LOCUS       sequence-421922-        9822 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expression of base editor dCas12a-A3A-N57G in mammalian cells for 
            base editing, along with non-fused EGFP fluorescence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9822)
  AUTHORS   Chen F, Lian M, Ma B, Gou S, Luo X, Yang K, Shi H, Xie J, Ge W, 
            Ouyang Z, Lai C, Li N, Zhang Q, Jin Q, Liang Y, Chen T, Wang J, Zhao
            X, Li L, Yu M, Ye Y, Wang K, Wu H, Lai L
  TITLE     Multiplexed base editing through Cas12a variant-mediated cytosine 
            and adenine base editors.
  JOURNAL   Commun Biol. 2022 Nov 2;5(1):1163. doi: 10.1038/s42003-022-04152-8.
  PUBMED    36323848
REFERENCE   2  (bases 1 to 9822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-11-2"; volume: "5(1):1163. doi"; pages: " 
            10.1038/s42003-022-04152-8"
COMMENT     Sequence Label: pCMV-dCas12a-A3A-N57G
FEATURES             Location/Qualifiers
     source          1..9822
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        132..335
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     286..306
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     377..396
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        377..395
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      403..412
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             409..1005
                     /codon_start=1
                     /gene="human APOBEC3A"
                     /gene_synonym="A3A"
                     /product="human cytidine deaminase (C to U editing enzyme) 
                     that efficiently edits methylated cytosines (Wang et al., 
                     2018)"
                     /label=APOBEC3A
                     /note="also known as hA3A"
                     /translation="MEASPASGPRHLMDPHIFTSNFNNGIGRHKTYLCYEVERLDNGTS
                     VKMDQHRGFLHGQAKNLLCGFYGRHAELRFLDLVPSLQLDPAQIYRVTWFISWSPCFSW
                     GCAGEVRAFLQENTHVRLRIFAARIYDYDPLYKEALQMLRDAGAQVSIMTYDEFKHCWD
                     TFVDHQGCPFQPWDGLDEHSQALSGRLRAILQNQGN"
     CDS             4744..4764
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4777..5025
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5038..5058
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5065..5118
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5119..5367
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5368..5388
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5389..5445
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5446..5694
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5695..5715
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5716..5769
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5770..6018
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6031..6051
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6061..6120
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             join(6121..6123,6124..6837)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(6121..6123,6124..6834)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(6163..6184)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6424..6443)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6771..6792
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(6877..6894)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6883..7107
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7178..7194)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7178..7194)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7191..7213)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7202..7218
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7226..7232,7233..7250,7251..7256))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7271..7292
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7409..7426)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7580..8168)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7660..7679)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8339..9130,9131..9199))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8962..8981
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9200..9304)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9383..9402)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(9574..9822,1..131)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat ggaagccagc
      421 ccagcatccg ggcccagaca cttgatggat ccacacatat tcacttccaa ctttaacaat
      481 ggcattggaa ggcataagac ctacctgtgc tacgaagtgg agcgcctgga caatggcacc
      541 tcggtcaaga tggaccagca caggggcttt ctacacggcc aggctaagaa tcttctctgt
      601 ggcttttacg gccgccatgc ggagctgcgc ttcttggacc tggttccttc tttgcagttg
      661 gacccggccc agatctacag ggtcacttgg ttcatctcct ggagcccctg cttctcctgg
      721 ggctgtgccg gggaagtgcg tgcgttcctt caggagaaca cacacgtgag actgcgtatc
      781 ttcgctgccc gcatctatga ttacgacccc ctatataagg aggcactgca aatgctgcgg
      841 gatgctgggg cccaagtctc catcatgacc tacgatgaat ttaagcactg ctgggacacc
      901 tttgtggacc accagggatg tcccttccag ccctgggatg gactagatga gcacagccaa
      961 gccctgagtg ggaggctgcg ggccattctc cagaatcagg gaaacagcgg cagcgagact
