LOCUS       sequence-422105-        6258 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expresses noro-VLP decorated with influenza M2e in insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6258)
  AUTHORS   Lampinen V, Grohn S, Soppela S, Blazevic V, Hytonen VP, Hankaniemi 
            MM
  TITLE     SpyTag/SpyCatcher display of influenza M2e peptide on norovirus-like
            particle provides stronger immunization than direct genetic fusion.
  JOURNAL   Front Cell Infect Microbiol. 2023 Jun 22;13:1216364. doi: 
            10.3389/fcimb.2023.1216364. eCollection 2023.
  PUBMED    37424789
REFERENCE   2  (bases 1 to 6258)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Cell 
            Infect Microbiol."; date: "2023-06-22"; volume: "13:1216364. doi"; 
            pages: " 10.3389/fcimb.2023.1216364. eCollection 2023"
COMMENT     Sequence Label: M2e-noro-VLP
FEATURES             Location/Qualifiers
     source          1..6258
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             949..1073
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     promoter        complement(1616..1725)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     promoter        1855..1946
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     1871..1888
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     3'UTR           3677..4049
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     primer_bind     complement(4401..4418)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4511..5099)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4591..4610)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5270..6061,6062..6130))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5893..5912
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6131..6235)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattggcgg aaggccgtca
      361 aggccgtgac gttaaaacta ttaagccatc caatcgaccg ttagtcgaat caggaccgct
      421 ggtgcgagaa gccgcgaagt atggcgaatg catcgtataa cgtgtggagt ccgctcatta
      481 gagcgtcatg tttagacaag aaagctacat atttaattga tcccgatgat tttattgata
      541 aattgaccct aactccatac acggtattct acaatggcgg ggttttggtc aaaatttccg
      601 gactgcgatt gtacatgctg ttaacggctc cgcccactat taatgaaatt aaaaattcca
      661 attttaaaaa acgcagcaag agaaacattt gtatgaaaga atgcgtagaa ggaaagaaaa
      721 atgtcgtgga catgctgaac aacaagatta atatgcctcc gtgtataaaa aaaatattga
      781 acgatttgaa agaaaacaat gtaccgcgcg gcggtatgta caggaagagg tttatactaa
      841 actgttacat tgcaaacgtg gtttcgtgtg ccaagtgtga aaaccgatgt ttaatcaagg
      901 ctctgacgca tttctacaac cacgactcca agtgtgtggg tgaagtcatg catcttttaa
      961 tcaaatccca agatgtgtat aaaccaccaa actgccaaaa aatgaaaact gtggacaagc
     1021 tctgtccgtt tgctggcaac tgcaagggtc tcaatcctat ttgtaattat tgaataataa
     1081 aacaattata aatgctaaat ttgtttttta ttaacgatac aaaccaaacg caacaagaac
     1141 atttgtagta ttatctataa ttgaaaacgc gtagttataa tcgctgaggt aatatttaaa
     1201 atcattttca aatgattcac agttaatttg cgacaatata attttatttt cacataaact
     1261 agacgccttg tcgtcttctt cttcgtattc cttctctttt tcatttttct cctcataaaa
     1321 attaacatag ttattatcgt atccatatat gtatctatcg tatagagtaa attttttgtt
     1381 gtcataaata tatatgtctt ttttaatggg gtgtatagta ccgctgcgca tagtttttct
     1441 gtaatttaca acagtgctat tttctggtag ttcttcggag tgtgttgctt taattattaa
     1501 atttatataa tcaatgaatt tgggatcgtc ggttttgtac aatatgttgc ctctagaata
     1561 ctgcagtgag cggccgccaa aggcctgttg agctctatga attctagaga tctgtgattg
     1621 taaataaaat gtaatttaca gtatagtatt ttaattaata tacaaatgat ttgataataa
     1681 ttcttattta actataatat attgtgttgg gttgaattaa aggtcccggc atcctcaaat
     1741 gcataatttc atagtccccc ttgttgtaag tgatgcgtat ttctgaatct ttgtaaaata
     1801 gcacacaaga ctccaacgcg tttggcgttt tattttcttg ctcgactcta ggatatcatg
     1861 gagataatta aaatgataac catctcgcaa ataaataagt attttactgt tttcgtaaca
     1921 gttttgtaat aaaaaaacct ataaatatag gatccatgaa gatggcttcc agcgacgcta
     1981 acccttccga cggttctgct gctaacctgg tgcctgaagt gaacaacgaa gtgatggctc
     2041 tggaacccgt cgtgggtgct gctattgctg ctcctgtggc tggacagcag aacgtgatcg
     2101 acccctggat tcgtaacaac ttcgtgcagg ctcccggtgg cgagttcact gttagtccta
     2161 gaaacgctcc cggcgagatc ctttggagtg ctcctttggg tcccgacttg aacccctacc
     2221 tgtctcacct ggctcgtatg tacaacggtt acgctggtgg tttcgaggtg caagtgatcc
     2281 tggctggaaa cgctttcacc gctggcaaag tgatcttcgc tgctgtgcct cctaacttcc
     2341 ctaccgaggg actgtcccct agccaagtga ctatgttccc tcacatcgtg gtggacgtgc
     2401 gtcagttgga acctgtgctg atccctctgc ctgacgtgcg caacaacttc taccactaca
