LOCUS       sequence-421068-       11717 bp    DNA     circular SYN 15-APR-2025
DEFINITION  genomic integration of genes (inserted in I-SceI-site) via 
            transposon Tn5 with TcR and mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11717)
  TITLE     HOLD-Jaeger Lab pYT plasmids
REFERENCE   2  (bases 1 to 11717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pYTRW18K_3T5
FEATURES             Location/Qualifiers
     source          1..11717
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1689..2399
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1834..1852)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2102..2121
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        join(2576..2581,2582..2598,2599..2604)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             2652..3842
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(3004..3023)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     misc_feature    6017..6309
                     /label=CEN4
                     /note="S. cerevisiae CEN4 centromere"
     rep_origin      6957..7410
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1"
     primer_bind     complement(7438..7460)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7546..7686
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7601..7620
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7701..7718)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7872..8460)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7952..7971)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8639..9433)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8750..8769)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     9360..9379
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        9872..10087
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             10088..10891
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
ORIGIN
        1 ctagtcccgg gtccacatat ccacgggctg gcaagggagc gcagcgaccg cgcagggcga
       61 agcccggaga gcaagcccgt agggcgccgc agccgccgta ggcggtcacg actttgcgaa
      121 gcaaagtcta gtgagtatac tcaagcattg agtggcccgc cggaggcacc gccttgcgct
      181 gcccccgtcg agccggttgg acaccaaaag ggaggggcag gcatggcggc atacgcgatc
      241 atgcgatgca agaagctggc gaaaatgggc aacgtggcgg ccagtctcaa gcacgcctac
      301 cgcgagcgcg agacgcccaa cgctgacgcc agcaggacgc cagagaacga gcactgggcg
      361 gccagcagca ccgatgaagc gatgggccga ctgcgcgagt tgctgccaga gaagcggcgc
      421 aaggacgctg tgttggcggt cgagtacgtc atgacggcca gcccggaatg gtggaagtcg
      481 gccagccaag aacagcaggc ggcgttcttc gagaaggcgc acaagtggct ggcggacaag
      541 tacggggcgg atcgcatcgt gacggccagc atccaccgtg acgaaaccag cccgcacatg
      601 accgcgttcg tggtgccgct gacgcaggac ggcaggctgt cggccaagga gttcatcggc
      661 aacaaagcgc agatgacccg cgaccagacc acgtttgcgg ccgctgtggc cgatctaggg
      721 ctgcaacggg gcatcgaggg cagcaaggca cgtcacacgc gcattcaggc gttctacgag
      781 gccctggagc ggccaccagt gggccacgtc accatcagcc cgcaagcggt cgagccacgc
      841 gcctatgcac cgcagggatt ggccgaaaag ctgggaatct caaagcgcgt tgagacgccg
      901 gaagccgtgg ccgaccggct gacaaaagcg gttcggcagg ggtatgagcc tgccctacag
      961 gccgccgcag gagcgcgtga gatgcgcaag aaggccgatc aagcccaaga gacggcccga
     1021 gaccttcggg agcgcctgaa gcccgttctg gacgccctgg ggccgttgaa tcgggatatg
     1081 caggccaagg ccgccgcgat catcaaggcc gtgggcgaaa agctgctgac ggaacagcgg
     1141 gaagtccagc gccagaaaca ggcccagcgc cagcaggaac gcgggcgcgc acatttcccc
     1201 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gactgactct tatacacaag
     1261 ttatctgcag cactcaacag atctccgtct acttgcgaca cctctcttaa ggtagctgga
     1321 ccggattcgg gtctagccta tatcgtcaat ccgttgatct atgtcgggtg cggagaaaga
     1381 ggtaatgaaa tgggcgatcg cccacagctt acacagacct aatcgtagag ttcctagtaa
     1441 tacttagtgg ctcgttacct cgttcttcca ggccaggggg ccgtacgcgc tcggcgaaca
     1501 gtataagcag attcgactca ctagggataa cagggtaatc tctgggtatc gactcaatct
     1561 cgcgctattg tgctatatcc gtggacggtt gcggtcaatt ctgtcggaaa atcaacggat
     1621 acgaacaatt actcgcgact tcctcaatac gtgctgcaac gcgcgcgaac tttagaagga
     1681 gatataccat ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc
     1741 gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg
     1801 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc
     1861 ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg
     1921 tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt
     1981 gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctcct
     2041 tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg
     2101 gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg
     2161 aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact
     2221 acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct
     2281 acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac
     2341 agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtaac
     2401 gcgcgcgtaa ctataacggt cctaaggtag cgagcttact tgtgctgttg tgtaaggaca
     2461 cgcttaaccc agagaattcc agcgacctat tctacgacta cgcgtccatg gctcgaggag
     2521 ctcggtaccg ccccactacc cgatttatca agatttggcg gagagtccct catgtttgac
     2581 agcttatcat cgataagctt taatgcggta gtttatcaca gttaaattgc taacgcagtc
     2641 aggcaccgtg tatgaaatct aacaatgcgc tcatcgtcat cctcggcacc gtcaccctgg
     2701 atgctgtagg cataggcttg gttatgccgg tactgccggg cctcttgcgg gatatcgtcc
     2761 attccgacag catcgccagt cactatggcg tgctgctagc gctatatgcg ttgatgcaat
     2821 ttctatgcgc acccgttctc ggagcactgt ccgaccgctt tggccgccgc ccagtcctgc
     2881 tcgcttcgct acttggagcc actatcgact acgcgatcat ggcgaccaca cccgtcctgt
     2941 ggatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg
     3001 gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga
     3061 gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca
     3121 tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg
     3181 gctgcttcct aatgcaggag tcgcataagg gagagcgtcg accgatgccc ttgagagcct
     3241 tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga
     3301 ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg gtcattttcg
     3361 gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa
     3421 tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga
     3481 agcaggccat tatcgccggc atggcggccg acgcgctggg ctacgtcttg ctggcgttcg
     3541 cgacgcgagg ctggatggcc ttccccatta tgattcttct cgcttccggc ggcatcggga
     3601 tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga cgaccatcag ggacagcttc
     3661 aaggatcgct cgcggctctt accagcctaa cttcgatcat tggaccgctg atcgtcacgg
     3721 cgatttatgc cgcctcggcg agcacatgga acgggttggc atggattgta ggcgccgccc
     3781 tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg gagccgggcc acctcgacct
     3841 gagtcgactg gcaaacagct attatgggta ttatgggtgt gtatccagaa cgacggatct
     3901 tgacgcaatt tggcgtggct ctcttaaggt agccctccga ccaattgagg tgcgctctga
     3961 ccgatcagtt tagacttgtg tataagagtc agagcgtcct gaacggaacc tttcccgttt
     4021 tccaggatct gatcttccat gtgacctcct aacatggtaa cgttcatgat aacttctgct
     4081 cttcatcgtg cggccgactg ggctaaatct gtgttctctt cggcggcgct gggtgatcct
     4141 cgccgtactg cccgcttggt taacgtcgcc gcccaattgg caaaatattc tggtaaatca
     4201 ataaccatct catcagaggg tagtgaagcc atgcaggaag gcgcttaccg atttatccgc
     4261 aatcccaacg tttctgccga ggcgatcaga aaggctggcg ccatgcaaac agtcaagttg
     4321 gctcaggagt ttcccgaact gctggccatt gaggacacca cctctttgag ttatcgccac
     4381 caggtcgccg aagagcttgg caagctgggc tctattcagg ataaatcccg cggatggtgg
     4441 gttcactccg ttctcttgct cgaggccacc acattccgca ccgtaggatt actgcatcag
     4501 gagtggtgga tgcgcccgga tgaccctgcc gatgcggatg aaaaggagag tggcaaatgg
     4561 ctggcagcgg ccgcaactag ccggttacgc atgggcagca tgatgagcaa cgtgattgcg
     4621 gtctgtgacc gcgaagccga tattcatgct tatctgcagg acaaactggc gcataacgag
     4681 cgcttcgtgg tgcgctccaa gcacccacgc aaggacgtag agtctgggtt gtatctgtac
     4741 gaccatctga agaaccaacc ggagttgggt ggctatcaga tcagcattcc gcaaaagggc
     4801 gtggtggata aacgcggtaa acgtaaaaat cgaccagccc gcaaggcgag cttgagcctg
     4861 cgcagtgggc gcatcacgct aaaacagggg aatatcacgc tcaacgcggt gctggccgag
     4921 gagattaacc cgcccaaggg tgagaccccg ttgaaatggt tgttgctgac cagcgaaccg
     4981 gtcgagtcgc tagcccaagc cttgcgcgtc atcgacattt atacccatcg ctggcggatc
