccgaccggct gacaaaagcg gttcggcagg ggtatgagcc tgccctacag
      961 gccgccgcag gagcgcgtga gatgcgcaag aaggccgatc aagcccaaga gacggcccga
     1021 gaccttcggg agcgcctgaa gcccgttctg gacgccctgg ggccgttgaa tcgggatatg
     1081 caggccaagg ccgccgcgat catcaaggcc gtgggcgaaa agctgctgac ggaacagcgg
     1141 gaagtccagc gccagaaaca ggcccagcgc cagcaggaac gcgggcgcgc acatttcccc
     1201 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gactgactct tatacacaag
     1261 ttatctgcag cactcaacag atctccgtct acttgcgaca cctctcttaa ggtagctgga
     1321 ccggattcgg gtctagccta tatcgtcaat ccgttgatct atgtcgggtg cggagaaaga
     1381 ggtaatgaaa tgggcgatcg cccacagctt acacagacct aatcgtagag ttcctagtaa
     1441 tacttagtgg ctcgttacct cgttcttcca ggccaggggg ccgtacgcgc tcggcgaaca
     1501 gtataagcag attcgactca ctagggataa cagggtaatc tctgggtatc gactcaatct
     1561 cgcgctattg tgctatatcc gtggacggtt gcggtcaatt ctgtcggaaa atcaacggat
     1621 acgaacaatt actcgcgact tcctcaatac gtgctgcaac gcgcgcgaac tttagaagga
     1681 gatataccat gagcgagctg attaaggaga acatgcacat gaagctgtac atggagggca
     1741 ccgtggacaa ccatcacttc aagtgcacat ccgagggcga aggcaagccc tacgagggca
     1801 cccagaccat gagaatcaag gtggtcgagg gcggccctct ccccttcgcc ttcgacatcc
     1861 tggctactag cttcctctac ggcagcaaga ccttcatcaa ccacacccag ggcatccccg
     1921 acttcttcaa gcagtccttc cctgagggct tcacatggga gagagtcacc acatacgaag
     1981 acgggggcgt gctgaccgct acccaggaca ccagcctcca ggacggctgc ctcatctaca
     2041 acgtcaagat cagaggggtg aacttcacat ccaacggccc tgtgatgcag aagaaaacac
     2101 tcggctggga ggccttcacc gagacgctgt accccgctga cggcggcctg gaaggcagaa
     2161 acgacatggc cctgaagctc gtgggcggga gccatctgat cgcaaacgcc aagaccacat
     2221 atagatccaa gaaacccgct aagaacctca agatgcctgg cgtctactat gtggactaca
     2281 gactggaaag aatcaaggag gccaacaacg agacctacgt cgagcagcac gaggtggcag
     2341 tggccagata ctgcgacctc cctagcaaac tggggcacaa gcttaatctc gagtaacgcg
     2401 cgcgtaacta taacggtcct aaggtagcga gcttacttgt gctgttgtgt aaggacacgc
     2461 ttaacccaga gaattccagc gacctattct acgactacgc gtccatggct cgaggagctc
     2521 ggtaccgccc cactacccga tttatcaaga tttggcggag agtcccattg acataagcct
     2581 gttcggttcg taaactgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct
     2641 tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt
     2701 tttttgtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat
     2761 gtttgatgtt atggagcagc aacgatgtta cgcagcagca acgatgttac gcagcagggc
     2821 agtcgcccta aaacaaagtt aggtggctca agtatgggca tcattcgcac atgtaggctc
     2881 ggccctgacc aagtcaaatc catgcgggct gctcttgatc ttttcggtcg tgagttcgga
     2941 gacgtagcca cctactccca acatcagccg gactccgatt acctcgggaa cttgctccgt
     3001 agtaagacat tcatcgcgct tgctgccttc gaccaagaag cggttgttgg cgctctcgcg
     3061 gcttacgttc tgcccaagtt tgagcagccg cgtagtgaga tctatatcta tgatctcgca
     3121 gtctccggcg agcaccggag gcagggcatt gccaccgcgc tcatcaatct cctcaagcat
     3181 gaggccaacg cgcttggtgc ttatgtgatc tacgtgcaag cagattacgg tgacgatccc
     3241 gcagtggctc tctatacaaa gttgggcata cgggaagaag tgatgcactt tgatatcgac
     3301 ccaagtaccg ccacctaagt cgactggcaa acagctatta tgggtattat gggtgtgtat
     3361 ccagaacgac ggatcttgac gcaatttggc gtggctctct taaggtagcc ctccgaccaa