     1021 cccgggacct cagagtccgc cacacccgaa agtatgtcaa agctggagaa attcaccaac
     1081 tgttatagcc tgtctaagac cctgcgcttc aaggcaatcc cagtgggcaa gacacaagag
     1141 aacattgaca acaaacggct cctggtggag gatgagaaga gggctgaaga ttacaagggc
     1201 gttaagaagc tgctggatag gtactatctg tcattcatca acgatgtcct ccacagtatc
     1261 aagctgaaga atctgaacaa ttacatttct ctgttccgga agaagacacg gaccgagaag
     1321 gagaacaaag agctggagaa tctggagatc aacctgagga aagaaatagc taaggctttc
     1381 aaagggaacg agggttacaa gtccctgttc aagaaagaca ttatcgagac tattctgcct
     1441 gagttcctgg acgataaaga tgagatcgcc ctcgtcaatt ccttcaatgg gtttaccaca
     1501 gcctttaccg gcttcttcga caatagagag aatatgttct ctgaagaggc caaatccact
     1561 agcatcgcct ttcgctgcat aaacgagaac ctgactaggt acatcagcaa tatggacatc
     1621 tttgagaaag tcgatgccat attcgacaaa catgaggtgc aggagattaa ggagaagatc
     1681 ctgaactcag attacgatgt cgaagatttc ttcgagggag agttcttcaa cttcgtgctc
     1741 acacaagagg gcattgatgt gtacaatgca atcattggag ggttcgtgac agagagtggc
     1801 gagaagataa agggcctgaa cgagtatatc aacctctaca accagaaaac caagcagaaa
     1861 ctgcctaagt tcaagccact gtacaaacaa gtgctctcag atagggaaag cctgagcttc
     1921 tacggtgaag ggtatacatc agatgaagaa gtgctcgaag tgttccgcaa caccctcaat
     1981 aagaacagtg aaatcttctc ttcaatcaag aagctggaga aactgttcaa gaatttcgat
     2041 gagtactcct ctgccggaat ctttgtgaag aatggccctg caatatccac tattagcaaa
     2101 gacatctttg gcgagtggaa cgttatcagg gataagtgga atgccgagta cgatgatatt
     2161 catctcaaga agaaagccgt ggttacagag aaatacgagg atgatagacg caagagcttt
     2221 aagaagattg gtagcttctc tctcgaacag ctgcaggagt acgccgacgc tgacctgtca
     2281 gtcgtggaga aactcaagga gatcataatc cagaaggtgg atgaaatcta caaagtgtat
     2341 ggaagctctg agaaactctt cgatgcagac tttgttctgg agaagagtct gaagaagaac
     2401 gacgcagtgg ttgctatcat gaaggacctg ctggattctg ttaagtcttt cgagaattac
     2461 attaaggcat tctttggtga agggaaggag acaaataggg acgagagctt ctatggcgac
     2521 tttgttctgg cctacgacat cctcctcaag gttgaccaca tctatgacgc tatacggaat
     2581 tacgttaccc agaagcccta tagcaaagac aagttcaagc tgtatttcca gaatccacag
     2641 tttatgggtg ggtgggataa agacaaagaa acagattaca gggccactat cctgcggtac
     2701 ggcagcaaat actatctggc tatcatggat aagaagtacg ccaaatgcct ccagaagatc
     2761 gacaaggacg acgtgaacgg taactacgag aagatcaatt acaagctcct gccaggacct
     2821 aacaagatgc tgcccaaggt gttcttctcc aagaaatgga tggcctacta taacccaagc
     2881 gaggacattc agaagatata caagaatggg acattcaaga agggcgatat gttcaacctc
     2941 aacgactgcc acaagctgat tgatttcttc aaggatagca tttctcgcta tcccaagtgg
     3001 tctaatgcat acgatttcaa cttcagcgag actgagaagt acaaagacat cgctggcttc
     3061 taccgggagg tggaagagca aggctataag gtgtcattcg aatccgcttc taagaaggaa
     3121 gtggataagc tcgtggaaga gggtaagctg tacatgttcc agatatacaa caaagacttc
     3181 agcgataaga gccacggcac tccaaacctc catactatgt atttcaagct gctgtttgac
     3241 gagaacaacc acggacagat taggctgtca ggaggcgcag aactcttcat gcgcagagct
     3301 tcactgaaga aggaggaact cgttgtccac ccagccaata gccctatagc caataagaat
     3361 ccagacaatc ctaagaaaac cactactctg tcttacgatg tgtataagga taagagattc
     3421 tctgaagatc agtacgaact gcacataccc attgccatta acaagtgccc taagaacatc
     3481 ttcaagatta acacagaggt tagagtgctc ctgaaacacg acgataaccc ttatgttata
     3541 ggcattgctc gcggagagag aaacctgctg tacatcgtcg tggtggacgg caaaggcaac
     3601 atcgtggaac agtacagtct caatgaaatc attaacaatt tcaacggaat ccgcattaag
     3661 accgactacc attctctcct cgacaagaag gagaaagaaa ggttcgaagc aagacagaat
     3721 tggacaagta tagagaatat caaagaactg aaggctgggt acatctctca ggttgtgcac
     3781 aagatatgtg agctggtgga gaagtacgac gctgttatcg ccctcgcgga cctgaatagc
     3841 ggcttcaaga actccagggt gaaggtggag aagcaggtgt atcagaagtt cgagaagatg
     3901 ctgatcgaca agctcaacta tatggtggac aagaaatcca atccttgcgc tactggtgga
     3961 gccctgaagg gctatcaaat caccaataag ttcgaatctt tcaagtctat gagcacccag
     4021 aatggcttca tcttctacat acccgcatgg ctgacatcca agattgatcc ctctaccgga
     4081 tttgttaatc tgctcaagac taagtacacc tctattgctg actcaaagaa gttcatatca
     4141 tcatttgacc gcatcatgta cgtgccagaa gaggacctgt tcgagtttgc cctggattac
     4201 aagaatttct ctcggactga cgccgactac atcaagaagt ggaagctcta ctcttatggt
     4261 aatcggattc gcatattccg caatcccaag aagaataacg tgttcgattg ggaggaagtt
     4321 tgcctcacca gcgcttacaa ggagctgttc aataagtatg ggattaacta ccagcagggc
     4381 gacataagag ccctgctgtg cgaacaatct gataaggcat tctattcctc tttcatggca
     4441 ctgatgtcac tgatgctgca aatgcgcaat tccatcaccg gaagaacaga cgtggccttt
     4501 ctgatctctc ctgtcaagaa ctcagatggc atcttctacg attcccgcaa ctatgaagca
     4561 caggagaatg ctatcctgcc taagaatgcc gatgcaaatg gagcctataa catcgccaga
     4621 aaggtcctct gggccatagg acaattcaag aaagctgaag atgagaagct ggacaaggtg
     4681 aagatcgcca tttcaaacaa agagtggctc gaatatgctc agacctcagt gaagcatgga
     4741 tcacccaaga agaaacggaa agtgtctggt ggttctacta atctgtcaga tattattgaa
     4801 aaggagaccg gtaagcaact ggttatccag gaatccatcc tcatgctccc agaggaggtg
     4861 gaagaagtca ttgggaacaa gccggaaagc gatatactcg tgcacaccgc ctacgacgag
     4921 agcaccgacg agaatgtcat gcttctgact agcgacgccc ctgaatacaa gccttgggct
     4981 ctggtcatac aggatagcaa cggtgagaac aagattaaga tgctctctgg tggttctccc
     5041 aagaagaaga ggaaagtcgg gcccgagggc agaggaagtc tgctaacatg cggtgacgtc
     5101 gaggagaatc ctggcccaac taatctgtca gatattattg aaaaggagac cggtaagcaa