     5041 gaggagttcc ataaggcatg gaaaaccgga gcaggagccg agaggcaacg catggaggag
     5101 ccggataatc tggagcggat ggtctcgatc ctctcgtttg ttgcggtcag gctgttacag
     5161 ctcagagaaa gcttcacgct gccgcaagca ctcagggcgc aagggctgct aaaggaagcg
     5221 gaacacgtag aaagccagtc cgcagaaacg gtgctgaccc cggatgaatg tcagctactg
     5281 ggctatctgg acaagggaaa acgcaagcgc aaagagaaag caggtagctt gcagtgggct
     5341 tacatggcga tagctagact gggcggtttt atggacagca agcgaaccgg aattgccagc
     5401 tggggcgccc tctgggaagg ttgggaagcc ctgcaaagta aactggatgg ctttcttgcc
     5461 gccaaggatc tgatggcgca ggggatcaag atctgacccg ggactagttg gtgtggaagt
     5521 cctaatatcg gtgatcaata tagtggttga catgctggct agtcaacatt gagccttttg
     5581 atcatgcaaa tatattacgg tattttacaa tcaaatatca aacttaacta ttgactttat
     5641 aacttattta ggtggtaaca ttcttataaa aaagaaaaaa attactgcaa aacagtacta
     5701 gcttttaact tgtatcctag gttatctatg ctgtctcacc atagagaata ttacctattt
     5761 cagaatgtat gtccatgatt cgccgggtaa atacatataa tacacaaatc tggcttaata
     5821 aagtctataa tatatctcat aaagaagtgc taaattggct agtgctatat atttttaaga
     5881 aaatttcttt tgactaagtc catatcgact ttgtaaaagt tcacattagc atacatatat
     5941 tacacgagcc agaaatagta acttttgcct aaatcacaaa ttgcaaaatt taattgcttg
     6001 caaaaggtca catgcttata atcaactttt ttaaaaattt aaaatacttt tttatttttt
     6061 atttttaaac ataaatgaaa taatttattt attgtttatg attaccgaaa cataaaacct
     6121 gctcaagaaa aagaaactgt tttgtccttg gaaaaaaagc actacctagg agcggccaaa
     6181 atgccgaggc tttcatagct taaactcttt acagaaaata ggcattatag atcagttcga
     6241 gttttcttat tcttccttcc ggttttatcg tcacagtttt acagtaaata agtatcacct
     6301 cttagagtta acctatacta aaatttatgc cagttaacta tgagataagc aagtatcatc
     6361 tcatttcatt acctgaagtc gagtaaacag aaaatccaat tgttgatgaa cctcaatgac
     6421 ttagaactat ctatcggcag atcatataaa gaggatttag gtactcgaga acgctccgta
     6481 catgcagcta aacctccaag catctctaac ttgggtagtg ctttctccac cattttctgt
     6541 gcttgctcct tcgtggcaag tccagcccat aatgcccaga atgtagttgc ggattcgtat
     6601 gacgttctgt gcttgatttt tgtgttgtag tcaaagaaaa ggtccggtta aacggatctc
     6661 gcattgatga ggcaacgcta attatcaaca tatagattgt tatctatctg catgaacacg
     6721 aaatctttac ttgacgactt gaggctgatg gtgtttatgc aaagaaacca ctgtgtttaa
     6781 tatgtgtcac tgtttgatat tactgtcagc gtagaagata atagtaaaag cggttaataa
     6841 gtgtatttga gataagtgtg ataaagtttt tacagcgaaa agacgataaa tacaagaaaa
     6901 tgattacgag gatacggaga gaggtatgta catgtgtatt tatatactaa gctgccggcg
     6961 gttgtttgca agaccgagaa aaggctagca agaatcgggt cattgtagcg tatgcgcctg
     7021 tgaacattct cttcaacaag tttgattcca ttgcggtgaa atggtaaaag tcaaccccct
     7081 gcgatgtata ttttcctgta caatcaatca aaaagccaaa tgatttagca ttatctttac
     7141 atcttgttat tttacagatt ttatgtttag atcttttatg cttgcttttc aaaaggcctg
     7201 caggcaagtg cacaaacaat acttaaataa atactactca gtaataacct atttcttagc
     7261 atttttgacg aaatttgcta ttttgttaga gtcttttaca ccatttgtct ccacacctcc
     7321 gcttacatca acaccaataa cgccatttaa tctaagcgca tcaccaacat tttctggcgt
     7381 cagtccacca gctaacataa aatgttgcct cgcgcgtttc ggtgatgacg gtgaaaacct
     7441 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     7501 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca
     7561 gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta
     7621 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     7681 atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     7741 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     7801 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     7861 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     7921 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     7981 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     8041 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     8101 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     8161 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     8221 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     8281 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     8341 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     8401 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     8461 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     8521 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     8581 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag gagctcgctc