     3421 ttgaggtgcg ctctgaccga tcagtttaga cttgtgtata agagtcagag cgtcctgaac
     3481 ggaacctttc ccgttttcca ggatctgatc ttccatgtga cctcctaaca tggtaacgtt
     3541 catgataact tctgctcttc atcgtgcggc cgactgggct aaatctgtgt tctcttcggc
     3601 ggcgctgggt gatcctcgcc gtactgcccg cttggttaac gtcgccgccc aattggcaaa
     3661 atattctggt aaatcaataa ccatctcatc agagggtagt gaagccatgc aggaaggcgc
     3721 ttaccgattt atccgcaatc ccaacgtttc tgccgaggcg atcagaaagg ctggcgccat
     3781 gcaaacagtc aagttggctc aggagtttcc cgaactgctg gccattgagg acaccacctc
     3841 tttgagttat cgccaccagg tcgccgaaga gcttggcaag ctgggctcta ttcaggataa
     3901 atcccgcgga tggtgggttc actccgttct cttgctcgag gccaccacat tccgcaccgt
     3961 aggattactg catcaggagt ggtggatgcg cccggatgac cctgccgatg cggatgaaaa
     4021 ggagagtggc aaatggctgg cagcggccgc aactagccgg ttacgcatgg gcagcatgat
     4081 gagcaacgtg attgcggtct gtgaccgcga agccgatatt catgcttatc tgcaggacaa
     4141 actggcgcat aacgagcgct tcgtggtgcg ctccaagcac ccacgcaagg acgtagagtc
     4201 tgggttgtat ctgtacgacc atctgaagaa ccaaccggag ttgggtggct atcagatcag
     4261 cattccgcaa aagggcgtgg tggataaacg cggtaaacgt aaaaatcgac cagcccgcaa
     4321 ggcgagcttg agcctgcgca gtgggcgcat cacgctaaaa caggggaata tcacgctcaa
     4381 cgcggtgctg gccgaggaga ttaacccgcc caagggtgag accccgttga aatggttgtt
     4441 gctgaccagc gaaccggtcg agtcgctagc ccaagccttg cgcgtcatcg acatttatac
     4501 ccatcgctgg cggatcgagg agttccataa ggcatggaaa accggagcag gagccgagag
     4561 gcaacgcatg gaggagccgg ataatctgga gcggatggtc tcgatcctct cgtttgttgc
     4621 ggtcaggctg ttacagctca gagaaagctt cacgctgccg caagcactca gggcgcaagg
     4681 gctgctaaag gaagcggaac acgtagaaag ccagtccgca gaaacggtgc tgaccccgga
     4741 tgaatgtcag ctactgggct atctggacaa gggaaaacgc aagcgcaaag agaaagcagg
     4801 tagcttgcag tgggcttaca tggcgatagc tagactgggc ggttttatgg acagcaagcg
     4861 aaccggaatt gccagctggg gcgccctctg ggaaggttgg gaagccctgc aaagtaaact
     4921 ggatggcttt cttgccgcca aggatctgat ggcgcagggg atcaagatct gacccgggac
     4981 tagttggtgt ggaagtccta atatcggtga tcaatatagt ggttgacatg ctggctagtc
     5041 aacattgagc cttttgatca tgcaaatata ttacggtatt ttacaatcaa atatcaaact
     5101 taactattga ctttataact tatttaggtg gtaacattct tataaaaaag aaaaaaatta
     5161 ctgcaaaaca gtactagctt ttaacttgta tcctaggtta tctatgctgt ctcaccatag
     5221 agaatattac ctatttcaga atgtatgtcc atgattcgcc gggtaaatac atataataca
     5281 caaatctggc ttaataaagt ctataatata tctcataaag aagtgctaaa ttggctagtg
     5341 ctatatattt ttaagaaaat ttcttttgac taagtccata tcgactttgt aaaagttcac
     5401 attagcatac atatattaca cgagccagaa atagtaactt ttgcctaaat cacaaattgc
     5461 aaaatttaat tgcttgcaaa aggtcacatg cttataatca acttttttaa aaatttaaaa
     5521 tactttttta ttttttattt ttaaacataa atgaaataat ttatttattg tttatgatta
     5581 ccgaaacata aaacctgctc aagaaaaaga aactgttttg tccttggaaa aaaagcacta
     5641 cctaggagcg gccaaaatgc cgaggctttc atagcttaaa ctctttacag aaaataggca
     5701 ttatagatca gttcgagttt tcttattctt ccttccggtt ttatcgtcac agttttacag
     5761 taaataagta tcacctctta gagttaacct atactaaaat ttatgccagt taactatgag
     5821 ataagcaagt atcatctcat ttcattacct gaagtcgagt aaacagaaaa tccaattgtt
     5881 gatgaacctc aatgacttag aactatctat cggcagatca tataaagagg atttaggtac