     2461 accagtctaa cgaccccacc atcaagctga tcgctatgct gtacacccct ctgcgtgcta
     2521 acaacgctgg cgacgacgtg ttcactgtgt cctgcagagt gctgacccgt ccttctcctg
     2581 acttcgactt catcttcctg gtgccaccta ccgtcgagtc ccgtaccaag cctttctctg
     2641 tgcctgtcct gaccgtggaa gagatgacca actctcgttt ccccattccg ctggaaaagt
     2701 tgttcaccgg tccatcctcc gctttcgtgg tgcaacctca gaacggtcgt tgcactactg
     2761 acggtgtcct gctgggaact acccagctgt ctcctgtgaa catctgcacc ttcagaggcg
     2821 acgtgaccca catcaccggt tctcgtaact acaccatgaa cctggcttcc cagaactgga
     2881 acgactacga ccctaccgaa gagatccccg ctcctctggg tactccagac ttcgtgggaa
     2941 agatccaggg tgtcctgact cagaccactc gtaccgatgg ttccaccagg ggtcacaagg
     3001 ctaccgtgta cactggttcc gctgacttcg ctcctaagct gggtcgtgtg cagttcgaaa
     3061 ccgacaccga ccgtgacttc gaggctaacc agaacaccaa gttcacccct gtgggtgtca
     3121 tccaggacgg tggtactact caccgtaacg agcctcagca gtgggtcctg ccttcctact
     3181 ctggtcgtaa cacccacaac gtgcacttgg ctccagctgt ggctccaact ttcccaggcg
     3241 agcaactgct gttcttccgt tccaccatgc ctggttgctc tggttacccc aacatggacc
     3301 tggactgcct gctgcctcaa gagtgggtgc agtacttcta tcaagaggct gctcccgctc
     3361 agtctgatgt ggctctgttg cgtttcgtga accccgacac cggtcgtgtc ttgttcgagt
     3421 gcaagctgca caagtccggt tacgtgaccg tggctcacac tggtcaacac gacctggtca
     3481 tccctccaaa cggttacttc cgtttcgact cctgggtcaa ccagttctac accctggctc
     3541 ctatgggcaa cggaactggt agacgtcgtg ctgtgactag tggtggtatg tctttgctca
     3601 cggaggtgga gacacccatc aggaacgagt ggggttgccg atgtaatgac tcctcggatt
     3661 aaaagcttat taataaaaca cgatacattg ttattagtac atttattaag cgctagattc
     3721 tgtgcgttgt tgatttacag acaattgttg tacgtatttt aataattcat taaatttata
     3781 atctttaggg tggtatgtta gagcgaaaat caaatgattt tcagcgtctt tatatctgaa
     3841 tttaaatatt aaatcctcaa tagatttgta aaataggttt cgattagttt caaacaaggg
     3901 ttgtttttcc gaaccgatgg ctggactatc taatggattt tcgctcaacg ccacaaaact
     3961 tgccaaatct tgtagcagca atctagcttt gtcgatattc gtttgtgttt tgttttgtaa
     4021 taaaggttcg acgtcgttca aaatattatg cgcttttgta tttctttcat cactgtcgtt
     4081 agtgtacaat tgactcgacg taaacacgtt aaataacgct tggacatatt taacatcggg
     4141 cgtgttagct ttattaggcc gattatcgtc gtcgtcccaa ccctcgtcgt tagaagttgc
     4201 ttccgaagac gattttgcca tagccacacg acgcctatta attgtgtcgg ctaacacgtc
     4261 cgcgatcaaa tttgtagttg agctttttgg gcctcatggg ccttccgctc actgcccgct
     4321 ttccagtcgg gaaacctgtc gtgccagctg cattaacatg gtcatagctg tttccttgcg
     4381 tattgggcgc tctccgcttc ctcgctcact gactcgctgc gctcggtcgt tcgggtaaag
     4441 cctggggtgc ctaatgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     4501 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     4561 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     4621 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     4681 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     4741 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     4801 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     4861 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     4921 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     4981 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     5041 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     5101 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     5161 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     5221 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     5281 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     5341 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     5401 tgataccgcg agaaccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     5461 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     5521 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     5581 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     5641 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     5701 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     5761 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     5821 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     5881 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     5941 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     6001 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     6061 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     6121 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     6181 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     6241 ttccccgaaa agtgccac
//
