LOCUS       sequence-421765-        2157 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Encodes the S. cerevisiae HSP150 Translational Fusion Partner 
            Sequence as a Type 3a' part to be used in the yeast 
            secretion/display (YSD) extension of the MoClo yeast toolkit (YTK).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   O'Riordan NM, Juric V, O'Neill SK, Roche AP, Young PW
  TITLE     A Yeast Modular Cloning (MoClo) Toolkit Expansion for Optimization 
            of Heterologous Protein Secretion and Surface Display in 
            Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2024 Mar 14. doi: 10.1021/acssynbio.3c00743.
  PUBMED    38483353
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2024 Mar 14. doi: 10.1021/acssynbio.3c00743."
COMMENT     Sequence Label: pYSD042
FEATURES             Location/Qualifiers
     source          1..2157
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(522..556)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(627..1286)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1201..1220
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1468..2056
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1957..1976
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcat atgcaataca aaaagacttt ggttgcctct gctttggccg ctactacatt
       61 ggccgcctat gctccatctg agccttggtc cactttgact ccaacagcca cttacagcgg
      121 tggtgttacc gactacgctt ccaccttcgg tattgccgtt caaccaatct ccactacatc
      181 cagcgcatca tctgcagcca ccacagcctc atctaaggcc aagagagctg cttcccaaat
      241 tggtgatggt caagtccaag ctgctaccac tactgcttct gtctctacca agagtaccgc
      301 tgccgccgtt tctcagatcg gtgatggtca aatccaagct actactaaga ctaccgctgc
      361 tgctgtctct caaattggtg atggtcaaat tcaagctacc accaagacta cctctgctaa
      421 gactaccgcc gctgccgttt ctcaaatcag tgatggtcaa atccaagcta ccaccactac
      481 tttagcccca aagctagaca aacgtgcttg agaccagacc aataaaaaac gcccggcggc
      541 aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga tctatcaaca
      601 ggagtccaag cgagctcgat atcaaattac gccccgccct gccactcatc gcagtactgt
      661 tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga
      721 atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg
      781 ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag
      841 ggattggctg aaacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt
      901 tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg
      961 tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg
     1021 tgaacactat cccatatcac cagctcaccg tctttcattg ccatacgaaa ttccggatga
     1081 gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc
     1141 tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga
     1201 gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg
     1261 gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac
     1321 tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg
     1381 tgcccgatca atcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     1441 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     1501 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     1561 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     1621 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     1681 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     1741 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     1801 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     1861 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     1921 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     1981 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     2041 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     2101 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtag
//