     5941 tcgagaacgc tccgtacatg cagctaaacc tccaagcatc tctaacttgg gtagtgcttt
     6001 ctccaccatt ttctgtgctt gctccttcgt ggcaagtcca gcccataatg cccagaatgt
     6061 agttgcggat tcgtatgacg ttctgtgctt gatttttgtg ttgtagtcaa agaaaaggtc
     6121 cggttaaacg gatctcgcat tgatgaggca acgctaatta tcaacatata gattgttatc
     6181 tatctgcatg aacacgaaat ctttacttga cgacttgagg ctgatggtgt ttatgcaaag
     6241 aaaccactgt gtttaatatg tgtcactgtt tgatattact gtcagcgtag aagataatag
     6301 taaaagcggt taataagtgt atttgagata agtgtgataa agtttttaca gcgaaaagac
     6361 gataaataca agaaaatgat tacgaggata cggagagagg tatgtacatg tgtatttata
     6421 tactaagctg ccggcggttg tttgcaagac cgagaaaagg ctagcaagaa tcgggtcatt
     6481 gtagcgtatg cgcctgtgaa cattctcttc aacaagtttg attccattgc ggtgaaatgg
     6541 taaaagtcaa ccccctgcga tgtatatttt cctgtacaat caatcaaaaa gccaaatgat
     6601 ttagcattat ctttacatct tgttatttta cagattttat gtttagatct tttatgcttg
     6661 cttttcaaaa ggcctgcagg caagtgcaca aacaatactt aaataaatac tactcagtaa
     6721 taacctattt cttagcattt ttgacgaaat ttgctatttt gttagagtct tttacaccat
     6781 ttgtctccac acctccgctt acatcaacac caataacgcc atttaatcta agcgcatcac
     6841 caacattttc tggcgtcagt ccaccagcta acataaaatg ttgcctcgcg cgtttcggtg
     6901 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     6961 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     7021 gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc
     7081 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
     7141 aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     7201 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     7261 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     7321 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     7381 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     7441 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     7501 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     7561 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     7621 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     7681 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     7741 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     7801 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     7861 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     7921 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     7981 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     8041 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     8101 tgacaggagc tcgctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa
     8161 tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct
     8221 tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg
     8281 ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca
     8341 tcgccatggg tcacgacgag atcctcgccg tcgggcatcc gcgccttgag cctggcgaac
     8401 agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg
     8461 gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag
     8521 gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg
     8581 gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag
     8641 tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc
     8701 agccacgata gccgcgctgc ctcgtcttgg agttcattca gggcaccgga caggtcggtc
     8761 ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag
     8821 ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa
     8881 cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatca
     8941 gatcttgatc ccctgcgcca tcagatcctt ggcggcaaga aagccatcca gtttactttg
     9001 cagggcttcc caaccttacc agagggcgcc ccagctggca attccggttc gcttgctgtc
     9061 cataaaaccg cccagtctag ctatcgccat gtaagcccac tgcaagctac ctgctttctc
     9121 tttgcgcttg cgttttccct tgtccagata gcccagtagc tgacattcat ccgggcgatc
     9181 gatggaagcc ggcggcacct cgctaacgga ttcaccactc caagaattgg agccaatcaa
     9241 ttcttgcgga gaactgtgaa tgcgcaaacc aacccttggc agaacatatc catcgcgtcc
     9301 gccatctcca gcagccgcac gcggcgcatc gggggggggg gggggntttc aattcatcat
     9361 ttttttttta ttcttttttt tgatttcggt ttccttgaaa tttttttgat tcggtaatct
     9421 ccgaacagaa ggaagaacga aggaaggagc acagacttag attggtatat atacgcatat
     9481 gtagtgttga agaaacatga aattgcccag tattcttaac ccaactgcac agaacaaaaa
     9541 cctgcaggaa acgaagataa atcatgtcga aagctacata taaggaacgt gctgctactc
     9601 atcctagtcc tgttgctgcc aagctattta atatcatgca cgaaaagcaa acaaacttgt
     9661 gtgcttcatt ggatgttcgt accaccaagg aattactgga gttagttgaa gcattaggtc
     9721 ccaaaatttg tttactaaaa acacatgtgg atatcttgac tgatttttcc atggagggca
     9781 cagttaagcc gctaaaggca ttatccgcca agtacaattt tttactcttc gaagacagaa
     9841 aatttgctga cattggtaat acagtcaaat tgcagtactc tgcgggtgta tacagaatag
     9901 cagaatgggc agacattacg aatgcacacg gtgtggtggg cccaggtatt gttagcggtt
     9961 tgaagcaggc ggcagaagaa gtaacaaagg aacctagagg ccttttgatg ttagcagaat
    10021 tgtcatgcaa gggctcccta tctactggag aatatactaa gggtactgtt gacattgcga
    10081 agagcgacaa agattttgtt atcggcttta ttgctcaaag agacatgggt ggaagagatg
    10141 aaggttacga ttggttgatt atgacacccg gtgtgggttt agatgacaag ggagacgcat
    10201 tgggtcaaca gtatagaacc gtggatgatg tggtctctac aggatctgac attattattg
    10261 ttggaagagg actatttgca aagggaaggg atgctaaggt agagggtgaa cgttacagaa
    10321 aagcaggctg ggaagcatat ttgagaagat gcggccagca aaactaaaaa actgtattat
    10381 aagtaaatgc atgtatacta aactcacaaa ttagagcttc aatttaatta tatcagttat
    10441 tacccgggaa tctcggtcgt aatgattttt ataatgacga aaaaaaaaaa attggaaaga
    10501 aaaccccccc cccccscagc gttgggtcct ggccacgggt gcgcatgatc gtgctcctgt
    10561 cgttgaggac ccggctaggc tggcggggtt gccttactgg ttagcagaat gaatcaccga
    10621 tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga gcaacaacat
    10681 gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac gcggaagtca gcgccctgca
    10741 ccattatgtt ccggatctgc atcgcaggat gctgctggct accctgtgga acacctacat
    10801 ctgtattaac gaagcgctgg cattgaccct gagtgatttt tctctggtcc cgccgcatcc
    10861 ataccgccag ttgtttaccc tcacaacgtt ccagtaaccg ggcatgttca tcatcagtaa
    10921 cccgtatcgt gagcatcctc tctcgtttca tcggtatcat tacccccatg aacagaaatc
    10981 ccccttacac ggaggcatca gtgaccaaac aggaaaaaac cgcccttaac atggcccgct
    11041 ttatcagaag ccagacatta acgcttctgg agaaactcaa cgagctggac gcggatgaac
    11101 aggcagacat ctgtgaatcg cttcacgacc acgctgatga gctttaccgc tgggccattc
    11161 tcatgaagaa tatcttgaat ttattgtcat atta
//