     5161 ctggttatcc aggaatccat cctcatgctc ccagaggagg tggaagaagt cattgggaac
     5221 aagccggaaa gcgatatact cgtgcacacc gcctacgacg agagcaccga cgagaatgtc
     5281 atgcttctga ctagcgacgc ccctgaatac aagccttggg ctctggtcat acaggatagc
     5341 aacggtgaga acaagattaa gatgctcccc aagaagaaga ggaaagtcgc tactaacttc
     5401 agcctgctga agcaggctgg agacgtggag gagaaccctg gacctaccaa cctgtccgat
     5461 atcattgaga aagagaccgg caaacagctg gtgatccagg agagcatcct gatgctgccc
     5521 gaagaggtgg aggaagtgat cggcaacaag cccgagtccg acatcctggt gcacacagcc
     5581 tatgatgaat ccaccgacga gaacgtgatg ctgctgacct ccgatgctcc cgagtataaa
     5641 ccctgggcac tggtgatcca ggactctaat ggagagaaca agatcaagat gctgcccaag
     5701 aagaagagga aagtcgaagg acggggctcc ctcctgacct gtggcgatgt ggaagagaac
     5761 cccggcccca caaacctcag tgacattatc gagaaggaaa caggaaaaca gctcgtcatt
     5821 caagaatcta ttcttatgtt gcctgaggaa gtcgaagagg ttattggcaa taaacctgaa
     5881 tctgatattc ttgtccatac cgcttacgat gagtccacag atgaaaatgt tatgctgctc
     5941 acatctgacg caccagagta caaaccatgg gcgctcgtta ttcaagattc caacggcgaa
     6001 aacaaaatca aaatgctttc tggtggttct cctaaaaaaa aaagaaaggt gggctcgggc
     6061 cagtgtacta attatgctct cttgaaattg gctggagatg ttgagagcaa cccaggtccc
     6121 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     6181 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     6241 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     6301 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag
     6361 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     6421 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     6481 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     6541 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
     6601 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     6661 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     6721 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     6781 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaaatc
     6841 atcaccatca ccattgagtt taaacccgct gatcagcctc gactgtgcct tctagttgcc
     6901 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     6961 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     7021 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     7081 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcga
     7141 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     7201 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     7261 agggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     7321 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg ggaagaggcg
     7381 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     7441 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     7501 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     7561 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     7621 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     7681 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     7741 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     7801 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     7861 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     7921 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     7981 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     8041 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     8101 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     8161 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     8221 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     8281 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     8341 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     8401 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     8461 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     8521 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     8581 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     8641 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     8701 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     8761 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     8821 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     8881 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     8941 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     9001 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     9061 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     9121 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     9181 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     9241 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     9301 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat cgatctcccg
     9361 atcccctagg gtcgactctc agtacaatct gctctgatgc cgcatagtta agccagtatc
     9421 tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa
     9481 caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc
     9541 tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact agttattaat
     9601 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
     9661 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     9721 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     9781 atttacggta aactgcccac ttggcagtac atcaagtgta tc
//
