LOCUS       sequence-421287-        3881 bp    DNA     circular SYN 15-APR-2025
DEFINITION  C2_Pyrearinus termitilluminans click beetle luciferase ('Eluc' 
            version - human-codon optimized).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3881)
  TITLE     Reprogrammable small molecule synthetic sensors of endogeous 
            metabolic pathways in C4 grass model, Setaria viridis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pDL0_212
FEATURES             Location/Qualifiers
     source          1..3881
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(24..42)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        110..214
                     /gene="bla"
                     /label=AmpR promoter
     CDS             436..1227
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1398..1986
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1887..1906
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2140..2157
                     /label=L4440
                     /note="L4440 vector, forward primer"
     RBS             3709..3717
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 gtggctcttc agtggacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
       61 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      121 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      181 ataaatgctt caataatatt gaaaaaggaa gagtatgcgc tcacgcaact ggtccagaac
      241 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
      301 tttttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
      361 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
      421 acaaagttaa acatcatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
      481 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
      541 tccgcagtgg atggcggcct gaagccacac agcgatattg atttgctggt tacggtgacc
      601 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
      661 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
      721 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
      781 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
      841 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
      901 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
      961 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     1021 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     1081 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     1141 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccatta cgtaaaaggc
     1201 gagatcacca aggtagtcgg caaataactg tcagaccaag tttactcata tatactttag
     1261 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     1321 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     1381 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     1441 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     1501 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     1561 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     1621 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     1681 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     1741 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     1801 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     1861 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     1921 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     1981 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     2041 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     2101 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     2161 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatca
     2221 ctctgtggtc tcaggtggcg agagagagaa gaacgtggtg tacggccccg agcccaagca
     2281 ccctctgggc aacttcaccg ccggcgagat gctgtacaac gctctgcaca agcactccca
     2341 catcccccag gccatcctgg acgtgatggg caacgagtcc ctttcctacc aggagttctt
     2401 cgacactact gtgaagctgg gccagagcct ccagaactgt ggctacaaga tgaacgatgt
     2461 cgtgtcgatc tgtgcagaga acaacaagag attcttcatc cccatcatct ccgcctggta
     2521 catcggcatg gtggtggccc ctgtgaacga ggactatatc ccagacgagc tgtgtaaagt
     2581 gaccggcatc tccaagccga tcctggtctt taccactagg aagatcctgc ctaaggtttt
     2641 ggaggttaaa gacagaacca actacataaa gaggatcatc atactggact ctgaagagaa
     2701 cctgctgggc tgcgagagcc tgcacaactt catgtccagg tactccgaca acaacctcca
     2761 aacattcaag cctctgcact acgaccctgt ggaccaggta gccgccatcc tgtgctcctc
     2821 cggcacaacc ggcctgccta aaggcgtgat gcagacccac aggaacatct gtgtgagact
     2881 cacacacgca tctgacccca gagtgggtac acaactcatc cccggcgtat ccgtgctggc
     2941 ctacctgcca ttcttccacg ccttcggctt cagtatcaac ctgggctatt tcatggtggg
     3001 cctgagagtg gtgatgctcc gaaggtttaa ccaggaggtg ttcctgaagg ccatccagga
     3061 ctacgaggtg aggagcgtga tcaacgttcc ctccacaatc ctgttcctgt ccaagagccc
     3121 tctggtggac aagtacgacc tatccaccct ggcggagctg tgctgtggag ccgctcctct
     3181 ggcgaaggag gtggccgaga tcgccgtgaa gaggctgaac ctgccaggga tacggtgtgg
     3241 ctacggtcta acagagtcta cctccgccaa catccatact ctgcacaacg agttcaagtc
     3301 cggctccctg ggcaaggtga caccttacat ggccgccaag atcatcgaca ggaacaccgg
     3361 cgaggccctg ggtccaaacc aggtgggcga gctgtgcatc tggggaccta tggtaacaaa
     3421 aggctatgtg aacaacccac aggctactaa ggaggccatc gacgacgacg gctggctgca
     3481 ctctggcgac ttcggctact acgacgagga cgagtatttc tacatcgtgg accggtacaa
     3541 ggagctgatc aaatacaagg gctatcaggt cgcccctgtg gagctggagg agatcctcct
     3601 tcagcaccca ggcatcaggg acgtggccgt cgtgggtatc cctgacatcg aggccggcga
     3661 gctgccagcc ggcttcgtgg tgaagcagcc cggcgcccaa ctcaccgcta aggaggtgta
     3721 cgacttcctg gcccagaggg tgtctcactc caagtacctg aggggcggcg taaggttcgt
     3781 ggactctatc cccaggaacg tgacaggcaa gattagtcga aaagagctga gggaggccct
     3841 gatggagaag gcttctaagc tttgagcttt gagaccacga a
//