     8641 agaagaactc gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga gcggcgatac
     8701 cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag ctcttcagca atatcacggg
     8761 tagccaacgc tatgtcctga tagcggtccg ccacacccag ccggccacag tcgatgaatc
     8821 cagaaaagcg gccattttcc accatgatat tcggcaagca ggcatcgcca tgggtcacga
     8881 cgagatcctc gccgtcgggc atccgcgcct tgagcctggc gaacagttcg gctggcgcga
     8941 gcccctgatg ctcttcgtcc agatcatcct gatcgacaag accggcttcc atccgagtac
     9001 gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg
     9061 tatgcagccg ccgcattgca tcagccatga tggatacttt ctcggcagga gcaaggtgag
     9121 atgacaggag atcctgcccc ggcacttcgc ccaatagcag ccagtccctt cccgcttcag
     9181 tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg
     9241 ctgcctcgtc ttggagttca ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg
     9301 ggcgcccctg cgctgacagc cggaacacgg cggcatcaga gcagccgatt gtctgttgtg
     9361 cccagtcata gccgaatagc ctctccaccc aagcggccgg agaacctgcg tgcaatccat
     9421 cttgttcaat catgcgaaac gatcctcatc ctgtctcttg atcagatctt gatcccctgc
     9481 gccatcagat ccttggcggc aagaaagcca tccagtttac tttgcagggc ttcccaacct
     9541 taccagaggg cgccccagct ggcaattccg gttcgcttgc tgtccataaa accgcccagt
     9601 ctagctatcg ccatgtaagc ccactgcaag ctacctgctt tctctttgcg cttgcgtttt
     9661 cccttgtcca gatagcccag tagctgacat tcatccgggc gatcgatgga agccggcggc
     9721 acctcgctaa cggattcacc actccaagaa ttggagccaa tcaattcttg cggagaactg
     9781 tgaatgcgca aaccaaccct tggcagaaca tatccatcgc gtccgccatc tccagcagcc
     9841 gcacgcggcg catcgggggg gggggggggn tttcaattca tcattttttt tttattcttt
     9901 tttttgattt cggtttcctt gaaatttttt tgattcggta atctccgaac agaaggaaga
     9961 acgaaggaag gagcacagac ttagattggt atatatacgc atatgtagtg ttgaagaaac
    10021 atgaaattgc ccagtattct taacccaact gcacagaaca aaaacctgca ggaaacgaag
    10081 ataaatcatg tcgaaagcta catataagga acgtgctgct actcatccta gtcctgttgc
    10141 tgccaagcta tttaatatca tgcacgaaaa gcaaacaaac ttgtgtgctt cattggatgt
    10201 tcgtaccacc aaggaattac tggagttagt tgaagcatta ggtcccaaaa tttgtttact
    10261 aaaaacacat gtggatatct tgactgattt ttccatggag ggcacagtta agccgctaaa
    10321 ggcattatcc gccaagtaca attttttact cttcgaagac agaaaatttg ctgacattgg
    10381 taatacagtc aaattgcagt actctgcggg tgtatacaga atagcagaat gggcagacat
    10441 tacgaatgca cacggtgtgg tgggcccagg tattgttagc ggtttgaagc aggcggcaga
    10501 agaagtaaca aaggaaccta gaggcctttt gatgttagca gaattgtcat gcaagggctc
    10561 cctatctact ggagaatata ctaagggtac tgttgacatt gcgaagagcg acaaagattt
    10621 tgttatcggc tttattgctc aaagagacat gggtggaaga gatgaaggtt acgattggtt
    10681 gattatgaca cccggtgtgg gtttagatga caagggagac gcattgggtc aacagtatag
    10741 aaccgtggat gatgtggtct ctacaggatc tgacattatt attgttggaa gaggactatt
    10801 tgcaaaggga agggatgcta aggtagaggg tgaacgttac agaaaagcag gctgggaagc
    10861 atatttgaga agatgcggcc agcaaaacta aaaaactgta ttataagtaa atgcatgtat
    10921 actaaactca caaattagag cttcaattta attatatcag ttattacccg ggaatctcgg
    10981 tcgtaatgat ttttataatg acgaaaaaaa aaaaattgga aagaaaaccc ccccccccgc
    11041 agcgttgggt cctggccacg ggtgcgcatg atcgtgctcc tgtcgttgag gacccggcta
    11101 ggctggcggg gttgccttac tggttagcag aatgaatcac cgatacgcga gcgaacgtga
    11161 agcgactgct gctgcaaaac gtctgcgacc tgagcaacaa catgaatggt cttcggtttc
    11221 cgtgtttcgt aaagtctgga aacgcggaag tcagcgccct gcaccattat gttccggatc
    11281 tgcatcgcag gatgctgctg gctaccctgt ggaacaccta catctgtatt aacgaagcgc
    11341 tggcattgac cctgagtgat ttttctctgg tcccgccgca tccataccgc cagttgttta
    11401 ccctcacaac gttccagtaa ccgggcatgt tcatcatcag taacccgtat cgtgagcatc
    11461 ctctctcgtt tcatcggtat cattaccccc atgaacagaa atccccctta cacggaggca
    11521 tcagtgacca aacaggaaaa aaccgccctt aacatggccc gctttatcag aagccagaca
    11581 ttaacgcttc tggagaaact caacgagctg gacgcggatg aacaggcaga catctgtgaa
    11641 tcgcttcacg accacgctga tgagctttac cgctgggcca ttctcatgaa gaatatcttg
    11701 aatttattgt catatta
//
